KR20100037031A - 유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법 - Google Patents

유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법 Download PDF

Info

Publication number
KR20100037031A
KR20100037031A KR1020097025740A KR20097025740A KR20100037031A KR 20100037031 A KR20100037031 A KR 20100037031A KR 1020097025740 A KR1020097025740 A KR 1020097025740A KR 20097025740 A KR20097025740 A KR 20097025740A KR 20100037031 A KR20100037031 A KR 20100037031A
Authority
KR
South Korea
Prior art keywords
genetically modified
microorganism
thermophilic
mesophilic
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020097025740A
Other languages
English (en)
Korean (ko)
Inventor
데이비드 에이. 호그셋
비니트 비. 라이갈하이아
Original Assignee
마스코마 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 마스코마 코포레이션 filed Critical 마스코마 코포레이션
Publication of KR20100037031A publication Critical patent/KR20100037031A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/02—Oxidoreductases acting on the CH-OH group of donors (1.1) with a cytochrome as acceptor (1.1.2)
    • C12Y101/02003—L-Lactate dehydrogenase (cytochrome) (1.1.2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008—Phosphate acetyltransferase (2.3.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01017—Xylulokinase (2.7.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02—Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02001—Acetate kinase (2.7.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020097025740A 2007-05-09 2008-05-09 유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법 Ceased KR20100037031A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91697807P 2007-05-09 2007-05-09
US60/916,978 2007-05-09

Publications (1)

Publication Number Publication Date
KR20100037031A true KR20100037031A (ko) 2010-04-08

Family

ID=40002867

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020097025740A Ceased KR20100037031A (ko) 2007-05-09 2008-05-09 유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법

Country Status (13)

Country Link
US (2) US8435770B2 (enExample)
EP (5) EP2511286A3 (enExample)
JP (1) JP2010526536A (enExample)
KR (1) KR20100037031A (enExample)
CN (1) CN101848924A (enExample)
AU (1) AU2008251465B2 (enExample)
BR (1) BRPI0811556A2 (enExample)
CA (1) CA2685879A1 (enExample)
IL (1) IL201824A0 (enExample)
NZ (2) NZ581493A (enExample)
RU (1) RU2541785C2 (enExample)
WO (1) WO2008141174A2 (enExample)
ZA (1) ZA200908721B (enExample)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9650642B2 (en) * 2008-02-08 2017-05-16 Algenol Biotech LLC Genetically modified cyanobacteria for the production of ethanol
WO2009109630A1 (en) * 2008-03-07 2009-09-11 Dsm Ip Assets B.V. A pentose sugar fermenting cell
DE102008031350B4 (de) * 2008-07-02 2011-02-10 Johann Wolfgang Goethe-Universität Frankfurt am Main Prokaryotische Xylose-Isomerase zur Konstruktion Xylose-vergärender Hefen
EP2313513A4 (en) 2008-07-28 2012-01-11 Univ Massachusetts METHOD AND COMPOSITION FOR IMPROVED MANUFACTURE OF PRODUCTS IN MICROORGANISMS
CA2743505A1 (en) * 2008-11-12 2010-05-20 Mascoma Corporation Gene knockout mesophilic and thermophilic organisms, and methods of use thereof
EP2194120A1 (en) * 2008-12-02 2010-06-09 Total S.A. Bioprocessing ligno-cellulose into ethanol with recombinant clostridium
WO2010075213A2 (en) * 2008-12-22 2010-07-01 Mascoma Corporation Production of ethanol from lignocellulosic biomass
WO2011059314A1 (en) * 2009-11-12 2011-05-19 Stichting Voor De Technische Wetenschappen Pentose transporters and uses thereof
EP2529003A2 (en) 2010-01-26 2012-12-05 Scale Biofuel APS Methods for producing and harvesting ethanol and apparatus for producing and harvesting the same
GB2478791A (en) * 2010-03-19 2011-09-21 Qteros Inc Ethanol production by genetically-modified bacteria
US8927254B2 (en) 2010-09-29 2015-01-06 University Of Georgia Research Foundation, Inc. Pyrococcus furiosus strains and methods of using same
US9546385B2 (en) 2010-12-22 2017-01-17 Enchi Corporation Genetically modified clostridium thermocellum engineered to ferment xylose
AU2012223494B2 (en) 2011-02-28 2017-04-06 Dsm Nutritional Products, Llc Polymeric acid catalysts and uses thereof
US8962333B2 (en) 2011-04-04 2015-02-24 University Of Georgia Research Foundation, Inc. Restriction/modification polypeptides, polynucleotides, and methods
AP3983A (en) 2011-05-18 2017-01-06 Scale Biofuel Aps Solar-assisted volatile fermentation products production processes
WO2013041668A1 (en) * 2011-09-22 2013-03-28 Direvo Industrial Biotechnology Gmbh Single-step bioconversion of lignocellulosic biomass to biofuels using extreme thermophilic bacteria
CA2850480A1 (en) 2011-09-30 2013-06-20 Mascoma Corporation Engineering microorganisms to increase ethanol production by metabolic redirection
CA2857498A1 (en) * 2011-11-30 2013-09-26 Mascoma Corporation Engineering an increase in ethanol production by altering cofactor specificity
US9309542B2 (en) 2012-08-17 2016-04-12 University Of Georgia Research Foundation, Inc. Recombinant Caldicellulosiruptor bescii and methods of use
US9238845B2 (en) 2012-08-24 2016-01-19 Midori Usa, Inc. Methods of producing sugars from biomass feedstocks
US9587256B2 (en) 2012-09-06 2017-03-07 University Of Georgia Research Foundation, Inc. Sequestration of carbon dioxide with hydrogen to useful products
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
MY203868A (en) 2014-06-11 2024-07-22 Univ Duke Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
CN104263680B (zh) * 2014-09-05 2017-02-01 华南理工大学 一种嗜热厌氧杆菌及利用其生产乙醇的方法
CN105154465A (zh) * 2015-07-23 2015-12-16 昆明理工大学 一种敲除乙酸激酶基因的重组质粒及其应用
CN106893745A (zh) * 2017-04-12 2017-06-27 浙江畯和生物科技有限公司 一种木质纤维素生物质的高效转化利用方法
TWI664286B (zh) * 2017-08-02 2019-07-01 國立清華大學 生產醋酸的基因轉殖藍綠菌及其應用
WO2019246488A1 (en) 2018-06-21 2019-12-26 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control
US12275962B2 (en) 2019-07-02 2025-04-15 Codexis, Inc. Engineered acetate kinase variant enzymes
CN110484466B (zh) * 2019-08-15 2023-04-21 华南理工大学 一种提高嗜热厌氧杆菌发酵性能的方法
CN111423256A (zh) * 2020-04-23 2020-07-17 上海国璨环境科技有限公司 一种有机垃圾热解方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027895A (en) 1995-09-12 2000-02-22 The Ohio State University Research Foundation Methods for cleaving DNA with nucleotide integrases
US5698421A (en) 1995-09-12 1997-12-16 The Ohio State Research Foundation Ribonucleoprotein particles for cleaving double-stranded DNA and inserting an RNA/DNA molecule into the cleavage site
DK0853127T3 (da) * 1996-09-27 2005-03-07 Dsm Ip Assets Bv Biotin-biosyntese-gener II
US5804418A (en) 1996-11-19 1998-09-08 The Ohio State University Research Foundation Methods for preparing nucleotide integrases
WO1999043854A1 (en) 1998-02-26 1999-09-02 The Ohio State Research Foundation Methods for cleaving single-stranded and double-stranded dna substrates with nucleotide integrase
GB0000185D0 (en) 2000-01-06 2000-03-01 Agrol Limited Ethanol production
GB0011186D0 (en) 2000-05-09 2000-06-28 Agrol Limited Modification of bacteria
CN1336432A (zh) * 2000-07-31 2002-02-20 武汉迪普生物技术有限公司 重组枯激酶的生产方法及其生物活性
CA2424890C (en) 2000-10-06 2014-06-03 Elsworth Biotechnology Limited Ethanol production in gram-positive bacteria with a stabilized mutation in lactate dehydrogenase
US20030165859A1 (en) 2001-10-23 2003-09-04 Invitrogen Corporation Primers and methods for the detection and discrimination of nucleic acids
US7026464B2 (en) * 2002-10-21 2006-04-11 E. I. Du Pont De Nemours And Company Natural promoters for gene expression in C1 metabolizing bacteria
RU2346996C2 (ru) 2004-06-29 2009-02-20 ЮРОПИЭН НИКЕЛЬ ПиЭлСи Усовершенствованное выщелачивание основных металлов
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
WO2006099615A2 (en) * 2005-03-16 2006-09-21 The Johns Hopkins University Adenoviral fiber exchange shuttle system
JP2008539710A (ja) 2005-05-04 2008-11-20 ティーエムオー リニューアブルズ リミティド エタノール生産用の不活性化乳酸デヒドロゲナーゼ（ｌｄｈ）遺伝子を有する好熱性微生物
GB0511602D0 (en) 2005-06-07 2005-07-13 Tmo Biotec Ltd Microorganisms
WO2007000020A1 (en) 2005-06-29 2007-01-04 Compumedics Limited Sensor assembly with conductive bridge
EP1957653A4 (en) * 2005-08-30 2009-12-30 Phyllom Llc TRANSGENIC PLASTIC LAYER RESISTANT TO INSECTS
GB0520344D0 (en) 2005-10-06 2005-11-16 Tmo Biotec Ltd Microoganisms
CA2627191A1 (en) * 2005-10-31 2007-05-10 The Trustees Of Dartmouth College Thermophilic organisms for conversion of lignocellulosic biomass to ethanol
TW200801513A (en) 2006-06-29 2008-01-01 Fermiscan Australia Pty Ltd Improved process
DE602006017710D1 (en) 2006-09-14 2010-12-02 Tmo Renewables Ltd Lipase
AU2007301758B2 (en) 2006-09-28 2011-09-15 Tmo Renewables Limited Thermophilic microorganisms for ethanol production

Also Published As

Publication number Publication date
CN101848924A (zh) 2010-09-29
US8435770B2 (en) 2013-05-07
BRPI0811556A2 (pt) 2017-05-02
EP2511286A3 (en) 2012-11-28
CA2685879A1 (en) 2008-11-20
NZ599526A (en) 2013-07-26
US20100297721A1 (en) 2010-11-25
RU2541785C2 (ru) 2015-02-20
WO2008141174A9 (en) 2012-07-05
EP2511284A2 (en) 2012-10-17
NZ581493A (en) 2012-05-25
ZA200908721B (en) 2010-08-25
WO2008141174A2 (en) 2008-11-20
EP2511287A2 (en) 2012-10-17
EP2511285A3 (en) 2012-11-28
EP2511284A3 (en) 2012-11-28
AU2008251465A1 (en) 2008-11-20
US20150024450A1 (en) 2015-01-22
WO2008141174A3 (en) 2009-03-12
IL201824A0 (en) 2010-06-16
JP2010526536A (ja) 2010-08-05
EP2511286A2 (en) 2012-10-17
AU2008251465B2 (en) 2013-01-10
RU2009145274A (ru) 2011-06-20
EP2064225A4 (en) 2010-03-03
EP2064225A2 (en) 2009-06-03
EP2511285A2 (en) 2012-10-17
EP2511287A3 (en) 2012-11-28

Similar Documents

Publication Publication Date Title
KR20100037031A (ko) 유전자 녹아웃 중온성 및 호열성 생물체, 및 이의 사용 방법
CA2834053C (en) Yeast strains engineered to produce ethanol from glycerol
CN101939434B (zh) 用于在大豆中提高种子贮藏油脂的生成和改变脂肪酸谱的来自解脂耶氏酵母的dgat基因
CN101365788B (zh) Δ-9延伸酶及其在制备多不饱和脂肪酸中的用途
AU2017220789B2 (en) Compositions and Methods for Modifying Genomes
CN108138121B (zh) 用微生物高水平生产长链二羧酸
DK3108014T3 (en) Methods and systems for rapid detection of microorganisms using recombinant bacteriophage
IL236992A (en) Genetically modified cyanobacteria that produce ethanol
DK2670846T3 (en) METHODS FOR THE DEVELOPMENT OF TERPEN SYNTHASE VARIETIES
KR20210149060A (ko) Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
KR20140113997A (ko) 부탄올 생성을 위한 유전자 스위치
KR20140092759A (ko) 숙주 세포 및 아이소부탄올의 제조 방법
DK2324120T3 (en) Manipulating SNF1 protein kinase OF REVISION OF OIL CONTENT IN OLEAGINOUS ORGANISMS
BRPI0806354A2 (pt) plantas oleaginosas transgências, sementes, óleos, produtos alimentìcios ou análogos a alimento, produtos alimentìcios medicinais ou análogos alimentìcios medicinais, produtos farmacêuticos, bebidas fórmulas para bebês, suplementos nutricionais, rações para animais domésticos, alimentos para aquacultura, rações animais, produtos de sementes inteiras, produtos de óleos misturados, produtos, subprodutos e subprodutos parcialmente processados
AU2010258955B2 (en) Expression cassettes derived from maize
KR20140099224A (ko) 케토-아이소발레레이트 데카르복실라제 효소 및 이의 이용 방법
US20040003420A1 (en) Modified recombinase
KR20130032897A (ko) 알코올 발효 시의 알코올 에스테르의 생성 및 원위치에서의 생성물 제거
CN108473968A (zh) 生产植物鞘氨醇或二氢神经鞘氨醇的方法
KR20120099509A (ko) 재조합 숙주 세포에서 육탄당 키나아제의 발현
KR20130027063A (ko) Ｆｅ-ｓ 클러스터 요구성 단백질의 활성 향상
KR20130117753A (ko) 포스포케톨라아제를 포함하는 재조합 숙주 세포
KR20220012327A (ko) 피토칸나비노이드 및 피토칸나비노이드 전구체의 생산을 위한 방법 및 세포
CN110423705A (zh) 用于通过添加交替电子受体改善微生物中的产品收率和产量的方法
KR20140146616A (ko) 부타놀로겐용 배지의 아세테이트 보충물

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20091209

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20130509

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20140730

Patent event code: PE09021S01D

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20150227

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20150507

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20150227

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I

Patent event date: 20140730

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I