MXPA96004278A - Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal. - Google Patents

Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal.

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Publication number
MXPA96004278A
MXPA96004278A MXPA96004278A MX9604278A MXPA96004278A MX PA96004278 A MXPA96004278 A MX PA96004278A MX PA96004278 A MXPA96004278 A MX PA96004278A MX 9604278 A MX9604278 A MX 9604278A MX PA96004278 A MXPA96004278 A MX PA96004278A
Authority
MX
Mexico
Prior art keywords
fetal
cells
disease
sought
stem cells
Prior art date
Application number
MXPA96004278A
Other languages
English (en)
Inventor
P Alter Blanche
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of MXPA96004278A publication Critical patent/MXPA96004278A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Los metodos de la presente invencion usan sangre de mujeres embarazadas y amplificacion de celulas fetales para facilitar el diagnostico prenatal sin invadir el espacio fetal. Este metodo es menos invasor y mas simple que la amniocentesis o el muestreo de vellosidad corionica porque solamente involucra extraer sangre de la madre para obtener una muestra de celulas fetales. Si se esta buscando una enfermedad especifica con mutaciones moleculares conocidas, las celulas de la muestra son una fuente de DNA enriquecido para el material fetal. Si la enfermedad involucra a los globulos rojos sanguineos, este metodo proporciona globulos rojos sanguineos fetales para su analisis. Si la enfermedad involucra globulos blancos sanguineos, se pueden usar distintas citocinas para producir selectivamente leucocitos fetales. Si se esta buscando un patron de cromosomas anormales sin un riesgo especifico de diagnostico (por ejemplo, por edad avanzada de la madre), entonces el metodo de cultivo proporciona celulas en division de origen fetal. La figura mas representativa de la invencion es la numero 6.
MXPA96004278A 1994-03-25 1995-03-24 Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal. MXPA96004278A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/218,290 US5580724A (en) 1994-03-25 1994-03-25 Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis
PCT/US1995/003659 WO1995026507A1 (en) 1994-03-25 1995-03-24 Differential expansion of fetal stem cells in the maternal circulation for use in prenatal genetic analysis

Publications (1)

Publication Number Publication Date
MXPA96004278A true MXPA96004278A (es) 2002-04-19

Family

ID=22814517

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA96004278A MXPA96004278A (es) 1994-03-25 1995-03-24 Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal.

Country Status (9)

Country Link
US (1) US5580724A (es)
EP (1) EP0752104B1 (es)
JP (1) JP2996513B2 (es)
AU (1) AU684935B2 (es)
CA (1) CA2186366C (es)
DE (1) DE69501326T2 (es)
ES (1) ES2111401T3 (es)
MX (1) MXPA96004278A (es)
WO (1) WO1995026507A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731156A (en) * 1996-10-21 1998-03-24 Applied Imaging, Inc. Use of anti-embryonic hemoglobin antibodies to identify fetal cells
US6617158B1 (en) * 1999-08-19 2003-09-09 New England Medical Center Method for stimulating the production of fetal hemoglobin producing erythroid cells
US7354730B2 (en) * 2002-01-29 2008-04-08 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
US7666615B2 (en) * 2001-01-29 2010-02-23 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
US7989178B2 (en) * 2001-01-29 2011-08-02 Hemogenix, Inc. Colony assay miniaturization with enumeration output
EP1364197B1 (en) * 2001-01-29 2010-04-14 Ivan N. Rich ATP-based assay for the determination of the proliferation status of hematopoietic stem and progenitor cells
FR2824144B1 (fr) * 2001-04-30 2004-09-17 Metagenex S A R L Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel
JP4330995B2 (ja) * 2001-11-15 2009-09-16 チルドレンズ メディカル センター コーポレーション 絨毛膜絨毛、羊水、および胎盤からの胎児性幹細胞を単離、増殖、および分化させる方法、ならびにその治療的使用方法
ATE324903T1 (de) 2003-02-18 2006-06-15 Clinique La Prairie Res Sa Zusammensetzungen enthaltend fötales hämoglobin und bakterielles endotoxin und fakultativ zusätzliche fötale leberkomponenten
WO2005046596A2 (en) * 2003-11-04 2005-05-26 University Of Maryland Baltimore Stem cell culture medium and method of using said medium and the cells
WO2005123779A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The University Of Illinois Antibodies binding to cd34+/cd36+ fetal but not to adult cells
KR20200123283A (ko) 2005-12-29 2020-10-28 안트로제네시스 코포레이션 태반 줄기 세포 집단
AU2007311126A1 (en) * 2006-10-16 2008-04-24 Celula Inc. Methods and compositions for differential expansion of fetal cells in maternal blood and their use
AU2011352036A1 (en) 2010-12-31 2013-07-18 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
CA2837871C (en) 2011-06-01 2021-12-07 Anthrogenesis Corporation Treatment of pain using placental stem cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2081203A1 (en) * 1990-04-23 1991-10-24 Ronald J. Berenson Method for enriching fetal cells from maternal blood
EP0610359A1 (en) * 1991-10-23 1994-08-17 Cellpro Incorporated A method for enriching fetal progenitor cells from maternal blood
ES2180539T3 (es) * 1991-10-23 2003-02-16 Nexell Therapeutics Inc Metodos para expandir selectivamente celulas madre.
WO1993018136A1 (en) * 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
JPH08511935A (ja) * 1993-01-27 1996-12-17 ヘモソル インコーポレーテッド 選択的細胞増殖

Also Published As

Publication number Publication date
DE69501326D1 (de) 1998-02-05
ES2111401T3 (es) 1998-03-01
JP2996513B2 (ja) 2000-01-11
EP0752104A1 (en) 1997-01-08
DE69501326T2 (de) 1998-04-16
EP0752104B1 (en) 1997-12-29
CA2186366C (en) 2008-07-08
WO1995026507A1 (en) 1995-10-05
JPH09509328A (ja) 1997-09-22
AU684935B2 (en) 1998-01-08
CA2186366A1 (en) 1995-10-05
US5580724A (en) 1996-12-03
AU2229895A (en) 1995-10-17

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