NZ581493A - Gene knockout mesophilic and thermophilic organisms, and methods of use thereof - Google Patents
Gene knockout mesophilic and thermophilic organisms, and methods of use thereofInfo
- Publication number
- NZ581493A NZ581493A NZ581493A NZ58149308A NZ581493A NZ 581493 A NZ581493 A NZ 581493A NZ 581493 A NZ581493 A NZ 581493A NZ 58149308 A NZ58149308 A NZ 58149308A NZ 581493 A NZ581493 A NZ 581493A
- Authority
- NZ
- New Zealand
- Prior art keywords
- microorganism
- genetically modified
- native
- thermophilic
- mesophilic
- Prior art date
Links
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ599526A NZ599526A (en) | 2007-05-09 | 2012-04-20 | Gene knockout mesophilic and gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
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| US91697807P | 2007-05-09 | 2007-05-09 | |
| PCT/US2008/063237 WO2008141174A2 (en) | 2007-05-09 | 2008-05-09 | Gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
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| NZ581493A NZ581493A (en) | 2007-05-09 | 2008-05-09 | Gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
| NZ599526A NZ599526A (en) | 2007-05-09 | 2012-04-20 | Gene knockout mesophilic and gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
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Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9650642B2 (en) * | 2008-02-08 | 2017-05-16 | Algenol Biotech LLC | Genetically modified cyanobacteria for the production of ethanol |
| WO2009109630A1 (en) * | 2008-03-07 | 2009-09-11 | Dsm Ip Assets B.V. | A pentose sugar fermenting cell |
| DE102008031350B4 (de) * | 2008-07-02 | 2011-02-10 | Johann Wolfgang Goethe-Universität Frankfurt am Main | Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen |
| EP2313513A4 (en) | 2008-07-28 | 2012-01-11 | Univ Massachusetts | METHOD AND COMPOSITION FOR IMPROVED MANUFACTURE OF PRODUCTS IN MICROORGANISMS |
| CA2743505A1 (en) * | 2008-11-12 | 2010-05-20 | Mascoma Corporation | Gene knockout mesophilic and thermophilic organisms, and methods of use thereof |
| EP2194120A1 (en) * | 2008-12-02 | 2010-06-09 | Total S.A. | Bioprocessing ligno-cellulose into ethanol with recombinant clostridium |
| WO2010075213A2 (en) * | 2008-12-22 | 2010-07-01 | Mascoma Corporation | Production of ethanol from lignocellulosic biomass |
| WO2011059314A1 (en) * | 2009-11-12 | 2011-05-19 | Stichting Voor De Technische Wetenschappen | Pentose transporters and uses thereof |
| EP2529003A2 (en) | 2010-01-26 | 2012-12-05 | Scale Biofuel APS | Methods for producing and harvesting ethanol and apparatus for producing and harvesting the same |
| GB2478791A (en) * | 2010-03-19 | 2011-09-21 | Qteros Inc | Ethanol production by genetically-modified bacteria |
| US8927254B2 (en) | 2010-09-29 | 2015-01-06 | University Of Georgia Research Foundation, Inc. | Pyrococcus furiosus strains and methods of using same |
| US9546385B2 (en) | 2010-12-22 | 2017-01-17 | Enchi Corporation | Genetically modified clostridium thermocellum engineered to ferment xylose |
| AU2012223494B2 (en) | 2011-02-28 | 2017-04-06 | Dsm Nutritional Products, Llc | Polymeric acid catalysts and uses thereof |
| US8962333B2 (en) | 2011-04-04 | 2015-02-24 | University Of Georgia Research Foundation, Inc. | Restriction/modification polypeptides, polynucleotides, and methods |
| AP3983A (en) | 2011-05-18 | 2017-01-06 | Scale Biofuel Aps | Solar-assisted volatile fermentation products production processes |
| WO2013041668A1 (en) * | 2011-09-22 | 2013-03-28 | Direvo Industrial Biotechnology Gmbh | Single-step bioconversion of lignocellulosic biomass to biofuels using extreme thermophilic bacteria |
| CA2850480A1 (en) | 2011-09-30 | 2013-06-20 | Mascoma Corporation | Engineering microorganisms to increase ethanol production by metabolic redirection |
| CA2857498A1 (en) * | 2011-11-30 | 2013-09-26 | Mascoma Corporation | Engineering an increase in ethanol production by altering cofactor specificity |
| US9309542B2 (en) | 2012-08-17 | 2016-04-12 | University Of Georgia Research Foundation, Inc. | Recombinant Caldicellulosiruptor bescii and methods of use |
| US9238845B2 (en) | 2012-08-24 | 2016-01-19 | Midori Usa, Inc. | Methods of producing sugars from biomass feedstocks |
| US9587256B2 (en) | 2012-09-06 | 2017-03-07 | University Of Georgia Research Foundation, Inc. | Sequestration of carbon dioxide with hydrogen to useful products |
| US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
| US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
| MY203868A (en) | 2014-06-11 | 2024-07-22 | Univ Duke | Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves |
| CN104263680B (zh) * | 2014-09-05 | 2017-02-01 | 华南理工大学 | 一种嗜热厌氧杆菌及利用其生产乙醇的方法 |
| CN105154465A (zh) * | 2015-07-23 | 2015-12-16 | 昆明理工大学 | 一种敲除乙酸激酶基因的重组质粒及其应用 |
| CN106893745A (zh) * | 2017-04-12 | 2017-06-27 | 浙江畯和生物科技有限公司 | 一种木质纤维素生物质的高效转化利用方法 |
| TWI664286B (zh) * | 2017-08-02 | 2019-07-01 | 國立清華大學 | 生產醋酸的基因轉殖藍綠菌及其應用 |
| WO2019246488A1 (en) | 2018-06-21 | 2019-12-26 | Duke University | Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control |
| US12275962B2 (en) | 2019-07-02 | 2025-04-15 | Codexis, Inc. | Engineered acetate kinase variant enzymes |
| CN110484466B (zh) * | 2019-08-15 | 2023-04-21 | 华南理工大学 | 一种提高嗜热厌氧杆菌发酵性能的方法 |
| CN111423256A (zh) * | 2020-04-23 | 2020-07-17 | 上海国璨环境科技有限公司 | 一种有机垃圾热解方法 |
Family Cites Families (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027895A (en) | 1995-09-12 | 2000-02-22 | The Ohio State University Research Foundation | Methods for cleaving DNA with nucleotide integrases |
| US5698421A (en) | 1995-09-12 | 1997-12-16 | The Ohio State Research Foundation | Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site |
| DK0853127T3 (da) * | 1996-09-27 | 2005-03-07 | Dsm Ip Assets Bv | Biotin-biosyntese-gener II |
| US5804418A (en) | 1996-11-19 | 1998-09-08 | The Ohio State University Research Foundation | Methods for preparing nucleotide integrases |
| WO1999043854A1 (en) | 1998-02-26 | 1999-09-02 | The Ohio State Research Foundation | Methods for cleaving single-stranded and double-stranded dna substrates with nucleotide integrase |
| GB0000185D0 (en) | 2000-01-06 | 2000-03-01 | Agrol Limited | Ethanol production |
| GB0011186D0 (en) | 2000-05-09 | 2000-06-28 | Agrol Limited | Modification of bacteria |
| CN1336432A (zh) * | 2000-07-31 | 2002-02-20 | 武汉迪普生物技术有限公司 | 重组枯激酶的生产方法及其生物活性 |
| CA2424890C (en) | 2000-10-06 | 2014-06-03 | Elsworth Biotechnology Limited | Ethanol production in gram-positive bacteria with a stabilized mutation in lactate dehydrogenase |
| US20030165859A1 (en) | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| US7026464B2 (en) * | 2002-10-21 | 2006-04-11 | E. I. Du Pont De Nemours And Company | Natural promoters for gene expression in C1 metabolizing bacteria |
| RU2346996C2 (ru) | 2004-06-29 | 2009-02-20 | ЮРОПИЭН НИКЕЛЬ ПиЭлСи | Усовершенствованное выщелачивание основных металлов |
| US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
| WO2006099615A2 (en) * | 2005-03-16 | 2006-09-21 | The Johns Hopkins University | Adenoviral fiber exchange shuttle system |
| JP2008539710A (ja) | 2005-05-04 | 2008-11-20 | ティーエムオー リニューアブルズ リミティド | エタノール生産用の不活性化乳酸デヒドロゲナーゼ（ｌｄｈ）遺伝子を有する好熱性微生物 |
| GB0511602D0 (en) | 2005-06-07 | 2005-07-13 | Tmo Biotec Ltd | Microorganisms |
| WO2007000020A1 (en) | 2005-06-29 | 2007-01-04 | Compumedics Limited | Sensor assembly with conductive bridge |
| EP1957653A4 (en) * | 2005-08-30 | 2009-12-30 | Phyllom Llc | TRANSGENIC PLASTIC LAYER RESISTANT TO INSECTS |
| GB0520344D0 (en) | 2005-10-06 | 2005-11-16 | Tmo Biotec Ltd | Microoganisms |
| CA2627191A1 (en) * | 2005-10-31 | 2007-05-10 | The Trustees Of Dartmouth College | Thermophilic organisms for conversion of lignocellulosic biomass to ethanol |
| TW200801513A (en) | 2006-06-29 | 2008-01-01 | Fermiscan Australia Pty Ltd | Improved process |
| DE602006017710D1 (en) | 2006-09-14 | 2010-12-02 | Tmo Renewables Ltd | Lipase |
| AU2007301758B2 (en) | 2006-09-28 | 2011-09-15 | Tmo Renewables Limited | Thermophilic microorganisms for ethanol production |
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2008
- 2008-05-09 BR BRPI0811556A patent/BRPI0811556A2/pt not_active IP Right Cessation
- 2008-05-09 KR KR1020097025740A patent/KR20100037031A/ko not_active Ceased
- 2008-05-09 CN CN200880024031A patent/CN101848924A/zh active Pending
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- 2008-05-09 RU RU2009145274/10A patent/RU2541785C2/ru not_active IP Right Cessation
- 2008-05-09 EP EP12164672A patent/EP2511286A3/en not_active Withdrawn
- 2008-05-09 CA CA002685879A patent/CA2685879A1/en not_active Abandoned
- 2008-05-09 AU AU2008251465A patent/AU2008251465B2/en not_active Ceased
- 2008-05-09 US US12/599,458 patent/US8435770B2/en not_active Expired - Fee Related
- 2008-05-09 NZ NZ581493A patent/NZ581493A/en not_active IP Right Cessation
- 2008-05-09 EP EP12164671A patent/EP2511285A3/en not_active Withdrawn
- 2008-05-09 EP EP12164673A patent/EP2511287A3/en not_active Withdrawn
- 2008-05-09 EP EP08769395A patent/EP2064225A4/en not_active Withdrawn
- 2008-05-09 WO PCT/US2008/063237 patent/WO2008141174A2/en not_active Ceased
- 2008-05-09 EP EP12164670A patent/EP2511284A3/en not_active Withdrawn
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Also Published As
| Publication number | Publication date |
|---|---|
| CN101848924A (zh) | 2010-09-29 |
| US8435770B2 (en) | 2013-05-07 |
| BRPI0811556A2 (pt) | 2017-05-02 |
| EP2511286A3 (en) | 2012-11-28 |
| CA2685879A1 (en) | 2008-11-20 |
| NZ599526A (en) | 2013-07-26 |
| US20100297721A1 (en) | 2010-11-25 |
| RU2541785C2 (ru) | 2015-02-20 |
| WO2008141174A9 (en) | 2012-07-05 |
| EP2511284A2 (en) | 2012-10-17 |
| KR20100037031A (ko) | 2010-04-08 |
| ZA200908721B (en) | 2010-08-25 |
| WO2008141174A2 (en) | 2008-11-20 |
| EP2511287A2 (en) | 2012-10-17 |
| EP2511285A3 (en) | 2012-11-28 |
| EP2511284A3 (en) | 2012-11-28 |
| AU2008251465A1 (en) | 2008-11-20 |
| US20150024450A1 (en) | 2015-01-22 |
| WO2008141174A3 (en) | 2009-03-12 |
| IL201824A0 (en) | 2010-06-16 |
| JP2010526536A (ja) | 2010-08-05 |
| EP2511286A2 (en) | 2012-10-17 |
| AU2008251465B2 (en) | 2013-01-10 |
| RU2009145274A (ru) | 2011-06-20 |
| EP2064225A4 (en) | 2010-03-03 |
| EP2064225A2 (en) | 2009-06-03 |
| EP2511285A2 (en) | 2012-10-17 |
| EP2511287A3 (en) | 2012-11-28 |
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