NO20091875L - Genetic ablation of PRP gene cells using a smelly serum production strategy - Google Patents

Genetic ablation of PRP gene cells using a smelly serum production strategy

Info

Publication number
NO20091875L
NO20091875L NO20091875A NO20091875A NO20091875L NO 20091875 L NO20091875 L NO 20091875L NO 20091875 A NO20091875 A NO 20091875A NO 20091875 A NO20091875 A NO 20091875A NO 20091875 L NO20091875 L NO 20091875L
Authority
NO
Norway
Prior art keywords
smelly
prp gene
genetic ablation
production strategy
gene cells
Prior art date
Application number
NO20091875A
Other languages
Norwegian (no)
Inventor
Carola Schroder
Elisabeth Casademunt
Kim Bjornstrup
Original Assignee
Octagene Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octagene Gmbh filed Critical Octagene Gmbh
Publication of NO20091875L publication Critical patent/NO20091875L/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/92—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Foreliggende oppfinnelse tilveiebringer en prionprotein (PrP)-fri immortalisert somatisk human cellelinje hvor begge allelene av PrP-genet har blitt fullstendig deletert ved homolog rekombinasjon. Oppfinnelsen tilveiebringer videre en fremgangsmåte for fremstilling av nevnte cellelinje og anvendelse derav for fremstilling av humane rekombinante proteiner som er egnet som biofarmasøytiske midler.The present invention provides a prion protein (PrP) -free immortalized somatic human cell line in which both alleles of the PrP gene have been completely deleted by homologous recombination. The invention further provides a process for the preparation of said cell line and its use for the production of human recombinant proteins suitable as biopharmaceuticals.

NO20091875A 2006-11-20 2009-05-13 Genetic ablation of PRP gene cells using a smelly serum production strategy NO20091875L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06124427 2006-11-20
PCT/EP2007/062597 WO2008061995A2 (en) 2006-11-20 2007-11-20 Genetic ablation of the prp gene cells using a targeted promoter trap strategy for production of serum-free recombinant proteins as therapeuticals

Publications (1)

Publication Number Publication Date
NO20091875L true NO20091875L (en) 2009-07-28

Family

ID=37685785

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20091875A NO20091875L (en) 2006-11-20 2009-05-13 Genetic ablation of PRP gene cells using a smelly serum production strategy

Country Status (12)

Country Link
EP (1) EP2084269A2 (en)
KR (1) KR20090106474A (en)
CN (1) CN101605891A (en)
AU (1) AU2007324530A1 (en)
BR (1) BRPI0718989A2 (en)
CA (1) CA2670003A1 (en)
IL (1) IL198770A0 (en)
MX (1) MX2009005277A (en)
NO (1) NO20091875L (en)
RU (1) RU2009123458A (en)
WO (1) WO2008061995A2 (en)
ZA (1) ZA200903429B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2728900T3 (en) * 2010-05-12 2019-10-29 Janssen Biotech Inc Differentiation of human embryonic stem cells
CN102277379B (en) * 2011-08-18 2013-07-24 中国科学院遗传与发育生物学研究所 Expression vector for expressing coagulation factor VIII and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
ATE288484T1 (en) * 1991-11-14 2005-02-15 Charles Weissmann TRANSGENIC NON-HUMAN ANIMALS WITHOUT PRION PROTEIN
ATE309328T1 (en) * 1998-12-22 2005-11-15 Univ California SOMATIC CELLS WITH DEFUNCTIONAL PRP GENE AND METHOD OF USE
BR0109521A (en) * 2000-03-24 2003-06-10 Univ Massachusetts Public Inst Transgenic ungulates without prions
AU2002242854A1 (en) * 2001-03-21 2002-10-03 Geron Corporation Use of telomerase reverse transcriptase to create homozygous knockout animals

Also Published As

Publication number Publication date
ZA200903429B (en) 2010-04-28
IL198770A0 (en) 2011-08-01
AU2007324530A1 (en) 2008-05-29
WO2008061995A3 (en) 2008-07-10
MX2009005277A (en) 2009-05-28
BRPI0718989A2 (en) 2014-02-11
CA2670003A1 (en) 2008-05-29
RU2009123458A (en) 2010-12-27
EP2084269A2 (en) 2009-08-05
CN101605891A (en) 2009-12-16
KR20090106474A (en) 2009-10-09
WO2008061995A2 (en) 2008-05-29

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