JP7759870B2 - 中脳ドーパミンニューロンの生成及び単離方法 - Google Patents

中脳ドーパミンニューロンの生成及び単離方法

Info

Publication number
JP7759870B2
JP7759870B2 JP2022513499A JP2022513499A JP7759870B2 JP 7759870 B2 JP7759870 B2 JP 7759870B2 JP 2022513499 A JP2022513499 A JP 2022513499A JP 2022513499 A JP2022513499 A JP 2022513499A JP 7759870 B2 JP7759870 B2 JP 7759870B2
Authority
JP
Japan
Prior art keywords
cells
days
activator
certain embodiments
signaling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022513499A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022546482A (ja
JP2022546482A5 (https=
Inventor
ローレンツ ステューダー，
テワン キム，
ステファン イリオン，
ソ ヨン ク，
Original Assignee
メモリアル スローン ケタリング キャンサー センター
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by メモリアル スローン ケタリング キャンサー センター filed Critical メモリアル スローン ケタリング キャンサー センター
Publication of JP2022546482A publication Critical patent/JP2022546482A/ja
Publication of JP2022546482A5 publication Critical patent/JP2022546482A5/ja
Priority to JP2025172808A priority Critical patent/JP2026001236A/ja
Application granted granted Critical
Publication of JP7759870B2 publication Critical patent/JP7759870B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/03—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from non-embryonic pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Neurology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Neurosurgery (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Ophthalmology & Optometry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Psychiatry (AREA)
  • Hospice & Palliative Care (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2022513499A 2019-08-29 2020-08-31 中脳ドーパミンニューロンの生成及び単離方法 Active JP7759870B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025172808A JP2026001236A (ja) 2019-08-29 2025-10-14 中脳ドーパミンニューロンの生成及び単離方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962893674P 2019-08-29 2019-08-29
US62/893,674 2019-08-29
PCT/US2020/048733 WO2021042027A1 (en) 2019-08-29 2020-08-31 Methods of generating and isolating midbrain dopamine neurons

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025172808A Division JP2026001236A (ja) 2019-08-29 2025-10-14 中脳ドーパミンニューロンの生成及び単離方法

Publications (3)

Publication Number Publication Date
JP2022546482A JP2022546482A (ja) 2022-11-04
JP2022546482A5 JP2022546482A5 (https=) 2023-09-08
JP7759870B2 true JP7759870B2 (ja) 2025-10-24

Family

ID=74686069

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022513499A Active JP7759870B2 (ja) 2019-08-29 2020-08-31 中脳ドーパミンニューロンの生成及び単離方法
JP2025172808A Pending JP2026001236A (ja) 2019-08-29 2025-10-14 中脳ドーパミンニューロンの生成及び単離方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025172808A Pending JP2026001236A (ja) 2019-08-29 2025-10-14 中脳ドーパミンニューロンの生成及び単離方法

Country Status (13)

Country Link
US (1) US20220177835A1 (https=)
EP (1) EP4021477A4 (https=)
JP (2) JP7759870B2 (https=)
KR (1) KR20220051388A (https=)
CN (2) CN121227633A (https=)
AU (1) AU2020336221A1 (https=)
BR (1) BR112022003826A2 (https=)
CA (1) CA3152522A1 (https=)
IL (1) IL290930A (https=)
MX (1) MX2022002532A (https=)
PH (1) PH12022550496A1 (https=)
WO (1) WO2021042027A1 (https=)
ZA (1) ZA202202994B (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3792346B1 (en) 2019-09-13 2023-07-26 Miltenyi Biotec B.V. & Co. KG Method for the generation of a cell composition of ventral midbrain dopaminergic progenitor cells
AU2022247250A1 (en) 2021-03-30 2023-11-09 Trailhead Biosystems Inc. Methods and compositions for generating oligodendrocyte progenitor cells
JP2024523738A (ja) 2021-07-14 2024-06-28 ノヴォ ノルディスク アー／エス ニューロンおよびその前駆体が富化された細胞集団を提供するための方法
CN117089520A (zh) * 2021-10-28 2023-11-21 中国科学院动物研究所 一种中脑多巴胺细胞群、其制造方法以及用途
JP2025524856A (ja) * 2022-07-21 2025-08-01 トレイルヘッド バイオシステムズ インコーポレイテッド 神経前駆細胞からヒト中脳ドーパミン作動性ニューロンを作製するための方法および組成物
US12565639B2 (en) 2023-06-13 2026-03-03 Aspen Neuroscience, Inc. Methods for differentiating dopaminergic neurons from stem cells
WO2025166063A1 (en) 2024-01-31 2025-08-07 Bluerock Therapeutics Lp Igfbp2 in cell culture
CN117838835B (zh) * 2024-02-01 2024-11-08 暨南大学 成纤维细胞生长因子17蛋白和/或其激活剂在制备治疗阿尔兹海默病药物中的应用
WO2025252180A1 (zh) * 2024-06-05 2025-12-11 中国科学院脑科学与智能技术卓越创新中心 中脑多巴胺能祖细胞群的体外制备方法
CN119776284B (zh) * 2025-03-13 2025-06-20 首都医科大学宣武医院 含有多巴胺能荧光报告系统的细胞及异步共培养方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014532440A (ja) 2011-11-04 2014-12-08 メモリアル スローン−ケタリング キャンサー センター 移植用中脳ドーパミン（ｄａ）ニューロン
WO2015034012A1 (ja) 2013-09-05 2015-03-12 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞の誘導方法
WO2018035214A1 (en) 2016-08-16 2018-02-22 Cellular Dynamics International., Inc. Methods for differentiating pluripotent cells
JP2018522538A (ja) 2015-06-01 2018-08-16 メモリアル スローン ケタリング キャンサー センター 中脳ドーパミン（ｍＤＡ）ニューロンのｉｎ ｖｉｔｒｏ分化の方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7632679B2 (en) * 2002-07-16 2009-12-15 The Trustees Of Columbia University In The City Of New York Systems and methods for screening for modulators of neural differentiation
WO2010096496A2 (en) * 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
JP6090937B2 (ja) * 2011-07-27 2017-03-08 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞マーカー
EP2614829A1 (en) * 2012-01-11 2013-07-17 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Mammalian neural plate border stem cells capable of forming neural tube and neural crest cell lineages including central and peripheral neurons
JP6998772B2 (ja) * 2015-04-09 2022-02-04 ビオラミナ アーベー 神経変性疾患の処置における使用のための幹細胞由来のドパミン作用性細胞を生成するための方法および組成物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014532440A (ja) 2011-11-04 2014-12-08 メモリアル スローン−ケタリング キャンサー センター 移植用中脳ドーパミン（ｄａ）ニューロン
WO2015034012A1 (ja) 2013-09-05 2015-03-12 国立大学法人京都大学 新規ドーパミン産生神経前駆細胞の誘導方法
JP2018522538A (ja) 2015-06-01 2018-08-16 メモリアル スローン ケタリング キャンサー センター 中脳ドーパミン（ｍＤＡ）ニューロンのｉｎ ｖｉｔｒｏ分化の方法
WO2018035214A1 (en) 2016-08-16 2018-02-22 Cellular Dynamics International., Inc. Methods for differentiating pluripotent cells

Also Published As

Publication number Publication date
EP4021477A1 (en) 2022-07-06
CN114650836B (zh) 2025-10-17
CN121227633A (zh) 2025-12-30
BR112022003826A2 (pt) 2022-05-31
JP2022546482A (ja) 2022-11-04
ZA202202994B (en) 2022-12-21
US20220177835A1 (en) 2022-06-09
JP2026001236A (ja) 2026-01-06
PH12022550496A1 (en) 2023-03-20
IL290930A (en) 2022-04-01
KR20220051388A (ko) 2022-04-26
MX2022002532A (es) 2022-06-16
CN114650836A (zh) 2022-06-21
AU2020336221A1 (en) 2022-04-07
WO2021042027A1 (en) 2021-03-04
EP4021477A4 (en) 2023-12-13
CA3152522A1 (en) 2021-03-04

Similar Documents

Publication Publication Date Title
JP7759870B2 (ja) 中脳ドーパミンニューロンの生成及び単離方法
JP7737787B2 (ja) 中脳ドーパミン（ｍＤＡ）ニューロンのｉｎ ｖｉｔｒｏ分化の方法
US20230143486A1 (en) Methods of generating midbrain dopamine neurons, midbrain neurons and uses thereof
RU2856353C1 (ru) Способы генерации и выделения дофаминовых нейронов среднего мозга
HK40075940A (zh) 中脑多巴胺神经元的产生和分离方法
CA2987617C (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons
WO2026039433A1 (en) Midbrain dopaminergic neuron compositions and methods of use
HK40081864A (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons
HK40082714A (en) Methods of in vitro differentiation of midbrain dopamine (mda) neurons

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230831

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230831

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20241101

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250203

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250522

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250820

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250912

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20251014

R150 Certificate of patent or registration of utility model

Ref document number: 7759870

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150