NL2031136B1 - Set of kompetitive allele specific pcr (kasp)-based core single nucleotide polymorphism (snp) markers for eggplant and use thereof - Google Patents
Set of kompetitive allele specific pcr (kasp)-based core single nucleotide polymorphism (snp) markers for eggplant and use thereof Download PDFInfo
- Publication number
- NL2031136B1 NL2031136B1 NL2031136A NL2031136A NL2031136B1 NL 2031136 B1 NL2031136 B1 NL 2031136B1 NL 2031136 A NL2031136 A NL 2031136A NL 2031136 A NL2031136 A NL 2031136A NL 2031136 B1 NL2031136 B1 NL 2031136B1
- Authority
- NL
- Netherlands
- Prior art keywords
- seq
- eggplant
- kasp
- markers
- snp
- Prior art date
Links
- 244000061458 Solanum melongena Species 0.000 title claims abstract description 50
- 235000002597 Solanum melongena Nutrition 0.000 title claims abstract description 49
- 239000002773 nucleotide Substances 0.000 title claims abstract description 6
- 125000003729 nucleotide group Chemical group 0.000 title claims abstract description 4
- 238000007844 allele-specific PCR Methods 0.000 title claims description 5
- 238000004458 analytical method Methods 0.000 claims abstract description 8
- 230000002068 genetic effect Effects 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims description 13
- 239000000523 sample Substances 0.000 claims description 6
- 101000690100 Homo sapiens U1 small nuclear ribonucleoprotein 70 kDa Proteins 0.000 claims description 5
- 101100029173 Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) SNP2 gene Proteins 0.000 claims description 5
- 101100236128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LSM2 gene Proteins 0.000 claims description 5
- 101100094821 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SMX2 gene Proteins 0.000 claims description 5
- 102100024121 U1 small nuclear ribonucleoprotein 70 kDa Human genes 0.000 claims description 5
- 238000010276 construction Methods 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 239000007791 liquid phase Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000002860 competitive effect Effects 0.000 claims 1
- 210000000349 chromosome Anatomy 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 4
- 238000012790 confirmation Methods 0.000 abstract description 2
- 238000013094 purity test Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 16
- 238000000034 method Methods 0.000 description 9
- 230000003321 amplification Effects 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012214 genetic breeding Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical class [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 241001386813 Kraken Species 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000007614 genetic variation Effects 0.000 description 2
- 238000011068 loading method Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 241000511343 Chondrostoma nasus Species 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/08—Fruits
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
- A01H6/826—Solanum melongena [eggplant]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (6)
1. KASP-primers voor het amplificeren van SNP-merkers, waarbij de primers een verzameling van kern-enkelenucleotidepolymorfisme- (SNP) merkers op basis van kompetitieve allelspecifieke PCR die geisoleerd zijn uit een aubergine omvatten, waarbij de verzameling 20 kern-SNP-merkers als volgt omvat: SNP- Chromos | Allelvan | Allelvan | SNP- Chromios | Allelvan | Allelvan waarbij de 20 kern-SNP-merkers SNP1, SNP2, SNP3, SNP4, SNP5, SNP6, SNP7, SNPS, SNP9, SNPI0, SNP11, SNP12, SNPI3, SNP14, SNP15, SNP16, SNP17, SNPI8, SNP19, SNP20 respectievelijk door de volgende primers geamplificeerd zijn: Seq ID Nr. 1-3, Seq ID NR. 4-6, Seq ID NR. 7-9, Seq ID NR. 10-12, Seq ID NR. 13-15, Seq ID NR. 16-18, Seq ID NR. 19-21, Seq ID NR. 22-24, Seq ID NR. 25-27, Seq ID NR. 28-30, Seq ID NR. 31-33, Seq ID NR. 34-36, Seq ID NR. 37-39, Seq ID NR. 40-42, Seq ID NR. 43-45, Seq ID NR. 46-48, Seq ID NR. 49-51, Seq ID NR. 52-54, Seq ID NR. 55-57 en Seq ID NR. 58-60.
2. Gebruik van de KASP-primers volgens conclusie 1 in constructie van een SNP- vingerafdruk van de aubergine.
3. Gebruik van de KASP-primers volgens conclusie | in detectie van een bron van de aubergine.
S12 -
4. Gebruik van de KASP-primers volgens conclusie 1 in analyse van een genetische diversiteit van de aubergine.
5. Gebruik van de KASP-primers volgens conclusie 1 in taxonomie van de aubergine.
6. Gebruik van de KASP-primers volgens conclusie 1 in bereiding van een detectiereagent, een kit, een genoomchip of een vloeibarefasesonde van de aubergine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2031136A NL2031136B1 (en) | 2022-03-02 | 2022-03-02 | Set of kompetitive allele specific pcr (kasp)-based core single nucleotide polymorphism (snp) markers for eggplant and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL2031136A NL2031136B1 (en) | 2022-03-02 | 2022-03-02 | Set of kompetitive allele specific pcr (kasp)-based core single nucleotide polymorphism (snp) markers for eggplant and use thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NL2031136B1 true NL2031136B1 (en) | 2023-09-08 |
Family
ID=82404176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NL2031136A NL2031136B1 (en) | 2022-03-02 | 2022-03-02 | Set of kompetitive allele specific pcr (kasp)-based core single nucleotide polymorphism (snp) markers for eggplant and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| NL (1) | NL2031136B1 (nl) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229928A (zh) * | 2019-06-27 | 2019-09-13 | 江西省农业科学院蔬菜花卉研究所 | 用于茄子种质资源鉴定的分子标记组合及其应用 |
| CN112029890A (zh) * | 2020-09-07 | 2020-12-04 | 北京市农林科学院 | 一种鉴定甜瓜种质真实性的snp位点引物组合及应用 |
| CN112094939A (zh) * | 2020-09-29 | 2020-12-18 | 北京市农林科学院 | 一种鉴定茄子品种真实性的方法及其使用的引物组合 |
| CN112251533A (zh) * | 2020-11-09 | 2021-01-22 | 浙江省农业科学院 | 与茄子果实长度主效qtl位点紧密连锁的snp分子标记及应用 |
| CN112813191A (zh) * | 2021-03-30 | 2021-05-18 | 天津市农业科学院 | 一种与茄子萼片覆盖下果皮颜色相关的分子标记及应用 |
| CN113943731A (zh) * | 2021-11-30 | 2022-01-18 | 中国农业科学院蔬菜花卉研究所 | 一种利用kasp分子标记鉴定茄子显性单性结实基因的引物对及其应用 |
-
2022
- 2022-03-02 NL NL2031136A patent/NL2031136B1/en active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110229928A (zh) * | 2019-06-27 | 2019-09-13 | 江西省农业科学院蔬菜花卉研究所 | 用于茄子种质资源鉴定的分子标记组合及其应用 |
| CN112029890A (zh) * | 2020-09-07 | 2020-12-04 | 北京市农林科学院 | 一种鉴定甜瓜种质真实性的snp位点引物组合及应用 |
| CN112094939A (zh) * | 2020-09-29 | 2020-12-18 | 北京市农林科学院 | 一种鉴定茄子品种真实性的方法及其使用的引物组合 |
| CN112251533A (zh) * | 2020-11-09 | 2021-01-22 | 浙江省农业科学院 | 与茄子果实长度主效qtl位点紧密连锁的snp分子标记及应用 |
| CN112813191A (zh) * | 2021-03-30 | 2021-05-18 | 天津市农业科学院 | 一种与茄子萼片覆盖下果皮颜色相关的分子标记及应用 |
| CN113943731A (zh) * | 2021-11-30 | 2022-01-18 | 中国农业科学院蔬菜花卉研究所 | 一种利用kasp分子标记鉴定茄子显性单性结实基因的引物对及其应用 |
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