PL402440A1 - Zmodyfikowany gen ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID), sposób modyfikacji genu ludzkiej AID, preparat wykazujący aktywność AID oraz sposób otrzymywania tego preparatu w systemie bakteryjnym - Google Patents

Zmodyfikowany gen ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID), sposób modyfikacji genu ludzkiej AID, preparat wykazujący aktywność AID oraz sposób otrzymywania tego preparatu w systemie bakteryjnym

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Publication number
PL402440A1
PL402440A1 PL402440A PL40244013A PL402440A1 PL 402440 A1 PL402440 A1 PL 402440A1 PL 402440 A PL402440 A PL 402440A PL 40244013 A PL40244013 A PL 40244013A PL 402440 A1 PL402440 A1 PL 402440A1
Authority
PL
Poland
Prior art keywords
aid
gene
human
preparation
activity
Prior art date
Application number
PL402440A
Other languages
English (en)
Other versions
PL225333B1 (pl
Inventor
Lucyna Budźko
Paulina Jackowiak
Marek Figlerowicz
Original Assignee
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Chemii Bioorganicznej Polskiej Akademii Nauk filed Critical Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Priority to PL402440A priority Critical patent/PL225333B1/pl
Priority to PCT/PL2014/000003 priority patent/WO2014109656A1/en
Priority to US14/384,665 priority patent/US20150044675A1/en
Priority to EP14703938.2A priority patent/EP2870241B1/en
Priority to DK14703938.2T priority patent/DK2870241T3/en
Publication of PL402440A1 publication Critical patent/PL402440A1/pl
Publication of PL225333B1 publication Critical patent/PL225333B1/pl

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y305/00Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005Cytidine deaminase (3.5.4.5)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Przedmiotem wynalazku jest zmodyfikowany gen ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID), sposób modyfikacji genu ludzkiej AID, preparat wykazujący aktywność AID oraz sposób wytwarzania tego preparatu. Wynalazek dotyczy zmodyfikowanego genu ludzkiej AID, umożliwiającego produkcję aktywnego enzymu w systemie bakteryjnym.
PL402440A 2013-01-14 2013-01-14 Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym PL225333B1 (pl)

Priority Applications (5)

Application Number Priority Date Filing Date Title
PL402440A PL225333B1 (pl) 2013-01-14 2013-01-14 Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym
PCT/PL2014/000003 WO2014109656A1 (en) 2013-01-14 2014-01-14 Modified gene of human activation-induced cytidine deaminase (aid), a method of modification of human aid gene, a composition showing aid activity, a method of preparation of such composition in a bacterial system and use of the composition in the analyses of dna/rna amination/deamination and/or methylation/demethylation
US14/384,665 US20150044675A1 (en) 2013-01-14 2014-01-14 Modified gene of human activation-induced cytidine deaminase (aid), a method of modification of human aid gene, a composition showing aid activity, a method of preparation of such composition in a bacterial system and use of the composition in the analyses of dna/rna amination/deamination and/or methylation/demethylation
EP14703938.2A EP2870241B1 (en) 2013-01-14 2014-01-14 A composition showing activation-induced cytidine deaminase (aid) activity, a method of preparation of such composition in a bacterial system and use of the composition in the analyses of dna/rna amination/deamination and/or methylation/demethylation
DK14703938.2T DK2870241T3 (en) 2013-01-14 2014-01-14 COMPOSITION EXISTING ACTIVITY-INDUCED CYTIDINE DAMINASE (AID) ACTIVITY, PROCEDURE FOR PROVIDING THIS COMPOSITION IN A BACTERIAL SYSTEM AND USING THE COMPOSITION / DEVELOPMENT / ANALYSIS OF ANALYZE / DEVELOPMENT / ANALYZE / ANALYZE COMPOSITION

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL402440A PL225333B1 (pl) 2013-01-14 2013-01-14 Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym

Publications (2)

Publication Number Publication Date
PL402440A1 true PL402440A1 (pl) 2014-07-21
PL225333B1 PL225333B1 (pl) 2017-03-31

Family

ID=50073407

Family Applications (1)

Application Number Title Priority Date Filing Date
PL402440A PL225333B1 (pl) 2013-01-14 2013-01-14 Preparat wykazujący aktywność ludzkiej deaminazy cytydyny indukowanej aktywacją limfocytów B (AID) oraz sposób otrzymywania tego preparatu w systemie bakteryjnym

Country Status (5)

Country Link
US (1) US20150044675A1 (pl)
EP (1) EP2870241B1 (pl)
DK (1) DK2870241T3 (pl)
PL (1) PL225333B1 (pl)
WO (1) WO2014109656A1 (pl)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2228378A1 (en) * 1995-07-31 1997-02-13 Centre De Recherche De L'hopital Sainte-Justine Cytidine deaminase cdna as a positive selectable marker for gene transfer, gene therapy and protein synthesis
EP1575992A4 (en) * 2002-08-05 2007-02-21 Univ Rochester CHIMERIC PROTEINS WITH PROTEIN TRANSDUCTION FIELD / DOMAINE DESAMINASE, ASSOCIATED COMPOUNDS AND CORRESPONDING USES
RU2537264C2 (ru) * 2009-04-03 2014-12-27 Медикал Рисерч Каунсил Мутанты индуцируемой активацией цитидиндеаминазы (aid) и способы их применения

Also Published As

Publication number Publication date
EP2870241B1 (en) 2018-03-14
PL225333B1 (pl) 2017-03-31
US20150044675A1 (en) 2015-02-12
EP2870241A1 (en) 2015-05-13
DK2870241T3 (en) 2018-06-06
WO2014109656A1 (en) 2014-07-17

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