PL450057A1 - Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu - Google Patents

Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu

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Publication number
PL450057A1
PL450057A1 PL450057A PL45005724A PL450057A1 PL 450057 A1 PL450057 A1 PL 450057A1 PL 450057 A PL450057 A PL 450057A PL 45005724 A PL45005724 A PL 45005724A PL 450057 A1 PL450057 A1 PL 450057A1
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PL
Poland
Prior art keywords
egfp
protein
modified
green fluorescent
fluorescent protein
Prior art date
Application number
PL450057A
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English (en)
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PL248452B1 (pl
Inventor
Marcin Olszewski
Katarzyna Szymańska
Emil Pituła
Monika Janik
Dawid NIDZWORSKI
Beata Gromadzka
Original Assignee
Politechnika Warszawska
Instytut Biotechnologii I Medycyny Molekularnej
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Application filed by Politechnika Warszawska, Instytut Biotechnologii I Medycyny Molekularnej filed Critical Politechnika Warszawska
Priority to PL450057A priority Critical patent/PL248452B1/pl
Publication of PL450057A1 publication Critical patent/PL450057A1/pl
Publication of PL248452B1 publication Critical patent/PL248452B1/pl

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/1813—Specific cations in water, e.g. heavy metals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest zmodyfikowane, wzmocnione białko zielonej fluorescencji TST-eGFP-MBL-Ni2+, które ma sekwencję aminokwasową białka eGFP, w której w miejsce sekwencji DGFP obejmującej reszty aminokwasowe od 190 do 193 wstawiono co najmniej jedną, 7 aminokwasową sekwencję SEQ ID NO.1, która wiąże jony niklu. Zgłoszenie ujawnia też konstrukt genetyczny o sekwencji SEQ ID NO.3, którego ekspresja daje produkt w postaci zmodyfikowanego białka TST-eGFP-MBL-Ni2+ o SEQ ID NO.2. Zgłoszenie obejmuje też sposób wytwarzania zmodyfikowanego białka TST-eGFP-MBL-Ni2+ i jego zastosowanie do oznaczania niklu w próbkach ciekłych.
PL450057A 2024-10-17 2024-10-17 Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu PL248452B1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450057A PL248452B1 (pl) 2024-10-17 2024-10-17 Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450057A PL248452B1 (pl) 2024-10-17 2024-10-17 Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu

Publications (2)

Publication Number Publication Date
PL450057A1 true PL450057A1 (pl) 2025-02-17
PL248452B1 PL248452B1 (pl) 2025-12-15

Family

ID=94601209

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450057A PL248452B1 (pl) 2024-10-17 2024-10-17 Zmodyfikowane białko zielonej fluorescencji, sposób jego wytwarzania oraz jego zastosowanie do oznaczania niklu

Country Status (1)

Country Link
PL (1) PL248452B1 (pl)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DE CAROLI, M. ET AL.: "Int J Mol Sci. 2024 May 31; 25(11):6090", DEVELOPMENT OF A WHOLE-CELL SYSTEM BASED ON THE USE OF GENETICALLY MODIFIED PROTOPLASTS TO DETECT NICKEL IONS IN FOOD MATRICES. *
KIM, H. ET AL.: "Appl Microbiol Biotechnol., 2019,103: 6345-6352", HEAVY METAL(LOID) BIOSENSOR BASED ON SPLIT-ENHANCED GREEN FLUORESCENT PROTEIN: DEVELOPMENT AND CHARACTERIZATION. *
LEE, W. ET AL.: "Sensors, 2019, 19(8): 1846", A BIOSENSOR PLATFORM FOR METAL DETECTION BASED ON ENHANCED GREEN FLUORESCENT PROTEIN. *
RAJA, C.E. ET AL.: "Int. J. Environ. Sci. Tech., 2011, 8, 793-798", CONSTRUCTION OF GREEN FLUORESCENT PROTEIN BASED BACTERIAL BIOSENSOR FOR HEAVY METAL REMEDIATION. *

Also Published As

Publication number Publication date
PL248452B1 (pl) 2025-12-15

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