RS53441B

RS53441B - Gliphosate tolerant sugar beet

Info

Publication number: RS53441B
Application number: YU20050373A
Authority: RS
Inventors: Josef Kraus; Elke Sauerbrey; Reinhard Nehls; Andreas Loock; Rudolf Jansen
Original assignee: Kws Saat Ag
Priority date: 2003-02-20
Filing date: 2004-02-17
Publication date: 2014-12-31
Also published as: ES2391090T3; CA2502981A1; EP1597373B1; JP5586122B2; PT1597373E; EA011923B1; PL214713B1; CA2502981C; RS20050373A; CN102391988A; EA200501096A1; JP2006518205A; PL377934A1; EP1597373A1; SI1597373T1; DK1597373T3; WO2004074492A1

Abstract

Glifozatno tolerantna biljka šećerna repa, naznačena time što:a) je šećerna repa dobijena iz semena deponovanog kod NCIMB pod pristupnim brojem NCIMB 41158 i NCIMB 41159, i/ilib) fragment DNK sa između 630-700 bp od genomske DNK biljke šećerne repe, njenih delova ili semena, može biti amplifikovan korišćenjem polimerazne lančane reakcije sa prvim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 1 i sa drugim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 2, pri čemu fragment DNK pokazuje najmanje 95 % istovetnosti sa nukleotidnom sekvencom SEQ ID NO: 13, i/ilic) fragment DNK sa 3500-3900 bp od genomske DNK biljke šećerne repe, njenih delova ili semena, može biti amplifikovan korišćenjem polimerazne lančane reakcije sa prvom prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 3 i sa drugim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 4, pri čemu fragment DNK pokazuje najmanje 95 % istovetnosti sa nukleotidnom sekvencom SEQ ID NO: 6, i/ilid) fragment DNK sa 270-300 bp od genomske DNK biljke šećerne repe, njenih delova ili semena, može biti amplifikovan korišćenjem polimerazne lančane reakcije sa prvom prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 7 i sa drugim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 8, pri čemu fragment DNK pokazuje najmanje 95 % istovetnosti sa nukleotidnom sekvencom SEQ ID NO: 11, i/ilie) fragment DNK sa 710-790 bp od genomske DNK biljke šećerne repe, njenih delova ili semena, može biti amplifikovan korišćenjem polimerazne lančane reakcije sa prvom prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 9 i sa drugim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 10, pri čemu fragment DNK pokazuje najmanje 95 % istovetnosti sa nukleotidnom sekvencom SEQ ID NO: 12, i/ilif) fragment DNK sa 990-1100 bp od genomske DNK biljke šećerne repe, njenih delova ili semena, može biti amplifikovan korišćenjem polimerazne lančane reakcije sa prvom prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 14 i sa drugim prajmerom koji ima nukleotidnu sekvencu SEQ ID NO: 16, pri čemu fragment DNK pokazuje najmanje 95 % istovetnosti sa nukleotidnom sekvencom SEQ ID NO: 17.Prijava sadrži još 8 patentnih zahteva.Glyphosate tolerant sugar beet plant: a) is sugar beet obtained from seeds deposited with NCIMB under accession number NCIMB 41158 and NCIMB 41159, and / or b) a DNA fragment with between 630-700 bp from the genomic DNA of a sugar beet plant, its parts or seeds, can be amplified using a polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 1 and with a second primer having a nucleotide sequence of SEQ ID NO: 2, wherein the DNA fragment shows at least 95% identity with the nucleotide sequence of SEQ ID NO: 13, and / or) a 3500-3900 bp DNA fragment from the genomic DNA of a beet plant, parts or seeds thereof, can be amplified using a polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 3 and with the second a primer having the nucleotide sequence of SEQ ID NO: 4, wherein the DNA fragment shows at least 95% identity with the nucleotide sequence of SEQ ID NO: 6, and / ylid) a DNA fragment with and 270-300 bp of the genomic DNA of the sugar beet plant, portions or seeds thereof, can be amplified using a polymerase chain reaction with the first primer having the nucleotide sequence of SEQ ID NO: 7 and with the second primer having the nucleotide sequence of SEQ ID NO: 8. wherein the DNA fragment shows at least 95% identity to the nucleotide sequence of SEQ ID NO: 11, and / or e) a DNA fragment of 710-790 bp from the genomic DNA of the sugar beet plant, portions or seeds thereof, can be amplified using the polymerase chain reaction with the first a primer having the nucleotide sequence of SEQ ID NO: 9 and with another primer having the nucleotide sequence of SEQ ID NO: 10, wherein the DNA fragment shows at least 95% identity to the nucleotide sequence of SEQ ID NO: 12, and / or ilif) a DNA fragment of 990 -1100 bp from the genomic DNA of a sugar beet plant, its parts or seeds, can be amplified using a polymerase chain reaction with a first primer having the nucleotide sequence of SEQ ID NO: 14 and with another primer having the nucleotide sequence of SEQ ID NO: 16, wherein the DNA fragment shows at least 95% identity with the nucleotide sequence of SEQ ID NO: 17. The application contains 8 more claims.