PL410410A1 - Sposób otrzymywania rekombinowanego białka z prekursora - Google Patents

Sposób otrzymywania rekombinowanego białka z prekursora

Info

Publication number
PL410410A1
PL410410A1 PL410410A PL41041014A PL410410A1 PL 410410 A1 PL410410 A1 PL 410410A1 PL 410410 A PL410410 A PL 410410A PL 41041014 A PL41041014 A PL 41041014A PL 410410 A1 PL410410 A1 PL 410410A1
Authority
PL
Poland
Prior art keywords
amino acid
protease
precursor
recombinant protein
obtaining recombinant
Prior art date
Application number
PL410410A
Other languages
English (en)
Other versions
PL233560B1 (pl
Inventor
Julita Balcerek
Katarzyna Sznilik
Sławomir Jaros
Maciej Wieczorek
Original Assignee
Mabion Spółka Akcyjna
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mabion Spółka Akcyjna filed Critical Mabion Spółka Akcyjna
Priority to PL41041014A priority Critical patent/PL233560B1/pl
Priority to PCT/PL2015/000197 priority patent/WO2016089234A1/en
Priority to EP15828395.2A priority patent/EP3227321A1/en
Priority to US15/532,811 priority patent/US20180105570A1/en
Publication of PL410410A1 publication Critical patent/PL410410A1/pl
Publication of PL233560B1 publication Critical patent/PL233560B1/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/17—Metallocarboxypeptidases (3.4.17)
    • C12Y304/17002—Carboxypeptidase B (3.4.17.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21061—Kexin (3.4.21.61), i.e. proprotein convertase subtilisin/kexin type 9

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Sposób otrzymywania białka rekombinowanego z prekursora, korzystnie insulin ssaczych w tym insulin ludzkich i ich analogów polega na tym, że używa się proteazy hydrolizującej jedno lub więcej wiązań peptydowych znajdujących się w tym białku, przy czym proteaza przerywa wiązanie peptydowe od strony C końca aminokwasu zasadowego, kiedy aminokwas ten występuje jako drugi w kolejności po innym zasadowym lub obojętnym aminokwasie, a kolejność ta umożliwia specyficzne rozpoznanie obydwu aminokwasów przez proteazę. Wynalazek znajduje zastosowanie w biotechnologii i przemyśle farmaceutycznym.
PL41041014A 2014-12-05 2014-12-05 Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka PL233560B1 (pl)

Priority Applications (4)

Application Number Priority Date Filing Date Title
PL41041014A PL233560B1 (pl) 2014-12-05 2014-12-05 Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
PCT/PL2015/000197 WO2016089234A1 (en) 2014-12-05 2015-12-04 Method for preparation of a recombinant protein from a precusor
EP15828395.2A EP3227321A1 (en) 2014-12-05 2015-12-04 Method for preparation of a recombinant protein from a precusor
US15/532,811 US20180105570A1 (en) 2014-12-05 2015-12-04 Method for preparation of a recombinant protein from a precusor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL41041014A PL233560B1 (pl) 2014-12-05 2014-12-05 Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka

Publications (2)

Publication Number Publication Date
PL410410A1 true PL410410A1 (pl) 2016-06-06
PL233560B1 PL233560B1 (pl) 2019-10-31

Family

ID=55221477

Family Applications (1)

Application Number Title Priority Date Filing Date
PL41041014A PL233560B1 (pl) 2014-12-05 2014-12-05 Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka

Country Status (4)

Country Link
US (1) US20180105570A1 (pl)
EP (1) EP3227321A1 (pl)
PL (1) PL233560B1 (pl)
WO (1) WO2016089234A1 (pl)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10799564B1 (en) 2019-05-06 2020-10-13 Baxter International Inc. Insulin premix formulation and product, methods of preparing same, and methods of using same

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK58285D0 (da) 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
US4929553A (en) * 1987-05-29 1990-05-29 Canadian Patents & Development Ltd. Protease for specific processing of secreted proteins
DK105489D0 (da) 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
GB9513967D0 (en) * 1995-07-08 1995-09-06 Univ Leicester Insulin
JP5366546B2 (ja) 2005-08-16 2013-12-11 ノボ・ノルデイスク・エー／エス 成熟インスリンポリペプチドの作製方法
DE102006031955A1 (de) 2006-07-11 2008-01-17 Sanofi-Aventis Deutschland Gmbh Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
US8518668B2 (en) * 2006-09-27 2013-08-27 Novo Nordisk A/S Method for making maturated insulin polypeptides in a fungal cell

Also Published As

Publication number Publication date
WO2016089234A4 (en) 2016-07-14
US20180105570A1 (en) 2018-04-19
EP3227321A1 (en) 2017-10-11
PL233560B1 (pl) 2019-10-31
WO2016089234A1 (en) 2016-06-09

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