NO321014B1 - Hydrolysis tank for use in a hydrolysis process for raw materials from the fish and slaughterhouse industry - Google Patents

Abstract

The invention relates to a hydrolysis tank comprising inlets for adding raw materials and outlets for products, a rotatable stirring mechanism, and a device for heat exchange, where one or more reversible screws are arranged in outlets at the bottom of the tank. Furthermore, the invention comprises a method for an enzymatic hydrolysis process of collagen and proteinaceous raw materials, wherein the raw materials are mixed by a stirring mechanism rotating, and at the same time rotating one or more reversible transport screws arranged in connection with the bottom portion of the tank inwards towards the tank. center of thought. The invention also relates to uses of said hydrolysis tank in an enzymatic hydrolysis process.

Description

The present invention relates to a method for an enzymatic hydrolysis process of collagen and proteinaceous raw materials, comprising the following steps: (1) to hydrolyze the raw materials enzymatically so that three following fractions are produced;

(a) a fatty top fraction,

(b) a middle fraction comprising water-soluble constituents, including water-soluble proteins including collagen, and (c) an insoluble bottom fraction comprising bone debris and insoluble proteins;

and

(2) to separate (a), (b) and (c).

The invention also relates to a hydrolysis tank comprising inlets for the addition of raw materials and outlets for products, a rotatable stirring mechanism, and an arrangement for heat exchange.

The invention also relates to applications of the hydrolysis tank.

Introduction

The production of foodstuffs, for example in the fishing industry and the butchering industry, creates large quantities of by-products that are very rich in valuable components, including proteins, oil and calcium. In order to safeguard these resources, several different processes have previously been developed which release the valuable components. These processes are often based on ensiling or enzymatic hydrolysis. However, this results in proteins and oil with a quality that does not meet the requirements of the food industry. They can therefore only be used for the production of animal feed.

In order to produce products that meet the quality requirements in the food industry, enzymes have previously been developed that can break down the raw material into its individual components. These enzymes enable an enzymatic hydrolysis of, for example, offal. The proteins in the raw material are then dissolved in water so that the protein, oil and bone fractions can be separated. These enzymes are commercially available.

Within the fishing industry, large quantities of by-products with a high content of valuable proteins and oils are produced. Today, there is a certain amount of activity based on ensiling to take care of this. Attempts have also been made with enzymatic hydrolysis.

The invention relates to a step in a process for operating the hydrolysis process non-continuously. Continuous hydrolysis processes already exist, but they have a number of weaknesses. Because they are continuous processes, there is a certain free flow between the thoughts/process steps. This leads to the raw material being unevenly hydrolysed. The finished hydrolyzate product contains collagen, which makes it a mixed product that has a limited area of use. Hydrolyzate produced by such processes is not suitable for human consumption.

The inventor of the present invention has developed a "baten" process to drive enzymatic hydrolysis of raw materials from the fishing industry and the butcher industry. This process produces a product that is also suitable as food for humans.

The hydrolysis process according to the present invention is carried out with

commercially available enzymes, but in a hydrolysis tank newly developed by the inventor. The hydrolysis tank has a particularly good mixing capacity, because large screws at the bottom of the tank push the contents back towards the center of the tank, so that the stirring mechanism achieves maximum efficiency. Good mixing and large heating surfaces mean that the temperature can be kept very even, so that the hydrolysis process is optimised. Since this is a closed "baten" system, i

unlike the continuous systems that are widely used today, not only the temperature can be regulated very well but also the time periods the hydrolyzate spends at different temperatures.

The hydrolysis process results in three fractions, one of which comprises water-soluble proteins, including collagen. The inventor has developed a new method to effectively separate the collagen from the remaining proteins, which is mentioned in the Norwegian patent application from which the present application is divided; 20051216. This method involves rapidly cooling down the water-soluble proteins without agitation. The collagen, which was denatured and therefore liquid at high temperatures, will then separate out at the bottom of the tank in a renatured, solid form. The remaining water-dissolved proteins can then be pumped out, and the collagen will then be heated to a denatured liquid state so that it can also be removed from the clarification tank.

In order to carry out an effective separation of collagen from the remaining water-soluble proteins in the hydrolyzate, the inventor has developed a new type of clarification tank, which is also described in Norwegian patent application no. 20051216. This tank can cool down and heat up liquid, in this case hydrolyzate, very fast and very smooth. Since the cooling must take place without agitation, stirring cannot be used to obtain an even temperature distribution. Therefore, the tank contains large cooling/heating surfaces, so that the cooling/heating surface area is very large in relation to the bag volume, and the bag cools down/heats quickly and evenly.

The method according to the invention is thus characterized by step (1) comprising mixing the raw materials during the hydrolysis by:

to rotate a stirring mechanism; and wood

to simultaneously rotate one or more reversible transport screws arranged in connection with the bottom part of the tank in the direction inwards into the tank, whereby the raw material is constantly brought into the center of the tank.

The hydrolysis tank according to the invention is characterized by the fact that one or more reversible transport screws are arranged in the outlet at the bottom of the tank.

Further embodiments of the method and the hydrolysis tank according to the invention are specified in sub-claims 2-4 and 6-7.

The applications according to the invention are characterized by using the hydrolysis tank in accordance with one or more of claims 1-4, in ensiling processes and enzymatic hydrolysis processes, and/or in a process in accordance with the method according to one or more of claims 5-7.

The invention will now be described in more detail with reference to the attached drawings, in which: Figure 1 shows a flow chart for an enzymatic hydrolysis process according to a preferred embodiment of the invention: Figure 2 shows a cross-section of a hydrolysis tank according to a preferred embodiment of the invention.

Figure 3 shows the section A-A in Figure 2 seen from the side of the hydrolysis tank.

Figure 4 shows a top section of the hydrolysis tank in Figure 2.

Figure 5 shows the hydrolysis tank in Figure 2 seen from above.

Figure 6 shows a top section of a clarification tank according to Norwegian patent application no. 20051216 Figure 7 shows the cross-section B-B of the clarification tank in Figure 6.

The hydrolysis process

The hydrolysis process can be described in more detail with reference to figure 1. The arrows in figure 1 show where raw material goes in and products come out. Raw material in the form of by-products from the fishing industry or the butchering industry is filled into a hydrolysis tank (10). Raw material from the fishing industry can be whole fish or parts of fish such as fish heads, fish bones, fish skin or fish guts. Raw materials from the fishing industry also include shellfish. Corresponding raw material from the butcher industry can also be used, including all parts of an animal, or the whole animal. The term "animal" is intended here to include birds, such as chickens. The process according to the invention can be adapted to all strut raw materials that include protein and collagen, i.e. bone, cartilage, connective tissue, and skin/skin. The raw material will typically be a mixture of different raw materials which are by-products from further processing from the industries, but it can of course consist of a single raw material.

In the hydrolysis tank(s) (10), the raw material is added to hot water. The temperature is regulated so that it is optimal for the enzymatic hydrolysis. It will therefore vary depending on which enzyme you choose to use. When the desired temperature is reached, the enzymes are added, and this starts the hydrolysis reaction. The enzymes catalyze the hydrolysis, and the result is that large parts of the proteins present in the raw material are dissolved in the water. In addition to the water-soluble proteins, oil, bones and insoluble proteins are then released.

When the raw material is sufficiently hydrolysed, the enzymes will typically be deactivated by increasing the temperature. The mixture is set aside. After a short time, clearly separated fractions then arise. At the top is an oil fraction, below this is a water-soluble fraction with the dissolved proteins, and at the bottom of the tank lies the heaviest fraction, which consists of insoluble proteins and bone remains. In the rest of the description, these fractions are referred to as the oil fraction, the hydrolyzate and the bone fraction, respectively.

In total, the hydrolysis usually takes 3-4 hours, depending on how quickly you add raw materials and remove products. The oil is first removed from the hydrolysis tank, usually by tapping, and can, for example, be separated in an oil separator (11). The hydrolyzate is then drained from the hydrolysis tank while the bone fraction remains in the hydrolysis tank. The bone fraction is finally removed from the bottom of the hydrolysis tank.

After removal from the hydrolysis tank, the hydrolyzate is filtered through a conventional filter (12). The last remains of oil and bones are then removed from the hydrolyzate in a cascade tank (13), followed by a separator (14).

From the separator, the hydrolyzate flows on to a clarification tank (15). In this tank, the temperature is reduced without agitation so that the collagen is precipitated as gelatin, i.e. in a coagulated form. Which temperature is sufficiently low enough for the collagen to harden will vary with different raw materials, and often reflects the body temperature of the fish/animal/bird it originates from. Collagen from fish therefore hardens at lower temperatures than collagen from animals. Usually the hydrolyzate must be cooled to 10-25°C for fish, preferably 20-22°C, 25-40°C for animals, preferably 32-35<*>C, and 30-45°C for birds, preferably 33-40° C, for the collagen to harden. The collagen has a higher density than the rest of the hydrolyzate and will therefore sink. Two clearly separated phases then occur in the tank. On the bottom is the collagen in solid form, above this is the rest of the hydrolyzate in a liquid phase. The collagen hardens or partially hardens because the temperature is reduced.

After decanting the liquid phase into a buffer tank (16), the temperature in the tank is increased so that the collagen also liquefies and can be removed from the tank, usually by pumping and draining it. How much the temperature is increased depends on what the collagen is to be used for. If it is to be further preserved, the collagen is drained as soon as it becomes liquid. The temperature at which this happens obviously varies according to the raw materials used to produce the collagen, usually this temperature is 10-25°C more than the temperature to which the hydrolyzate was cooled to separate the collagen. If the collagen is not to be preserved, but used for other purposes, it must be heated to at least 65°C before draining from the clarification tank to avoid microbial bloom.

The hydrolyzate with collagen removed can now be further processed with methods known from other areas of application. Preferably, the hydrolyzate is processed in an evaporator (17), so that the dry matter content is increased to the desired level. When the collagen has been removed, the dry matter level is usually 3-15%, preferably around 7%. How much you choose to evaporate depends on what the hydrolyzate is to be used for. If, for example, a hydrolyzate from fish is to be returned to the fish by injecting it into the fish meat, it must have exactly the same dry matter content as the meat it is to be injected into, which is normally around 15%. If you evaporate the hydrolyzate to around 60%, it becomes self-preserving, which is of course an advantage for long-term storage. But the more you want to evaporate the hydrolyzate, the more expensive it is, and this usually sets the limit for how much the hydrolyzate is evaporated. It is crucial for the evaporation process that the collagen is separated from the hydrolyzate. Hydrolyzate without collagen removed has a viscosity that is too high, so the evaporator cannot work. By removing the collagen in the process according to the invention, the protein-containing hydrolyzate can be further processed into a dry substance with better storability which is very applicable in many areas, including food for humans and animals.

The hydrolyzate can of course be used immediately after evaporation, and in that case will be transferred to a product mixer (18), where it is mixed with other ingredients. The collagen can now be returned to the hydrolyzate if this is desired. You then do not obtain the same product as if the collagen had not been removed from the rest of the hydrolyzate, because a large proportion of the water was removed in the evaporator. The product is therefore much more concentrated and easier to store and transport. The collagen does not of course need to be returned to the hydrolyzate, since after it has been removed from the tank it can be kept separately and used for other purposes.

Figure 1. shows a process comprising two hydrolysis tanks, two clarification tanks, and two buffer tanks, but there is nothing to prevent using only one or more of these tanks. The tanks represent areas where the process bag will stay for a time, so it may be beneficial, but not necessary, to have several tanks of the various types to maximize the capacity of the hydrolysis plant.

The hydrolysis tank

In order for the hydrolysis to be as complete as possible, in the shortest possible time, the design of the hydrolysis tank is important. The contents of the tank must be able to be watered up evenly and effectively, which is why agitation is important. This is achieved by stirring, so that the temperature is uniform throughout the tank. Agitation is made more efficient by a screw at the bottom of the tank pushing larger bones, etc., which tend to settle at the bottom into the center of the tank, where they are brought into contact with the stirring mechanism. In addition to creating an even temperature distribution, the stirring also helps to give the enzymes physical access to all the raw material. To achieve this, the inventor has constructed a new type of hydrolysis tank, described in more detail in figures 2-6.

The hydrolysis tank (10) can of course vary in size according to need. In the embodiment of the invention described here, the tank has a capacity of 25,000 litres. This is a tank of a size that can be constructed centrally and then transported out on narrow roads. But there is nothing in the way of making smaller tanks for small fish farms, or larger tanks for other types of shipping, or very large tanks for use where they are constructed, and the dimensions can of course be changed.

At the bottom of the hydrolysis tank (10) there are one or more outlets (20) for removing the bone fraction. The outlets (20) are preferably located in depressions in the tank bottom, where they are inclined in relation to the horizontal plane. The angle from the outlets to the horizontal plane can be 5-45°, preferably 15-30°, preferably 20°. In this way, gravity can help when bone remains are to be carried out through

the outlets, but the angle is also small enough that the screws (21) in the outlets can help push the raw materials towards the center of the tank during the heating and mixing of raw materials, enzymes and water. The outlets contain screws (21) that can be driven in both directions. At the end of the outlets there are outlet valves (22). The outlets are enclosed by heating jackets (23). The size of the outlets can of course vary. They must be large enough to transport out the bone remains, which can vary in size depending on the raw materials used. In the preferred embodiment shown in Figures 2-5, an outlet of 200mm diameter is used.

The hydrolysis anchor has one or more inlets for steam (24), and one or more inlets

outlet for steam and condensation (25). The side walls (26), the bottom (27) and the inner cylinder (28) are equipped with heating jackets (29). It is therefore great

contact surface between process steam and the contents of the tank, which is defined by the bottom (27), the side walls (26), the inner cylinder (28) and the roof (30) of the tank.

The hydrolysis anchor can be mounted on different undercarriages, but a preferred design is 6 legs (31) with adjustable height.

Temperature and level transmitter (32) is installed so that you can monitor the temperature in the hydrolyzate itself. A stirrup (33) is fitted to facilitate access to the roof of the hydrolysis tank.

An inlet cover (34) is mounted on the roof (30) of the tank for access to the inside of the tank. There is also an inlet (35) where raw material, water and enzymes are added. A valve with a hood (36) is also mounted on the roof for venting the tank. A bridge (37) is arranged above the tank, on which a motor (38) is mounted which drives the stirring mechanism. The bridge (37) is both for structural support and for fitting pipework.

The stirring mechanism comprises the motor (38) which drives a rotary shaft (39) connected to rotary shafts (40), and is further connected to the stirring rod (41) which is connected to the stirring blades (42) themselves. The stirring blades (42) are mounted in the bottom of the tank, so that they stroke directly over the bottom when in use. As can be seen from figure 5, this preferred embodiment of the invention has three stirring blades, but one can of course use more. A support shaft (43) can also be fitted from the stirring rod (41) to the inner cylinder (28) to provide extra support for the stirring mechanism.

During a normal work cycle, the tank is first filled with hot water and raw material via the inlet (35). This is mixed by the stirring mechanism rotating, and at the same time the screws (21) at the bottom of the tank rotate in the direction into the tank (that is, in the opposite direction to the direction used to empty the tank) so that the raw material is constantly brought into the center of the tank. The screws are new, and have not previously been used in hydrolysis tanks. This provides a very efficient mixture of water and raw material and a uniform temperature throughout the volume.

While the mixing is in progress, the temperature is regulated by letting steam into the heating jackets. When water and raw material are mixed, enzymes are added, still stirring. The reaction then starts immediately. When the reaction is complete, the enzymes are inactivated by letting more steam into the heating mantles and the mixture reaches the enzyme's inactivation temperature. In the tank there is very good temperature control. This is achieved by a combination of large heating surfaces and efficient stirring.

After the inactivation temperature for the enzymes used has been reached, the stirring is stopped and the fractions are separated, based on specific gravity. Outlets for oil draining (44) are mounted at different heights, so that you can drain from the outlets that are most appropriate. Often, one will first use an outlet a little above the transition between the oil fraction and the hydrolyzate, to prevent the hydrolyzate from swirling up, and then towards the end of bottling use the outlet that is at the bottom of the oil fraction. Depending on the type of raw material used, the amount of oil will vary, as it is desirable to have outlets at different heights, so that as much oil as possible can be drained off without draining the hydrolysis fraction. Figure 3 shows these outlets seen directly towards the tank, from section A-A in Figure 2. Figure 3 also shows windows (45) through which one can see into the tank. Thereby, one can see how high the oil level is, and from this decide which of the outlets (44) it is most appropriate to use. A window with a light source (46) which is mounted on the lid of the tank can also be used to see the inside of the tank.

After the oil extraction, the hydrolyzate is drained off over the bone fraction. If the level in the tank is very high, this can happen first through the lower outlets for oil (44). Outlet for the hydrolyzate (47) is then used. It is desirable to use outlets as high as possible on the tank, and not start with the outlets near the bottom, because this can disrupt the separation of the layers so that bone remains from the bottom fraction are swirled up in the hydrolyzate. The same applies to the oil drain. If there is little bone residue, so that the hydrolyzate comes below the lowest of the outlets (47), this can possibly be pumped through the screws (21). The larger bone remains can then act as a sieve and prevent small bone particles from accompanying the hydrolyzate. Finally, the bone fraction itself is removed via the screws (21). The direction the shed (21) rotates is then the opposite of the direction during the stirring, they now push the bone remains out of the tank. When this fraction is removed, the stirrer is also running so that the mass falls into the openings where the screws are placed. This is very rational since the fraction is very demanding to pump.

Conventional hydrolysis systems also use screws, but for a completely different purpose. The screws are continuously driven in one direction to constantly advance the hydrolyzate. The hydrolyzate thus passes through different temperature zones, and the idea is that it should pass evenly through the system and spend the desired time in the different temperature zones. In practice, however, it does not quite work that way, there is a lot of free flow inside the screws, so that the hydrolyzate does not pass evenly through the system, and does not spend optimal time in the different temperature zones.

Although this tank was constructed as part of an enzymatic hydrolysis process, its application is not limited to enzymatic hydrolysis. The tank is also very suitable for hydrolysis during ensiling. The same raw materials are then used as described for enzymatic hydrolysis, but instead of adding water and enzymes, water and acid are added, possibly with additional chemicals. The hydrolysis process then takes place under high temperatures, and like enzymatic hydrolysis, it is important to have a uniform temperature and good mixing. By using this tank, you get a high temperature that can be kept constant throughout the separation phase, in contrast to the systems used today, where you get big temperature fluctuations because you use a pre-heater to heat up the raw materials before they are ensiled.

The clearance tank

The collagen in the hydrolysis fraction is separated from the remaining water-soluble proteins in the clarification tank by precipitation. This process is new, and the clarification tank has been newly developed to carry out the process. The clarification tank and the process are not part of the present invention, but belong to Norwegian patent application 20051216, from which the present invention is separated. The description thereof is nevertheless retained here, as it is most optimal to use both the hydrolysis tank and the clarification tank in the same system. The concept is simple: if the hydrolyzate is allowed to stand still at a temperature low enough for the collagen to separate and solidify in a bottom layer, the rest of the hydrolyzate, which will remain liquid after the collagen has solidified, can be drained off and thereby separated from the collagen. The collagen layer can then be heated so that it becomes liquid again, and then drained.

Two factors are particularly important for the separation of the collagen to be successful, and the clarification tank is designed with this in mind. Firstly, it is important that the hydrolyzate is allowed to stand completely still for the collagen to solidify. One cannot therefore use stirring or any other form of agitation to achieve the necessary temperature distribution in the hydrolyzate. It is important that the temperature is the same throughout the tank. If the temperature distribution is poor, collagen in different areas of the tank will solidify at different times. Some of the remaining hydrolyzate may even freeze before all the collagen has solidified, so it cannot be drained. Secondly, the time perspective is important, so that the process does not stop at this stage, but most of all because microorganisms can easily flourish and destroy the product. In order for the process to be approved for the preparation of food for humans, it is therefore very important that the cooling and heating can take place quickly.

The clarification tank is therefore constructed with a large number of hot/cooling surfaces so that the contents of the tank can be cooled down quickly without agitation. By surfaces is meant the surfaces that are in contact with the hydrolyzate. Figure 6 shows a section of the clarification tank seen from above. Water or steam at different temperatures is used to cool or heat the contents of the tank. The water flows into the inlet (60) and out again into the outlet (61). The water cools/heats both a jacket on the outside of the clarification tank (62), and water-filled cooling/heating surfaces inside the tank (63). It is these cooling/heating surfaces inside the tank that make it possible to achieve rapid cooling and heating of the hydrolyzate and collagen. Figure 7 shows a section of the clarification tank seen from the side, where you can see that the cooling/heating surfaces (63) are large surfaces that extend over a large part of the height of the tank.

The cooling/heating surfaces (63) are hollow plates so that the water can flow through them, preferably with grooves or ribs to further increase the surface area. This results in a large surface for heat exchange between the water flowing through the heating surfaces and the hydrolyzate inside the tank. The number of cooling/heating surfaces can of course vary, and so can the dimensions of these and the clarification tank itself. What is important is that the surface of the cooling/heating surfaces is large in relation to the volume of the hydrolyzate. The cooling/heating surfaces can be wavy to increase the surface area. The cooling/heating surfaces must be vertical, if they are horizontal the collagen can lie on top of them, instead of falling to the bottom of the tank. If only heating jackets are used on the sides and bottom of the tank, and no cooling/heating surfaces inside the tank, the cooling takes place too late. You can't just use colder water to make the cooling go faster, because water will of course freeze, but even if you use another colder bag or gas, or run the water faster through the jackets, this will then lead to an uneven temperature distribution, so that the tank contents near the walls become too cold and freeze before they solidify in the middle of the tank. The problem with allowing the cooling to take longer is that you very often have problems with microbial contamination, because the contents of the tank then spend too long at a temperature that is favorable for such microbial growth. When using the clarification tank i, the clarification process will usually take less than two hours, often around 1 Vfc hour.

The size of the clarification tank itself can of course vary in the same way as the size of the hydrolysis tank can vary.

When the clarification tank is first filled with the hydrolyzate, it can maintain a high temperature. The temperature will vary, depending on which enzyme deactivation temperature was used during the hydrolysis, how long it took from the hydrolyzate being removed from the hydrolysis tank to the addition of the clarification tank, etc. But usually it will be high, preferably 80-100'C. To cool the hydrolyzate down quickly, cold water is circulated through the cooling jacket and in the cooling plates, and when the temperature is sufficiently low, the collagen changes consistency from liquid to gel-like and sinks to the bottom of the tank. When a clear boundary has been established between the collagen and the liquid comprising the remaining water-soluble proteins, the liquid is drained off.

Like the hydrolysis tank, the clarification tank has an inlet but several outlets for liquids. The outlets, like the outlets for oil and hydrolyzate from the hydrolysis tank, are located at different heights on the tank. When the liquid comprising the remaining water-soluble proteins is to be drained, an outlet is used which is located above the collagen. When the collagen is drained, an outlet is used near or at the bottom of the tank, so that it can be emptied completely.

When the liquid comprising the remaining water-soluble proteins has been removed, the collagen can be heated again by circulating hot water in the cooling jacket and the cooling/heating surfaces. The collagen then returns to liquid form and can be pumped out of the tank for further processing.

Claims (9)

1. A hydrolysis tank comprising an inlet for the addition of raw materials and an outlet for products, a rotatable stirring mechanism, and an arrangement for heat exchange, characterized in that one or more reversible transport screws are arranged in the outlet at the bottom of the tank. 2. Hydrolysis tank in accordance with claim 1, characterized in that the transport screw(s) is arranged in a recess(es) in the bottom part of the tank and connected to the tank bottom via a slot(s). 3. Hydrolysis tank in accordance with one or more of the preceding requirements, characterized in that the direction of rotation of the reversible screw(s) is designed to lead insoluble components from the precipitation in the screw(s)' operating area(s) out of the hydrolysis tank . 4. Hydrolysis tank in accordance with one or more of the preceding requirements, characterized in that the direction of rotation of the reversible screw(s) is designed to be reversed for the return of components from the deposition(s) in the screw(s)' area of operation ( r) back to the tank volume. 5. Method for an enzymatic hydrolysis process of collagen and proteinaceous raw materials, comprising the following steps: (1) hydrolyzing the raw materials enzymatically so that three following fractions are produced; (a) a fat-containing top fraction, (b) a middle fraction comprising water-soluble constituents, including water-soluble proteins including collagen, and (c) an insoluble bottom fraction comprising bone debris and insoluble proteins; and (2) to separate (a), (b) and (c); characterized in that step (1) comprises mixing the raw materials during the hydrolysis by: rotating a stirring mechanism; and by simultaneously rotating one or more reversible transport screws arranged in connection with the bottom part of the tank in the direction inwards into the tank, whereby the raw material is constantly brought into the center of the tank. 6. Method in accordance with claim 5, characterized in that step (2) comprises removing the insoluble fraction (c) by reversing the direction of rotation of the transport screw(s) in the direction out of the tank. 7. Method in accordance with claim 5, characterized in that the raw materials are by-products from the fishing industry and/or the butchering industry. 8. Application of the hydrolysis tank in accordance with one or more of claims 1 -4, in ensiling processes and the enzymatic hydrolysis process 9. Use of the hydrolysis tank in accordance with one or more of claims 1-4, in a process in accordance with the method according to one or more of claims 5-7.