PE20191838A1 - Clarificacion de cultivos celulares - Google Patents

Clarificacion de cultivos celulares

Info

Publication number
PE20191838A1
PE20191838A1 PE2019001970A PE2019001970A PE20191838A1 PE 20191838 A1 PE20191838 A1 PE 20191838A1 PE 2019001970 A PE2019001970 A PE 2019001970A PE 2019001970 A PE2019001970 A PE 2019001970A PE 20191838 A1 PE20191838 A1 PE 20191838A1
Authority
PE
Peru
Prior art keywords
cell culture
solution
clarification
harvested
cell
Prior art date
Application number
PE2019001970A
Other languages
English (en)
Inventor
Jose Castillo
Vasily Medvedev
Original Assignee
Univercells S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univercells S A filed Critical Univercells S A
Publication of PE20191838A1 publication Critical patent/PE20191838A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D37/00—Processes of filtration
    • B01D37/02—Precoating the filter medium; Addition of filter aids to the liquid being filtered
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/10—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising silica or silicate
    • B01J20/14—Diatomaceous earth
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2239/00—Aspects relating to filtering material for liquid or gaseous fluids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Geochemistry & Mineralogy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se describe un metodo y un kit para la clarificacion de cultivos celulares, asi como un sistema o aparato para clarificar un cultivo celular y purificar biomoleculas de interes. El cultivo celular comprende por lo menos una biomolecula de interes. El metodo comprende agregar uno o mas compuestos al cultivo celular que permite la formacion de floculos de solutos y/o particulados en dicho material cosechado del cultivo celular, agregar una cantidad de tierra de diatomeas (DE) a dicho material cosechado del cultivo celular, agitar la solucion obtenida, y transferir dicha solucion a un recipiente de filtracion que comprende un filtro soporte que tiene una superficie, permitiendo que la tierra de diatomeas en dicha solucion formen una capa en dicha superficie y filtrar dicha solucion del material cosechado a traves de dicha capa y filtro soporte
PE2019001970A 2017-03-30 2018-03-30 Clarificacion de cultivos celulares PE20191838A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE2017/5210A BE1025089B1 (fr) 2017-03-30 2017-03-30 Clarification de culture cellulaire
PCT/EP2018/058366 WO2018178376A1 (en) 2017-03-30 2018-03-30 Cell culture clarification

Publications (1)

Publication Number Publication Date
PE20191838A1 true PE20191838A1 (es) 2019-12-31

Family

ID=59269718

Family Applications (1)

Application Number Title Priority Date Filing Date
PE2019001970A PE20191838A1 (es) 2017-03-30 2018-03-30 Clarificacion de cultivos celulares

Country Status (10)

Country Link
US (1) US20200056144A1 (es)
EP (1) EP3601585A1 (es)
KR (1) KR20190135489A (es)
BE (1) BE1025089B1 (es)
CA (1) CA3058700A1 (es)
CO (1) CO2019011204A2 (es)
EC (1) ECSP19073391A (es)
MX (1) MX2019011617A (es)
PE (1) PE20191838A1 (es)
WO (1) WO2018178376A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109311937A (zh) * 2016-06-15 2019-02-05 新加坡科技研究局 增强用于纯化蛋白质的色谱法的性能的方法
WO2019215344A2 (en) * 2018-05-11 2019-11-14 Univercells Nv System and method for clarifying a cell culture harvest solution
WO2020079274A1 (en) 2018-10-19 2020-04-23 Univercells S.A. Method for decontaminating a biomolecule production system and a system suitable for decontamination
US20220081666A1 (en) * 2018-12-21 2022-03-17 Ichnos Sciences SA Methods of cell culture clarification
CN110194784A (zh) * 2019-05-07 2019-09-03 上海药明生物技术有限公司 运用硅藻土澄清细胞发酵液的方法
JPWO2021172203A1 (es) * 2020-02-28 2021-09-02
WO2022128875A1 (en) * 2020-12-14 2022-06-23 Chreto Aps A method and a system for filtering a suspension
US11319520B1 (en) * 2021-09-21 2022-05-03 Upside Foods, Inc. Filter cake-based systems and methods for the cultivation of cells and cell biomass

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878938A (en) * 1957-04-02 1959-03-24 Dee John Chandler Swimming pool filter
NZ228374A (en) * 1988-03-21 1990-12-21 Du Pont Method for separating and detecting microorganisms from a difficult-to-separate fluid sample, and apparatus therefor
US20070244305A1 (en) * 2004-01-30 2007-10-18 Suomen Punainen Risti Veripalvelu Process for The Manufacture of Virus Safe Immunoglobulin
US9243207B2 (en) * 2012-02-29 2016-01-26 Exxonmobil Research And Engineering Company Solvent extraction of products from algae
EP2687592A1 (de) * 2012-07-20 2014-01-22 greenovation Biotech GmbH Filtration von Zellkulturüberständen
KR20150118103A (ko) * 2013-02-06 2015-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 단백질 정제 방법
WO2015130222A1 (en) * 2014-02-27 2015-09-03 Agency For Science, Technology And Research Antibody purification process
CN109311937A (zh) * 2016-06-15 2019-02-05 新加坡科技研究局 增强用于纯化蛋白质的色谱法的性能的方法
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors

Also Published As

Publication number Publication date
BE1025089B1 (fr) 2018-10-29
US20200056144A1 (en) 2020-02-20
KR20190135489A (ko) 2019-12-06
EP3601585A1 (en) 2020-02-05
WO2018178376A1 (en) 2018-10-04
BE1025089A1 (fr) 2018-10-23
ECSP19073391A (es) 2019-10-31
MX2019011617A (es) 2019-11-18
CA3058700A1 (en) 2018-10-04
CO2019011204A2 (es) 2020-01-17

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