PT904412E - Metedo para amplificacao de polinucleotidos - Google Patents
Metedo para amplificacao de polinucleotidosInfo
- Publication number
- PT904412E PT904412E PT97952592T PT97952592T PT904412E PT 904412 E PT904412 E PT 904412E PT 97952592 T PT97952592 T PT 97952592T PT 97952592 T PT97952592 T PT 97952592T PT 904412 E PT904412 E PT 904412E
- Authority
- PT
- Portugal
- Prior art keywords
- polynucleotide sequence
- target polynucleotide
- oligonucleotide
- oligonucleotide primer
- polynucleotides
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3313796P | 1996-12-20 | 1996-12-20 | |
US08/965,492 US6482590B1 (en) | 1996-12-20 | 1997-11-06 | Method for polynucleotide amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
PT904412E true PT904412E (pt) | 2002-04-29 |
Family
ID=26709340
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT97952592T PT904412E (pt) | 1996-12-20 | 1997-12-17 | Metedo para amplificacao de polinucleotidos |
Country Status (10)
Country | Link |
---|---|
US (1) | US6482590B1 (pt) |
EP (1) | EP0904412B1 (pt) |
JP (1) | JP2002503089A (pt) |
AT (1) | ATE206766T1 (pt) |
CA (1) | CA2246225A1 (pt) |
DE (1) | DE69707284T2 (pt) |
DK (1) | DK0904412T3 (pt) |
ES (1) | ES2165634T3 (pt) |
PT (1) | PT904412E (pt) |
WO (1) | WO1998028443A1 (pt) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7070962B1 (en) * | 1996-02-01 | 2006-07-04 | Aventis Pharmaceuticals Inc. | Positive controls in polynucleotide amplification |
CA2257109C (en) * | 1996-06-04 | 2009-10-06 | University Of Utah Research Foundation | Monitoring hybridization during pcr |
FR2779154B1 (fr) * | 1998-05-27 | 2002-07-12 | Bio Merieux | Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre |
US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
US6692918B2 (en) | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
DK1218542T3 (da) | 1999-09-13 | 2004-08-02 | Nugen Technologies Inc | Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser |
US6274353B1 (en) | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
WO2001029212A1 (en) * | 1999-10-19 | 2001-04-26 | Enchira Biotechnology Corporation | Methods for chimeragenesis of whole genomes or large polynucleotides |
US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
EP1427847B1 (en) | 2000-12-13 | 2010-07-28 | Nugen Technologies, Inc. | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
KR20030082535A (ko) | 2001-03-09 | 2003-10-22 | 뉴젠 테크놀로지스 인코포레이티드 | Rna 서열의 증폭을 위한 방법 및 조성물 |
EP1275735A1 (en) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Composition and method for hot start nucleic acid amplification |
EP1615942B1 (en) | 2003-04-01 | 2016-03-16 | Luminex Corporation | Polymerase inhibitor and method of using same |
US7402386B2 (en) | 2003-04-14 | 2008-07-22 | Nugen Technologies, Inc. | Global amplification using random priming by a composite primer |
WO2007008728A2 (en) * | 2005-07-07 | 2007-01-18 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
EP1929046B1 (en) | 2005-09-07 | 2012-07-11 | Nugen Technologies, Inc. | Improved nucleic acid amplification procedure |
DE102005047617A1 (de) * | 2005-10-05 | 2007-04-19 | Qiagen Gmbh | Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft |
WO2007074894A1 (ja) * | 2005-12-28 | 2007-07-05 | School Corporation, Azabu Veterinary Medicine Educational Institution | 標的配列の特異的高感度増幅法 |
JP5558811B2 (ja) * | 2006-06-01 | 2014-07-23 | トリリンク バイオテクノロジーズ | 核酸増幅のための化学的に修飾されたオリゴヌクレオチドプライマー |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
GB2470672B (en) | 2008-03-21 | 2012-09-12 | Nugen Technologies Inc | Methods of RNA amplification in the presence of DNA |
WO2010141390A2 (en) | 2009-06-05 | 2010-12-09 | Life Technologies Corporation | Nucleotide transient binding for sequencing methods |
ES2905448T3 (es) | 2012-05-10 | 2022-04-08 | Massachusetts Gen Hospital | Métodos para determinar una secuencia nucleotídica |
KR102264617B1 (ko) | 2012-06-14 | 2021-06-14 | 라이프 테크놀로지스 코포레이션 | 폴리머라제 연쇄 반응 (pcr)을 위한 신규 조성물, 방법 및 키트 |
EP3099820A4 (en) | 2014-01-27 | 2018-01-03 | The General Hospital Corporation | Methods of preparing nucleic acids for sequencing |
EP3402880B1 (en) | 2016-01-15 | 2024-01-03 | Thermo Fisher Scientific Baltics UAB | Thermophilic dna polymerase mutants |
AU2017328953B2 (en) | 2016-09-15 | 2023-09-14 | Archerdx, Llc | Methods of nucleic acid sample preparation for analysis of cell-free DNA |
WO2018053362A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation |
US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
US10768173B1 (en) | 2019-09-06 | 2020-09-08 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
US10876148B2 (en) | 2018-11-14 | 2020-12-29 | Element Biosciences, Inc. | De novo surface preparation and uses thereof |
US10704094B1 (en) | 2018-11-14 | 2020-07-07 | Element Biosciences, Inc. | Multipart reagents having increased avidity for polymerase binding |
JP7499767B2 (ja) | 2018-12-07 | 2024-06-14 | エレメント バイオサイエンシーズ,インク. | フローセルデバイスおよびその使用 |
CA3122494A1 (en) * | 2018-12-13 | 2020-06-18 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
Family Cites Families (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US5008182A (en) | 1986-01-10 | 1991-04-16 | Cetus Corporation | Detection of AIDS associated virus by polymerase chain reaction |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
WO1986006412A1 (en) | 1985-05-02 | 1986-11-06 | Genetics Institute, Inc. | Process and nucleic acid construct for producing reagent complexes useful in determining target nucleotide sequences |
US4800159A (en) * | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US4994368A (en) | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
US5508178A (en) | 1989-01-19 | 1996-04-16 | Rose; Samuel | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5766849A (en) * | 1989-07-11 | 1998-06-16 | Gen-Probe Incorporated | Methods of amplifying nucleic acids using promoter-containing primer sequence |
US5218088A (en) * | 1989-11-02 | 1993-06-08 | Purdue Research Foundation | Process for preparing dithiophosphate oligonucleotide analogs via nucleoside thiophosphoramidite intermediates |
KR950013953B1 (ko) | 1990-01-26 | 1995-11-18 | 애보트 래보라토리즈 | 리가제 연쇄 반응에 적용가능한 표적 핵산의 증폭 방법 |
US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
CA2080305A1 (en) | 1991-10-11 | 1993-04-12 | Linda M. Western | Method for producing a polynucleotide for use in single primer amplification and phosphorothioate-containing oligonucleotides as primers in nucleic acid amplification |
ES2104160T3 (es) | 1992-07-31 | 1997-10-01 | Behringwerke Ag | Metodo para introducir secuencias definidas en el extremo 3' de polinucleotidos. |
US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
US5733733A (en) | 1992-08-04 | 1998-03-31 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
US5436143A (en) * | 1992-12-23 | 1995-07-25 | Hyman; Edward D. | Method for enzymatic synthesis of oligonucleotides |
JPH07198A (ja) | 1993-04-21 | 1995-01-06 | Toyobo Co Ltd | 核酸増幅反応の増幅産物による汚染を防止する方法 |
US5605796A (en) | 1994-07-19 | 1997-02-25 | Behringwerke Ag | Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates |
ZA956776B (en) | 1994-08-25 | 1996-03-20 | Akzo Nobel Nv | A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay |
US5763594A (en) * | 1994-09-02 | 1998-06-09 | Andrew C. Hiatt | 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds |
US5753439A (en) | 1995-05-19 | 1998-05-19 | Trustees Of Boston University | Nucleic acid detection methods |
CA2176193A1 (en) | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
US5747255A (en) | 1995-09-29 | 1998-05-05 | Lynx Therapeutics, Inc. | Polynucleotide detection by isothermal amplification using cleavable oligonucleotides |
WO1997023646A1 (en) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Detection of differences in nucleic acids |
DE69714526T2 (de) | 1996-02-01 | 2003-03-20 | Aventis Pharmaceuticals Inc., Bridgewater | Positiv kontrollen zur verwendung bei polynukleotid - vervielfältigungen |
WO1997035033A1 (en) * | 1996-03-19 | 1997-09-25 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
-
1997
- 1997-11-06 US US08/965,492 patent/US6482590B1/en not_active Expired - Fee Related
- 1997-12-17 ES ES97952592T patent/ES2165634T3/es not_active Expired - Lifetime
- 1997-12-17 EP EP97952592A patent/EP0904412B1/en not_active Revoked
- 1997-12-17 JP JP52903598A patent/JP2002503089A/ja active Pending
- 1997-12-17 AT AT97952592T patent/ATE206766T1/de not_active IP Right Cessation
- 1997-12-17 CA CA002246225A patent/CA2246225A1/en not_active Abandoned
- 1997-12-17 WO PCT/US1997/023706 patent/WO1998028443A1/en not_active Application Discontinuation
- 1997-12-17 DK DK97952592T patent/DK0904412T3/da active
- 1997-12-17 DE DE69707284T patent/DE69707284T2/de not_active Revoked
- 1997-12-17 PT PT97952592T patent/PT904412E/pt unknown
Also Published As
Publication number | Publication date |
---|---|
WO1998028443A1 (en) | 1998-07-02 |
ATE206766T1 (de) | 2001-10-15 |
CA2246225A1 (en) | 1998-07-02 |
ES2165634T3 (es) | 2002-03-16 |
DE69707284D1 (de) | 2001-11-15 |
DK0904412T3 (da) | 2002-02-11 |
EP0904412B1 (en) | 2001-10-10 |
DE69707284T2 (de) | 2002-10-17 |
EP0904412A1 (en) | 1999-03-31 |
JP2002503089A (ja) | 2002-01-29 |
US6482590B1 (en) | 2002-11-19 |
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