PE15097A1 - Acumulacion intensificada de trehalosa en las plantas - Google Patents

Acumulacion intensificada de trehalosa en las plantas

Info

Publication number
PE15097A1
PE15097A1 PE1996000004A PE00000496A PE15097A1 PE 15097 A1 PE15097 A1 PE 15097A1 PE 1996000004 A PE1996000004 A PE 1996000004A PE 00000496 A PE00000496 A PE 00000496A PE 15097 A1 PE15097 A1 PE 15097A1
Authority
PE
Peru
Prior art keywords
trehalose
gene
trehalase
inhibitor
yauin
Prior art date
Application number
PE1996000004A
Other languages
English (en)
Inventor
Oscar Johannes Maria Goddijn
Teunis Cornelis Verwoerd
Ronny Wilhelmus Hermanus Henrika Krutwagen
Eline Voogd
Original Assignee
Syngenta Mogen Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Mogen Bv filed Critical Syngenta Mogen Bv
Publication of PE15097A1 publication Critical patent/PE15097A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01028—Alpha,alpha-trehalase (3.2.1.28)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE REFIERE AL PROCEDIMIENTO DE OBTENCION DE TREHALOSA EN PLANTAS CON CAPACIDAD NATURAL PARA PRODUCIR TREHALOSA Y TREHALASA, QUE COMPRENDE: LA ACUMULACION DE TREHALOSA EN MAS DE 0.01%(PESO FRESCO), CULTIVADAS EN UNA SOLUCION ACUOSA DE VALIDAMICINA A 100nM Y 10 nM, PREFERIBLEMENTE 0.1nM A 1nM, EN PRESENCIA DE UN INHIBIDOR DE TREHALASA (OBTENIDO POR ALTERACION DE LA INFORMACION GENETICA), DONDE EL INHIBIDOR ES EL GEN QUE CODIFICA EN SENTIDO OPUESTO AL GEN PARA TREHALASA (ENCAJA EXACTAMENTE CON EL GEN ENDOGENO DE LA TREHALASA Y LO INHIBE) O ES LA PROTEINA 86KD DE LA CUCARACHA PERIPLANETA AMERICANA; DONDE EL GEN EXPRESIBLE INCLUYE LA REGION INICIADORA DE TRANSCRIPCION DEL GEN PATATIN, UN INDICE 5` SIN TRADUCIR, UN MARCO DE LECTURA ABIERTA CODIFICANDO LA SINTASA DE FOSFATO DE TREHALOSA DE LA E.COLI Y ABAJO DEL MARCO DE LECTURA UNA REGION DEL TERMINADOR TRANSCRIPCIONAL DEL GEN INHIBIDOR II DE LA PROTEINASA DE S.TUBEROSUM. Y LA EXTRACCION DE TREHALOSA DE LA PLANTA DE LA ESPECIE SOLANACEAE, PREFERIBLEMENTE SOLANUM TUBEROSUM O NICOTIANA TABACUM, PARA AGREGARSE A LOS ALIMENTOS ANTES DEL SECADO, COMO METODO DE CONSERVACION QUE MANTENGA SU VALOR NUTRITIVO, SABOR, OLOR, ENTRE OTROS; ADEMAS LA TREHALOSA EN LA PLANTA YA NO ES TOXICA, SINO QUE LE PERMITE TOLERANCIA A LA TENSION, AL FRIO, A LA SEQUIA, ENTRE OTROS
PE1996000004A 1995-01-04 1996-01-03 Acumulacion intensificada de trehalosa en las plantas PE15097A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95200008 1995-01-04
EP95202415 1995-09-07

Publications (1)

Publication Number Publication Date
PE15097A1 true PE15097A1 (es) 1997-06-06

Family

ID=26139012

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1996000004A PE15097A1 (es) 1995-01-04 1996-01-03 Acumulacion intensificada de trehalosa en las plantas

Country Status (8)

Country Link
AR (1) AR001337A1 (es)
AU (1) AU4437696A (es)
CU (1) CU22564A3 (es)
IL (1) IL116564A0 (es)
MA (1) MA23769A1 (es)
PE (1) PE15097A1 (es)
TN (1) TNSN96001A1 (es)
WO (1) WO1996021030A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU754482B2 (en) * 1996-01-12 2002-11-14 Syngenta Mogen Bv Enhanced accumulation of trehalose in plants
EP0784095A3 (en) * 1996-01-12 1997-12-29 Mogen International N.V. Enhanced accummulation of trehalose in plants
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
MX205414B (es) * 1996-05-08 2001-12-07 Univ Mexico Nacional Autonoma Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
EP0868916A3 (en) * 1997-03-04 2004-09-15 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Reduction inhibitory agent for active-oxygen eliminating activity
BR9809364A (pt) * 1997-05-02 2001-09-11 Mogen Int Metabolismo de regulação por modificação do nìvel de trehalose-6-fosfato por inibição dos nìveis de trehalase endógena
EP1002867A1 (en) * 1998-10-15 2000-05-24 K.U. Leuven Research & Development Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
AU2009204313B2 (en) 2008-01-03 2014-07-17 Proterro, Inc. Transgenic photosynthetic microorganisms and photobioreactor
BRPI1007973A2 (pt) * 2009-02-09 2015-09-01 Vib Vzw Uso de um gene de trealase recombinante, e uso de sobreexpressão de recombinante de trealase.
ES2461896B1 (es) * 2010-12-21 2015-03-18 Centro De Investigación Y De Estudios Avanzados Del Instituto Politécnico Nacional Métodos para obtener plantas resistentes a sequía
US8455436B2 (en) 2010-12-28 2013-06-04 Depuy Mitek, Llc Compositions and methods for treating joints
US8398611B2 (en) 2010-12-28 2013-03-19 Depuy Mitek, Inc. Compositions and methods for treating joints
US8623839B2 (en) * 2011-06-30 2014-01-07 Depuy Mitek, Llc Compositions and methods for stabilized polysaccharide formulations
US9682099B2 (en) 2015-01-20 2017-06-20 DePuy Synthes Products, Inc. Compositions and methods for treating joints

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8811115D0 (en) * 1988-05-11 1988-06-15 Ici Plc Tomatoes
ATE133198T1 (de) * 1990-03-28 1996-02-15 Gist Brocades Nv Neue hefestämme mit erhöhtem trehalosegehalt, verfahren zur gewinnung solcher hefen und verwendung dieser hefen
US5422254A (en) * 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
FI943133A0 (fi) * 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
EP0577915A1 (fr) * 1992-07-09 1994-01-12 N.V. Algist-Bruggeman Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré
UA39958C2 (uk) * 1993-06-30 2001-07-16 Моген Інтернешнл Н.В. Нуклеїнова кислота, яка при експресії в рослині або в рослинній клітині призводить до збільшення вмісту трегалози, спосіб одержання рослини з підвищеною здатністю до продукування трегалози, рекомбінантна геномна днк рослини, штам e.coli, спосіб збереження рослини, спосіб одержання трегалози.
AU4953393A (en) * 1993-08-24 1995-03-21 Mogen International N.V. Production of trehalose in plants

Also Published As

Publication number Publication date
AR001337A1 (es) 1997-10-22
WO1996021030A1 (en) 1996-07-11
AU4437696A (en) 1996-07-24
MA23769A1 (fr) 1996-10-01
IL116564A0 (en) 1996-03-31
TNSN96001A1 (fr) 1998-12-31
CU22564A3 (es) 1999-08-03

Similar Documents

Publication Publication Date Title
van Doorn et al. Senescence and programmed cell death: substance or semantics?
PE15097A1 (es) Acumulacion intensificada de trehalosa en las plantas
Çelik et al. Enzymatic and non-enzymatic comparison of two different industrial tomato (Solanum lycopersicum) varieties against drought stress
Xia et al. Induction of systemic stress tolerance by brassinosteroid in Cucumis sativus
Libik et al. Differences in the activities of some antioxidant enzymes and in H 2 O 2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant
Ferreira et al. Using LED lighting in somatic embryogenesis and micropropagation of an elite sugarcane variety and its effect on redox metabolism during acclimatization
Erland et al. Melatonin in plant signalling and behaviour
Sivanesan et al. Silicon promotes adventitious shoot regeneration and enhances salinity tolerance of Ajuga multiflora Bunge by altering activity of antioxidant enzyme
AR082240A2 (es) Metodo para obtener plantas con caracteristicas de crecimiento modificadas, una construccion; una celula huesped; una planta transgenica y usos
Hesami et al. An efficient in vitro shoot regeneration through direct organogenesis from seedling-derived petiole and leaf segments and acclimatization of Ficus religiosa
Rahmani et al. Effects of salicylic acid applied pre-or post-transport on post-harvest characteristics and antioxidant enzyme activity of gladiolus cut flower spikes
Ranwala et al. Preventive mechanisms of gibberellin4+ 7 and light on low-temperature-induced leaf senescence in Lilium cv. Stargazer
Al-Mayahi Effect of calcium and boron on growth and development of callus and shoot regeneration of date palm ‘Barhee’
Davoudi Pahnekolayi et al. Optimizing culture medium ingredients and micrografting devices can promote in vitro micrografting of cut roses on different rootstocks
De Souza et al. Morpho-anatomical changes and antioxidant enzyme activity during the acclimatization of Genipa americana
Dehestani-Ardakani et al. Salicylic acid and herbal extracts prolong vase life and improve quality of carnation (Dianthus caryophyllus L.) flowers
Singh et al. Multivariate screening approach indicated adaptive tolerance to salt stress in the seedlings of an agroforestry tree, Eucalyptus tereticornis Sm.
Al-Quraan et al. The influence of laser beam and high light intensity on lentil (Lens culinaris) and wheat (Triticum aestivum) seedlings growth and metabolism
Ozden et al. Effects of cytokinin on callus proliferation associated with physiological and biochemical changes in Vitis vinifera L.
Neamah et al. Positive response of Hyoscyamus pusillus callus cultures to exogenous melatonin on biochemical traits and secondary metabolites under drought conditions
Cortés-Antiquera et al. Heat shock tolerance in Deschampsia antarctica Desv. cultivated in vitro is mediated by enzymatic and non-enzymatic antioxidants
Labrousse et al. Nemesia root hair response to paper pulp substrate for micropropagation
Çakir et al. Selenium induced selenocysteine methyltransferase gene expression and antioxidant enzyme activities in Astragalus chrysochlorus
Wei et al. Effects of sub-optimal temperatures and low light intensity on growth and anti-oxidant enzyme activities in watermelon (Citrullus lanatus) seedlings
Ruiz-Sáenz et al. Induction of tolerance to cryogenic protocols in Solanum tuberosum by salicylic acid is mediated by enzymatic antioxidant activity and hydrogen peroxide

Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed