NO323038B1 - Fremgangsmate for a oppna ekspresjon av et peptid av interesse i fro, fusjonspolypeptid fremstilt ifolge fremgangsmaten, kimaer DNA-konstruksjon som koder for fusjonspolypeptidet, ekspresjonskassett som omfatter den kimaere DNA-sekvensen, samt plantecelle som omfatter nevnte ekspresjonskassett. - Google Patents

Fremgangsmate for a oppna ekspresjon av et peptid av interesse i fro, fusjonspolypeptid fremstilt ifolge fremgangsmaten, kimaer DNA-konstruksjon som koder for fusjonspolypeptidet, ekspresjonskassett som omfatter den kimaere DNA-sekvensen, samt plantecelle som omfatter nevnte ekspresjonskassett. Download PDF

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Publication number
NO323038B1
NO323038B1 NO19943914A NO943914A NO323038B1 NO 323038 B1 NO323038 B1 NO 323038B1 NO 19943914 A NO19943914 A NO 19943914A NO 943914 A NO943914 A NO 943914A NO 323038 B1 NO323038 B1 NO 323038B1
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Norway
Prior art keywords
peptide
amino acid
dna sequence
oil body
gene
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NO19943914A
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English (en)
Norwegian (no)
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NO943914L (no
NO943914D0 (no
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Maurice M Moloney
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Sembiosys Genetics Inc
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Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Publication of NO943914D0 publication Critical patent/NO943914D0/no
Publication of NO943914L publication Critical patent/NO943914L/no
Publication of NO323038B1 publication Critical patent/NO323038B1/no

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/8139Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8234Seed-specific, e.g. embryo, endosperm
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6483Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23Aspartic endopeptidases (3.4.23)
    • C12Y304/23004Chymosin (3.4.23.4), i.e. rennin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Edible Oils And Fats (AREA)
  • Seasonings (AREA)
NO19943914A 1992-04-15 1994-10-14 Fremgangsmate for a oppna ekspresjon av et peptid av interesse i fro, fusjonspolypeptid fremstilt ifolge fremgangsmaten, kimaer DNA-konstruksjon som koder for fusjonspolypeptidet, ekspresjonskassett som omfatter den kimaere DNA-sekvensen, samt plantecelle som omfatter nevnte ekspresjonskassett. NO323038B1 (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CA1992/000161 WO1993021320A1 (en) 1991-02-22 1992-04-15 Oil-body proteins as carriers of high-value peptides in plants

Publications (3)

Publication Number Publication Date
NO943914D0 NO943914D0 (no) 1994-10-14
NO943914L NO943914L (no) 1994-12-09
NO323038B1 true NO323038B1 (no) 2006-12-27

Family

ID=32778493

Family Applications (1)

Application Number Title Priority Date Filing Date
NO19943914A NO323038B1 (no) 1992-04-15 1994-10-14 Fremgangsmate for a oppna ekspresjon av et peptid av interesse i fro, fusjonspolypeptid fremstilt ifolge fremgangsmaten, kimaer DNA-konstruksjon som koder for fusjonspolypeptidet, ekspresjonskassett som omfatter den kimaere DNA-sekvensen, samt plantecelle som omfatter nevnte ekspresjonskassett.

Country Status (11)

Country Link
EP (1) EP0636179B1 (ko)
JP (1) JP3639592B2 (ko)
KR (1) KR950700992A (ko)
AT (1) ATE277180T1 (ko)
CA (1) CA2118113C (ko)
DE (1) DE69233416T2 (ko)
DK (1) DK0636179T3 (ko)
ES (1) ES2227511T3 (ko)
FI (1) FI120266B (ko)
NO (1) NO323038B1 (ko)
WO (1) WO1993021320A1 (ko)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
US7091401B2 (en) 1991-02-22 2006-08-15 Sembiosys Genetics Inc. Expression of epidermal growth factor in plant seeds
US5869602A (en) 1995-03-17 1999-02-09 Novo Nordisk A/S Peptide derivatives
US5824870A (en) * 1995-11-06 1998-10-20 Baszczynski; Chris Commercial production of aprotinin in plants
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures
US5856452A (en) * 1996-12-16 1999-01-05 Sembiosys Genetics Inc. Oil bodies and associated proteins as affinity matrices
FR2757874B1 (fr) * 1996-12-17 2003-04-25 Biocem Collagenes recombinants et proteines derivees produits par les plantes, leurs procedes d'obtention et leurs utilisations
US7585645B2 (en) * 1997-05-27 2009-09-08 Sembiosys Genetics Inc. Thioredoxin and thioredoxin reductase containing oil body based products
US7008664B1 (en) 1998-06-11 2006-03-07 E. I. Du Pont De Nemours And Company Method for improving the carcass quality of an animal
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
WO2004113540A1 (en) * 2003-06-18 2004-12-29 The University Of York Expression of heterologous protein
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
WO2007045019A2 (en) * 2005-10-19 2007-04-26 Agriculture Victoria Services Pty Ltd Polyoleosins
JP2009542240A (ja) * 2006-07-11 2009-12-03 ビーエーエスエフ ソシエタス・ヨーロピア 脂質体へのタンパク質ターゲティング
CN114409729B (zh) * 2021-11-11 2023-06-20 南京财经大学 一种菜籽肽及其在制备药物纳米载体方面的应用

Also Published As

Publication number Publication date
AU1583292A (en) 1993-11-18
AU678136B2 (en) 1997-05-22
JP3639592B2 (ja) 2005-04-20
CA2118113C (en) 2002-01-08
EP0636179B1 (en) 2004-09-22
ES2227511T3 (es) 2005-04-01
DE69233416T2 (de) 2005-03-03
FI944841A (fi) 1994-12-14
FI944841A0 (fi) 1994-10-14
FI120266B (fi) 2009-08-31
DK0636179T3 (da) 2004-12-20
KR950700992A (ko) 1995-02-20
DE69233416D1 (de) 2004-10-28
NO943914L (no) 1994-12-09
JPH07507199A (ja) 1995-08-10
NO943914D0 (no) 1994-10-14
EP0636179A1 (en) 1995-02-01
WO1993021320A1 (en) 1993-10-28
CA2118113A1 (en) 1993-10-28
ATE277180T1 (de) 2004-10-15

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