NO173145B - PROCEDURE FOR TREATMENT OF FEEDS - Google Patents
PROCEDURE FOR TREATMENT OF FEEDS Download PDFInfo
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- NO173145B NO173145B NO884635A NO884635A NO173145B NO 173145 B NO173145 B NO 173145B NO 884635 A NO884635 A NO 884635A NO 884635 A NO884635 A NO 884635A NO 173145 B NO173145 B NO 173145B
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- wort
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- 238000000034 method Methods 0.000 title claims description 18
- 238000011282 treatment Methods 0.000 title description 9
- 238000000108 ultra-filtration Methods 0.000 claims description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 12
- 150000004676 glycans Chemical class 0.000 claims description 11
- 229920001282 polysaccharide Polymers 0.000 claims description 11
- 239000005017 polysaccharide Substances 0.000 claims description 11
- 239000011780 sodium chloride Substances 0.000 claims description 11
- GJCOSYZMQJWQCA-UHFFFAOYSA-N 9H-xanthene Chemical compound C1=CC=C2CC3=CC=CC=C3OC2=C1 GJCOSYZMQJWQCA-UHFFFAOYSA-N 0.000 claims description 8
- 229920002305 Schizophyllan Polymers 0.000 claims description 8
- 238000000855 fermentation Methods 0.000 claims description 8
- 230000004151 fermentation Effects 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 229920001285 xanthan gum Polymers 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- FEBUJFMRSBAMES-UHFFFAOYSA-N 2-[(2-{[3,5-dihydroxy-2-(hydroxymethyl)-6-phosphanyloxan-4-yl]oxy}-3,5-dihydroxy-6-({[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}methyl)oxan-4-yl)oxy]-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl phosphinite Chemical compound OC1C(O)C(O)C(CO)OC1OCC1C(O)C(OC2C(C(OP)C(O)C(CO)O2)O)C(O)C(OC2C(C(CO)OC(P)C2O)O)O1 FEBUJFMRSBAMES-UHFFFAOYSA-N 0.000 claims description 5
- 229910052783 alkali metal Inorganic materials 0.000 claims description 4
- 229920002301 cellulose acetate Polymers 0.000 claims description 4
- WDQLRUYAYXDIFW-RWKIJVEZSA-N (2r,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-4-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-6-[[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxy-6-(hydroxymethyl)oxane-2,3,5-triol Chemical compound O[C@@H]1[C@@H](CO)O[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1 WDQLRUYAYXDIFW-RWKIJVEZSA-N 0.000 claims description 3
- -1 alkali metal salt Chemical class 0.000 claims 1
- 239000000243 solution Substances 0.000 description 26
- 229920000642 polymer Polymers 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 4
- 150000001340 alkali metals Chemical class 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000011026 diafiltration Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 241001530056 Athelia rolfsii Species 0.000 description 2
- 241000589634 Xanthomonas Species 0.000 description 2
- 241000589636 Xanthomonas campestris Species 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000222481 Schizophyllum commune Species 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229920003086 cellulose ether Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/06—Xanthan, i.e. Xanthomonas-type heteropolysaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Game Rules And Presentations Of Slot Machines (AREA)
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- Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)
Description
Foreliggende oppfinnelse gjelder en fremgangsmåte for behandling av en fermenteringsvørter som inneholder polysakkarid for å øke dens filtrerbarhet. Den resulterende, forbedrede vørter kan brukes som fortykningsmiddel ved assistert oljeutvinning. The present invention relates to a method for treating a fermentation wort containing polysaccharide to increase its filterability. The resulting improved wort can be used as a thickener in assisted oil extraction.
Polysakkaridvørteren oppnås ved hjelp av en velkjent fermenteringsprosess: en mikroorganisme som produserer polysakkarid, som f.eks. tilhører Xanthomonas-arten for fremstilling av xantan, dyrkes i et vandig næringsmedium inneholdende et karbohydrat og mer spesielt glukose. The polysaccharide wort is obtained by means of a well-known fermentation process: a microorganism that produces polysaccharide, such as belonging to the Xanthomonas species for the production of xanthan, is grown in an aqueous nutrient medium containing a carbohydrate and more specifically glucose.
Xanthomonas Campestris kan f.eks. anvendes for fremstilling av xantan. For å fremstille skleroglukan kan Sclerotium Rolfsii anvendes, og for skizofyllan vil Schizophyllum Commune-soppen bli anvendt. Xanthomonas Campestris can e.g. used for the production of xanthan. To produce scleroglucan, Sclerotium Rolfsii can be used, and for schizophyllan, the Schizophyllum Commune fungus will be used.
Ved slutten av fermenteringsprosessen oppnås en fermen-teringsvørter omfattende ca. 0,5-4% polysakkarid, såvel som salter, gjenværende naeringselementer, soppceller eller -rester og andre uløselige forbindelser. At the end of the fermentation process, a fermentation wort comprising approx. 0.5-4% polysaccharide, as well as salts, remaining nutrients, fungal cells or residues and other insoluble compounds.
De uløselige partiklene i den rå vørteren kan fjernes ved filtrering. Det er fortsatt noen makromolekylaggregater med en pseudoplastisk oppførsel som er vanskelige å filtrere og har en tendens til å tette igjen porene i underjordiske avsetninger under de assisterte utvinningsoperasjonene. The insoluble particles in the raw wort can be removed by filtration. There are still some macromolecular aggregates with a pseudoplastic behavior that are difficult to filter and tend to clog the pores of underground deposits during the assisted recovery operations.
Mange løsninger er foreslått når det gjelder dette punktet, slik det fremgår f.eks. av den betydelige patent-litteraturen. Many solutions have been proposed when it comes to this point, as can be seen e.g. of the considerable patent literature.
US-patent 3.355.447 foreslår f.eks. å oppvarme en xantan-vørter og filtrere den. US-patent 4.299.825 foreslår oppvarming og så ultrafiltrering av vørteren for å oppnå en klarnet og konsentrert vørter. EP-patent 0049012 foreslår å utføre en ultrafiltrering, med eller uten en enzymatisk behandling, for å oppnå en konsentrert xantan-løsning. US patent 3,355,447 suggests e.g. to heat a xanthan wort and filter it. US patent 4,299,825 suggests heating and then ultrafiltration of the wort to obtain a clarified and concentrated wort. EP patent 0049012 suggests performing an ultrafiltration, with or without an enzymatic treatment, to obtain a concentrated xanthan solution.
EP-patenter 0140724 og 0140725 foreslår en oppvarming, fulgt av en konsentrering ved ultrafiltrering og eventuelt diafiltrering. Det angis at ultrafiltreringsprosessen ikke har noen innvirkning på xantanløsningenes filtrerbarhet eller inj iserbarhet. EP patents 0140724 and 0140725 suggest a heating, followed by a concentration by ultrafiltration and possibly diafiltration. It is stated that the ultrafiltration process has no effect on the filterability or injectability of the xanthan solutions.
Produktene fra de nevnte prosessene virker ikke helt godt. En fremgangsmåte som gjør det mulig å oppnå en vørter med god filtrerbarhet og fremfor alt en filtrerbarhet som er holdbar, er derfor nødvendig. The products from the aforementioned processes do not work very well. A method which makes it possible to obtain a wort with good filterability and, above all, a filterability which is durable, is therefore necessary.
Fremgangsmåten ifølge oppfinnelsen for behandling av poly-sakkaridvørtere omfatter: a) vørteren underkastes først en ultrafiltrering gjennom et ultrafiltreringsdiafragma, hvis avskjæringssone er mellom The method according to the invention for treating polysaccharide worts comprises: a) the wort is first subjected to ultrafiltration through an ultrafiltration diaphragm, whose cut-off zone is between
10.000 og 100.000, ved at det tilsettes minst 1 volumdel vann til vørteren og ved å la minst 1 volumdel væske pr. volum vørter som er underkastet ultrafiltreringen, filtrere 10,000 and 100,000, by adding at least 1 part by volume of water to the wort and by adding at least 1 part by volume of liquid per volume of worts that have been subjected to the ultrafiltration, filter
gjennom og through and
b) deretter oppvarmes den rest som ble oppnådd i trinn a), ved en temperatur på minst 60°C. b) then the residue obtained in step a) is heated at a temperature of at least 60°C.
De forurensninger som har gått gjennom diafragmaet kan kastes. The contaminants that have passed through the diaphragm can be discarded.
I den ultrafiltrering som er beskrevet ovenfor strømmer vann gjennom diafragmaet med forurensningene, slik at den oppnådde resten kan oppvise en større polysakkaridkonsentrasjon enn vørteren før behandling. Dette er imidlertid ikke et forbehold med oppfinnelsen, og vann, fortrinnsvis saltvann, tilsettes for å holde f.eks. den samme polysakkaridkonsentrasjon i vørteren som før behandlingen. En foretrukken utførelsesform består i å følge ultrafiltreringen med progressiv tilsetning til vørteren av minst 1 volumdel vann eller saltvann (f.eks. med 1-100 g/l, uttrykt i NaCl, av salter av alkalimetaller), f.eks. 1-10 volumdeler, fortrinnsvis 5-10 volumdeler, pr. volumdel behandlet vørter, mens minst 1 volumdel vann, f.eks. 1-10 volumdeler, og fortrinnsvis et volum som er mer eller mindre lik volumet til den tilsatte væsken, filtreres gjennom. Dette kan oppnås kontinuerlig eller diskontinuerlig ved å gå gjennom flere ultra-filtreringsceller og/eller tilbakeføre løsningen i den samme cellen flere ganger. Denne behandlingstypen kalles vanligvis diafiltrering. In the ultrafiltration described above, water flows through the diaphragm with the contaminants, so that the obtained residue can exhibit a greater polysaccharide concentration than the wort before treatment. However, this is not a caveat with the invention, and water, preferably salt water, is added to keep e.g. the same polysaccharide concentration in the wort as before the treatment. A preferred embodiment consists in following the ultrafiltration with progressive addition to the wort of at least 1 part by volume of water or salt water (e.g. with 1-100 g/l, expressed in NaCl, of salts of alkali metals), e.g. 1-10 parts by volume, preferably 5-10 parts by volume, per part by volume treated wort, while at least 1 part by volume water, e.g. 1-10 parts by volume, and preferably a volume more or less equal to the volume of the added liquid, is filtered through. This can be achieved continuously or discontinuously by passing through several ultra-filtration cells and/or returning the solution in the same cell several times. This type of treatment is usually called diafiltration.
Etter nevnte diafiltrering kan fremgangsmåten kompletteres ytterligere med en vanlig konsentrering ved hjelp av hvilke som helst midler, inkludert konsentreringsultrafiltrering. After said diafiltration, the method can be further supplemented with a normal concentration using any means, including concentration ultrafiltration.
De foretrukne utførelsesbetingelsene er beskrevet neden-for: Under ultrafiltreringen varierer polymerkonsentrasjonen i vørteren fordelaktig fra 5 til 100 g/l, fortrinnsvis 15-30 g/l. Lavere konsentrasjoner, f.eks. minst 100 ppm, er også egnet. Valget av diafragma er ikke avgjørende, og gode resultater er f.eks. oppnådd med celluloseacetat-, celluloseeter-, polyamid-, polyolefin-, polystyren- og sulfonert polystyren-diafragma, siden oppfinnelsen ikke er begrenset til bruken av et bestemt diafragma. The preferred performance conditions are described below: During the ultrafiltration, the polymer concentration in the wort varies advantageously from 5 to 100 g/l, preferably 15-30 g/l. Lower concentrations, e.g. at least 100 ppm is also suitable. The choice of diaphragm is not decisive, and good results are e.g. achieved with cellulose acetate, cellulose ether, polyamide, polyolefin, polystyrene and sulfonated polystyrene diaphragms, since the invention is not limited to the use of a particular diaphragm.
Den vørter som er underkastet ultrafiltrering omfatter fordelaktig 1-100 g/l, fortrinnsvis 5-50 g/l, uttrykt i NaCl, av et eller flere salter av alkalimetaller. The wort which is subjected to ultrafiltration advantageously comprises 1-100 g/l, preferably 5-50 g/l, expressed in NaCl, of one or more salts of alkali metals.
Vørteren kan ha vært utsatt for en standard prefiltrering, f.eks. for å eliminere cellerester som er større enn 20 mikrometer, fortrinnsvis slike som er større enn 1 mikrometer. The wort may have been subjected to a standard pre-filtration, e.g. to eliminate cell debris larger than 20 micrometers, preferably those larger than 1 micrometer.
Under ultrafiltrering er pH fordelaktig mellom 4 og 11, During ultrafiltration, the pH is advantageously between 4 and 11,
og temperaturen varierer f.eks. fra 10 til 50°C, fortrinnsvis 20-40°C. and the temperature varies e.g. from 10 to 50°C, preferably 20-40°C.
Under oppvarmingen kan polymerkonsentrasjonen velges innenfor grenser som varierer fra 5 til 100 g/l, fortrinnsvis 15-30 g/l. Slik det er nevnt ovenfor, er også lavere konsentrasjoner egnet. During the heating, the polymer concentration can be selected within limits varying from 5 to 100 g/l, preferably 15-30 g/l. As mentioned above, lower concentrations are also suitable.
Den optimale oppvarmingsvarigheten avhenger av det ønskede resultatet og av polysakkaridets natur. Den varierer oftest fra 1 til 60 minutter, fortrinnsvis 5-15 minutter, for xantan, og fra 1 til 24 timer for skleroglucan og skizofyllan. The optimal heating duration depends on the desired result and on the nature of the polysaccharide. It most often varies from 1 to 60 minutes, preferably 5-15 minutes, for xanthan, and from 1 to 24 hours for scleroglucan and schizophyllan.
Følgende sammenlikningseksempler illustrerer fremgangsmåten: The following comparison examples illustrate the procedure:
Eksempel 1 Example 1
En rå fermenteringsvørter av Xanthomonas Campestris er fortynnet for å bringe dens konsentrasjon til 380 vektdeler pr. million. Dens alkalimetallkonsentrasjon, uttrykt i NaCl, er bragt til 5 g/l ved tilsetning av natriumklorid. A raw fermentation wort of Xanthomonas Campestris is diluted to bring its concentration to 380 parts by weight. million. Its alkali metal concentration, expressed in NaCl, is brought to 5 g/l by the addition of sodium chloride.
Tre prøver er tatt av den.oppnådde løsningen (viskositet ved 30°C: 2,IO"<3> Pa.s"<1>, konsentrasjon: 380 vektdeler pr. million). Three samples are taken of the solution obtained (viscosity at 30°C: 2.10"<3> Pa.s"<1>, concentration: 380 parts by weight per million).
Prøve nr. 1 er bragt opp til 110°C i 10 minutter, så avkjølt til 30°C og underkastet filtrerbarhetstesten. Sample No. 1 is brought up to 110°C for 10 minutes, then cooled to 30°C and subjected to the filterability test.
Prøve nr. 2, ifølge oppfinnelsen, er ultrafiltrert ved 30°C ved å bringes i kontakt med et celluloseacetatdiafragma med en avskjæringssone på 20.000. 5 volumer væske pr. volum av prøve nr. 2 får filtrere gjennom, mens den filtrerte væsken progres-sivt erstattes med samme volum av en vandig løsning med 5 g/l NaCl. Den oppnådde rest er utsatt for den samme oppvarming og avkjøling som prøve nr. 1. Den utsettes så for filtrerbarhetstesten. Sample No. 2, according to the invention, is ultrafiltered at 30°C by contacting a cellulose acetate diaphragm with a cut-off zone of 20,000. 5 volumes of liquid per volume of sample no. 2 is allowed to filter through, while the filtered liquid is progressively replaced with the same volume of an aqueous solution with 5 g/l NaCl. The residue obtained is subjected to the same heating and cooling as sample No. 1. It is then subjected to the filterability test.
Prøve nr. 3 er først oppvarmet og avkjølt som prøve nr. 1, så ultrafiltrert ved 30°C som prøve nr. 2, og så utsatt for f i1trerbarhetstesten. Sample No. 3 is first heated and cooled like sample No. 1, then ultrafiltered at 30°C like sample No. 2, and then subjected to the filterability test.
Etter de behandlingene som er beskrevet ovenfor, oppviste både løsning nr. 1 og 3 en viskositet på 7,10"<3> Pa.s-<1> ved 30°C, og løsning nr. 2 hadde en viskositet på 7,7 .10~<3> Pa.s"<1> ved 30°C. After the treatments described above, both solutions No. 1 and 3 exhibited a viscosity of 7.10"<3> Pa.s-<1> at 30°C, and solution No. 2 had a viscosity of 7.7 .10~<3> Pa.s"<1> at 30°C.
Filtrerbarhetstesten besto i injisering, under lave skjær-hastighetsbetingelser ("Y = 5 s"<1>) og ved 3 0°C, av polymer-løsningen gjennom tre Millipore-filtre med en gjennomsnittlig porediameter på 5 /xm og bestemmelse av mobilitetsreduksjonen (R) 1 filtrene ifølge det filtrerte volumet (V). The filterability test consisted in injecting, under low shear rate conditions ("Y = 5 s"<1>) and at 30°C, the polymer solution through three Millipore filters with an average pore diameter of 5 µm and determining the mobility reduction ( R) 1 the filters according to the filtered volume (V).
Kurver henholdsvis Ia, Ila og Illa tilsvarer prøver nr. 1, Curves Ia, Ila and Illa respectively correspond to samples no. 1,
2 og 3. 2 and 3.
Mobilitetsreduksjonen er forholdet A P polymer /A P vann, hvor A P polymer er trykkfallet for polysakkaridløsningen, og A P vann er trykkfallet for den salte vandige fasen uten polysakkarid. The mobility reduction is the ratio A P polymer /A P water, where A P polymer is the pressure drop for the polysaccharide solution, and A P water is the pressure drop for the salty aqueous phase without polysaccharide.
Eksempel 2 Example 2
Eksempel 1 ble gjentatt med løsninger som var identiske med løsningene nr. 1 og nr. 2, bortsett fra at saltkonsentra-sjonen, uttrykt i NaCl, var 100 g/l. Example 1 was repeated with solutions identical to solutions No. 1 and No. 2, except that the salt concentration, expressed in NaCl, was 100 g/l.
Etter behandlingen oppviste de oppnådde løsningene (henholdsvis Ib og Ilb) en viskositet på 8,5 -IO"<3> Pa.s-<1>. After the treatment, the solutions obtained (Ib and Ilb, respectively) showed a viscosity of 8.5 -10"<3> Pa.s-<1>.
Begge løsninger er underkastet filtrerbarhetstesten (fig. 2). Bare løsning nr. 2, som er underkastet behandlingen ifølge oppfinnelsen, viste ikke-tetting. Both solutions are subjected to the filterability test (Fig. 2). Only solution No. 2, which has been subjected to the treatment according to the invention, showed non-sealing.
Eksempel 3 Example 3
Testen gjaldt en rå skleroglukanvørter. En rå vørter fra Sclerotium Rolfsii-fermenterin<q>. hvorfra sopper større enn 20 mikrometer er eliminert ved filtrering, er fortynnet inntil en konsentrasjon på 600 vektdeler pr. million i en saltløsning med en natriumkloridkonsentrasjon på 20 g/l. The test concerned a raw scleroglucan wort. A raw wort from Sclerotium Rolfsii fermenterin<q>. from which fungi larger than 20 micrometers have been eliminated by filtration, is diluted to a concentration of 600 parts by weight per million in a salt solution with a sodium chloride concentration of 20 g/l.
Den oppnådde løsning (løsning Ic) er så ultrafiltrert ved 30°C ved kontakt med et celluloseacetatdiafragma med en avskjæringssone på 20.000, som vist i eksempel 1. Resten (Ille) er så oppvarmet til 90°C under atmosfærisk trykk i 48 timer. Etter avkjøling til 30°C er den oppnådde løsning (lic) underkastet den samme filtrerbarhetstest som beskrevet i eksempel 1. Fluiditets-reduksjonen gjennom 3 Millipore-filtre med en gjennomsnittlig porediameter på 5 /xm er målt ifølge det filtrerte volumet. The solution obtained (solution Ic) is then ultrafiltered at 30°C in contact with a cellulose acetate diaphragm with a cut-off zone of 20,000, as shown in example 1. The residue (Ille) is then heated to 90°C under atmospheric pressure for 48 hours. After cooling to 30°C, the obtained solution (lic) is subjected to the same filterability test as described in example 1. The fluidity reduction through 3 Millipore filters with an average pore diameter of 5 µm is measured according to the filtered volume.
Fig. 3 viser de tester som tilsvarer en ikke-behandlet løsning (Ic), en løsning som bare er ultrafiltrert (Ille) og en løsning (lic) som er behandlet ifølge oppfinnelsen (ultrafiltrering og oppvarming). Det kan sees at de første to løsningene meget raskt tetter filtrene og at bare løsningen som er behandlet ifølge oppfinnelsen viser en konstant R, dvs. en utmerket filtrerbarhet. Fig. 3 shows the tests corresponding to an untreated solution (Ic), a solution that has only been ultrafiltered (Ille) and a solution (lic) that has been treated according to the invention (ultrafiltration and heating). It can be seen that the first two solutions very quickly clog the filters and that only the solution treated according to the invention shows a constant R, i.e. an excellent filterability.
Eksempel 4 Example 4
En Xanthomonas Campestr is-fermenteringsvørter med en konsentrasjon på 15 g/l er oppvarmet ved 110°C i 10 minutter og så avkjølt til 30°C. Vørteren (prøve nr. 1) er så fortynnet med en konsentrasjon på 550 vektdeler pr. million i en vandig løsning av 5 g/l NaCl. Denne løsningen hadde en viskositet på 9,7 .IO"<3> Pa.s"<1.>A Xanthomonas Campestr ice fermentation wort with a concentration of 15 g/l is heated at 110°C for 10 minutes and then cooled to 30°C. The wort (sample no. 1) is then diluted to a concentration of 550 parts by weight. million in an aqueous solution of 5 g/l NaCl. This solution had a viscosity of 9.7 .IO"<3> Pa.s"<1.>
Når det gjelder fremstillingen av prøve nr. 2, er fermen-ter ingsvørteren ultrafiltrert etter tilsetning av en vandig løsning av 5 g/l NaCl, under de samme betingelser som de som er beskrevet for prøve nr. 2 i eksempel 1. Den er så underkastet den samme oppvarming og avkjøling som prøve nr. 1. Vørteren er også fortynnet med en konsentrasjon på 550 vektdeler pr. million i en vandig løsning på 5 g/l NaCl. Viskositeten av denne løsningen var 10,4 -10"<3>Pa.s-<1>. As regards the preparation of sample no. 2, the fermentation wort is ultrafiltered after the addition of an aqueous solution of 5 g/l NaCl, under the same conditions as those described for sample no. 2 in example 1. It is then subjected to the same heating and cooling as sample No. 1. The wort is also diluted with a concentration of 550 parts by weight. million in an aqueous solution of 5 g/l NaCl. The viscosity of this solution was 10.4 -10"<3>Pa.s-<1>.
De to løsningene som er fremstilt med prøve nr. 1 og 2 (henholdsvis Id og Ild) er underkastet filtrerbarhetstesten (fig. 4). Den vørter som var underkastet behandlingen ifølge oppfinnelsen muliggjorde fremstilling av en ikke-tettende løsning, noe som ikke var tilfellet med den ikke-behandlede vørteren. The two solutions prepared with sample no. 1 and 2 (respectively Id and Ild) have been subjected to the filterability test (fig. 4). The wort subjected to the treatment according to the invention enabled the production of a non-clogging solution, which was not the case with the untreated wort.
Claims (11)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8714582A FR2621926B1 (en) | 1987-10-20 | 1987-10-20 | PROCESS FOR THE TREATMENT OF A FERMENTATION MUST CONTAINING A POLYSACCHARIDE FOR THE PURPOSE OF INCREASING THE FILTRABILITY AND USE OF THIS ASSIST IN ASSISTED OIL RECOVERY |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| NO884635D0 NO884635D0 (en) | 1988-10-18 |
| NO884635L NO884635L (en) | 1989-04-21 |
| NO173145B true NO173145B (en) | 1993-07-26 |
| NO173145C NO173145C (en) | 1993-11-03 |
Family
ID=9356060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO884635A NO173145C (en) | 1987-10-20 | 1988-10-18 | PROCEDURE FOR TREATMENT OF FEEDS |
Country Status (8)
| Country | Link |
|---|---|
| JP (1) | JP2683813B2 (en) |
| CN (1) | CN1029240C (en) |
| CA (1) | CA1331358C (en) |
| DE (1) | DE3835771A1 (en) |
| FR (1) | FR2621926B1 (en) |
| GB (1) | GB2211195B (en) |
| IN (1) | IN172065B (en) |
| NO (1) | NO173145C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2639645B1 (en) * | 1988-11-25 | 1992-05-29 | Elf Aquitaine | CONCENTRATED LIQUID SOLUTIONS OF POLYSACCHARIDES |
| US5786343A (en) * | 1997-03-05 | 1998-07-28 | Immudyne, Inc. | Phagocytosis activator compositions and their use |
| US8252266B2 (en) * | 2010-08-09 | 2012-08-28 | Nalco Company | Recovery of alumina trihydrate during the bayer process using scleroglucan |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1070629A (en) * | 1975-11-10 | 1980-01-29 | Allen I. Laskin | Process for modifying biopolymers |
| NO158658C (en) * | 1981-07-01 | 1988-10-19 | Pfizer | PROCEDURE FOR CONCENTRATING Aqueous PSEUDOPLASTIC SOLUTIONS. |
| FR2551087B1 (en) * | 1983-08-30 | 1986-03-21 | Rhone Poulenc Spec Chim | PROCESS FOR TREATING POLYSACCHARIDE SOLUTION AND USE THEREOF |
| FR2551070B1 (en) * | 1983-08-30 | 1986-09-26 | Rhone Poulenc Spec Chim | PROCESS FOR TREATING POLYSACCHARIDE SOLUTION AND USE THEREOF |
| JPS6062996A (en) * | 1983-09-17 | 1985-04-11 | Kohjin Co Ltd | Preparation of xanthan gum |
-
1987
- 1987-10-20 FR FR8714582A patent/FR2621926B1/en not_active Expired - Fee Related
-
1988
- 1988-10-11 IN IN710/MAS/88A patent/IN172065B/en unknown
- 1988-10-18 NO NO884635A patent/NO173145C/en not_active IP Right Cessation
- 1988-10-20 CA CA000580767A patent/CA1331358C/en not_active Expired - Fee Related
- 1988-10-20 GB GB8824537A patent/GB2211195B/en not_active Expired - Fee Related
- 1988-10-20 DE DE3835771A patent/DE3835771A1/en not_active Ceased
- 1988-10-20 JP JP63265928A patent/JP2683813B2/en not_active Expired - Fee Related
- 1988-10-20 CN CN88107257A patent/CN1029240C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| NO884635D0 (en) | 1988-10-18 |
| CA1331358C (en) | 1994-08-09 |
| CN1029240C (en) | 1995-07-05 |
| JP2683813B2 (en) | 1997-12-03 |
| DE3835771A1 (en) | 1989-05-03 |
| JPH01153098A (en) | 1989-06-15 |
| NO884635L (en) | 1989-04-21 |
| GB8824537D0 (en) | 1988-11-23 |
| GB2211195B (en) | 1991-02-13 |
| FR2621926A1 (en) | 1989-04-21 |
| FR2621926B1 (en) | 1991-06-21 |
| IN172065B (en) | 1993-03-27 |
| GB2211195A (en) | 1989-06-28 |
| CN1032674A (en) | 1989-05-03 |
| NO173145C (en) | 1993-11-03 |
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