NO20054554L - Dyremodell for rask identifikasjon av farmasoytiske aktive forbindelser in vivo - Google Patents

Dyremodell for rask identifikasjon av farmasoytiske aktive forbindelser in vivo

Info

Publication number
NO20054554L
NO20054554L NO20054554A NO20054554A NO20054554L NO 20054554 L NO20054554 L NO 20054554L NO 20054554 A NO20054554 A NO 20054554A NO 20054554 A NO20054554 A NO 20054554A NO 20054554 L NO20054554 L NO 20054554L
Authority
NO
Norway
Prior art keywords
tumor
protein
expression
reporter gene
growth
Prior art date
Application number
NO20054554A
Other languages
English (en)
Other versions
NO334878B1 (no
NO20054554D0 (no
Inventor
Janine Arts
Ann Trudo Jose Belien
Ann Odette Adolf Marien
Annemie Francine Valckx
Original Assignee
Janssen Pharmaceutica Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Pharmaceutica Nv filed Critical Janssen Pharmaceutica Nv
Publication of NO20054554D0 publication Critical patent/NO20054554D0/no
Publication of NO20054554L publication Critical patent/NO20054554L/no
Publication of NO334878B1 publication Critical patent/NO334878B1/no

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/0004—Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for diabetes, allergy, rheuma, pancreas functions
    • A61K49/0008—Screening agents using (non-human) animal models or transgenic animal models or chimeric hosts, e.g. Alzheimer disease animal model, transgenic model for heart failure
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4738—Cell cycle regulated proteins, e.g. cyclin, CDC, INK-CCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests

Abstract

I et første aspekt fremskaffer foreliggende oppfinnelse en tumorcellelinje som er stabilt transfektert med en ekspresjonsvektor inneholdende et reportergen, fortrinnsvis et fluorescent protein, operativt knyttet til en promoter som også kontrollerer ekspresjon av et protein som er assosiert med tumorregresjon, stabilisering av tumorvekst eller inhibering av metastatisk vekst, og som er særpreget ved at nevnte cellelinje er i stand til å danne en tumor når implantert eller injisert i det ikke-humane dyr. Sammenlignet med tradisjonelle in vivo-modeller er foreliggende oppfinnelse forskjellig ved at reportergenet ikke blir konstituitivt uttrykt, men kun etter eksponering til en forsøksforbindelse som resulterer i ekspresjonen av et protein eller enzym som er assosiert med tumorregresjon, stabilisering av tumorvekst eller inhibering av metastatisk vekst. Kun når en forbindelse som skal undersøkes, kommer inn i sirkulasjonen og infiltrerer tumoren, kan den generere reportersignalet, forutsatt at den fremmer ekspres3onen av et protein assosiert med tumorregresjon, og promoteren av nevnte protein er operativt knyttet til reportergenet.
NO20054554A 2003-03-05 2005-10-04 Dyremodell for rask identifikasjon av farmasøytiske aktive forbindelser in vivo NO334878B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP2003/002264 WO2004078984A1 (en) 2003-03-05 2003-03-05 Animal model for the fast identification of pharmaceutical active compounds in vivo
PCT/EP2004/002195 WO2004078985A1 (en) 2003-03-05 2004-03-03 Animal model for the fast identification of pharmaceutical active compounds in vivo

Publications (3)

Publication Number Publication Date
NO20054554D0 NO20054554D0 (no) 2005-10-04
NO20054554L true NO20054554L (no) 2005-11-17
NO334878B1 NO334878B1 (no) 2014-06-30

Family

ID=32946821

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20054554A NO334878B1 (no) 2003-03-05 2005-10-04 Dyremodell for rask identifikasjon av farmasøytiske aktive forbindelser in vivo

Country Status (13)

Country Link
US (1) US7666670B2 (no)
EP (1) EP1601775B1 (no)
JP (1) JP4545142B2 (no)
CN (2) CN102766602A (no)
AT (1) ATE516359T1 (no)
AU (1) AU2004217817B2 (no)
CA (1) CA2516519C (no)
DK (1) DK1601775T3 (no)
ES (1) ES2368328T3 (no)
IL (1) IL170611A (no)
NO (1) NO334878B1 (no)
NZ (1) NZ542577A (no)
WO (2) WO2004078984A1 (no)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2594814A1 (en) 2005-01-13 2006-07-20 Senex Biotechnology, Inc. High-content screening for drugs against cancer and age-related diseases
FR2893525B1 (fr) * 2005-11-23 2008-01-18 Cogema Couteau a lame retractile, utilisable notamment en milieu hostile
US20100197767A1 (en) * 2007-07-11 2010-08-05 Lior Nissim Nucleic acid construct systems capable of daignosing or treating a cell state
SI23294A (sl) * 2010-02-25 2011-08-31 Nacionalni inštitut za biologijo Celiäśni sistem za hitro zaznavanje in doloäśanje genotoksiäśnosti
CN108744262A (zh) * 2010-11-23 2018-11-06 普莱萨格生命科学公司 用于实体递送的治疗方法和组合物
CN114350604A (zh) * 2021-12-30 2022-04-15 华南理工大学 Mc38-n4/ot-i共培养系统筛选方法及其筛选的多糖组方与多糖组合物的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994016080A1 (en) * 1993-01-08 1994-07-21 Exemplar Corporation An in vitro/in vivo method for identifying anti-neoplastic drugs
US5650135A (en) * 1994-07-01 1997-07-22 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
JP2003527833A (ja) * 1999-10-14 2003-09-24 クロンテック・ラボラトリーズ・インコーポレーテッド 花虫類に由来する発色団／蛍光体、およびそれらの使用法
AU2002237261A1 (en) * 2001-01-09 2002-07-24 Novartis Pharma Gmbh Rapid method for screening compounds for in vivo activity
US20030228627A1 (en) * 2002-03-22 2003-12-11 Emerson Beverly M. Assay for p53 function in cells

Also Published As

Publication number Publication date
JP2007523606A (ja) 2007-08-23
NO334878B1 (no) 2014-06-30
CN102766602A (zh) 2012-11-07
WO2004078984A1 (en) 2004-09-16
US20060147377A1 (en) 2006-07-06
CN1756846A (zh) 2006-04-05
ATE516359T1 (de) 2011-07-15
ES2368328T3 (es) 2011-11-16
EP1601775B1 (en) 2011-07-13
NZ542577A (en) 2007-12-21
AU2004217817B2 (en) 2009-12-10
WO2004078985A1 (en) 2004-09-16
US7666670B2 (en) 2010-02-23
AU2004217817A1 (en) 2004-09-16
CA2516519A1 (en) 2004-09-16
IL170611A (en) 2013-10-31
DK1601775T3 (da) 2011-10-31
CA2516519C (en) 2013-09-17
NO20054554D0 (no) 2005-10-04
JP4545142B2 (ja) 2010-09-15
EP1601775A1 (en) 2005-12-07

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