PL430801A1 - Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej - Google Patents

Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej

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Publication number
PL430801A1
PL430801A1 PL430801A PL43080119A PL430801A1 PL 430801 A1 PL430801 A1 PL 430801A1 PL 430801 A PL430801 A PL 430801A PL 43080119 A PL43080119 A PL 43080119A PL 430801 A1 PL430801 A1 PL 430801A1
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Poland
Prior art keywords
culture
fragments
tissue
treatment
minimum
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PL430801A
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English (en)
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PL240714B1 (pl
Inventor
Sławomir JAROS
Grzegorz SOBIERAJ
Julia Balcerek
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Arthec Spółka Z Ograniczoną Odpowiedzialnością
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Application filed by Arthec Spółka Z Ograniczoną Odpowiedzialnością filed Critical Arthec Spółka Z Ograniczoną Odpowiedzialnością
Priority to PL430801A priority Critical patent/PL240714B1/pl
Priority to US17/632,141 priority patent/US20220265899A1/en
Priority to PT207502709T priority patent/PT4007615T/pt
Priority to KR1020227006953A priority patent/KR20220042423A/ko
Priority to PCT/EP2020/071826 priority patent/WO2021023718A1/en
Priority to DK20750270.9T priority patent/DK4007615T3/da
Priority to JP2022533249A priority patent/JP2022543174A/ja
Priority to ES20750270T priority patent/ES2967034T3/es
Priority to FIEP20750270.9T priority patent/FI4007615T3/fi
Priority to CN202080069155.4A priority patent/CN114466923B/zh
Priority to CA3149427A priority patent/CA3149427C/en
Priority to IL290282A priority patent/IL290282B2/en
Priority to EP20750270.9A priority patent/EP4007615B8/en
Priority to HUE20750270A priority patent/HUE064647T2/hu
Publication of PL430801A1 publication Critical patent/PL430801A1/pl
Publication of PL240714B1 publication Critical patent/PL240714B1/pl
Priority to JP2025086260A priority patent/JP2025122124A/ja

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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A61L27/3612—Cartilage, synovial fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
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    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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Abstract

Przedmiotem wynalazku jest sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej, polegający na tym, że pobraną tkankę chrzęstną szklistą tnie się na co najmniej dwa fragmenty tkanki, a następnie fragmenty te układa się na płytce hodowlanej obok siebie w medium hodowlanym zawierającym minimum 2 mM glutaminy, minimum 2% FBS, minimum 4.5 g/l glukozy. Kształt fragmentów tkanek dopasowuje się co najmniej na jednym odcinku krawędzi każdego fragmentu tak aby po ułożeniu fragmentów blisko siebie przestrzeń pomiędzy zbliżonymi co najmniej dwoma fragmentami tkanki była jak najmniejsza i tak aby wypełniana fragmentami tkanek przestrzeń płytki hodowlanej była jak najmniejsza. Tak dopasowuje się krawędzie aby w trakcie hodowli złączyć ze sobą fragmenty na co najmniej jednym odcinku stycznym. Do hodowli dodaje się zawiesiny chondrocytów i prowadzi się hodowlę komórkową fragmentów tkanki z chondrocytami. Medium hodowlane zmieniania się w ustalonym czasie, dodając do medium hodowlanego chondrocyty, przy czym hodowlę prowadzi się aż do uzyskania złączenia fragmentu tkanki na co najmniej części odcinka stycznego.
PL430801A 2019-08-02 2019-08-02 Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej PL240714B1 (pl)

Priority Applications (15)

Application Number Priority Date Filing Date Title
PL430801A PL240714B1 (pl) 2019-08-02 2019-08-02 Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej
ES20750270T ES2967034T3 (es) 2019-08-02 2020-08-03 Un método de cultivo in vitro de condrocitos y cartílagos para obtener material para el tratamiento de defectos del cartílago articular
FIEP20750270.9T FI4007615T3 (fi) 2019-08-02 2020-08-03 Menetelmä kondrosyytti- ja rustoviljelyyn in vitro, materiaalin saamiseksi nivelruston vaurioiden hoitoon
KR1020227006953A KR20220042423A (ko) 2019-08-02 2020-08-03 관절 연골 결손을 치료하기 위한 물질을 얻기 위한 시험관내 연골세포 및 연골 배양 방법
PCT/EP2020/071826 WO2021023718A1 (en) 2019-08-02 2020-08-03 A method of in vitro chondrocyte and cartilage culture to obtain material for the treatment of articular cartilage defects
DK20750270.9T DK4007615T3 (da) 2019-08-02 2020-08-03 Fremgangsmåde til in vitro dyrkning af kondrocytter og bruskvæv til opnåelse af materiale til behandling af ledbruskdefekter
JP2022533249A JP2022543174A (ja) 2019-08-02 2020-08-03 関節軟骨欠損症の処置用の材料を得るための、軟骨細胞および軟骨のｉｎ ｖｉｔｒｏ培養の方法
US17/632,141 US20220265899A1 (en) 2019-08-02 2020-08-03 A method of in vitro chondrocyte and cartilage culture to obtain material for the treatment of articular cartilage defects
PT207502709T PT4007615T (pt) 2019-08-02 2020-08-03 Método de cultura in vitro de condrócitos e cartilagem para obtenção de material para tratamento de defeitos da cartilagem articular
CN202080069155.4A CN114466923B (zh) 2019-08-02 2020-08-03 一种体外培养软骨细胞和软骨以获得治疗关节软骨缺损材料的方法
CA3149427A CA3149427C (en) 2019-08-02 2020-08-03 A method of in vitro chondrocyte and cartilage culture to obtain material for the treatment of articular cartilage defects
IL290282A IL290282B2 (en) 2019-08-02 2020-08-03 Method for culturing chondrocytes and cartilage to obtain material for treating articular cartilage disorders
EP20750270.9A EP4007615B8 (en) 2019-08-02 2020-08-03 A method of in vitro chondrocyte and cartilage culture to obtain material for the treatment of articular cartilage defects
HUE20750270A HUE064647T2 (hu) 2019-08-02 2020-08-03 In vitro kondrocita és porc tenyésztési eljárás ízületi porc károsodások kezelésére szolgáló anyag elõállítására
JP2025086260A JP2025122124A (ja) 2019-08-02 2025-05-23 関節軟骨欠損症の処置用の材料を得るための、軟骨細胞および軟骨のｉｎ ｖｉｔｒｏ培養の方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL430801A PL240714B1 (pl) 2019-08-02 2019-08-02 Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej

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PL430801A1 true PL430801A1 (pl) 2021-02-08
PL240714B1 PL240714B1 (pl) 2022-05-23

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PL430801A PL240714B1 (pl) 2019-08-02 2019-08-02 Sposób prowadzenia hodowli komórkowej chondrocytów in vitro do uzyskiwania materiału do leczenia ubytków chrząstki stawowej

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US (1) US20220265899A1 (pl)
EP (1) EP4007615B8 (pl)
JP (2) JP2022543174A (pl)
KR (1) KR20220042423A (pl)
CN (1) CN114466923B (pl)
DK (1) DK4007615T3 (pl)
ES (1) ES2967034T3 (pl)
FI (1) FI4007615T3 (pl)
HU (1) HUE064647T2 (pl)
IL (1) IL290282B2 (pl)
PL (1) PL240714B1 (pl)
PT (1) PT4007615T (pl)
WO (1) WO2021023718A1 (pl)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6183737B1 (en) * 1997-10-30 2001-02-06 The General Hospital Corporation Bonding of cartilage pieces using isolated chondrocytes and a biological gel
US20030099620A1 (en) * 1997-10-30 2003-05-29 The General Hospital Corporation Bonding of cartilaginous matrices using isolated chondrocytes
JP2004524839A (ja) * 2001-01-31 2004-08-19 インターフェース バイオテック エー／エス オートロガスな細胞の内植／移植方法のための哺乳動物の細胞のインビトロでの改良した培養方法
JP4923235B2 (ja) * 2002-12-06 2012-04-25 独立行政法人産業技術総合研究所 骨−軟骨組織の再生用移植体
US7067123B2 (en) * 2003-04-29 2006-06-27 Musculoskeletal Transplant Foundation Glue for cartilage repair
WO2005079881A1 (en) * 2004-02-19 2005-09-01 Interface Biotech A/S Arthroscopic method for cell implantation in mammals
CN102526806B (zh) * 2012-01-20 2013-12-18 陕西博鸿生物科技有限公司 一种组织工程软骨及其制备方法
US20140178343A1 (en) * 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
CN106434538A (zh) * 2016-09-29 2017-02-22 广州赛莱拉干细胞科技股份有限公司 软骨细胞的三维培养方法、制得的软骨组织与应用
CN110016461A (zh) * 2019-01-15 2019-07-16 深圳市第二人民医院 一种软骨细胞体外扩增方法

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JP2025122124A (ja) 2025-08-20
EP4007615B1 (en) 2023-09-27
HUE064647T2 (hu) 2024-03-28
CN114466923A (zh) 2022-05-10
IL290282B1 (en) 2024-10-01
EP4007615A1 (en) 2022-06-08
JP2022543174A (ja) 2022-10-07
CN114466923B (zh) 2024-06-14
WO2021023718A1 (en) 2021-02-11
DK4007615T3 (da) 2023-12-04
PT4007615T (pt) 2023-12-11
IL290282A (en) 2022-04-01
FI4007615T3 (fi) 2023-12-15
PL240714B1 (pl) 2022-05-23
IL290282B2 (en) 2025-02-01
US20220265899A1 (en) 2022-08-25
EP4007615B8 (en) 2024-01-03
KR20220042423A (ko) 2022-04-05
ES2967034T3 (es) 2024-04-25
CA3149427A1 (en) 2021-02-11

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