RU168470U1 - DISPOSABLE CASSETTE-ROTOR FOR MICROCENTRIFUGING - Google Patents

Abstract

The utility model relates to analytical biochemistry and can be used for sample preparation and laboratory diagnostics of liquid biological material in various scientific institutions conducting research in the field of biology and medicine, as well as in medical and preventive medical institutions and veterinary medicine. The proposed device is intended for single use, allows you to separate the minimum amounts of biological material into fractions, eliminates the mutual contamination of the analyzed samples and reduces the likelihood of infection of personnel during laboratory diagnostics. 2 ill.

Description

The utility model relates to analytical biochemistry and can be used for sample preparation and laboratory diagnostics of liquid biological material in various scientific institutions conducting research in the field of biology and medicine, as well as in medical and preventive medical institutions and veterinary medicine. The proposed device allows you to divide into fractions the minimum amount of biological material, has a low weight, minimum overall dimensions and is intended for single use.

From the current level of technology, devices for separating liquids into fractions with different densities under the action of centrifugal force / 1 / are known. The essence of the device lies in the fact that for separating a liquid sample inside a test tube (container) for a sample, comprising a separator in which a through channel is formed that extends along the axis and is configured to allow liquid to pass through it, while the separator contains a float and ballast made of according to the density of materials, with part of the float connected to part of the ballast. The axis of the through channel is located in a plane passing at an angle relative to a plane extending vertically from the highest point of the upper outer surface of the float to the lowest point of the outer surface of the ballast and through the center of the separator. This technical solution cannot be used to separate microvolumes (less than 1 ml) of liquids due to the difficult technical design of the separator.

The aim of the invention was to reduce the likelihood of infection of personnel during laboratory diagnostics, especially when working with especially dangerous infections; the exclusion of mutual contamination of the analyzed biological samples; minimize the volume of the test tube for placement and microcentrifugation of a liquid sample of biological material and reduce the volume of hazardous medical waste. In this case, the device should be compatible with automated (robotic) systems and standard centrifuges.

Unlike currently used disposable centrifuge tubes, the use of a disposable rotor cassette with an integrated conical tube in the neck of the tube (well) and a drain (pocket) eliminates the possibility of uncontrolled spilling of liquid from the well.

The presence of a built-in conical tube and outlet (pocket) is an essential feature, since without them it is impossible to achieve the above technical result, which is manifested in a decrease in the probability of infection of personnel during laboratory diagnostics, as well as in the exclusion of mutual contamination of the analyzed biological fluids.

A brief description of the drawings of the proposed technical solution is shown in FIG. 1 and FIG. 2. These options do not limit the proposed utility model, are alternative or combined.

In FIG. 1 shows a schematic illustration of a rotor with a disposable cartridge for microcentrifugation of biological fluids:

A) top view.

B) incision.

In FIG. 2 shows the options for holes with a tap (pocket).

In FIG. 1 and 2 the notation is introduced:

1 - cassette;

2 - hole;

3 - tap (pocket);

4 - rotor;

5 - the central lock of the rotor;

6 - neck of the hole;

7 - solid precipitate (formed elements of blood);

8 - supernatant (blood plasma).

The essence of the utility model of the inventive device for microcentrifugation of biological fluids is illustrated by the graphic materials shown in FIG. 1 and FIG. 2, which provides a specific, but not limiting utility model, the shape of the cassette-rotor.

The cassette-rotor (1) consists of a blank, in particular, in the form of a disk, in the inner volume of which there are holes (2). To collect solid sediment under the action of centrifugal force, a tap (pocket) is provided (3) connected to the hole in a perpendicular plane relative to the axis of symmetry. The cartridge is mounted on the rotor (4) and is held on its surface by a central lock (5) or otherwise. To prevent uncontrolled spillage of the liquid, a tube is mounted in the neck of the hole. The through channel of the tube in a section perpendicular to the transverse axis has a conical shape. The through channel of the tube is located in the device so that the cone of the hole is directed towards the hole.

Wells are filled with samples of the analyzed fluid, for example, with the blood of an animal, and under the action of centrifugal force, the blood cells (red blood cells, white blood cells and platelets) are separated or a solid precipitate is separated (7), which settles in the outlet (pocket), and only blood plasma remains in the hole ( supernatant) (8). Pure blood plasma is taken for further research, and a disposable solid-rotor cassette is sent to a container for storing medical waste.

The main technical result is achieved by using instead of centrifuge tubes a disposable rotor cartridge, in the inner volume of which there are holes with a tap (pocket) for trapping solid sediment and a neck that prevents spilling of the sample. The mouth of the hole to prevent spillage has a conical tube directed into the hole.

In a preferred embodiment, the inventive device can be used in an automated immunochromatographic analysis system and other types of bioanalysers as a disposable consumable for obtaining supernatant after centrifugation. The use of the inventive device in a preferred embodiment reduces the number of additional operations (manipulations), reduces the time of research, prevents personnel from contacting the sample and mutual contamination of the analyzed samples.

Information sources:

/ 1 / RU 2015111344 ⁽¹³⁾ A, Crawford, Jameson W. (US) et al. A device for separating a fluid into phases with different densities (options). Convention Priority Date 05/15/2009 US 61 / 178,599.

Claims (1)

A cassette-rotor for microcentrifugation, consisting of a blank in the form of a disk, in the inner volume of which there are holes for collecting supernatant and bends-pockets for collecting solid sediment under the action of centrifugal force, connected to the holes in a perpendicular plane relative to the center of symmetry, characterized in that there is a built-in tube in the neck of the hole to prevent spillage of liquid from the hole, which, in a section perpendicular to the transverse axis, has a conical shape and is positioned so that mustache opening was directed towards the hole.

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