PL431942A1 - Sposób i zestaw do wykrywania pozajelitowych szczepów E. coli patogennych dla drobiu - Google Patents

Sposób i zestaw do wykrywania pozajelitowych szczepów E. coli patogennych dla drobiu

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Publication number
PL431942A1
PL431942A1 PL431942A PL43194219A PL431942A1 PL 431942 A1 PL431942 A1 PL 431942A1 PL 431942 A PL431942 A PL 431942A PL 43194219 A PL43194219 A PL 43194219A PL 431942 A1 PL431942 A1 PL 431942A1
Authority
PL
Poland
Prior art keywords
kit
extraintestinal
poultry
detection
pathogenic
Prior art date
Application number
PL431942A
Other languages
English (en)
Other versions
PL240694B1 (pl
Inventor
Jarosław DASTYCH
Joanna KAZIMIERCZAK
Karolina POSPIECH
Patrycja SOWIŃSKA
Ewelina A. WÓJCIK
Małgorzata STAŃCZYK
Justyna ANDRYSIAK
Dominik Strapagiel
Błażej Marciniak
Paulina BORÓWKA
Marcin Wojciech LIS
Original Assignee
Proteon Pharmaceuticals Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteon Pharmaceuticals Spółka Akcyjna filed Critical Proteon Pharmaceuticals Spółka Akcyjna
Priority to PL431942A priority Critical patent/PL240694B1/pl
Priority to CA3163082A priority patent/CA3163082A1/en
Priority to MX2022006380A priority patent/MX2022006380A/es
Priority to PCT/PL2020/050089 priority patent/WO2021107795A1/en
Priority to EP20845245.8A priority patent/EP4065733B1/en
Priority to US17/779,351 priority patent/US20230287518A1/en
Priority to BR112022010224A priority patent/BR112022010224A2/pt
Publication of PL431942A1 publication Critical patent/PL431942A1/pl
Publication of PL240694B1 publication Critical patent/PL240694B1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób identyfikacji szczepu E.coli patogennego dla ptaków (APEC),charakteryzujący się tym, że a) z badanej próbki materiału biologicznego izoluje się DNA, b) w próbce wyizolowanego DNA bada się obecność: genów wirulencji iroC i hlyF oraz genu odpowiedzialnego za serotyp O78, c) stwierdzenie obecności co najmniej jednego genu spośród wspomnianych genów wirulencji oraz genu odpowiedzialnego za serotyp O78 świadczy o zidentyfikowaniu szczepu APEC w badanej próbce. Zgłoszenie obejmuje także zestaw do identyfikacji szczepu E.coli patogennego dla ptaków (APEC), oraz jego zastosowanie.
PL431942A 2019-11-26 2019-11-26 Sposób i zestaw do identyfikacji szczepu E.coli patogennego dla ptaków PL240694B1 (pl)

Priority Applications (7)

Application Number Priority Date Filing Date Title
PL431942A PL240694B1 (pl) 2019-11-26 2019-11-26 Sposób i zestaw do identyfikacji szczepu E.coli patogennego dla ptaków
CA3163082A CA3163082A1 (en) 2019-11-26 2020-11-26 Method and system for detection of extraintestinal e. coli strains pathogenic to poultry
MX2022006380A MX2022006380A (es) 2019-11-26 2020-11-26 Método y sistema para la detección de cepas extraintestinales de e. coli patógenas para aves de corral.
PCT/PL2020/050089 WO2021107795A1 (en) 2019-11-26 2020-11-26 Method and system for detection of extraintestinal e. coli strains pathogenic to poultry
EP20845245.8A EP4065733B1 (en) 2019-11-26 2020-11-26 Method and kit for detection of extraintestinal e. coli strains pathogenic to poultry
US17/779,351 US20230287518A1 (en) 2019-11-26 2020-11-26 Method and system for detection of extraintestinal e. coli strains pathogenic to poultry
BR112022010224A BR112022010224A2 (pt) 2019-11-26 2020-11-26 Método e sistema para detecção de cepas extraintestinais de e. coli patogênicas para aves de capoeira

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL431942A PL240694B1 (pl) 2019-11-26 2019-11-26 Sposób i zestaw do identyfikacji szczepu E.coli patogennego dla ptaków

Publications (2)

Publication Number Publication Date
PL431942A1 true PL431942A1 (pl) 2021-05-31
PL240694B1 PL240694B1 (pl) 2022-05-23

Family

ID=74195095

Family Applications (1)

Application Number Title Priority Date Filing Date
PL431942A PL240694B1 (pl) 2019-11-26 2019-11-26 Sposób i zestaw do identyfikacji szczepu E.coli patogennego dla ptaków

Country Status (7)

Country Link
US (1) US20230287518A1 (pl)
EP (1) EP4065733B1 (pl)
BR (1) BR112022010224A2 (pl)
CA (1) CA3163082A1 (pl)
MX (1) MX2022006380A (pl)
PL (1) PL240694B1 (pl)
WO (1) WO2021107795A1 (pl)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102546A1 (en) * 1998-02-12 2002-08-01 Nolan Lisa K. Nucleic acid encoding an avian E.coli iss polypeptide and methods of use
US6706475B1 (en) 1998-04-01 2004-03-16 The United States Of America As Represented By The Department Of Health And Human Services Oligonucleotide probes for detecting Enterobacteriaceae and quinolone-resistant Enterobacteriaceae
WO2003095677A1 (en) 2002-05-09 2003-11-20 Medigenes Nucleic acid probes for detection of non-viral organisms
US20100167956A1 (en) 2007-01-08 2010-07-01 Medigenes Co., Ltd. Dna chip for detection of escherichia coli
EP2298798B1 (en) 2009-09-10 2012-05-09 Freie Universität Berlin Identification and characterization of a novel avian pathogenic E. coli (APEC) fimbrial adhesin
US20140193824A1 (en) 2013-01-04 2014-07-10 Evgeni V. SOKURENKO High-Resolution Clonal Typing of Escherichia coli

Also Published As

Publication number Publication date
CA3163082A1 (en) 2021-06-03
US20230287518A1 (en) 2023-09-14
WO2021107795A1 (en) 2021-06-03
MX2022006380A (es) 2022-08-10
EP4065733B1 (en) 2024-05-01
PL240694B1 (pl) 2022-05-23
BR112022010224A2 (pt) 2022-10-25
EP4065733A1 (en) 2022-10-05

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