PH12022550496A1 - Methods of generating and isolating midbrain dopamine neurons - Google Patents

Methods of generating and isolating midbrain dopamine neurons

Info

Publication number
PH12022550496A1
PH12022550496A1 PH1/2022/550496A PH12022550496A PH12022550496A1 PH 12022550496 A1 PH12022550496 A1 PH 12022550496A1 PH 12022550496 A PH12022550496 A PH 12022550496A PH 12022550496 A1 PH12022550496 A1 PH 12022550496A1
Authority
PH
Philippines
Prior art keywords
methods
isolating
generating
dopamine neurons
midbrain dopamine
Prior art date
Application number
PH1/2022/550496A
Other languages
English (en)
Inventor
Stefan Irion
Taewan Kim
So Yeon Koo
Lorenz Studer
Original Assignee
Memorial Sloan Kettering Cancer Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Memorial Sloan Kettering Cancer Center filed Critical Memorial Sloan Kettering Cancer Center
Publication of PH12022550496A1 publication Critical patent/PH12022550496A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Neurosurgery (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Ophthalmology & Optometry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Psychiatry (AREA)
  • Hospice & Palliative Care (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PH1/2022/550496A 2019-08-29 2020-08-31 Methods of generating and isolating midbrain dopamine neurons PH12022550496A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962893674P 2019-08-29 2019-08-29
PCT/US2020/048733 WO2021042027A1 (en) 2019-08-29 2020-08-31 Methods of generating and isolating midbrain dopamine neurons

Publications (1)

Publication Number Publication Date
PH12022550496A1 true PH12022550496A1 (en) 2023-03-20

Family

ID=74686069

Family Applications (1)

Application Number Title Priority Date Filing Date
PH1/2022/550496A PH12022550496A1 (en) 2019-08-29 2020-08-31 Methods of generating and isolating midbrain dopamine neurons

Country Status (13)

Country Link
US (1) US20220177835A1 (https=)
EP (1) EP4021477A4 (https=)
JP (2) JP7759870B2 (https=)
KR (1) KR20220051388A (https=)
CN (2) CN121227633A (https=)
AU (1) AU2020336221A1 (https=)
BR (1) BR112022003826A2 (https=)
CA (1) CA3152522A1 (https=)
IL (1) IL290930A (https=)
MX (1) MX2022002532A (https=)
PH (1) PH12022550496A1 (https=)
WO (1) WO2021042027A1 (https=)
ZA (1) ZA202202994B (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3792346B1 (en) 2019-09-13 2023-07-26 Miltenyi Biotec B.V. & Co. KG Method for the generation of a cell composition of ventral midbrain dopaminergic progenitor cells
AU2022247250A1 (en) 2021-03-30 2023-11-09 Trailhead Biosystems Inc. Methods and compositions for generating oligodendrocyte progenitor cells
JP2024523738A (ja) 2021-07-14 2024-06-28 ノヴォ ノルディスク アー／エス ニューロンおよびその前駆体が富化された細胞集団を提供するための方法
CN117089520A (zh) * 2021-10-28 2023-11-21 中国科学院动物研究所 一种中脑多巴胺细胞群、其制造方法以及用途
JP2025524856A (ja) * 2022-07-21 2025-08-01 トレイルヘッド バイオシステムズ インコーポレイテッド 神経前駆細胞からヒト中脳ドーパミン作動性ニューロンを作製するための方法および組成物
US12565639B2 (en) 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
WO2025166063A1 (en) 2024-01-31 2025-08-07 Bluerock Therapeutics Lp Igfbp2 in cell culture
CN117838835B (zh) * 2024-02-01 2024-11-08 暨南大学 成纤维细胞生长因子17蛋白和/或其激活剂在制备治疗阿尔兹海默病药物中的应用
WO2025252180A1 (zh) * 2024-06-05 2025-12-11 中国科学院脑科学与智能技术卓越创新中心 中脑多巴胺能祖细胞群的体外制备方法
CN119776284B (zh) * 2025-03-13 2025-06-20 首都医科大学宣武医院 含有多巴胺能荧光报告系统的细胞及异步共培养方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7632679B2 (en) * 2002-07-16 2009-12-15 The Trustees Of Columbia University In The City Of New York Systems and methods for screening for modulators of neural differentiation
WO2010096496A2 (en) * 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
JP6090937B2 (ja) * 2011-07-27 2017-03-08 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞マーカー
EP3693455A1 (en) * 2011-11-04 2020-08-12 Memorial Sloan-Kettering Cancer Center Midbrain dopamine (da) neurons for engraftment
EP2614829A1 (en) * 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
AU2014316100B2 (en) * 2013-09-05 2020-11-05 Eisai R&D Management Co., Ltd. New method for inducing dopamine-producing neural precursor cells
JP6998772B2 (ja) * 2015-04-09 2022-02-04 ビオラミナ アーベー 神経変性疾患の処置における使用のための幹細胞由来のドパミン作用性細胞を生成するための方法および組成物
AU2016270793B2 (en) * 2015-06-01 2022-06-09 Memorial Sloan-Kettering Cancer Center Methods of in vitro differentiation of midbrain dopamine (MDA) neurons
KR102625865B1 (ko) * 2016-08-16 2024-01-16 후지필름 셀룰러 다이내믹스, 인코포레이티드 만능 세포를 분화시키는 방법

Also Published As

Publication number Publication date
EP4021477A1 (en) 2022-07-06
CN114650836B (zh) 2025-10-17
CN121227633A (zh) 2025-12-30
BR112022003826A2 (pt) 2022-05-31
JP2022546482A (ja) 2022-11-04
ZA202202994B (en) 2022-12-21
US20220177835A1 (en) 2022-06-09
JP2026001236A (ja) 2026-01-06
IL290930A (en) 2022-04-01
KR20220051388A (ko) 2022-04-26
MX2022002532A (es) 2022-06-16
CN114650836A (zh) 2022-06-21
AU2020336221A1 (en) 2022-04-07
WO2021042027A1 (en) 2021-03-04
JP7759870B2 (ja) 2025-10-24
EP4021477A4 (en) 2023-12-13
CA3152522A1 (en) 2021-03-04

Similar Documents

Publication Publication Date Title
PH12022550496A1 (en) Methods of generating and isolating midbrain dopamine neurons
TN2023000250A1 (en) Inhibiting ubiquitin-specific protease 1 (usp1)
PH12022550605A1 (en) Hpk1 antagonists and uses thereof
WO2021071788A3 (en) Oligonucleotide compositions and methods of use thereof
WO2020227691A3 (en) Oligonucleotide compositions and methods of use thereof
WO2020243415A3 (en) Tead inhibitors and uses thereof
PH12021552759A1 (en) Substituted cycloalkyls as modulators of the integrated stress pathway
PH12021500013A1 (en) New heterocyclic compounds as monoacylgylcerol lipase inhibitors
WO2020176597A9 (en) N-substituted indoles and other heterocycles for treating brain disorders
ZA202006006B (en) Arginase inhibitors
PH12021500008A1 (en) Transglutaminase 2 (tg2) inhibitors
WO2020252229A3 (en) Inhibitors of sarm1
MX2021003819A (es) Moduladores de alfa-1 antitripsina.
CR20210056A (es) Nuevos compuestos heterocíclicos como inhibidores de monoacilglicerol lipasa
PH12023552079A1 (en) Cdk2 inhibitors and methods of using the same
MX2021006831A (es) Inhibidores de alk5.
PH12020552212A1 (en) Compositions and methods for the treatment of hemoglobinopathies and thalassemias
MX2020006290A (es) Dinucleotidos ciclicos como agonistas de sting.
PH12022551623A1 (en) Substituted pyrazolo-pyrimidines and uses thereof
MX2024001243A (es) Composiciones y metodos para el tratamiento de melanoma.
ZA202309516B (en) Anti-ccr8 antibodies
PH12022550491A1 (en) Perk inhibiting pyrrolopyrimidine compounds
WO2023086428A3 (en) Novel modulators of the aryl hydrocarbon receptor and methods of use thereof
CR20220004A (es) Nuevos compuestos heterocíclicos
WO2022232016A3 (en) Chimeric receptors targeting adgre2 and/or clec12a and uses thereof