RO125754B1 - Mouthwash based on natural active principles and process for preparing the same - Google Patents

Description

The present invention relates to a composition for mouthwater based on natural active principles, intended for the care of the oral cavity, and to a process for its preparation.

Many mouthwatering compositions are known for the care of the oral cavity and for the treatment or prevention of its various conditions.

Thus, from the patent R0114403 an antiseptic mouth water, consisting of

6 .. .7% concentrated ethyl alcohol, 12-13% propylene glycol, 0.001-0.10% propolis, 0.003 ... 0.35% cinnamic aldehyde, 0.003 ... 0.35% alkyl chloride dimethylbenzylammonium and distilled water up to 100%, the percentages being expressed by weight. This drug formula has been shown to be particularly active against microorganisms such as bacteria, yeasts and viruses. Control tests performed on representative germs commonly involved in human and animal pathology, such as staphylococci, streptococci, salmon, colibacilli and klebsiele, have shown the action of suppressing microorganisms, which include both gram positive and gram negative germs, yeasts and viruses.

From patent RO 103501 there is known a concentrate of medicinal mouth water based on herbal extracts, with antimicrobial, hemostatic, healing and inhibiting effects of caryogenic oral flora, consisting of 15 parts Symphyti tincture, 15 parts Calendulae tincture, 25 parts Tincture Salicariae, 15 parts Millefollii tincture, 10 parts Cynosbati tincture, 10 parts Hypophae tincture, 5 parts Propolis tincture, 30% active principles expressed in crisis and one part Aetheroleum Salviae, 1 part Aetheroleum Carvi, 1 part AetheroleumCitri, 1 part Aetheroleum Zhymi 1 part Anetol, the parts being expressed by weight. Mouthwater concentrate has a wide range of use, targeting both children and adults, is well tolerated and does not produce side effects such as staining the tooth surface, toxic or irritating effects.

There is also known mouth water produced by the Apicol Institute, which is presented in the form of an alcoholic solution containing propolis, cinnamon, mint and eucalyptus, with a pleasant taste and which gives a cooling sensation (www.pcfarm.ro) .

From the BG patent 62510 - B1, a mouth water composition is known as a hydroalcoholic solution, containing 100 ... 120 g / l ethyl alcohol, 5 ... 20 g / l calcium and aluminum lactate, 0.5 ... 1.0 g / l benzoic acid, 100 ... 150 mg / l saccharine, 30 ... 50 mg / l peppermint oil or

50 .. .30 mg / l basil oil, dyes 15 ... 30 mg / l and sodium carbonate up to pH 6.0 ... 6.5.

The technical problem that the present invention aims to solve is to establish a composition of mouth water with a wide range of action and without side effects. The proposed solution consists of a composition of mouthwater based on natural principles of plants, conifer resin and propolis.

Therefore, in a first aspect the invention relates to a mouth water based on plant extracts and tincture of propolis, consisting of peppermint hydroalcoholic extract (Mentha piperita) 8 ... 12%, high-speed hydroalcoholic extract ( Agrimonia eupatoria) 8 ... 12%, sage hydroalcoholic extract (Salvia officinalis) 18 ... 22%, chicory hydroalcoholic extract (Cichorium intybus) 13 ... 17%, nalba hydroalcoholic extract (Malva glabra) 18. ..22%, propolis tincture 18 ... 22%, glycerin 1.5 ... 2.5%, sodium bicarbonate 0.5 ... 1.5% and hydroalcoholic extract of coniferous resin 1.5 ... 2.5%, the percentages being expressed by weight.

The invention also relates to a process for preparing the mouth water defined above, in which, in the first phase, a solution of sodium bicarbonate in distilled water is prepared, by mixing at a temperature of 35 ° C until dissolution, In the second phase, a mixture of glycerin, sodium bicarbonate solution of phase 1 and propolis tincture is prepared, by mixing at 38 ... 40 ° C for 60 minutes, in the third phase a mixture of hydroalcoholic extracts of peppermint (Mentha piperita), sea urchin (Agrimonia eupatoria), sage (Salvia officinalis), chicory (Cichorium intybus) and nalba (Malva glabra), and

RO 125754 Β1 hydroalcoholic extract of coniferous resin, by stirring for 60 minutes at a temperature of 1

38 ... 40 ° C, in the fourth phase, the mixture obtained in phase 2 is mixed with the hydroalcoholic mixture in phase 3, by stirring at a speed of 200 rpm / min, for 60 minutes at temperature 3 of 38 ... 40 ° C, then the resulting mixture is filtered, and in the clear solution obtained is added ethyl alcohol of 96 °, until an alcoholic solution of 18 ... 20 ° is obtained. 5

The mouth water according to the invention has anti-infectious, antiseptic, antispastic, astringent, healing, local hemostatic, tonic and stimulant, slightly analgesic, 7 anti-inflammatory actions. It also inhibits the development of Candida Albicans, thus being useful for the treatment and prevention of oral candidiasis. 9

The mouthwash according to the invention has the following advantages:

1. It protects the teeth and gums, and is a disinfectant and mouth freshener; 11

2. being prepared based on natural active principles, it is free of toxicity and unwanted side effects; 13

3. It is useful for the prevention and treatment of dental abscesses, tonsillitis, pharyngitis, gingivitis, mouth sores and other inflammations of the mouth mucosa. 15 below are the methods of preparation and the characteristics of the alcoholic extracts, tinctures and compounds used for the mouth water according to the invention. 17

The peppermint hydroalcoholic extract is obtained by macerating the peppermint leaves (Mentha piperita) in a hydroalcoholic mixture, as follows: in a closed vessel, ethyl alcohol 19 of 40 ... 45 ° is introduced. over which minced mint leaves are added, left to macerate for a minimum of 15 days, stirred daily, then filtered for separation of the extract, which is inserted into sealed glass jars and stored until use.

The main components of the extract are: menthol-rich volatile oil, polyphenolic acids 23 (caffeine, chlorogenic, ferulic, coumaric, labiathenic), flavonoids (apigenin, diosmetin, luteolin, etc.), tannins, carotenoids, vitamin C, nicotinic acid, mineral salts . 25

Action and uses: spasmolytic on the smooth muscle of the digestive tract, antiviral, antimicrobial, digestive antiseptic, diuretic, collagen, carminative, antidiarrheal, moderate sedative, moderate analgesic, strengthening of the nervous system, invigorating. Peppermint leaves are used in hepato-biliary disorders, dyspeptic syndromes, nausea, 29 vomiting, respiratory infections, colds, dysmenorrhea. The hydroalcoholic extract of peppermint leaves improves the lesions of the oral cavity, invigorates and refreshes the breath. 31

The high-speed hydroalcoholic extract is obtained by macerating the aerial part of the Agrimonia eupatoria plant (botanical name: Eupatori herba) in hydroalcoholic mixture 33 as follows: in a closed vessel, ethyl alcohol of 40 ... 45 ° is introduced, over which the part is added aerial of the plant, harvested during flowering, minced, left to macerate for a minimum of 35 15 days, stirred daily, then filtered for separation of the extract, which is inserted into sealed glass jars and kept until use. 37

The main components: tannins (galotanin, elagitanin), asparagine and luteoline glycosides, cvercitine, hyperin, rutoside, traces of volatile oil, bitter substances, ursolic acid, nicotinic acid, vitamin C, vitamin K, silicon compounds.

Action and uses: cholera, collagen, astringent, anticatalatal, stimulates 41 gastrointestinal secretions, stimulates appetite, reduces mucosal inflammation. Externally, the product is used to fight gingivitis and stomatitis, to treat inflammation 43 in the mouth and throat (pharyngitis, tonsillitis), etc.

The sage hydroalcoholic extract is a maceration of the flowering aerial part of the plant 45

Salvia officinalis (Labiatae) in a hydroalcoholic mixture that is obtained as follows: in a closed vessel, ethyl alcohol of 40 ... 45 ° is introduced, over which is added the aerial part of the plant, harvested 47

EN 125754 Β1 during flowering, minced, left to macerate for a minimum of 15 days, stirred daily, then filtered to separate the extract, which is inserted into sealed glass jars and kept until use.

The product aids digestion, increases the body's resistance to infections, reduces excessive sweating and has tonic action, improving nervous asthenia, physical and intellectual overload. In the form of a gargle, the product ameliorates the lesions of the oral cavity (aphthus, gingivitis, stomatitis, dental abscesses).

The main components of the sage hydroalcoholic extract are the following: etheric oil (0.5 ... 2.5%), composed of salven, pinene, camphor, cineole, borneol, thionone, desalven esters and sevecepene; tannins (5 ... 13%); love principle; ursolic acids (1 ... 2%) and oleanolic acids; flavonoid substances; rosmarinic acid (2%), caffeic, chlorogenic, fumaric, glycolic; vitamins (B1, C, nicotinic acid); mineral substances (K, Ca, P, Mg, Na, Fe, Mn, Zn, Cu, Mo).

Action and uses: spasmolytic, antiseptic, astringent, stimulates the secretion of the bile, promotes the elimination of intestinal gas, promotes the process of epithelialization and healing of wounds, balances the metabolism, regulates the menstrual cycle, improves the menopause disorders.

Salvia, in the form of an infusion or hydroalcoholic extract, is used in hypoacid gastritis, gastric atony, biliary dyskinesia, cycle disorders.

The plant is also used externally, in the form of concentrated infusion (3 teaspoons of herbs in a cup with boiling water) or hydroalcoholic extract for gargle in case of gingivitis, aphthus, stomatitis, dental abscesses, etc.

In some countries, sage leaves are used in food, to spice up food.

The hydroalcoholic extract of chicory is obtained by macerating the aerial part of the plant Cichorium intybus (botanical name: Cichoriiherba) in hydroalcoholic mixture, as follows: in a closed vessel ethyl alcohol of 40 ... 45 ° is introduced, over which the aerial part of The plant, harvested during flowering, minced, is left to macerate for a minimum of 15 days, stirred daily, then filtered to separate the extract, which is inserted into sealed glass jars, until use.

Main components: sescviterpenes (sescviterpenic lactones - lactucin, lactucopicrin, cycloroidides B and C, soncuzides C), hydroxycumarines (including umbeliferones), flavonoids (including hyperosides), inulin, arginine, choline, caffeic acid and derivatives, cyclorine, polyurine, levulin mineral substances.

Action and uses: general tonic, remineralizing, anti-anemic, aperitive, stomachic, depurative, cholera and moderate collagen, antiexudative, diuretic, moderate laxative, vermifuge, febrifuge, moderate sedative, antiarrhythmic and hypotensive, hypoglycaemic. The plant is used in anorexia, dyspeptic syndromes, asthenia, anemia, biliary insufficiency, liver colic, hepatic and splenic congestion, digestive atony, dermatosis, gout, renal and bladder lithiasis, urinary infections.

The hydroalcoholic extract of honeysuckle is obtained by macerating in the hydroalcoholic mixture of flowers and leaves of honeysuckle Malva glabra (botanical name: Malve glabre folium etflos), thus: in a closed vessel is introduced ethyl alcohol of 40 ... 45 °, above which add minced flowers and leaves, leave to macerate for a minimum of 15 days, shake daily, then filter to separate the extract, which is inserted into sealed glass jars until use.

The main components: the leaves contain mucilages, tannins, vitamin A,

B1, B2, C and mineral compounds. The flowers contain mucilages, anthocyanosides that have gianine malvidol.

RO 125754 Β1

Action and uses: emollient, soothing, expectorant, anti-inflammatory, healing 1 and protective. The active principles contained in the plant, especially the mucilages, relax the tissues, diminish the inflammatory state until it disappears, favor the expectoration, 3 diminish the cough access, promote the healing process and epithelialization of the wounds. Nalba extracts are used externally for the treatment of dental abscesses, tonsils and 5 for the care of the oral cavity.

Propolis tincture 7

Propolis is a yellow-brown to brown resinous substance, collected by working bees from different parts of trees or shrubs (leaf buds, 9 trunk lesions, etc.).

Propolis tincture is obtained by macerating propolis in 70 ° alcohol for 11

10 ... 14 days, in closed pots, at room temperature, shaking 1 ... 2 times a day. After maceration, the extract liquid is separated and left to settle for 5-6 days, then filtered. The clear filtrate is inserted into glass jars, where it is stored until use.

The main components: flavonoids (most of them glycosides), the main substances responsible for the biological activity of propolis (galangin, kaempferol, quercetin, pinocembrin, pinostrobin, pinobanksin); phenolic acids and esters thereof (cinnamic alcohol 17, cinnamic acid, vanillin, benzyl alcohol, benzoic acid, caffeic acid, ferulic acid), beeswax, essential oil, pollen (proteins, amino acids, mainly arginine and proline) , 19 other organic compounds (ketones, lactones, quinones, steroids, benzoic acid, vitamins and sugars), mineral substances in which Fe and Zn have an important weight. 21

Action and uses: reduced nutrition (due to small amounts of protein, amino acids, minerals, sugars, vitamins), antimicrobial (checked for 16 bacterial-23 strains), antifungal, antiviral (including anti-herpetic and anti-influenza), promotes vitamin C uptake ( due to dihydroflavonoids), antioxidant, tissue regenerator, anti-inflammatory, slightly anesthetic, immunostimulant, cardioprotective (antihypertensive, sedative, capillary-protective, antihyperlipidemic), hepatoprotective, protects teeth and gums. Anti-tumor action of propolis was also reported, due to the presence of quercetin, caffeic acid and clerodan diterpenoid. The latter has been shown to have selective cytotoxic effect 29 on tumor cells.

For its properties, propolis is used in respiratory infections (pharyngitis, 31 chronic bronchitis, rhinopharyngolaryngitis, bronchial catarrh, rhinitis), viral infections (influenza, herpes etc.), skin infections, dental and oral treatments (gingivitis, inflammation of the mouth mucosa, etc.). .), 33 immunodeficiency, inflammation of the joints, etc.

Hydroalcoholic extract of coniferous resin is prepared as follows: in a closed vessel 35 is introduced ethyl alcohol of 40 ... 45 °, on top of which is added fir, pine or spruce resin, left to macerate for a minimum of 15 days, it is stirred daily, then filtered to separate the extract 37, which is inserted into sealed glass vessels, until used, the following are examples of embodiments of the invention which are illustrative, and not limiting. 39

Example 1 In a reaction vessel fitted with a stirrer, thermometer, the heating-cooling jacket, 41 g of sodium bicarbonate and 100 ml of distilled water were introduced and mixed at 35 ° C until dissolved. Separately, in another reaction vessel provided with a stirrer, thermo- 43 meters, heating-cooling jacket, 1.8 g of glycerine, 100 g of aqueous sodium bicarbonate solution and 22 g of propolis tincture are introduced, and mix at a speed of 200 rpm, 45 for 30 minutes, at 35 ... 40 ° C. Separately, place in a mixing vessel g of peppermint hydroalcoholic extract (Mentha piperita), 12 g of high-viscous hydroalcoholic extract 47 (Agrimonia eupatoria), 18 g of sage hydroalcoholic extract (Salvia officinalis), 17 g of

RO 125754 Β1 chicory hydro-alcoholic extract (Cichorium intybus) and 18 g of nalba hydroalcoholic extract (Malva glabra), 1.8 g of coniferous resin hydroalcoholic extract, and stirred for 90 minutes, at 35 ... 40 ° C, and the mixture thus obtained is added to the mixture of aqueous solution of sodium bicarbonate, tincture of propolis and glycerin, and is homogenized by mixing at a speed of 200 rpm, for 60 minutes, at a temperature of 38 ° C. The resulting mixture is then filtered and 10 g of 96 ° ethyl alcohol is added to the clear solution obtained. The solution thus obtained is packed in sealable vials.

Example 2 In a reaction vessel fitted with a stirrer, thermometer, heating-cooling jacket, 1 g of sodium bicarbonate and 100 ml of distilled water are introduced, by mixing at 35 ° C, until dissolved. Separately, in another reaction vessel provided with agitator, thermometer, heating-cooling jacket, add 2.2 g of glycerin, 100 g of aqueous sodium bicarbonate solution and 18 g of propolis tincture, and mix. at a speed of 200 rpm for 30 minutes at a temperature of 35 ... 40 ° C. Separately, add 12 g of peppermint hydroalcohol extract (Mentha piperita), 8 g of high-speed hydroalcoholic extract (Agrimonia eupatoria), 22 g of sage hydroalcoholic extract (Salvia officinalis), 13 g of chicory hydroalcoholic extract (Cichorium intybus) and 22 g nalba hydroalcoholic extract (Malva glabra), 2.2 g of coniferous resin hydroalcoholic extract, and stirred for 90 min at 35 ... 40 ° C, and the mixture thus obtained is added to the mixture of aqueous solution of sodium bicarbonate, tincture of propolis and glycerin, and is homogenized by mixing at a speed of 200 rpm, for 60 minutes, at a temperature of 38 ° O. The resulting mixture is then filtered and 10 g of 96 ° ethyl alcohol is added to the clear solution obtained. The solution thus obtained is packed in sealable vials.

The physico-chemical characteristics of the mouthwater according to the invention are shown in the table below:

Characteristic Value appearance Clear liquid Color Yellow brown Smell Characteristic, aromatic Taste Characteristic PH 5.0 ... 6.5

Claims (2)

claims 1. Mouth water based on natural principles from plant extracts and tincture of 3 propolis, characterized in that it consists of peppermint hydroalcoholic extract (Mentha piperita) 8 ... 12%, high-speed hydroalcoholic extract (Agrimonia eupatoria) ) 8 ... 12%, 5 sage hydroalcoholic extract (Salvia officinalis) 18 ... 22%, chicory hydroalcoholic extract (Cichorium intybus) 13 ... 17%, nalba hydroalcoholic extract (Malva glabra) 18.. .22%, 7 dyes of propolis 18 ... 22%, glycerin 1.5 ... 2.5%, sodium bicarbonate 0.5 ... 1.5%, hydroalcoholic extract of coniferous resin 1.5 ... 2.5%, the percentages being expressed by weight. 9 A process for preparing the mouthwater as defined in claim 1, characterized in that in the first phase a solution of sodium bicarbonate in distilled water is prepared, by mixing at 35 ° C, until dissolved; In the second phase, a mixture of glycerin, sodium bicarbonate solution of phase 1 and propolis tincture is prepared, by mixing at a temperature of 38 ... 40 ° C for 60 minutes; In the third phase, a mixture of peppermint (Mentha piperita), high turmeric (Agrimonia eupatoria), 15 sage (Salvia officinalis), chicory (Cichorium intybus), chicory (Malva glabra) and coniferous resin is prepared by stirring, by stirring. for 60 min at 38 ... 40 ° C; In the fourth phase, the mixture obtained in the second phase is mixed with the hydroalcoholic mixture in the third phase, by stirring at a speed of 200 rpm for 60 minutes, at a temperature of 38 ... 40 ° C, then 19 the resulting mixture is filtered, and in the clear solution obtained is added ethyl alcohol of 96 °, until an alcoholic solution of 18 ... 20 ° is obtained. 21