MXPA06015194A - Method of obtaining cyanogenic glycosides and related glycosides, as well as genin and sapogenin derivatives of plants of the sapodilla family, for the preparation of cosmetics and dermatological compositions - Google Patents

Abstract

The invention relates to a method of obtaining extracts of genins and sapogenins, comprising the enzymatic hydrolysis of derivatives of seeds of the sapodilla family, genera Calocarpum, Chrysophyllum and Lucuma. According to the invention, the enzyme that catalyses the hydrolysis is aâ-exo-glucosidase of plant or microbial origin. The seed derivatives (grains free from pericarp) can be cyanogenic glycoside, lucumin and related glycosides. The invention also relates to a method of producing said glycosides from seeds and to the use thereof in cosmetic and dermatological compositions as well as in the production of genins and sapogenins. The genin extracts thus obtained are used in topical compositions in order to promote the opening of pores and epithelial penetration. The sapogenin extracts thus obtained are used in topical compositions as an astringent and exfoliant in order to promote skin renewal and hair growth, by slowing down hair loss, and in order to combat cutaneous ageing, owing to the bacteriostatic and fungistatic activity thereof.

Description

OBTAINING THE CYANOGENIC AND RELATED GLYCOSIDES, THE MOST GENE AND SAPOGENIC DERIVATIVES OF PLANTS FROM THE SAPOTÁCEA FAMILY FOR THE PREPARATION OF COSMETICS AND DERMATOLOGICAL COMPOSITIONS.

BACKGROUND This invention is a continuation of another related patent application by the same author with the title: OBTAINING THE TOTAL FIXED SEED LIPIDS FROM THE SAPOTÁCEA FAMILY FOR THE PREPARATION OF COSMETICS AND PHARMACEUTICAL DERMATOLOGICAL COMPOSITIONS (ref 68). This invention relates to obtaining the cyanogenic glycoside, lucumin and related structurally related glycosides, lucuminic acid and lucuminamide and their hydrolytic transformations to alpha hydroxylated derivatives, the former with hydroxynitrilic characteristic initially, such as mandelonitrile; subsequently transformed into benzaldehyde, as finally obtained; the other two related glycosides are obtained and transformed, the second as carboxylic acid, similar to mandelic acid; and the third in amide of the same acid; both hydroxylated as finally obtained, all achieved and converted the three primarily into a united extracts of genins and sapogenins respectively, which are separated by their physical properties. These are derived by chemical or enzymatic hydrolysis of the structurally related glycosides related to lucumin, major genera of Calocarpum, Chrysophyllum and Lucuma of the Sapotacea family, all of them with biological activity. The biological activities are presented as isolated compounds or in a mixture of glycosidic extractive substances, or of aglyconic hydrolytic extracts (glycosides devoid of the "azucarad" residue) of the above compounds; the two groups of aglyconic derivatives, the genins and sapogenins, isolated and purified with specific chemical and biological characteristics of use in consumer products, as presented in this work and the way of use in cosmetics and dermatological pharmaceutical compositions and in substitute uses, same as exposed here. The sources to obtain the aglyconic hydrolytic derivatives (later only in chemical structure to the glycosides but not necessarily in the production chain), here called genic and sapogenin derivatives, can be several principles or tissues of the trees of the Sapotaceae family. { Calocarpum sapota, C. viride, Lucuma mammosa, L. salicifolia and some other fruit trees of the same known family as "sapotes" included to Chrysop yllu), such as barks, leaves, flowers or sage; but as a renewable resource it turns out to be the tissue of the seeds used in various ways, from the raw material crushed (milled), or what leaves the seed of solid remnant after lipid expression or what is left as solid residue the extraction of Lipids (to obtain the oils) or special circumstances are also possible to obtain the related glycosidic extracts but not in a dependent manner (forcing), it only needs the seeds to contain them as it is naturally, without suffering contact with polar liquids that extract them and so proceed to the aglyconical hydrolysis starting from residual products or from the raw material, plainly ground or crushed from the seeds. Of the raw materials mentioned in the previous paragraph, (sources of the aglyconic derivatives), they are mainly of the seeds in the raw state, plainly ground, crushed or cut into small pieces that are used or of some preparation of them (seeds) , to obtain the aglycone hydrolytic derivatives (of the glycosides structurally related to the cyanoglycoside) of the related genera. It can also be deviated in the previous obtaining and achieve in an isolated or semipurified extract the direct hydrolytic (enzymatic) substrates, the related glycosides, without bagasse material, and then continue with the related hydrolysis or it is possible to obtain the related glycosides and be used in the manufacture of manufactured products (cosmetics or dermatological compositions) in the form of an extract or in isolation. The glycosides related to the cyanoglycoside are preferably used as "enzymatic" substrates which undergo splitting (being able to be chemical with organic or inorganic acids), to obtain the aglyconic hydrolytic derivatives the genins and sapogenins initially mentioned, plus this form of derivation in order to reach the aglycones, it does not have a forced dependency on the production chain. Practically in this work, the genins and sapogenins are obtained directly from the various preparations of the seeds mentioned above without stopping in a first stage to extract the glycosides predecessors structurally to the aglyconic derivatives but contained in the preparations in the matter of the seeds, practicing the hydrolysis followed and thus obtain the aglycones; or it is also possible to deviate in the course of transformation, aglyconic hydrolytics of the seeds, to instead obtain the related glycosidic compounds or extract (to the cyanoglycoside) from the seeds and these are used in the manufacture of the cosmetic and dermatological compositions. These glycosides are obtained as a glycosidic extract related to the cyanoglycoside and the glycosides can be isolated and used in such formulations; or it is possible to resume the obtaining of the aglyconic hydrolytic derivatives, but instead of using some preparation of the seeds the related glycosidic preparation is used, practicing the hydrolysis in the form of the whole glycosidic extract, formed by the three related glycosides or practicing them hydrolysis in isolated form. The techniques of extraction and / or isolation of the cyanogenic glycosides and related ones for the obtaining of the aglyconic hydrolytic derivatives are additionally more expensive than the technique that starts from the different preparations of the seeds and that by direct treatment of these products or preparations of the seeds containing to the related hydrolytic glycosidic substrates, the aglyconic derivatives are reached without being diverted to be isolated and the related glycosidic compounds, structurally related, previously mentioned, to the aglyconic hydrolytic derivatives but both coming from the same previous material sources, prepared directly from the seeds of the related genera but by different ways to obtain them. The preference for obtaining the glycosides related to the cyanoglycoside is received by the remnants of the degreased seeds (see residual solid product of lipid extraction or degreased flour in reference 68 at the end of this work); this is because some or all of the lipids present in the seeds that interfere in the most expeditious purification of these are eliminated and evaded. Also the preference to obtain the aglyconic derivatives of the above compounds, the genetic and sapogeninic extracts, are received by the remnants, residual solids of the seeds, lipid expression and degreased by the lipid extraction, without being limited the raw materials of that part but it is of more profitability by the previous use of fixed lipid that are recovered from the seeds. The biologically active genic and sapogeninic extracts are obtained from lucumin, cyanogenic glycoside and related glycosides, lucuminic acid and lucuminamide; "secondary vegetable metabolites", of the related genera that mainly transformed biochemically with aggregated enzymes of different genera (exogenous), suitable and particular for their ability to carry out the reactions of aglycone hydrolytic cleavage, a process that achieves the detoxification of lucumin in the genin extract mixed with the extract of the sapogenins that is achieved equal by a similar transformation of the remaining related glycosides. The lucuminic acid and lucuminamide of the "sapotes" are achieved by the previous transformation in the alpha hydroxylated derivatives, linked to a benzyl ring (phenyl); one is obtained as a carboxylic acid such as madderic acid, and the other as an amide of the same previous acid derivative, as obtained in the sapogenin extract, and on the other hand obtaining the benzaldehyde derivative as mentioned above with chemical characteristics similar to the others two aglycones and that is obtained in the genic extract. Although both extracts, genic and sapogeninic, are obtained united in a mixture of extracts, that is to have started from some preparation of the seeds, (with the whole natural content of related glycosides) or having split the related glycosidic whole extract related to the cyanoglycoside Obtaining the residual solid product of the lipid expression of the preceding patent application (ref 68) here also referred to as "the pork rind of expression", residual solid product (remnant) in the obtaining of the total fixed lipids and the obtaining of the solid product (residual) of the lipid extraction, like here named as "the defatted flour" as residual solid product of the lipid extraction (ref. 68), both as plant remnants of the seeds of the "sapotes" that remain as a residue of those processes for obtaining the lipids (covered by the previous patent application [ref 68]), and that serve as products and materials premiums, respectively, for that and for this next process of this application, to obtain the cyanogenic and related glycosides and their initially referred genino and sapogeninic derivatives, which are motives of this application and of the previous related patent application in process by the same author, made of materials and products that are also constituted as raw materials and elaborated for this invention and the p recedent respectively.

The technique of choice for obtaining the genetic and sapogeninic extracts is the enzymatic hydrolysis and preferably the vegetal tissue of the substrate is selected from the raw seeds ground or prepared as solid residues from the obtaining of the lipids, which are treated and prepared by the techniques described in the related application of patent by the same author (ref 68), degreasing for obtaining the total fixed lipids and the solid remnants of these processes of Calocarpum, Chrysophyllum and Lucuma, without being restrictive for this obtaining the substrate raw materials referred to but of more profitability in its yield in the integral use of the seeds as it was said previously, being able to be the same weave of the fresh or dry raw ground seed, or different more purified preparations of the seeds as mentioned above those that are used. The raw material in particular of the seeds that are used mainly in the present and previous work are the seeds (seed devoid of the endocarp) crushed and can be used raw as is. If the product you want to reach are the aglyconic hydrolytic derivatives, you can start from any raw material from the seeds mentioned above, which may be the raw seeds or some preparation of the same seeds removed from the lipid content as by the expression processes lipid or oil extraction or both processes (ref 68), without having undergone any extractive or washing procedure with polar solvents (such as water). As it was said previously to arrive at the aglyconic hydrolytic derivatives it is also possible to start from some glycosidic extract related to the whole cyanoglycoside or further purified, however this technique to reach these derivatives is relatively more expensive and without a strict dependence, that is split of these glycosidic resources extracted and isolated or purified from the original seeds. If the glycosidic extract related to the cyanoglycoside or some glycoside of these in isolation is required, it is the reason why it would be useful to deviate in obtaining them. If it is desired to reach the aglycone hydrolytic derivatives more economically and more easily and efficiently, the solid residual products of degreasing (expression or extraction, both lipids) derived from the seeds are equally feasible being isolated between if the material resources or products obtained in the process of agliconic hydrolysis, to the same conditions of simplicity. To derive aglyconic hydrolytic products, it is possible to perform chemical or enzymatic hydrolysis. Preferably the enzymatic hydrolysis practiced on the "degreased flours" and / or the "chicharrón de expresion" of the seeds is used directly (with the related glycosidic content related to the cyanoglycoside) (see expression and lipid extraction [ref 68]). To obtain hydrolysis derivatives chemical or enzymatic, which are to the geninic and sapogeninic extract as a whole of the related "sapotes" it is also possible to use the glycosidic raw material related to cyanoglycoside extracted as described below, which are found in the seeds in a crude form in the same seeds or use the seeds in a degreased form totally or partially or starting from the related glycosides isolated from the aforementioned extract. It is also possible to use the fresh or dried milled seeds without degreasing to obtain the glycosides related to the cyanoglycoside or to the chemical or enzymatic hydrolysis derivatives that are alternate raw material in variants of this invention. The purpose of the present invention is to put these products with structurally related cyanoglycoside principles or their aglycone hydrolytic derivatives, the genins and the sapogenins plus the set of fixed lipids (the latter are the motive of another previous request in connection, related to patent by the same author (ref 68) of the Calocarpum, Chrysophyllum or Lucuma to the public reach in engineering forms through industrial transformations of application, which resolves the technical problems in the uses of raw materials of these medicinal plants and in traditional rustic uses by modern practices in dermatology and cosmetology as well as in other utilities such as substitute food uses, in a satisfactory way and the rational use of resources badly used in crude treatments or as waste vegetable matter, without being used In remote regions of central Mexico and further south, the use of medicinal preparations, cosmetics and food in traditional uses is used plant matter of Calocarpum, Chrysophyllum or Lucuma (ref 5) including the The residues of the seeds after the "extraction" of the oils (which contain the related glycosides) are also reported to be active against painful skin conditions through their application as poultices (ref. 4) . It is reported (ref.4, 15) the bark as astringent, bitter and containing lucumin, the cyanogenic glycoside. Decoctions of the cortex are taken as pectorals. In Costa Rica bark and leaf tees are administered at arteriosclerosis and hypertension. The milky sap is emetic and anthelmintic and has been used topically in the removal of warts and fungal growth of the skin.

Decoctions of the bark of the yellow sapote are also taken as a febrifuge or in skin rashes. The seed is prepared as a remedy for skin ulcers (ref 4). In alternate variants of the present invention, triterpenic, steroidal, and cyanogenic compounds are also found in the seeds as reported by references 18,19,27,67 and 69 such as alpha and beta amyrin, lupeol, lanosterol or lucumin. , free or esterified. These plants have a wide variety of traditional food and medicinal uses of which the main use is the pulp of the fruit for its excellent flavors and traditional recipes high in protein, carbohydrates, fibers, fats, mineral salts, vitamins; especially carotenoid compounds, thiamine, riboflavin, niacin, ascorbic acid (ref. 4, 7, 8,). The plant matter of Calocarpum, Chrysop yllum or Lucuma is also appreciated for its high content of fixed and volatile oils present and which also have traditional uses in the kitchen (ref 4,7,8) and in home remedies for use in the skin and hair (ref.4,8). However, all these preparations of traditional use have not been fully developed due to technical impediments concerning the method of separation and isolation of the active ingredients that give these effects and lead to industrial and consumer processes. • The volatile oils in traditional preparations are purely with an ethereal, aromatic contact effect of the raw vegetable matter (ref. 8) that it is not possible to separate from the plant material in a conventional way and so little is transformed and separated at present as in the way explained in the obtained in the present work. These essential aromas (volatile oils) characteristic of related plants that are not attractive to obtain by conventional techniques to achieve these insoluble flavored materials, genic fraction, and water soluble as the sapogenin fraction that is intimately linked to the structural material of the plant, as they turn out to be the sapogenic glycosidic material. The seed is reported (ref 4) to be a source or substitute for the fragrance "Noyeau" of perfumery and alcoholic beverages, replacing the seeds of bitter almond, peach, peach, apricot, apricot or prisco (ref. 9), the different varieties of the present work being different from the treatments of the previous reference.

In regions where the pulp of the fruit of Calocarpum, Chrysophyllum or Lucuma is eaten widely, which results as a food and vitamin material (ref 4,8), which insensibly also contains active ingredients for the medicinal activities of traditional estimation mentioned above, it is observed that the population conserves good conditions in its skin and hair, attributable to the daily use or for annual seasons of this food- These active ingredients are also present in the rest of the plant and in the seeds, as it is said in the "popular voice" " The fruit of the Calocarpum, Chrysophyllum and Lucuma has many uses in the traditional kitchen and of considerable economic price (ref 8,10) and the non-consumable parts as food have different uses in cosmetic, food and medicinal preparations for local topical use, traditional rustic (ref 4,5), which do not separate the principles and identify the particular effects in isolation or to separate the toxic compounds (ref 4,13,20), and to enable some substances of the vegetal matter . The use of home-made cosmetic and medicinal dermatological remedies of traditional use, it is not possible to be used widely for reasons of aesthetics and appearance that fail to isolate the active material linked to the plant material supporting the plant (or structure or bagasse in the seeds), which intervenes in the appearance in the applications of traditional preparations and which limit their daily and routine use, which is the most beneficial and results-oriented form of application. Likewise, the use of traditional food preparations from seeds is limited by the toxic content present, that this work presented here also resolves, making them innocuous. The traditional knowledge gives the oils of the common sapote, the Calocarpum sapota or the Lúcuma mammosum hair growth promoting activity, however studies at the University of California in Los Angeles (1970), through clinical tests carried out on the oils, fail in not finding this activity, but confirm that the oils of Calocarpum sapota are effective to stop the hair loss caused by seborrheic dermatitis (ref 4). The author of this patent application has found these same activities that are traditionally attributed to the "sapote" of promoting the growth of hair and that is poorly attributed in isolation to the fraction of oils or to the phosphatidic fraction or to another fraction of the portion of the total fixed lipids themselves, for not acting independently but in synergy with other derivatives of the seed as here disclosed (also from the seed), as described in the related application (ref 68) and in paragraphs below, however the same author of the present invention has found this same activity in other extracts from the seeds (as well as oils) that have well-defined activity on the hair follicle, increase its growth or increase the transformation of villous hair to terminal hair (ref 1,4,8) , 15,16) by stimulation with the mentioned extract. This extract comes from cyanogenic glycosidic material or related affinity (ref 6,15,18,35) that remains in the solid remnant after the oils have been obtained from the vegetable matter or all the fixed lipids or some of their fractions (see ref 68) by expression or by degreasing with non-polar solvents (extraction) or by traditional rustic techniques previously as what remains as waste. The solid residue left by the plant material of the lipid expression or the solid residue left by the lipid extraction (see ref 68) being the subject of claims in the invention of the previous related patent application [ref 68] and of the same application), which are chemically hydrolysable (ref 23,35,39) or mainly enzymatically (ref 32,40,41,42,45,51,52,53,54, 58,64,65) as described further forward and extractables as mentioned below and discussed in references 3, 6, 15, 18 and 35. The present author has found that it is a cyanogenic glycoside and the structurally related glycosides of the extract containing the lucumin cited in references 6,15,18 and 35, and their related glycosidic compounds, lucuminic acid and lucuminamide which are enzymatically hydrolyzed exogenously in the present invention to give the aforementioned aglyconic extracts, genins and toad genins with the hair follicle stimulation activity and other activities as mentioned above and detailed below. To this extract of aglyconic derivatives that we will call here sapogeninic extract because it comes from the sapogeninic glycosides of the family and the related genera which can act in synergy with the non-volatile oil portion (the total fixed lipids) and with the volatile lipids, (the genic extract, a portion of "etheric" lipids that is also used here as a matter of claims in the present application) of the same plants coming from the seeds of the "sapotes" which are the protagonists of these activities. Like the sapogenin extract or sapogenins you also get the genic extract that we will call here indistinctly as genins (or the essential oil or volatile oil fraction) both coming from the same related glycosides related to the cyanoglycoside of the present work of the "sapotes". The geninic extract is constituted as the fraction of essential oils (or some of the essences) of the "sapotes" that together with the sapogeninic extract, both properly formed together (when starting from the related glycosidic extract afin in the form whole or total or of some preparation of seeds) and is obtained as a fraction of volatile material flavored, insoluble and soluble in the water of the "sapotes" with well defined biological activity. From the preliminary tests it was found that of the cyanogenic glycosides and related structurally related glycosides, contained in the raw seeds and after being practiced non-polar expressions and / or extractions, both lipids, is where the extracts of the genins come from. and the extract of the sapogenins respectively (ref 6,15,35) as aglycones of the compounds and that by means of extraction processes (ref 3,6,15,35,56), or cleavage hydrolysis of the "molla" of the sugars (ref 3), 6,12,15,23, 35,39) and separations (ref 6,14,25,28,35) is how these hydrolytic derivatives of the support structure and amyloid germinal nutrition of the seeds are obtained. It has also been learned that the genins constitute an odoriferous principle aromatized to the plant material in an isolated form and that in the topical application it is a selective rubefaciente and contrairritante agent for some epithelia such as the face and the scalp mainly, which only produces irritation of the type of hyperemia, heat and pruritus in the applied tissue and for use in cosmetology and dermatological pharmaceutical compositions and with the possibility of food uses. The sapogenins in combination with the genins (both volatile), in conjunction with the non-volatile oil fraction (ref 68) (which have synergistic activity demonstrated in preliminary tests, that being the pharmacological investigations carried out by the present work and in the facts exposed previously and later, it is an agent with topical defoliative activity, enhanced by the other lipid fractions, volatile and non-volatile of the same seed, with positive effects in the renewal and stimulation of the development of the epidermis including these effects to the hair follicle; also with activity as a percutaneous absorption agent for a continuous aqueous phase in conjunction with a dispersed lipid phases or in hydrated lamellar lipid phases or as vesicles of the type liposomal that conforms them or contain them and their feasibility to pass to deep layers of the epidermis and with the variation of bacteriostatic and fungistatic effects, which also produces the referred extract or as purified substances. Together these substances or extracts, the genins and the sapogenins or in the form of isolated compounds plus their individual activities or in synergy with the fixed lipids, the latter being also the "sapotes", (the lipids are the subject of another patent application) previous related by the same author ref 68) and of the structurally related glycosides related to the cyanoglycoside of the Calocarpum, the Chrysophyllum or the Lucuma, the industrial manufacture of a range of cosmetics and pharmaceutical products for topical use is possible with the mentioned effects and made for this purpose as results of the present inventions; the geninic and sapogeninic extract is also feasible to be used as flavoring agents and preservative against microorganisms respectively, in foods as alternate uses or substitutes of the extracts and derivatives presented here. The objective in the matter of this invention is to make cosmetic and pharmaceutical dermatological preparations with renewal properties of the epidermis and of scalp preservation promoters and characterized by having substances of Calocarpum, Chrysophyllum or Lucuma, the "sapotes" as derivatives of cyanogenic glycosides and related related glycosides, genins and sapogenins plus total fixed lipids, fixed oils, lecithin (phospholipids) and unsaponifiable lipid material such as glycosidic residues [triterpene and steroidal or sterols] (ref. 67.69) components of the non-volatile oil fraction (ref 68) plus cyanoglycoside and related structurally related glycosides, all with biological activity. Fixed lipids (or the non-volatile oil fraction) (ref 68), with collateral or synergistic activity for the aforementioned volatile and insoluble oily material, the second formed by the aglycones, the genins and apart from the sapogenic derivatives, also volatile but soluble in water, respectively, which are obtained and their corresponding glycosides from where they come from and which are obtained in a different extractive and separation processes, all containing beneficial activities at a minimal dose in the skin such as hydration more to the activities mentioned above. The cyanoglycoside and related structurally related glycosides of the "sapotes" apart from their biological activity are used mainly as hydrolysable substrates as a whole or in isolation or by their presence in extracts and preparations or in the treated or raw seed. The dermatological cosmetic or pharmaceutical preparations according to the invention preferably contain the active compounds in pure or semi-purified form, or of plant extracts or hydrolytic products or expression products, all of which are soluble and easily treatable and can be handled and safe, without obvious incompatibilities. which contain the active ingredients. These include the mixture of glycosides structurally related to the cyanoglycoside, the genins, the sapogenins or the mixture in combination of sapogenins and genins, within which the volatile oils are constituted, which are described below and the fixed oils which are constituted as a mixture of oils bound with non-volatile fatsidic material as a fixed oil fraction, forming the total fixed lipids, plus another fraction of unsaponifiable (lipidic) material such as triterpene and steroidal glycoside residues or "sterols" (no cyanogenic), (ref 18,19,27,69) This is why they are called "total" fixed lipid fraction, because they are conformed by the sum of non-volatile lipids present in the seed. These last five are grounds for another patent application related by the same author (ref 68).

DESCRIPTION OF THE STATE OF THE TECHNIQUE. CLOSEST. It is known that "sapotes" contain lucumin and its chemical structure has been elucidated (ref 6,11,15,35), although it is not known in the literature or in practice the transformation of this same glycoside and glycosides related structurally related (to the first), endogenous enzymatic with the own enzymatic mechanisms of the seeds of natural form; or exogenous, extrageneric, using enzymes of another plant or microbial genus, to genic aglylic hydrolytic derivatives such as benzaldehyde or of the other related glycosides, lucuminic acid and lucuminamide transformed under conditions equal to the saponinic aglycone hydrolytic derivatives such as hydroxy compound with characteristics acids similar to mandelic acid and amide of the latter from this group of glycosides of the "sapotes" of these genera, and their use in cosmetics and dermatological compositions with pharmacological activities. By means of chemical synthesis it is possible to reach the benzaldehyde (ref.22) and the racemic mixture of mandelic acid (ref.28,29,30,31,39) which are the analogous compounds closest to those found in the extracts of the cyanogenic glycosidic hydrolysis products and the glycosides related to the latter of the plant material from Calocarpum, Chrysophyllum and Lucuma. The cyanogenic glycosides such as amygdalin, prunasin (which would be the closest analogs of lucumin, the cyanogenic glycoside existing in the "sapotes"), which are obtained mainly by extraction of plant matter of different genera such as Prunus. species of bitter almond, peach, peach etc. or racemic mixtures of amygdalin analogues can be chemically synthesized, such as those referred to in the controversial ref. 13, of the analysis of an indecisive and finally rejected activity on the patent of these chemical analogs of lucumin, and of the derivatives of leatrilas such as Leatrile ™ (registered trademark) ref. Brit. Pat. 788,855 (1958) and U.S. Pat. 2,985,664 (1961), which is the synthetic derivative "of the amygdalin or of the beta glucuronide derivative of mandelonitrile or of prunasin" (ref 11, 12 and 17 but referenced therein). The amygdalin and the prunasin are also obtained from a natural plant extractive source as previously mentioned. The extracts obtained with the cyanogenic glycoside and the related structurally related glycosides of the "sapotes", are mainly used in the present invention for the preparation of the hydrolytic derivatives such as the genins and sapogenins, deglucosylated, denitrilated, detoxified derivatives of the lucumin and like that of related glyconnes. In the case of "sapotes" the hydrolytic extracts obtained from the cyanogenic glycoside present in the plant material, which is the benzaldehyde derivative in the genic extract and the hydroxylated carboxylic acid derivative extract and the amidic derivative with characteristics derived from the same acid (mandelic or mandelamide respectively) in the sapogeninic extract. The sapogenins are enriched by different substances that are components of the extract obtained from the vegetable material of the "sapotes" and, apart from that, no energetic or "rebellious" chemical reagents are used in their extraction or separation and minimal extractive materials are used in the production. obtaining of these aglyconic derivatives as extracts of hydrolysis products (aglyconic), chemical or mainly enzymatic hydrolysis. These two aglyconic extracts, genic and sapogeninic, are obtained as a group of substances joined together, extractive, characterized by the same equivalent process of obtaining and by their biological activity and for its chemical properties of biological origin, such as extract of genins and extract of independent later sapogenins, with a minimum cost and labor impetus in obtaining, compared to the synthetic process that requires costly chemical reagents, expensive manufacturing equipment and expensive purification techniques and the most important that is obtained with different chemical, biological and varied pharmacological activities. The same happens with the extractive process from the plants mentioned above different from the "sapotes", as for example in the hydrolysis process of the amygdalin and of the prunasin to give benzaldehyde compounds. These processes from the plants mentioned above different from the related "sapotes", suffer from the methods of the present invention that do not define ways to derive by hydrolysis, mainly exogenous extrageneric enzymes as in the present invention to benzaldehyde and hydroxy compounds ( ref 39) as to the acid and its amide from the group of glycosides related to the lucumin of the "sapotes". In contrast, lucumin and the related glycosides related to it, of the "sapotes" by the techniques referred to in the present invention, in which methods of obtaining and using them as an enzymatic algiconic hydrolyzate using exogenous extrageneric zymogens (from other genera) to obtain the benzaldehyde derivatives as genin extract and also achieve hydroxy compounds with characteristics of benzylic cc-hydroxylated carboxylic acid, similar to mandelic acid and its amide, mandelamide or sapogenin extract and its use in cosmetics and dermatological pharmaceutical compositions, all these being the object of the present invention. With this hydrolytic process practiced to lucumin and related glycosides structurally related to this of the "sapotes" but mainly with the process of exogenous, extrageneric enzymatic hydrolysis, characteristic extracts are obtained with different substances that are obtained by the same process of obtaining and separation described below, constituting volatile organic matter of the "sapotes" from the glycosidic portion related to the cyanoglycoside (crude content in the seeds or in isolated form as whole glycosidic extract constituted by the three related glycosides). The hydrolytic products are the genins or the genic extract (or the volatile oils or essential oils or the essence of the "sapotes") and together constituted by other volatile organic substances obtained and carried in the same process of separation / insoluble and soluble to water respectively from the vegetable matter of the "sapotes". The sapogeninic extract with acidic and amidic characteristics, hydroxylated as defined below. These genomic and sapogeninic extracts are constituted as the products of exogenous, extrageneric enzymatic hydrolysis of the cyanogenic glycosides and related structurally related sapotes. The sapogenins have the possibility of being crystallized as a hydroxy compound with characteristics of carboxylic acid and the amide carbonyl derivative of this hydroxylated acid; likewise the benzaldehyde of the "sapotes" is obtained in the form of an isolated extract. It is described in the literature (ref.22) that benzaldehyde is obtained by chemical synthesis and is obtained with chlorine contamination or by oxidation products of toluene to differences of the genins of the "sapotes" which are characterized by the peculiar smell of the related plants and that is obtained without contamination of chemical reagents such as benzal chloride used in chemical synthesis or by oxidation compounds of toluene in other forms of synthesizing it. Benzaldehyde also occurs in certain seeds such as those mentioned above, different from the related "sapotes" that contain amygdalin (ref 39), such as bitter almond, also known as Prunus amygdalus. In the bitter almond are own enzymes, functional, endogenous (own internal glycosidic metabolism [glycosidases] of the seed), of this genus, which catalyze the hydrolysis of susceptible glycosides, which is found in the tissues of matter vegetable, as of the seeds of the cited almond (ref 22,42,51,53) and are trivially called "emulsins" (glucosidases); that the seeds when crushed come into contact with the glycosidic substrate also present in the same ground seed, breaking the endogenous cytoplasmic compartments of the tissue, subcellular or extracellular (ref.65) that contain them and the hydrolysis of the glycosides present in stay of water, and they react giving benzaldehyde or the essential oils of bitter almonds. These essential oils of the bitter almond are different organoleptically and with some other characteristics different from the essential oil of the related "sapotes", which also contains benzaldehyde but with a series of particular compounds that are accompanied in the extract that characterizes the peculiar essential oil belonging to the volatile oils of the vegetables related to the "sapotes" with their characteristic aroma from which they come, being able to include from the hydrocarbons (terpenes and sesquiterpenes), alcohols, acids, asters, aldehydes, ketones, phenols and lactones of the same plant with volatile odoriferous and different characteristics, which distinguish them from other genera, such as those mentioned other than "sapotes" (ref 3,17). It is known that of the genus Prunus amygdalus, the bitter almond is obtained from benzaldehyde, this is because in the seeds of this plant there are endogenous enzymes, tonsils, glycosidases (or "emulsins" as they were formerly known) particular of this genus of plants that they hydrolyze the tonsillar glycosides themselves (ref 3,17,39,40,41,42). In the genera Calocarpum, Chrysophyllum and Lucuma there are glycosides in particular to lucumin and related glycosides structurally related to cyanoglycoside, which in chemical structure very similar to the amygdalin and the glycosides related to these genera correspondingly, (amygdalic acid [or amygdalinic acid] ] and amygdalin) but which vary in their disposition and concentration and what is more in the sugary residue of these glycoside genera, the gentobiose in the amygdalin, as a sprout in the "sapotes" and their related related glycosides, but which in the aglycone residue are similar to each other; but there are equally differences in the concentration, disposition and inability in the genera Calocarpum, Chrysophyllum and in L cuma to be hydrolyzed in homogenized grated exclusively from their own seed. This is grated a homogenized substrate-zymogen own, endogenous of the same seed present and reactive individually (without the approval of the human inventive activity as presented here); with the technical manipulation of the oduction of external zymogens, exogenous that carry out the unrealized reaction, of aglycone hydrolysis in the "sapotes" (ref 40,41,42,64,65) and not being transformed by them biological catalytic systems of non-functional rnal development and not to be modified to the genins and sapogenins in the non-germinal seed state. In contrast, as described in the present invention with the oduction of exogenous, extrageneric zymogens (external of another plant or microbial genus) and therefore being used for this purpose, exposed here as an inventive step, to obtain the cyanogenic glycosides and related relatives. more to its aglyconic hydrolytic derivatives, the genins more to the sapogenins. The transformation described in the present invention to aglycone derivatives (the genins and sapogenins), non-toxic and the use of the extracts or related compounds of the "sapotes" mentioned in the following cosmetic uses and in dermatological pharmaceutical compositions is the objective, without prior or current industrial practical use to the present invention , being wasted or unnoticed its potential as a usable natural resource, in which apart from the benzaldehyde genins are also obtained the sapogenin extract containing the carbonic hydroxy compounds and being used all in the form of extracts or in purified form with the same purpose, which is to make cosmetics and dermatological pharmaceutical compositions or in alternative uses, substitutes or potentials as in food uses (as flavoring or conservative as for example), but with properties different from existing materials and products, as described above and later. The lack of the biochemical event due to the metabolic catalytic incapacity characteristic of the seeds of the related "sapotes" (ref 64.65), is the untapped benefit as a human demand to be a modern cosmetic, medicinal and nutritional resource transformed, separated and obtained by hydrolytic reactions, in soluble and insoluble (to water) and volatile materials with pharmacological properties, as are the volatile essences and the a-hirdroxy compounds, obtained by the inventive activity developed here, which are separated into fractions in the form of an oil and others soluble in the aqueous medium; the first with characteristic aroma to the vegetable, the second with dermal properties mainly among others and the fact of the technological innovation also presented here of its uses, interceding technically, in the obtaining being transformed the glycosides related to the cyanoglycoside of the "sapotes" by the exogenous, extra-generic enzymatic replacement of aggregated ß-glucosidases ex profeso of peculiar, adequate, convenient and appropriate external means of different plant or microbial genus and thus enable their transformation and use to both groups of extracts, the genins and sapogenins thus achieved; these are the reasons for the present invention and as a technical advantage over known processes such as the amygdalin, the bitter almond that only transforms into the benzaldehyde genins with their own, endogenous enzymatic systems, this is without achieving hydroxy compounds with carbonyl characteristics as to the related carboxylic acid or its amide, lucuminic acid and lucuminamide (corresponding to the related glycosides derivable in aglycones).

The extra generic enzymatic replenishment, a motive of the present invention, results in a profitable process different from the existing ones with a minimum cost in the financing of the process that is limited to the seeds of the related "sapotes" and to the replacement of a suspension zymata of suitable, convenient, appropriate "emulsins" (of a different kind), capable of making the mentioned transformations in all the glycosides related to the cyanoglycoside and described below. This method of exploitation arises as an innovative procedure by treating the homogenized seeds of the "sapotes", which are the plant material that are not used more favorably than as mentioned here and of the exogenous zymogen, of the reaction container (or reactor) more united of the implements of separation of the products. Other favorable uses other than the uses of this invention may be as foot of graft or graft, or that used in another more profitable form than could result from the facts of these descriptions and from the above related patent application by the same. author (ref 68), as in the employment in inconsistent and incomplete usuras as rustic and traditional jobs in relation to the uses that it is possible to give in context to the present work, or worse, jobs that fail to separate the pharmacologically active material from the constituents of the seed that make them inappropriate, toxic (if they are ingested) or inapparent and can be used in the processes described in this section and later, and in the related request (ref 68). The most profitable uses described here are in the use of raw and processed materials obtained by this work to make elaborated products of commercial use of employment by the society that use ingredients of the seeds of the "sapotes", as here reported, as to the aglyconic derivatives or genic and sapogenic extracts, glycosidic preparations related to cyanoglycoside and more fixed lipid extracts in total or partial composition and being exploited industrially as a renewable resource of the seeds through their transformation to consumer products such as cosmetics and dermatological pharmaceutical compositions or in alternate, substitute or potential uses (such as pharmaceutical raw materials in food uses) and other uses already described. For this and the above mentioned the use of the seed of the "sapotes" as described in the present invention results with technical advantages with respect to the already known processes in which the resource of the seeds of the "sapotes" in its totality being waste material of the fruit and very little for other purposes or its null or almost no use in processes of profit, such as transformation, procurement and use in derivatives or extracts such as genins, (the benzaldehyde derivatives), free of chemical reactants, accompanied by a series of volatile substances that characterize this volatile extractive oil or essential oil and with the coproduction of the sapogenins or the sapogenin extract composed of a hydroxylated acid and the amide of this acid which is also carried out by the process, the subject of the present invention or in contrast to the use of mandelic acid obtained by synthesis chemistry with vigorous reactions giving reaction mixtures, without obtaining the amide of the related acid, as in the present invention. Another difference is the equal form of transformation, of obtaining and using the sapogenins of the "sapotes" also achieved in a subtle way, by biochemical reactions enzymatically and by gentle separation techniques, against the other processes to obtain "analogues" unqualified. Another technical advantage of this invention is that volatile oils are obtained from the "sapotes" (essential oils) potential pharmaceutical and food ingredients that do not exist in the form of "sapote" essence and less used commercially, which is also characterized its transformation and its obtaining process, in its peculiar aroma to "sapote", different from the known, existing, natural isolated vegetal aromas.

Also the objective of the present invention is to solve the problem of the rustic use that has been used in traditional cosmetic (or beauty), medicinal and nutritional uses that distinguish the beneficial effects on the skin, the hair and in different conditions and in diverse uses. as in food or in customs based on preparations of Calocarpum, Chrysophyllum or lucuma but without being identified their legitimate individual action, isolated principles, separated in a productive way these pharmacological, cosmetic, medicinal and nutritional principles, detoxified in their use, the vegetal matter. The cyanogenic glycosidic principles and glycosides related structurally related to this, of genins, of sapogenins and as an alternate agent of these effects (6 synergists) to fixed lipid extracts in total composition or in fractional or partial form, all from Calocarpum , Chrysophyllum or Lucuma and incorporate them into effective and current cosmetic preparations or dermatological pharmaceutical preparations as a solution to the technical problem in the use of very dissimilar analogs or in traditional use (rustic uses) as technical novelties in modern cosmetic and pharmaceutical compositions dermatological treatments that are effective for the defoliation (desquamation) of the superficial corneal layer and the epidermal renewal, including these effects to the keratinocytes at the level of the hair follicle, which is stimulated in its growth and development, as well as in the prevention of the anticipated deterioration of the skin and scalp and as agents that favor percutaneous penetration in conjunction with a lipid phase, which may be forming a hydrated laminar lipid phase or in liposomal type vesicles; likewise in uses as bacteriostatic and fungistatic agent "win vitro" and "in vivo" are the subject of the present invention, enabling alternate, substitute or potential uses as in food uses and uses as pharmaceutical raw materials as for example as prophylactic, flavoring, acidifier, antioxidant, buffer (pH), emulsifier, flavoring agent, vehicle, etc. in connection with the preceding related patent application (ref 68) The sugary residues of lucumin and the related glycosides related thereto are also used as Alternate surplus resources, in glucose and xylose or as a disaccharide primaverose or in the form of an extract or in the residual "zymogenic" wort as a resource of transformable metabolites in biomass, in the integral use of this plant medium (ref. 14,15,26, 35.53 and references cited therein: ref 56.61).

DESCRIPTION OF THE INVENTION. The present invention relates mainly to the cyanogenic glycoside, lucumin and its related structurally related glycosides, lucuminic acid and lucuminamide or to the corresponding aglyconic hydrolysis derivatives of these which are the sapogenins and genins of Calocarpum, Chrysophyllum or Lucuma. . Present in the leaves, in the flowers, in the bark, in the sap and in the seed of the fruits is the toxic cyanogenic glycosidic compound, lucumin and also for the glycosides related structurally related to the former, lucuminic acid and lucuminamide. (ref 4,15,18,20,35,56), secondary metabolites of this family (Sapotaceae) and related plant genera (Calocarpum, Chrysophyllum and Lucuma). In a particular variant of the invention the cyanogenic glycoside and related structurally related glycosides, genins and sapogenins respectively as derivatives of the former (but each of these groups of extracts with independent and individual processes of obtaining), are obtained by extraction, or the aglyconic derivatives are obtained by chemical or enzymatic hydrolysis and then by extraction, being able to be of the bark, leaves, buds, flowers, of the sap, of the peels of the fruits and of the seed of the fruit of the Calocarpum, Chrysophyllum and of the Lucuma. Preferably in particular the plant tissue is selected from the seeds, as an easily renewable resource which are dried and degreased by lipid expression techniques and followed by lipidic extraction with mainly apolar solvents, (techniques that are the subject of another patent application) by the same author [ref 68]), which are practiced for the purpose of mainly recovering and preferably avoiding these fixed lipids in order to obtain glycosides related to cyanoglycoside if these are the extracts and compounds of the subscribed ones, those that you want to get to obtain. To obtain the related aglyconic hydrolysis (glycoside) derivatives do not interfere with the fixed lipids, in the process of obtaining them, but the preliminary recovery in anhydrous form of the fixed lipids is more profitable before proceeding to the aglyconic hydrolytic operation involving the use of water Plant matter such as the seeds of Calocarpum, Chrysophyllum and Lucuma in degreased and powdered form, by breathing these powders through the nose irritate the respiratory tract and cause sneezing as reported by the activities of sapogeninic glycosides (ref 3). These and the related aglycones can be prepared from the seeds, arranged in various ways to obtain them, the main ones being those described above and in the related patent application (ref 68), on obtaining the solid residue of lipid expression, this is in the form of "chicharrón" solid residue of the lipidic squeeze and or as "degreased flour", solid residue of the lipid extraction, with solvents, as vegetable remnants in the production of oils or fixed lipids of the "sapotes". The productive function of recovery of total fixed lipids is performed, which is a preliminary trade, merely as an execution that pays dividends and should be performed before the operations to obtain the genins and preferential evasion to obtain the cyanogenic glycosides and structurally related to the cyanoglycoside. The sapogenic glycosides (which produce foam in aqueous medium, such as the cyanogenic and related sapotes) and their aglyconic derivatives are also obtainable from the seeds as raw materials, plainly ground or ground.

To obtain the cyanogenic glycosides and related affinities it is possible to use the plant material directly as raw seed, plainly ground or crushed or preferably it being possible to use the vegetable matter degreased totally or partially, as the residual solid that remains as a remnant after the lipid expression of the seeds (partially degreased) and or as a solid remnant of the oil extraction (to see the obtaining of the total fixed lipids of lipid expression or extraction of oils and of its residual derivatives see the previous related patent application of Calocarpum, Chrysophyllum or Lucuma (ref 68) With the operation of degreasing the seeds in particular with the process of lipid expression, a lipid portion consisting of the fixed lipids in total constitution (fixed oils, phospholipids and lipid glycosidic residues labile to the lipid separation (not cyanogenic) triterpenic and steroidal or here called the sterols, mainly; removable by the techniques in use in the present invention and in the preceding related application (ref 68) for the degreasing of vegetable matter (expression and extraction) as a fraction of oily substances obtainable in preliminary procedures to another fraction of lipids derivable from the glycoside cyanogenic which correspond to the volatile oils described here as the genic extract or the genins; these with alternate, collateral, secondary or synergetic activity for the main purposes of this description and which are the reason for this request and the related patent application by the same author (ref.68). As mentioned in the previous paragraph it is possible to use the whole seeds, roughly ground or crushed for the preparation of the aglyconic derivatives of the glycosides related to the cyanoglycoside or to the latter, without any of these procedures or raw treatments ground or crushed, degreasing, extractive or hydrolytic are dependent or restrictive of one another in the starting raw material; it is possible to start obtaining the aglyconic derivatives of the raw seeds (plainly ground or crushed) or the seeds degreased or of another preparation of the seeds or that of the glycosidic extract related to the cyanoglycoside or of this extract in some other later stage in its purification. As outlined in the previous paragraph it is possible to arrive at the glycosidic extract related to the cyanoglycoside or to this extract at some later stage in its purification starting from the raw seeds (plainly ground or crushed) or the seeds degreased or of another preparation different from the seeds. The vegetable matter of the seeds of the "sapotes" are constituted by lipid material different from the fixed lipids, this different fraction of lipids contain volatile oils that are derived from the cyanoglycoside that are intimately bound to the structural matter by means of sugars that they are joined in long chain carbohydrates in the sustenance and germinal tissues (in the cotyledon) combined in the glycoside in the seed. Some of these glycosidic substances consist of a disaccharide linked to hydroxy compounds of nitrile, carboxylic acid or amide derivative of these related glycosidic substances which are hydrolysable from the carbohydrate portion to aglycones in special treatments and can be extractable and subsequently separable. In the present invention this aglyconical hydrolysis is carried out directly from the seeds independently of also having the possibility of being extracted, from the same sources in another process, to the glycosidic material related to the volatile oil and to this glycosidic extract to practice the hydrolysis to the aglycones. related above; these volatile lipids are recognized here as the genins and the others as the aforementioned sapogenins of the "sapotes". The aglyconical hydrolysis in the present work is carried out mainly with the introduction of exogenous, external, extrageneric enzymatic products of other vegetal (or microbial) genera, however, this hydrolysis is not carried out in a natural, common way, in the Seed in isolation, with the same enzymatic mechanisms of the same seed of the "sapotes", prepared as a single material independently, simply ground (in aqueous medium); it requires the introduction of a second alternative, external, exogenous, adequate, peculiar enzymatic system to achieve the conversion to the derivatives of the genins and sapogenins; and subsequently separation techniques of the hydrolyzate extracts are used. These aglyconic derivatives retain the alpha hydroxylated (alcoholic), nitrile, acid and amide characteristics respectively of the glycosides from which they are derived (ref 11 and 15). The first of these derived substances, from the previous mention, is converted to benzaldehydica, oily and volatile genins, an oil different from the fixed lipids mentioned above, insoluble to water, obtainable by the same enzymatic reaction or subsequently from mandelonitrile spontaneously (ref. 65 and references cited therein) as a hydrolyzed nitrilic glycoside (cyanoglycoside); the last two substances of the hydrolyzate being the acid and amide extract derived from the previous mention, as well as separable and recoverable, dissolved, soluble in the aqueous hydrolysis medium. In this way, the extract of genins and sapogenins are obtained as a volatile extract, respectively, the former with characteristics of essential oil, the latter with other characteristics also of great pharmacological importance, as stated above and below. The genins are obtained as aglycones of lucumin, the cyanogenic glycoside and the sapogenins of the glycosides related to cyanoglycoside, lucuminic acid and lucuminamide of Calocarpum or of Chrysophyllum or Lucuma, (or the "sapotes" referred to). In another creation the cyanogenic glycoside and related glycosides structurally related to the first, the derivatives of aglyconic hydrolysis of these genins and sapogenins are derived preferably from the sapogenic glycosides (foam producers) such as lucumin, lucuminic acid, lucuminamide and are obtained benzaldehidicas geninas and acid and amidic sapogenins related to the previous glycosides. These obtainments of the aglycones are possible their achievements without being necessary to extract them, to obtain them separately or to separate them to the glycosides in advance, being able to be obtained from the raw seeds, ground or from some other products of them, as partially or totally degreased, as mentioned above, without being restrictive any treatment in obtaining the derivatives Mention may be made of seeds or cotyledon that are plainly ground or crushed or, if desired, of the related glycoside extract isolated or some further step in its purification. Sapogenins are made up of volatile material, like genins or volatile oils; the latter are used in the present invention as a rubefacient and selective irritant of delicate epithelia (face and scalp of the head) which opens the pores of the skin in cosmetic preparations or dermatological pharmaceutical preparations.

On the other hand, the activities that unfold the sapogenins that result as defoliative agents that promote the renewal of the skin and the stimulation of the development of the epidermis including hair growth and retardation in hair loss, to combat the effects of aging epidermal and the anticipated deterioration of the skin in topical preparations, also with action of percutaneous penetration enhancer. The enhancing property of the percutaneous penetration of Sapogenins, in preparations based on these, are shown in aqueous solution in conjunction with a dispersed lipid phase or with a hydrated laminar lipid phase or liposomal-type vesicles containing them (ref. 2,33,34) and positive effects for repeated use in treatments for the improvement of damaged skin and hair, and also with the variation of bacteriostatic and fungistatic effects that also exhibits "in vitro" and on the skin. The glycosides referred to in the preceding paragraphs are characterized by being β-D-diglycosides (ref 60.66) and consist of lucumin ([6-0- (β-0-xylopyranosyl) -β-D-glucopyranosyloxy] -2 phenylacetonitrile) and two related related glycosides derived from the former, lucuminic acid ([6-0- (β-D-xylopyranosyl) -β-D-glucopyranosyloxy] -2-phenyl carboxylic acid) and lucuminamide (6-) 0- (ß-D-Xylopyranosyl) -β-D-glucopyranosyloxy] -2-phenylacetamide) or related cyanoglycoside derivatives related to CalocarpiMi, Chrysophyllum or Lucuma (ref 15,35,56), which are mainly constituted by aglycones of the alpha-hydroxynitrile type, the alpha-hydroxy acid derivative and the alpha-hydroxylated amidic derivative of the latter acid, and a carbohydrate (disaccharide), the primaverose as a sugar residue of lucumin and the two related related glycosides , in which different methods are used in their determinations, including s the indirect photometric and direct chromatographic method, which may be qualitative, semiquantitative or quantitative, as described in references 11,14,15,23,24,25,35,36,37,38,43,46,55,56 , 57,59, 62,63 or enzymatic references 32,40,41,42,44,45,46,48,49,50,51,52,53,54,58. The cyanoglycoside, the lucumin of the "sapotes" and those that are included as the structurally related glycosides related to it, recognized as lucuminic acid and lucuminamide (ref 15.56); found in the present work with activity against ß-glucosidases (tonsillases) and other denitrilases (hydroxynitrile liases or mandelonitril liases) in conjunction with other enzymes constituting complex exogenous catabolic systems, which are absent or inactive in the stage of seeds, non-germinal In the related "sapotes" (ref 51.65 and references cited there), it has been found in this work that lucumin by this exogenous catabolic activity gives mandelonitrile and subsequently benzaldehyde, lucuminic acid and lucuminamide give a compound similar to mandelic acid plus another amide derivative of this same acid, both the acid and the amide found here that contain characteristics of hydroxy compounds in alpha position, adjacent to the carbonyl of the functional groups in mention of the compounds respective, as well as where they come from, of the related structurally related glycosides, but cleaved from the sugary residues (ref 11,15, 31,35,36,37,38,39,46,51). The glycosides related to the non-practiced aglyconic hydrolysis can be obtained and separated in an extractive process for glycosides (ref 3) independently of the present process of enzymatic aglycone hydrolytic derivation. The enzymatic algicónico hydrolitic process can start, without being dependent or restrictive, with the related glycosidic extract or in some other more advanced stage of its purification, being more elaborated the obtaining of these aglyconic hydrolytic derivatives and more expensive to obtain them starting from these stage of elaboration of the related glycosidic sources. It is more convenient to reach the aglycone derivatives hydrolytic enzymatically start the derivation with some product of the seeds plain ground and raw or defatted partially or totally, as of the residual solid products of expression and extraction, both lipidics respectively. Be part of plant material that has no value or minino commercial value such as leaves, husks or seeds of the fruit removed from the pericarp. Preferably the latter are used, and endocarp (or seeds). The plant material is selected from which there are changes in coloration due to oxidation or degradation with saprophytic flora caused by excess moisture. From this selection they are dried from the natural moisture of the fresh seed (without excessive drying), cut into pieces or crushed, degreased as indicated above (or by reference 68) and is extracted by means of chemically pure solvents selected from water, alcohols preferably containing from 1 to 4 carbon atoms and from esters containing from 3 to 6 carbon atoms or by means of solvent mixtures based on any combination of the solvents above, if you want to reach the glycosides related to cyanoglycoside. When cyanogenic glycosidic extraction and structurally related affinity at the end of the extract is filtered and concentrated by evaporation to dryness under reduced pressure to give a primary extract of concentrated glycosides according to the present invention. In a particular variant, the use according to the invention relates to mixtures of glycosides related to lucumin which are obtained according to the present invention as a concentrated extract or as a dry extract by the procedures indicated in examples 1,2 and 3 below. The aforementioned primary glycoside extract is treated with the introduction of an apolar solvent or solvent of intermediate polarity and after being stirred in it or the solvents which are preferably miscible with the primary extraction solvent such as ethers or low molecular weight ketones. in particular ethyl ether or isopropyl ether, acetone, ethyl methyl ketone among the main ones. The amount by weight of the apolar solvent or intermediate polarity used is from 1 part to 100 parts of the solvent on the one hand of the material of the primary extract of the aforementioned glycosides. The insoluble material and or the formed precipitate contains a mixture of the related structurally related related glycosides in semipurified form according to the invention. It is possible to further purify some of the glycosides related to the cyanoglycoside by recrystallization by some accessible method with polar solvents or water, or used as a total extract, according to the process in the invention. Still further it is possible to purify the above glycoside mixture by means of the redissolution of the insoluble material or the precipitate in volumes ranging from 1 to 20 parts by weight in water. The aqueous solution is extracted 2 to 5 times with an alcohol having poor solubility in water such as n-butyl or iso-butyl alcohols, which has been saturated with water, in proportions which may be for example 1: 1 in volume for each extraction operation. The alcohol extracts are separated from the aqueous phase, combined and evaporated under reduced pressure. The residue of the evaporation is redissolved in 10 parts of its weight in water and dialyzed against pure water. The dialysate of glycoside extract related to the cyanoglycoside is freeze-dried and if necessary further purified, the lyophilisate is dissolved in the minimum volume of methanol which is evaporated and restored with absolute ethyl ether and the precipitate formed is collected. The solid mixture is redissolved in the minimum amount of water and chromatography techniques are applied to isolate it as pure compounds, techniques such as those proposed in example 3. The genins and sapogenins can be extracted from the plant material of the treated seeds directly or of the material extracted from the same treatment described in the previous and previous paragraphs and later, practiced to the extract concentrated in glycosides or concentrate rich in related glycosides, independently of the most affordable treatments starting from the preparations of the seeds, roughly plain cut, ground or crushed and partially or totally degreased; This procedure is mainly carried out by means of what are left of the vegetable solid residue by the products of lipidic expression or, more preferably, by the fact that the lipid extraction of the seeds is left from the solid remnant of vegetable matter. The genins and sapogenins according to the present invention are obtained as aglycone hydrolysates with the methods proposed here from preparations of the seed or semipurified extracts of related glycosides related to lucumin., this last procedure of obtaining from and after the glycosidic extraction related to the cyanoglycoside, is independent of the algiconic hydrolytic process that is through the direct treatment of preparations of the vegetable matter of the seeds (cut, ground, or crushed) or by means of treating the solid residue of lipid expression thereof mentioned above and the residual solid product of the lipid extract, the fat-free flour (or seeds partially or totally degreased respectively) (ref 68); being this last resource the most advantageous for the productive recovery of all the lipids preceding them, the integral use of the seed. The genins and sapogenins of the semipurified extracts related to the cyanoglycoside or preferably of some of the following preparations of the seeds are excised; as of unprocessed vegetable matter crushed or of the solid remnant of the vegetable matter after the expression (or of the "chicharrón" of expression) or of the solid remnant of the extraction (or the degreased flour of extraction) both lipidicas, of the seeds; by breaking the glycosidic bond attached to the disaccharide, by acid hydrolysis as is conventionally done (ref 15,23,35 and 39), which is carried out by concentrated or diluted sulfuric and halogenated acids. The main halogenated acids used herein are hydrochloric acid, perchloric acid, trichloroacetic acid, and are used in this work in a manner similar to those described in the above references. In other forms of hydrolyzing enzymatic means, or protein extracts or purified or unpurified preparations, isolated or unisolated from the same zymogenic catabolites (ref 47) are used without this being a limiting in the form of hydrolysis or in the origin or enzymatic form, which may be of microbial (ref 32,44,45,49,50) or vegetal origin, coming from tissues of different species and types of seeds or from different tissues of the same exogenous plant, all peculiar, adequate, convenient and appropriate as of leaves, stems, bark, pulps of fruits among others and seeds and seeds so mentioned. However specific and non-specific enzymatic systems are required, suitable, appropriate and peculiar as said above, different from those existing endogenously, or inactive, or non-functional, or non-existent of the "sapotes" related for the purpose of this work, that they are found in exogenous plant tissues and that they catalyze the transformation to free sugars and the particular aglycones of the "sapotes", this series of reactions being the most profitable form for the objectives of the present invention (ref 11,12,39, 40,41,42,48,51,52,53,54) followed by separation forms, isolations and if desired further purifications. In a type of hydrolysis that uses plant enzymes and is carried out in buffered aqueous solution, preferably with acidic medium, in the plant material of Calocarpum, Chrysophyllum or Lucuma that contains these types of zymogenic ferments or protein extracts of enzymatic systems or purified particular enzymes or purified, unpurified, isolated or unisolated, peculiar, appropriate, convenient and appropriate exogenous beta-glucosidases which, like the glucosidases of other plant genera, catalyze the hydrolysis of their own glycosides of the same genus. but it is also possible to use them exogenously in another generic type of vegetable glycosidic substrate that lacks the required enzymatic potential (endogenous functional glycosidases) (ref 3, 12, 40, 41, 53) as the glycosidic substrates related to the cyanoglycoside of the genera of the "sapotes" referred to. These exogenous systems of "emulsins" hydrolyze beta-glycosidic bonds in monomeric or dimeric units of sugars (ref 56 and references cited therein, ref 65 and also references cited therein) and in hydroxy compounds, the aglycones, which in the case of Lucumin glycosides, lucuminic acid or lucuminamide are hydroxy compounds that are benzylic (phenyleic) with nitrile or later aldehydic, acidic and amide characteristics, respectively, of applications and functionalities in various specialties. These transformations depend on the zymatic kinetic properties of the systems, the activity, the reaction rate, the optimum pH, the temperature (ref 48.51 and references cited therein, ref 52.53.54, 56,65) to "empty" into the chemical products mentioned. As in amygdalin or prunasin from the family Rosaceae of the genus Prunus as P. amygdalus and P. serótina, as obtained by other techniques to benzaldehyde, using endogenous enzymatic mechanisms, typical of the seeds of these plant groups on exposure reaching this substance as aglycone with mandelonitrile characteristics and later transformed into benzaldehyde (ref 17.39,40,41,42,51,53); situation that also happens in the lucumina of the "sapotes" except that it is necessary the introduction of exogenous (external) enzymes, extrageneric, of another gender to obtain significantly useful results in cosmetic, pharmaceutical applications as well as in food and other substitute uses, a condition that is not achieved with glycosidases ("emulsins") that are endogenous to the genera of the related "sapotes" (see example 8). By hydrolysis of the acid and amidic glycosides related to the lucumin of the "sapotes" or more specifically to the lucuminic acid and the lucuminamide respectively using exogenous, extrageneric (peculiar, adequate, convenient, appropriate) plant enzymes, the algiconic derivatives are obtained, alpha hydroxylated, acid and amidic respectively, promoted by exogenous, extra-generic, successful hydrolases of another genus (suitable and peculiar), which cleave the disaccharides as glycosides related to glycosides related to cyanoglycoside, releasing the aforementioned aglyconic products, acid and amid correspondingly. These aglycones are separated as a mixture of sapogenins (stirred with the genins). From the lucumin, the benzaldehyde genins are released by the sapogenins of the remaining glycosides related; Both groups of substances, genins plus sapogenins are obtained as a volatile extract in a union that can be separated into its components by their different properties. The solutions obtained by enzymatic aglyconic hydrolysis of the related glycosidic substrates related to the cyanoglycoside of the "sapotes", using extra-generic exogenous zymogens and using appropriate separation techniques to separate these substances, result in the solution that also forms very apparent foam, formed by the soluble sapogenins and by water insoluble material, oily and with characteristic aroma to the same plant conformed by the genins as they are obtained in a particular variant of the invention, which is to obtain aglycone hydrolytic products of the cyanogenic glycosides and related products of Calocarpu, Chrysophyllum and Lucuma by preparations of ß-glucosidases, external, exogenous, extragenéricos, appropriate (["emulsinas" of another gender], microbial or vegetal) added ex profeso, to buffered conditions of acidity. This is with the introduction of a second, adequate, appropriate, appropriate enzymatic system of different plant (or microbial) species, non-specific or specific enzyme systems in the existing glycosidic substrates of the related "sapotes", such as the alternative systems that are demonstrated in examples 4,5,6,7 and 8. The genins and sapogenins of Calocarpum thus obtained have haemolytic activity at high dilutions, mainly those which are soluble in water. By hydrolysis of the glycosidic bonds as do the exogenous ß-glucosidases ("emulsins"), mentioned here and some others not described here, to these cyanogenic glycosides and related related but carried out acidic conditions (ref. cited), it is possible to convert them to specific aglyconic products, hydroxy compounds, benzylics and carbonyl compounds, one with acid characteristics and another with amide characteristics, related with characteristics similar to those mentioned for mandelonitrile and benzaldehyde, mandelic acid and mandelamide. These aglycones, genins and sapogenins of which the most abundant are volatile substances that appear to be substances such as benzaldehyde, mandelamide and mandelamide respectively of Calocarpum, Chrysophyllum or Lucuma by the non-specific or specific exogenous enzymatic action and alternatively by the chemical hydrolysis, separated by techniques for the isolation of this type of compounds. A particular variant of the invention is that which employs non-native (non-endogenous) β-glucosidase enzymatic systems of the Calocarpum, Chrysophyllum or Lucuma, from another peculiar, appropriate, convenient and appropriate extrageneric vegetable species, mainly "emulsins". not acidic and acidic in buffered acid medium, as demonstrated in examples 4,5,6 and 7 whereby insoluble and water-soluble aglycones, genins and sapogenins respectively, mentioned above, are obtained. The buffered acid medium is achieved using acid citrate, acetate or phosphate acid buffer and mixtures of these among others or that provided by different preparations of purified or unpurified, isolated or unisolated plant tissues that mainly contain this pH and "emulsifiers" of which is preferred to acidic "emulsions" (from vegetables).

Finally the reaction that takes place on the cyanogenic glycoside (alpha-hydroxynitrile) is deglycosylation in the separation of the sugary residue, regulated by beta-glycosidases, which include enzymes that catalyze the same reaction and subsequent reactions chained in the integral reaction from beginning to end, such as hydrolases, such as alpha-hydroxylases, prunasin hydrolases, followed by mandelonitril liases; All of them lead to obtain in a similar way to this product, the madelonitrile that later becomes benzaldehyde (denitrilado) and the other two related aglycones, the acid and the amidica of the "sapotes", (deglycosylated) made by enzymes that comprise glycosidases mainly from another exogenous species, peculiar, adequate, convenient; with the hydrolases, hydroxylases and hydroxynitriliase, acids belonging to these systems used and described herein, capable of carrying out the reactions described on the related species of the "sapotes" with this afunctional, diminished or inactive activity as described in examples 4 , 5,6,7 and 8 (ref 11,12,40,41,42,45,46,50,51,52). After the enzymatic reaction has been carried out at room temperature or between 15-60 ° C and with stirring for a few minutes, then some suitable separation technique of the genins and sapogenins is used, which can be by filtration, extraction, or by some technique that uses the ability to separate this emulsified mixture of components by means of accelerated heat gradients, applied to the reaction mixture as mentioned above. Isolation of the enzymatic hydrolytic reaction mixture of the products is done when they are subjected to accelerated heat in a recovery and isolation device; the separation is carried out between 80 to 110 ° C; leaving the products of degradation of the primaverose, which are to glucose and xylose or to the entire cousin, depending on the type or strength of the hydrolysis (ref 11,15,23,26,35,39,). The genins and sapogenins of the previous form obtained are obtained in aqueous medium; The genins are obtained as liquid mixture insoluble in the medium and are separated using this property. In the aqueous phase sapogenins are found which are soluble and are separated from the aqueous medium by extraction with apolar solvents or of intermediate polarity, immiscible with water, being able to make several extractions, from 1 to 5 approximately to complete the extraction of the aqueous solution isolated from reaction. Solvents such as those mentioned in the preceding paragraph are used for said execution using, using an equal volume, such as, for example, absolute ether for the extraction of the sapogenin solution, later when they have been separated and isolated beforehand. aqueous hydrolysis medium and total extract with the genins respectively. The amount of the apolar solvent or intermediate polarity used may be fractions or from 1 part to 5 parts or more of the solvent per 1 part of the volume to be extracted. The organic phase thus extracted contains the mixture of sapogenins which are concentrated to dryness under vacuum, to be obtained as a concentrated dry extract of sapogenins according to the invention. The organic extracts of sapogenins are concentrated by evaporation and formed into the solidó concentrate of sapogenins. It is possible to use some suitable crystallization technique in their isolations. If required, the sapogenins can be purified by some suitable crystallization technique with alcohols of 1 to 4 carbon atoms and mixtures with water. This way of treating the genins and sapogenins is only as a form of indication, without implying that they are a limitation in which raw material is used as a source as explained above, being able to start from several forms of preparations of the seeds of the related species of the Sapotaceous family and likewise being able to make several types of the form of hydrolyzing as mentioned above or as in various forms of isolating or in the varied form of extraction and in those of separation of the isolated products and of the forms described here as means of exemplification and other capabilities to obtain the active substances mentioned above in the present invention. In a variant of convenience, the aforementioned sapogenins are selected from those containing non-ionized acidic carboxylic groups and their corresponding metal salts, especially calcium and sodium, to carry out the present invention (ref.31). In yet another variant of the present invention the plant material of the Calocarpum, Chrysophyllum and Lucuma mentioned above are selected from generic groups consisting of Calocarpum sapota, C. mammosum, C. huastecanum, C. viride, C. odoratum, L. cuma domingensis, L. stahliana, L. dussiana, L. cuprea, L. campechiana, L. obovata, L. hypoglauca, Chrysophyllum mexicanum, Ch. Cainito, here related as "sapotes". In yet another creation of the invention, the glycosidic extracts related to the cyanoglycoside and its aforementioned aglyconic derivatives are obtained by methods that are described later as a form of indication but do not imply any limitation in the scope of the present and in the ways of obtaining it. the active ingredients of Calocarpum, Chrysophyllu or Lucuma and its use in cosmetic preparations, dermatological pharmaceuticals and the possibility of being used in food uses or substitutes. According to the facts set forth in the present invention that is related to the use of at least one derivative of Calocarpum, Chrysophyllum or of Lucuma among the fixed lipids or fraction of them (the obtaining of the fixed lipids are grounds for another related request patent by the same author [ref 68]), volatile oils or fraction of them, and the rest of volatile substances between the genins and the sapogeninae or fraction of any of them and the corresponding related glycosides related to the lucumin and structurally predecessors of the aglyconic derivatives, from which the two previous last two groups of substances or fraction of any of them are derived in any form that could occur: as residual vegetable products of lipid material expression; in the form of a solid residue of lipid extracts of the plant material; or in another form of raw plant raw material; in the form of hydrolytic products using acids or bases; hydrolyzing using exogenous enzymes of natural plants of various tissues of the same plant or of several or of microbial origin or by the use of enzymes or zymogenic protein mixtures or of various preparations of the above; consisting of plant material from seeds or different plant tissues with zymogenic character or like extracts of plant or microbial material, such as a living zymogen or in the form of active microbial extract, or some preparation or several of them, incorporated in form of raw, semipurified or purified substances from the same plant material of Calocarpum, Chrysophyllum or Lucuma and of another plant or microbial species in which these compounds can be found that catalyze the aglyonic hydrolysis transformations mentioned, to be used in preparations cosmetics or dermatological pharmaceutical preparations, (or substitute uses) solved particularly as weak defoliative agents of the superficial corneal layer and of gradual renewal and stimulation of the development of the epidermis, to counteract the effects of epidermal aging and the prevention of the anticipated deterioration of the foot l and of the scalp including these effects to keratinocytes at the level of the hair follicle, such as the stimulation of hair growth and; The effects are also concomitant as percutaneous agents and local bacteriostatic and fungistatic agent in the skin or in cosmetic or pharmaceutical dermatological preparations, or in uses as pharmaceutical additives or substitute food supplements as said above and as demonstrated in Examples 9, 10, 11 and 12; and example 5 of the related patent application by the same author (ref 68) without these demonstrations implying any limitation of the present. According to other facts the invention further provides methods of manufacturing cosmetics or dermatological pharmaceutical products developed in particular as weak defoliative agents that promote the gradual renewal of the skin and stimulation of the development of the epidermis and to combat the effects of epidermal aging and the anticipated deterioration of the skin including hair growth, with a retardation in hair loss, in topical application preparations with percutaneous penetration enhancing activity, and bacteriostatic, fungistatic effects on the skin and in the preparations of use topic, methods comprising the use of at least some glycoside structurally related to the cyanoglycoside in question or its genins or sapogenins or their corresponding isolated components or aglyconic derivatives of the above glycosides, of the whole or fractional portion of fixed lipids t otales (ref 68) or plant extracts of the latter or corresponding products of expression or products of chemical hydrolysis of the former in pure or impure form or fraction of any of them or as they may be processed or in products may contain these in a hydrated lamellar lipid phase in which preparations with suitable cosmetic or pharmaceutical excipient carriers or carriers or dermatological or other types and for other uses are presented. In a variant, these methods first comprise that at least partially at least one of the aforementioned structurally related glycosides related to the cyanoglycoside, genins and at least some sapogenins of the Calocarpum, Chrysophyllum or Lucuma or their corresponding plant extracts or hydroxylated and related carbonyl products, aglycone hydrolysates of the corresponding glycosides or as residual solid products of expression, or as solid residues of the lipid extraction or as aglyconic hydrolysates of the raw seed material crushed or ground (ref. in which they are present in some form in dermatological cosmetic or pharmaceutical preparations in hydrated lamellar lipid phases or in liposomal type vesicles (ref 2,33,34) alone or with some other active ingredient of the "sapotes" or pharmaceutical before mentioned oc on established pharmaceutical ingredients, see reference 68 and then prepared with suitable excipients or carriers, the same lipids of Calocarpum, Chrysophyllum or Lucuma being used to form the hydrated lamellar lipid phases. The fixed lipids and their solid waste products of these plant genera are subject to another patent related request by the same author, as mentioned above (ref 68). The compositions used in the methods of the present invention may also contain a variety of optional ingredients by forming suitable excipient or carrier vehicles that are added to the active ingredients of Calocarpum, Chrysophyllum and Lucuma discussed in detail herein and in the related application. patent on the fixed lipids of these genera (ref 68), of the respective Sapotaceae family.

The compositions which are used in the methods of the present invention may preferably also contain a cosmetic, or dermatological, acceptable solid, semi-solid or liquid dermatological pharmaceutical carrier which acts as a diluent, dispersant or carrier of the active ingredient (s) in the related compositions discussed or not discussed herein. . The term vehicle excipient or suitable cosmetic or pharmaceutical carrier or as a constituent of a substitute articleas used herein means that the active substance or substances or the inert ingredients that the term describes are suitable and suitable for use in humans without having toxicity, incompatibilities, instability, diffuse irritation, allergic responses, or similar or different adverse responses. . The suitable cosmetic or pharmaceutical dermatological vehicle can comprise from 0.1% to about 99.9999%, preferably from 25% to about 99.99%, more preferably from 50% to about 99.99% and most preferably from about 75% to about 99.99% and still much but much more preferably from 85% to about 99.9% by weight of the composition. Acceptable vehicles include, for example, water, emollients and / or lipophilic or hydrophilic wetting agents, surfactants (emulsifiers), thickening agents, powders, polymers, resins, plasticizers, fillers, lubricants, binders, disintegrants, solvents, cosolvents, buffer systems, agents preservatives or preservatives, sweeteners, dyes and pigments of all dermatological pharmaceutical grade or in its cosmetic case.

The water can be used in the compositions described herein and in other preparations not presented here as a vehicle. When the water is used as a vehicle, the composition may be in the form of a solution or dispersion or in the form of an emulsion, suspension or cream; or as mentioned above, forming parts of the hydrated lamellar lipid phase, which may contain the active ingredients of Calocarpum, Chrysophyllum or Lucuma in hydrated lamellar lipid phases composed in the lipid phase with genins and in the hydrated phases of sapogenins; the hydrated lamellar lipid phase can also be formed by the glycosides related to the cyanoglycoside; the lipid phase of the liposomes or the hydrated lamellar lipid phase can also be formed by the fixed lipids in whole or fractionated form or by volatile lipids or fraction thereof of the Calocarpum, Chrysophyllum or Lucuma, these mentioned fixed lipids being the subject of another related patent application by the same author (ref 68). The emollients and or lipophilic or hydrophilic humectants may include esters, fatty acids, alcohols, polyols, hydrocarbons, silicones, waxes, triglyceride, polymer blends (gels), among the main ones. Other objects, features and benefits of the invention are clearly apparent from the following explanatory description relating to several examples which are given only as illustrative means and consequently in no way limit in any way the broader scope of the invention. In the examples that are not indicated units is expressed in percentage by weight unless some other unit is indicated. In the case of extracts, the percentage (or weight) is expressed in dry weight of the extract. Examples 7, 8 and 15 involve preparations containing fixed, residual and current lipid products of the "sapotes" (ref 68) which are included by their activity, glycosidic content and or derivatives of genins and sapogenins , performed as residual products of expression or extraction, both lipidics and as current whole lipid components; also include fractions or portions of the total lipids of current use (see ref 68) for the synergistic effect they give to the sapogenins and genins, all of the Calocarpum, Chrysophyllum or Lucuma in the renewal and development of the skin and in the stimulation of hair growth, apart from the other activities mentioned that are also reasons for This and the other preceding invention by the same author, so it is required to consult the related patent application (ref 68). Example 1 Preparation of concentrated extract of glycosides of Calocarpum, (Chrysophyllum or Lucuma). 150 g of dry seeds of Calocarpum sapota, (or Chrysophyllum or Lucuma, of the related species) that have been pulverized and degreased thoroughly with an apolar solvent, are macerated in 2 1. of ethanol, the mixture is refluxed for 2 hrs .; the solution is filtered and recovered. The residue is extracted twice more with 1 liter of ethanol, filtered and the three filtrates are combined and concentrated in a rotary evaporator at a volume of 100 ml. The recovered is constituted as a crude extract rich in glycosides. The crude extract rich in semi-concentrated glycosides can be purified in the following manner. The above glycoside extract is mixed with 200 ml. of absolute ethyl ether. The material insoluble to the ether is recovered by sedimentation, decantation or filtration and dissolved in 100 ml. of water and the solution is extracted with 100 ml. of n-butanol saturated in water, in duplicate. The butanolic phases are combined, concentrated and evaporated under vacuum. The residue is mixed in 200 ml. of ether and the residue is recovered by filtration. Example 2 Preparation of a mixture of glycosides, cotyledons of Calocarpum, Chrysophyllum or Lucuma. Take 1 kg. of fresh pulverized cotyledons degreased from Calocarpum, Chrysophyllum or L cuma and macerated in a mixture of 2 liters. of methanol / 50% water, boiling in a water bath for ½ hour. The suspension is not recovered and the residue is subjected to the previous extraction treatment for 1 more times and the suspensions thus obtained are combined and separated by hot filtration. The suspension is concentrated at a minimum volume of approximately 2 liters. The purification process is as described for example 1, the extraction being 1 lt. of n-butanol and the isolation procedure is continued. Example 3 Preparation of cotyledon glycosides of Calocarpum, Chrysophyllum or Lucuma.

The procedure is as described for example 2. Florisil® (50 g) is used, which is suspended in 100 ml. of water and the mixture is placed in a glass column with a stopcock for elution. The resin is regenerated with 100 ml. of hydrochloric acid diluted 1: 3 until the eluate maintains acidic pH. Then the resin is rinsed with methanol and then at neutral pH with 500 ml. of distilled water. Dissolve 4.7 g. of the glycoside mixture obtained in Example 2, in a mixture constituted of 30 ml. of water and 10 mi. of ethanol and then the solution is passed through the column with the resin. 300 ml are eluted, with water in 6 fractions of 50 ml. Fraction 2 contains a main spot in thin-layer chromatography, pre-coated plate (Merck-60F254), sulfuric acid-naphtorosorcinol developer. This fraction is concentrated by rotovap under vacuum and extracted with 20 ml. n-butanol; the butanolic extract, likewise, concentrates in vacuo and is restored with 5 ml. of methanol to then try to crystallize. 0.710 g is obtained. of a crystalline powder that responds to the tests of glycosides and for carboxylic acids (ref 14,56). The fraction 3 obtained from the column is monitored in a similar way to the previous one, it is concentrated, extracted and the same concentration techniques are practiced as for fraction 2. The methanol concentrate is refined, as are crystallization techniques with which yields 0.581 g. of a glycoside that responds to the tests for glycosides and amides (ref 14,56). Fraction 4 and 5 obtained from the column are monitored by the same thin layer chromatography technique, finding a compound of less polarity. This fraction is recovered by the same concentration-extraction techniques, than for the previous fractions, only it is restored with a mixture of 5 ml. of water-acetonitrile (9: 1) and crystallization techniques are practiced. You get 0.752 g. of a glycoside in the form of crystalline plates that responds to glycoside and nitrile tests (ref 14,46,56). Example 4 Preparation of genins and sapogenins from Calocarpum, Chrysophyllum or Lucuma using semi-purified exogenous enzymes. 750 g of new (immature) leaves of Hevea sp. which are crushed to a dry fine powder, frozen in acetone at -30 ° C, then placed in a buffer solution of 50 mM Tris-HCl at pH 7.0 after which they are homogenized in a mechanical blade mixer for 10 minutes. min. and squeezed through a 6-layer mesh of gauze fabric. The filtrate is centrifuged at 8,000 x g for 90 min. the resulting supernatant liquid is fractionated using gradients of (NH4) 2S04 concentration, the precipitate of 30 to 80% saturation is collected by centrifugation to the same parameters above; resuspend in the minimum volume of the previous buffer solution and dialyze against the same solution. The process of obtaining the glycosides of the "sapotes" is carried out as for example 2, but the double of "sapote" seeds is used; the procedure for its hydrolysis is done at room temperature with 250 ml. of deionized water to which the aforementioned buffer solution is added with the mixture of soluble proteins including the semi-purified Hevea glucosidases. The activity of the Hevea glycosidases towards the related glycosides related to the cyanoglycoside of the Sapotaceous family is determined at the beginning of the stage (basal), intermediate and after the incubation period which is approximately 20 to 30 min. once the degradation of the glycosides and the formation of glucose has ceased with the concomitant release of the aglycones has ended. These reactions are estimated by means of the determination on aliquots of the glucose reaction mixture, using the Merck GOD-POD kit (for more information see the insert), you can use a glucose calibration curve or evaluate when there is no change in the optical density of the series of reaction aliquots, time that is considered as the end of the reaction, using the aforementioned buffer solution plus the mixture of Hevea proteins as target. The above mixture is subjected to separation by accelerated temperatures until it reaches boiling, arranged in this device with a recovery resource; to recover the genins and sapogenins in the first fractions collected. 150 ml are recovered in a first fraction containing most of the expected extracts. The genins are separated and recovered in a separating funnel at 5 ° C as an oily fraction of the aqueous solution. The sapogenin mixtures are recovered from the aqueous fraction by extraction with 300 ml. of ether for three times, the extracts are combined and concentrated to dryness. In order to purify the sapogenins, it can be crystallized using some appropriate technique. Example 5 Preparation of genins and sapogenins from Calocarpum, Chrysophyllum or Lucuma using semi-purified enzymes of Prunus vulgaris.

It uses 600 g. of seeds of Prunus vulgaris of the same variety which are removed from the endocarp and the seed is sterilized with a 0.5% (w / v) sodium hypochlorite solution, dried with filtered air. They are stored at 4 ° C, in their homogenization, in the extraction and semipurification at the same conditions as before. The previous seeds cut in half with a scalpel in a buffer solution of 0.1 M Tris-HCl at pH 6.0 with 4 g are homogenized in a mortar. of PVP plus 6 g. of glass beads. The homogenate is filtered through 6 layers of gauze fabric and the filtrate is centrifuged for 90 min. at 8,000 x g. The supernatant is removed from the glass beads and the lipid phase. The intermediate supernatant is decanted and recentrifuged to the same parameters and dialyzed against 2 liters. of a 0.01 M Tris-HCl buffer solution at a pH of 6.0. The process of obtaining and hydrolysis of the glycosides of the "sapotes" is carried out as for example 2 and 4 respectively except that the hydrolysis is done at room temperature with deionized water to which the above mentioned buffer solution is added with the mixture of soluble proteins including the semipurified Prunus vulgaris glucosidases. The activity of the exogenous ß-glucosidases of the genus Prunus on the glycosides related to the cyanoglycoside plus the recovery of the genins and sapogenins of the Sapotaceous family of the referred genera is carried out just as for example 4. A pattern of amygdalin is used using the protein fraction extracted as a means of monitoring the enzymatic activity throughout the process of recognizing the reaction start, intermediate and termination and final isolation. The amylase hydrolase, the prunasin hydrolase, the mandeloni.tril lyase plus some isoenzyme groups or some multiple enzymatic forms that catalyze the degradation of the amygdalin to hydrogen cyanide and benzaldehyde are extracted, semipurified and recovered as a complex enzyme system in a protein complex of chemical properties and similar molecular weights by centrifugation at a determined pH of the homogenate made and extracted at 4"C of peach seeds, which give as a result three main compounds derived from the exogenous substrates present in the family Sapotácea Calocarpum (Chrysophyllum) , or Lucuma) of the related species.

Example 6 Preparation of genins and sapogenins from Calocarpum, Chrysophyllum or Lucuma using exogenous, extrageneric zymogenic plant enzymes in a buffered acidic medium. The procedure for extraction, recovery and isolation of the zymogens is as described for example 4 with the variant of the use of a homogenate of 450 g. of fresh leaves of Citrus vulgaris in a 5% acid citrate medium as a zymogenic agent. The procedure for obtaining the glycosidic substrates related to the cyanoglycoside of the "sapotes" is as for example 2. The hydrolysis process is as described for example 4 and 5 except that the two extracts are added, the zymogenic plus the substrate and homogenized at room temperature with the 5% acid citrate medium in deionized water and kept at a temperature between 35-50 ° C with agitation for 15 to 30 minutes more. The recovery of the genins and sapogenins is as for example 4. EXAMPLE 7 Use of exogenous zymogenic extract, extrageneric plant for the preparation of genins and sapogenins, taking advantage of the solid residue of the lipid extraction of the Calocarpum, Chrysophyllum or Lucuma as a substrate. 1 Kg of "degreased meal" of seeds of Lúcuma mammosum (or some other related species of the genera Calocarpum, Chrysophyllum or Lucuma) obtained as a residual solid product of the (I expression) lipid extraction (using non-polar solvents as a means of extraction, consult reference 68 for more information), as a substrate in the exogenous zymogenic reaction. As a zymogenic ferment, a fresh Citrus medica seed preparation is used, obtained in the same manner as in Example 5. A preparation of H Kg. Of a homogenate of preferably fresh seeds is used. The procedure of hydrolysis and obtaining the genins and the sapogenins is as for example 6, The substrate plus the zymogenic preparation is put in contact in 2.5 Lt of deionized water, it is homogenized at room temperature (15-40 ° C) by half an hour and the products are isolated and recovered as for example 4.

Example 8 Demonstration of the activity of "emulsins" of different exogenous, extrageneric zymogenic systems on the glycosides found in the seeds of the Calocarpum, Chrysophyllum or Lucuma treated in different ways. In this example the zimogénica fermentative activity of different sources of "emulsinas" on pulverized seeds of the Calocarpum sapota, (or related species of the Calocarpum, Chrysophyllum or Lucuma); in a fresh, dry state, in the form of degreased flour with lipid extraction, (or residual product of lipid extraction [ref 68], degreased by means of extraction with non-polar solvents or of intermediate polarity); and the lipid expression chicharrón (or residual lipid expression product [ref 68]) and the use of different alternative, exogenous, external, extrageneric enzymatic systems, such as the procedures used in examples 5,6 and 7 of this application, contrasted against the same procedures without using exogenous (external) enzymatic systems.

The two ways of obtaining the seeds of the related "sapote" such as the degreased meal of lipid extraction and the pork rind of lipid expression is as obtained in example 1 and 2 respectively of the related patent application by the same author (ref. 68), about the expression of lipids and extraction of oils from sapotes and the use of residues from these processes to obtain glycosides related to cyanoglycoside and its geninic and sapogenic derivatives. Twelve stages are made to the four stages of the pulverized seeds of the aforementioned related "sapote", practicing the procedures of examples 5, 6 and 7 of this application, of introduction of the exogenous zymogenic agents cited, in comparison with the same procedures practiced to the powdered ones of the seeds of the related "sapotes" without the introduction of external (exogenous) zymogenic agents; the first in a fresh state, the second in a dry state (more than a year of storage), the third in a state of chicharron lipid expression, and the fourth as defatted flour with lipid extraction. 1 kg are used. of the powdered seeds of the "sapote" related as ways to treat the seeds in the demonstration of the activity of exogenous "emulsins" of different extrageneric enzymatic systems for each test respectively; raw, crushed in the fresh state, in the dry state, in the form of degreased flour and in the form of chicharrón of expression, respectively, for each test. As they are done the same procedure using 1 kg. , for each test to the seeds of the "sapote" related but without the introduction of external exogenous, extrageneric zymogenic agents. Next after a time of 15 to 20 minutes it is separated as for example 4. It is quantified by the yield of sapogenins and genins. The twelve previous procedures with the introduction of the zymogenic agents through the use of alternating catalyst systems such as those used in examples 5, 6 and 7 are compared against the four processes that use only the substrate of the seeds of the "sapote" related without the use of exogenous, external, alternating plant biological catalytic systems, of "emulsins" different from those found in the pulverized ones in the test of the Calocarpum, Chrysophyllum or Lucuma of the cited species. The results are summarized in the following table.

Table of results1 of genin and sapogenin yields by exogenous extragenous vegetable zymogen ferments "emulsins", expressed in relative proportion for 1 kg of vegetable matter (pulverized). The marks indicate (-) less quantity and (+) greater relative amount. 1 For the genins, liquid extraction is carried out: liquid and quantified by volume (see example 4 of this application). The results for the sapogenins are obtained by semiquantitative techniques for the aqueous soluble mixture that is separated from the genins, an aliquot of the extract is taken and an excess of phenylhydrazine hydrochloride dissolved in aqueous sodium acetate is used (ref 14, procedure 12.9 .1f) and compared against the similarly prepared pattern using a water sample.

From the analysis of the results it is noted that the procedure that obtained the least result in the yield of sapogenins and genins, and that is used as a means of comparison, against the other procedures in seed test of the related "sapote" is the one that does not use zymogenic agents exogenous Within this group, the one that had the lowest yield was the procedure that uses the degreased seeds (without the use of alternative catalytic systems such as the zymogenic aggregates described in example 5,6 and 7 of this application) attributable to the lower biological catalytic power of this stadium of the seeds. This situation of lower yield is concluded to result from the use of organic solvents (mainly non-polar or medium polarity solvents to obtain degreased flour, which extract fats and different biochemical constituents) by denaturing the activity of the biological catalysts (ref. 56) characteristic of the Calocarpum, Chrysophyllum or Lucuma, of the related species under test; no yield is obtained in sapogenins and genins as well as for the chicharrón of expression; attributable results, in the demonstrations of the previous trials, in which no usefulness is obtained in the performance of pork rind expression to the conditions of use, attributable to the use of heat, 80-110 ° C) which likewise denaturalize the enzymes of the Calocarpum, Chrysophyllum or Lucuma, of the species related in test. Comparing the last two procedures, which already have little enzymatic activity due to the explained reasons of "denaturation" of the own catalytic activity, endogenous of the seeds of the "sapotea" thus prepared unlike the pulverized of the seeds in state fresh, which was the most obtained to these conditions (without the use of alternative catalytic systems, exogenous); followed by seeds in dry state that obtained few genins and did not obtain sapogenins at the same conditions. Contrast the application of the substrate in the form of flour degreased with solvent that did not obtain any yield in genins and in sapogenins, in the procedure without the use of an alternative, external enzymatic catalytic system, against the use of these alternative enzymatic systems, exogenous , external that give high yields in sapogenins and genins when these systems are restored from another appropriate and peculiar vegetable source, exogenous with the systems of examples 5,6 and 7 of this application. As for the other states of the seeds used that give high yields in sapogenins and genins when alternative, exogenous, external enzymatic systems of different species that give high yields are used. From the results of the procedures applied to the vegetable matter of the Calocarpum, Chrysophyllum and Lucuma in the fresh, dry states, of degreased flour and in the form of chicharrón of expression, in the which is obtained more performance with the use of alternating catalytic systems, is by using the substrate of seeds in fresh state (as applied in the procedures of examples 5,6 and 7 of this application), attributable to the greater catalytic power of the Seeds of these conditions of the Calocarpum, Chrysophyllum or Lucuma of the related species under test, in this state by the reinforcement of the activity in the transformation through the use of a second alternative biological catalyst system of another vegetable of different species , exogenous, appropriate and peculiar that increases and accelerates the transformation towards the sapogenins and genins in a given time. When there is no alternate, exogenous, extrageneric catalytic system, there is less transformation towards the sapogenins of Calocarpum, Chrysophyllum or Lucuma of the related species under test, this is because there is not enough transformation in the detection of the product., catalyzed enzymatically to the sapogenins. It is concluded that the genera Calocarpum, Chrysophyllum and Lucuma do not contain active elements in these catalytic (or non-functional) systems. It is recognized that these transformations are artificially carried out by nonspecific or specific enzymes (peculiar, exogenous glycosidases systems) towards the sapogenins, as well as the other expected gene product and that they are inclined to these two groups of substances, some acid and amide soluble, the sapogenins; other oily, aromatized and aldehyde, the genins. The results of the yields in genins and sapogenins of the other procedures in the dry state or in the state of degreased flour and as pork rinds of lipid expression are not to be impaired with the use of a catalytic alternating system of another species, such as those used in examples 5,6 or 7. It is noted that with the use of 1 kg. of dried defatted seeds involve almost twice the substrate without degreasing (fixed lipid content close to 50%) which also results in a convenience method for obtaining sapogenins and genins by using zymogenic catalytic, alternating biological, external ( exogenous) of another peculiar and suitable plant species as those described in example 4,5,6 and 7. Example 9 Test for the percutaneous activity of the sapogenins of Calocarpum, Chrysophyllum or Lucuma.

The percutaneous activity of the treatment cream to combat senescence of the skin of example 11 of the patent application related by the same author (ref 68) is verified, which uses in the demonstration of that example the uses of the sapogenins ( and genins) of the "sapotes" in cosmetic and pharmaceutical dermatological preparations for their synergistic activity with the total fixed lipids or with some fraction thereof, also of the "sapotes" that are the reason for the aforementioned patent application (ref. ), by means of the efficiency of the activity of this cream for the action of percutaneous absorption as an oil-in-water emulsion that together contains the sapogenins of Calocarpum, Chrysophyllum or Lucuma, presumed principal agent of the percutaneous activity and as adjuvants of this action to fixed lipids (ref 68, example 11) and to the genins of Calocarpum, Chrysophyllum or Lucuma in a cosmetic preparation. All the ingredients of Calocarpum, Chrysophyllum and Lucuma are according to the two inventions of the "sapotes" related by the same author. The cream also contains anionic emulsifiers and wetting agents that also contribute to the aforementioned activity. The cream is wanted to verify its efficiency as a vehicle of percutaneous absorption for hydroquinone at 0.8%, against the same cream but without sapogenin mixture. Hydroquinone serves to return pigmentation to people affected by "cloth" and spots on the skin of senescence, which requires being absorbed by the stratum corneum layers and passing to the deep layer of the epidermis to exert its effects. The treatment is given not only by the problem of the spots but also because skin care treatments are required concomitantly. The test is carried out in 4 women and 1 man from 28 to 49 years of age, who have the problem on the skin of the face due to hyperpigmentation spots or commonly called "cloth" spots. The products are applied subsequently every day for 28 days, in the affected region, applying from ½ to 1 teaspoon for tea that includes 0.3 to 0.5 gram of the cream in test. A lady and the gentleman in test receive the product # 1, consisting of the absorption vehicle plus hydroquinone, preparation of example 11 of the aforementioned related patent application (ref 68), which contains the mixture of sapogenins and fixed lipids total as used in the present exemplification and in the related patent application (ref 68), for its synergistic action comprising any form of mixtures thereof, of which hydrated lamellar lipid phases of according to the two invention of the "sapotes". Two of the remaining ladies received product # 2, consisting of the same cream but removed from the presumed main responsible ingredient of the percutaneous activity, the sapogenins of Calocarpum, Chrysop yllum or Lucuma. The fourth lady received only the excipient percutaneous but without the agent of pigmentation, hydroquinone. The results obtained at the end of the test period were of the fading of the spots more deeply and more quickly for the individuals receiving the test product # 1, which is the cream for treatment of cutaneous senescence such as that of example 11 of the application related patent (ref 68) and with the sapogenins of Calocarpum, Chrysophyllum or Luccuma, invention of this application. The individuals who received the product # 2, in the test, do not turn out as efficiently in the fading of the stain, as with the product # 1. I compare the two test groups against the lady used as control, who received the excipient percutaneous as the cream of example 11 of the related application mentioned above (ref 68), but removed from hydroquinone as effectiveness control. Example 10 Demonstration of the bacteriostatic activity of the sapogenins of Calocarpum, Chrysophyllum or Lucuma. The test is based on the bacteriostatic power of the sapogenins at concentrations of 5.0 g / 1 the "in vitro" test is performed with 100 microliters in a diffusion test with disks containing the test substance and applied on an agar plate which is planted with a standardized suspension of a microorganism. After incubation for one night, the diameters of the zones of inhibition or clearance around the discs are measured. The results of the tests "invited" of agar diffusion on the susceptibility together with other experiments demonstrate a high efficiency of the test as plant extractive matter of enzymatic algiconic hydrolyzate sapogenins, as obtained from example 7, to exert bactericidal effects. Zones of inhibition were obtained for the mixture of sapogenins 2.0 mm in diameter subsequent to incubation. After the incubation period in a culture medium with mixed axillary cultures, the zone of inhibition was 1.5 mm in diameter, resulting in the same conditions. In a liquid culture medium, it showed that only 500 mg of the dry, crude extract of enzymatic alginic acid hydrolyzate of sapogenins fromCalocarpum, from example 7, in 1 kg. of substrate were sufficient to exert bactericidal power for all germs inoculated in the development substrate, previously seeded. The same results were obtained with only 300 mg of the mixture of isolated and purified acid sapogenins of Example 6, which were added to a similar medium. Example 11 Test for the genins of Calocarpum, Chrysophyllum or Lucuma as a rubefacient agent with activity in the renewal of the epidermis. It is desired to evaluate the genomes of Calocarpum, Chrysophyllum or Lucuma as obtained from example 6 according to this invention, as a capillary rubefacient agent for which six gentlemen affected by premature alopecia are used to which the test subjects are asked for a test. weeks, which consists of applying 0.5 to 1 ml. of the products diluted in test in the affected area of the scalp with clean skin. The two gentlemen of group # 1, received the mixture of genins of Calocarpum, . { Chrysophyllum or Lucuma) in a preparation consisting of the product as obtained from example 6, 0.5% in an excipient. The composition of the excipient per 100 g. is: water 20 g., propylene glycol 25 g., sunflower oil 54.5 g. The two gentlemen of group # 2 received the same composition of the previous excipient and 0.5% resorcinol. The two gentlemen of group # 3, received only the aforementioned excipient. The following questionnaire is recorded and pertinent observations of the test are made. Age; sex; from when they present alopecia; Treatment duration; 1. - The treatment was irritating: a) It was not irritating, b) mild, c) moderate, d) high, e) severe. 2. - Type of irritation: a) hyperemia (heat), b) inflammation, c) vesication (produces blisters) 3. - Keratolytic (production of desquamation): a) No effect, b) mild, c) moderate, d) extensive. 4.- Smell: a) pleasant, b) without comment, c) unpleasant. 5. - Sensation when applying the products under test: a) Pleasant, b) without comments, c) unpleasant. 6. - Comments of the test. Primary effects perceived by the treated individual: a) Beneficial, b) adjuvants, c) no effect, d) harmful. 7. - Type of effects and observations. The study showed that the genomes of the Calocarpum, (.Chrysophyllum or Lucuma) are effective as a rubefacient for the region of the scalp of the head producing hyperemia, more tenuous than resorcinol, which is a recognized capillary rubefaciente that also produces moderate keratolysis, against the effect of the genins of Calocarpum,. { Chrysophyllum or Lucuma) that do not produce it. The genins apart present a pleasant aroma according to the survey. It is concluded that the genins of Calocarpum, Chrysophyllum or Lucuma have effects on epidermal renewal by opening the skin pore produced by hyperemia, allowing greater circulation through the epidermis of active ingredients. Example 12 Test for the activity of the sapogenins of Calocarpum, Chrysophyllum or Lucuma on the growth of the eyelashes. The test is based on a study on the activity of the products according to the invention in a determined period of time in the growth of the eyelashes, of women from 18 to 40 years of age, which show diminished growth, at that they are asked to apply the product on the eyelashes of the right eye with the eyelid tightly closed of the eye to be tested, with a small atomizer and to use the eyelashes of the left eye as a control without receiving any product. The products under test are subsequently applied for 42 days of the test, without application on the seventh day of the seven weeks of application, at a dose that is from 500 to 100 ml. The study is conducted in 8 individuals divided into two groups of four individuals each. The first group received preparations containing 0.5% of the product of example 5, mixture of seed sapogenins of Calocarpum,. { Chrysophyllum or Lucuma) in an excipient. The composition of the excipient per 100 g is: 99.5 g of injectable water. The second group received only the excipient. The results of the study are obtained as average values at the end of the test time. The analyzed values represent the development of the lashes treated against those of the untreated ones at the end of the treatment using the left eyelash of the individuals treated as the first internal control, the first group is compared against the second control group that received such treatment. only the excipient in the right eye and without treatment in the left eye. It is observed from the data obtained that the number of tabs in development status increases much faster in the group receiving the mixture of sapogenins of the Calocarpum,. { Chrysophyllum or Lucuma) according to the invention that in the group receiving the excipient, against the eyelashes of the first internal control of all the individuals in the test. It can be noted that given the growth cycle of the eyelashes, it is possible to continue with the growth phase of the eyelashes by applying longer treatments. It follows from the above observations that the duration of the growth phase of the eyelashes can be significantly prolonged and stimulate the eyelashes in their growth and in their renewal continuously with the sapogenin mixtures of the Calocarpum,. { Chrysophyllum or Lucuma) in doses as in this example without exerting pronounced side effects. Example 13 Gel for the regulation of the greasy epidermis. 0.5 of the sapogenins of Calocarpum, [Chrysophyllum or Lucuma) as obtained in Example 6, are dissolved in 50.0 of water, then the solution is gelled by the addition of 49.5 of 3% Carbomer 940. The gel is applied daily for a period of 3 months of stabilizing treatment on the skin. Example 14 Gel for the growth of the eyelashes. The following compositions are prepared: Composition A: Sapogenins from Calocarpum, [Chrysophyllum or Lucuma) as obtained from example 5. 0.4 Propylene glycol 5.0 Distilled water 82.6 Composition B Carbopol 936 1.25% gel 12.0 All components of composition A first they are mixed in the water, and the carbopol 936 anhydrous is introduced to give a gel, which can be used once or twice a day to promote the growth of the eyelashes. In this preparation the sapogenins are used as stimulating agents for hair development and as an antimicrobial preservative of the cosmetic preparation. Example 15 Treatment mascara for the promotion of eyelash growth. In this example they are included for their synergistic effect of the total fixed Lipids in conjunction with the sapogenins both of the Calocarpum, Chrysophyllum or Lucuma in the stimulation of hair development; these total fixed lipids are the subject of another patent application, see reference 68. The following composition is prepared: Sapogenins of the Calocarpum,. { Chrysophyllum or Lucuma) as obtained in Example 7. 0.5 Total fixed lipids of the Calocarpum, (Chrysophyllum or Lucuma) referred to as obtained in Example 1 of the related patent application of reference 68. 10.8 Carnauba Wax 5.0 Wax of bees 15.0 Isopropyl myristate 10.0 Stearic acid 10.0 Glyceryl monostearate 10.0 Triethanolamine 5.0 Distilled water 27.5 Sodium alignate 2.0 Black iron oxide 5.0 Example 16 Shampoo to combat hair loss with genins and sapogenins from Calocarpum, Chrysophyllum or Lucuma.

Sapogenins of the Calocarpum, (Chrysophyllum or Lucuma) as obtained in example 5. 0.8 Sodium lauryl sulfate 35.0 Monoethanolamine lauryl sulphate 20.0 Coconut diethanolamine 2.1 Citric acid 1.3 Sodium chloride 0.3 Genus of Calocarpum, (Chrysophyllum or Lucuma) as obtained of example 5. 0.5 Water 40.0 In this preparation the sapogenins are used as stimulating agents for hair development and as an antimicrobial preservative of the cosmetic preparation. Example 17 Epidermal anti-aging liposomal gel. The gel used is as prepared according to example 14, except that the distilled water in the gel preparation is replaced with a aqueous solution containing 0.4% sapogenins of the Calocarpum,. { Chrysophyllum or Lucuma) as obtained from example 6. The gel is applied to the skin, renewing and reforming where it is necessary to reduce the deterioration of the skin. Example 18 Styling gel to combat hair loss. Sapogenins from Calocarpum, Chrysophyllum or Lucuma, as obtained in example 5. 0.5 Gel at 1.5% carbomer 940 50.0 Collagen 0.2 Hydrolyzed keratin 0.1 Distilled water 49.2 BIBLIOGRAPHICAL REFERENCES 1.- Document U.S. Pat. No. 4,139,619 (1979) 6-Amino-4- (substituted amino) -1,2-dihydro-l-hydroxy-2-iminopyriminie, Topical Compositions and Process for Hair Growth. 2. - Document O.S. Pat. No. 5,723,149 (1998) Ose of Medicago Saponins for the Preparation of Cosmetic or Pharmaceutical Compositions, Especially Dermatological Compositions, Promoting Renewal of the Epidermis, Stimulating Hair Regrowth or Delaying Hair Loss. 3. - REM1NGTON Pharmacy; Gennaro A.R. editor; 19th ed. Volume 1 and 2 Editorial Medica Panamericana, (1995) p. 2418, 561, 562, 558 and 559. 4. - Morton, J .; Sapote, Canistel, Lucmo, Star Apple. In: Fruits of arm Climates, (1987) p. 397-410. http: //newcrop.hort .purdue. edu / newcrop / morton / sapote_ars .html 5. - Pouteria sapote. Pouteria sapote Acc. 199800087 URL: http: // f1ora w. eeb uconn edu / Palm eb / 199800087.html 6. - Bachstez, M., Santisteban Prieto, E. and Canales Gaja, A.M .; Studies of Lucumina, Cianogéntico Glucoside of Mamey (lucuma mammosa G.) Science (Mexico), 9, 200-202 (1948). 7. - "Free Book", anonymous author; Lost Crops of the Incas Little-Known Plants of the Andes ith Promise for Worldwide Cultivation, National Academy Press, p. 263-266 (1989). http://books.nap.edu/books/030904264x/html/ 8.- Morera, J.A .; Sapote (Pouteria sapote). In: Neglected Crops: 1492 from a different perspective. Bermejo, J.E.H. and Leon, J. (eds.) Plant Production Protection Series No. 26 FAQ, Rome, Italy. (1994) p. 103-107. 9.- The Household Cyclopedia- Distillation p. 15,16,19. http: // www. cairs.net. au /? sharefin / Cyclopedia / distilation. html 10. - Lambert, M. and Crane, J.H.; Tropical fruits. In: Advances in New Crops. Timber Press, Portland, Ed. J. Janick and J.E. Simon (1990) p. 337-355. 11. - Vetter, J., Plant Cyanogenic Glycosides. Toxicon, Pergamino, 38, 11-36 (2000). www.elsevier.com/locate/toxicon 12. - Haisman, D.R., Knight, D.J. Enzymic Hydrolysis Studies of Amygdalin, Biochem. J., 103, 528-534 (1967). 13. - Herbert, V., Laetrile: The Cult of Cyanide Promoting Poison for Profit, Am. J. Clin. Nutr., 32, 112-1158 (1979). 14.- Pasto, D.J., Johnson, C.R .; Part I. Physical Methods of Separation, Purification, and Characterization: Separation and Purification. Part III. Identification of Organic Compounds: Qualitative and Quantitative Elemental Analyzes, Functional Group Classification and Characterization In: Organic Structure Determination. Prentice-Hall, Inc., Englewood Cliffs, N.J. (1969) 15.- Takeda, T., Gonda, R., Hatano, K. Constitution of Lucumin and its Related Glycosides from Calocarpum sapota Merrill, Chemical and Pharmaceutical Bulletin, Tokyo, 45 (4), 697-699 (1997). 16.- U.S. Pat. 6,124,362 (2000) Method for Regulating Hair Growth. 17.- Winholz, M.; The Merck Index 10th ed., Rahway, N.J .: Merck and Co., Inc. (1983), p. 87, Monograph No. 620, Amigdalina. 18. - Merfort, I .; Phytochemical Study of Lucuma mammosa, Phytotherapy, 55 (4), 316-317 (1984). 19. Willuhn, G., Merfort, I., Matthiesen, U .; The Occurrence of Lanosterol and 24-Methylenelanost-8-en-3Pol in Leaves of Symphoricarpus albus. Phytochemistry 22 (1) 137-141 (1983). 20. - Alien Ph .; Poisonous and Injurious Plants of Panama. America Journal of Tropical Medicine, 23 (suppl.), 3-76 (1943). 21. - Moertel et al .; A Pharmacological Study of Amygdalin. J. Am. Assoc, 245 (6), 591-594 (1981). 22. - Winholz, M.; The Merck Index 10th ed., Rahway, N.J .: Merck and Co., Inc. (1983), p. 149, Monograph No. 1054, Benzaldehyde. 23. Bradbury, J.H., Egan, S.V., Lynch, M.J .; Analysis of Cyanide in Cassava üsing Acid Hydrolysis of Cyanogenic Glycosides. Journal of Science and Food Agriculture, 55, 277-290 (1991). 24. - Gilchrist, D.C., Lueschen, W.E., Hittle, C.N .; Revised Method for the Preparation of Standards in the Sodium Picrate Assay of HCN. Crop Science, 7, 267-288 (1967).

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Claims (21)

1. - Process for obtaining extracts of genins and sapogenins characterized in that it uses one or several derivatives or seed products or the glycosides related to cyanoglycoside in the form of an extract or in an isolated form of the Sapotaceous family and because it uses exogenous ß-glucosidase enzymes, externally added plant or microbial, other genera and sapotaceous extracts are free of toxic.

2. - Process according to claim 1 characterized in that one or several derivatives or products of the seeds of the Sapotaceae family are used from the group consisting of: a) fresh or dried seeds, whole, in pieces, ground or crushed, b) Derivative or residual solid product of seeds obtained by lipid expression. c) Derivative or residual solid product of lipid extraction or of the solid derivative of flour degreased of seeds, d) Derivatives of partial seeds or totally degreased, e) Extract or derived cyanogenic glycosidic and or one or the two remaining related glycosides.

3. - Process according to claim 1 and 2 characterized in that it consists of: a) Mix one or several derivatives of seeds of the Sapotaceous family, water and the enzymatic preparation of exogenous β-glucosidases, external to a pH of 3.0 to 7.0 at a temperature from 10 to 60 ° C, for 5 to 60 minutes, b) Separate the reaction mixture obtained from step a), at a temperature of 80 to 110 ° C for 15 to 180 minutes, or until the total amount of desired fraction, characterized by its particular fruity smell, c) Substitute the fraction of the liquid mixture obtained from the previous step to liquid extraction: liquid and separate the upper oil phase corresponding to the component of genins or benzaldehyde and separate the lower aqueous phase corresponding to the sapogenin component. d) Submit to the aqueous phase of the sapogenin component at least one liquid extraction: liquid with ethyl ether 1: 1, or using another immiscible solvent of similar polarity, separate the organic fraction and evaporate it to remove the solvent and obtain the dry sapogenin extract; it is possible to opt for fractional crystallization of the acid and amide components such as mandelic acid or its amide of the sapogenin extract by using water, methanol or ethanol, methanol / water mixtures, ethanol / water mixture and ethanol mixture / methanol / water using some suitable technique. Optionally instead of separating by boiling, the mixture obtained from step a), can be subjected to filtration on paper or gauze with cotton and the filtering liquid is recovered and followed with steps c) and d). Another treatment option of the mixture obtained from step a), is to subject it to at least one extraction to the aqueous liquid mixture using ethyl ether in a ratio of 1: 1 or 1: 2, mixing volumes: ether or by some other immiscible solvent of similar polarity, separate the ethereal phase and remove the ether by evaporation at 37 ° C or remove the solvent used alternatively, recover the concentrate and thus obtain an entire extract of sapogenins and genins.

4. - Process according to claims 1 and 2 wherein the derivative or residual solid product of seeds obtained by lipid expression is characterized in that it consists in subjecting the seeds to a pressure of 38.30 - 81.40 Newton / meter2, equal to 8 - 17 pounds / pie2 from 80 to 110 ° C, obtain the liquid oily product containing total fixed lipids to separate it and recover the residual solid product that is used to obtain the genins and sapogenins and cyanogenic and related glycosidic extract or derivatives.

5. - Residual solid product with degreased lipid expression or with remaining fat, as a residual solid derivative retained from the lipid expression of the seeds of the Sapotaceous family obtained in accordance with the process of claim 4.

6. - Process according to claims 1 and 2 wherein the solid derivative of degreased flour or the solid residue of the lipid extraction of seeds of the Sapotaceous family is characterized because it consists of: a) Optionally submit the seeds to shredding, grinding or crushing , b) Optionally squeeze as for claim 4. c) Extract with a solvent selected from methylene chloride, carbon tetrachloride, chloroform or dichloroethylene or by some other non-polar solvent or medium polarity that extracts fats, for about 24 hours, c) Filter, remove the oily liquid product containing fixed lipids by vacuum evaporation of the solvent, d) Recover the residual solid product from the previous filtration wet with solvents, dry it and grind it if necessary.

7. - Residual solid product of dry lipid extraction or as a degreased solid powder or as degreased flour, which can be obtained by the two previous subsequent degreasing processes in accordance with claims 4,5 and 6; or only for that of claim 6 or for some other degreasing treatment of seeds of the Sapotaceous family.

8. - Process for obtaining the cyanogenic glycoside and related glycosides structurally related to this, characterized by using one or several derivatives or products of the seeds of the Sapotaceous family from the group consisting of: a) Fresh or dried seeds, whole, in pieces, ground or crushed, b) Derivative or solid residual product of seeds obtained by lipid expression in accordance with claim 4 and 5. c) Derivative solid residue of lipid extraction or derivative or solid product of seeds degreased meal according to claim 6 and 7. d) Seed derivatives or fully degreased seeds according to claims 4, 5 and or 6, 7.

9. - Process for the production of the cyanogenic glycoside and structurally related glycosides related to it, derived from seeds of the Sapotaceous family according to claim 8 for alternative uses of application and or derived uses in obtaining genins and sapogenins which consists in using a the degreased flour according to claim 6 and 7 or to other derivatives or products of seeds partially or totally degreased or to the residual solid derivative of seeds obtained by lipid expression in accordance with claim 4 and 5, or to fresh or dried seeds , whole, in pieces, ground or crushed as follows: a) It is subjected to extraction with polar solvents such as water, methanol or ethanol, methanol / water mixtures, ethanol / water mixture and the extract is concentrated by vacuum evaporation, b) The primary concentrated glycoside extract from the previous step is treated with solvents of intermediate polarity such as ether et The solvent, isopropyl ether, acetone, ethylmethyl ketone or ethyl acetate and the insoluble material or the formed precipitate is recovered by sedimentation, decantation and filtration, constituting as a semi-purified extract of a mixture of structurally related glycosides related to the cyanoglycoside. c) The semipurified extract of the mixture of the above-mentioned related glycosides, the insoluble material, the precipitate and the harassing phase of the previous step is redissolved in the minimum amount of water and extracted with alcohol of low solubility in water such as n-butanol or iso-butanol that may or may not be saturated with water and the alcoholic extract is separated from the aqueous phase and concentrated by vacuum evaporation; d) The concentrate of the extract from the previous step can be chosen to be purified and the glycosidic components related to the cyanoglycoside even more using some suitable chromatographic technique, e) The extract concentrate of step c), it can also be chosen to obtain it as a concentrate of glycosides related to the cyanoglycoside in purified state for which it is dissolved in about 10 parts of its weight in water and dialysis against pure water. The previous dialysate is lyophilized or can be extracted with n-butanol and concentrated by the same technique as in step c). f) You can choose to fractionally crystallize the glycosides Related to cyanoglycoside by appropriate techniques with water, methanol, ethanol, acetonitrile aggregates or mixtures of these solvents omitting or following steps d), e) or f); or obtain it as a purified total extract.

10. - Extract of genins or benzaldehyde derived from seeds of the Sapotaceous family obtained by the process of claim 3.

11. - Extract of sapogenins or mandelic acid and its amide derived from seeds of the Sapotaceous family obtained by the process of claim 3.

12. - Extract of glycosides related to the cyanoglycoside, and of the compounds related to the own lucumin which are lucuminic acid and lucuminamide derived from seeds of the Sapotaceous family obtained by the processes of claims 8 and 9.

13. - Uses of glycoside extract related to cyanoglycoside and related compounds to lucumin itself, lucuminic acid and lucuminamide of seeds of the Sapotaceae family, obtained by the process of claim 8 and 9 for uses in obtaining genins and correlated sapogenins or for alternative use and in the use in the preparation of topical application cosmetic and pharmaceutical compositions.

14. - Uses of the genic extract and the compound of claim 10 for the preparation of topical application cosmetic and pharmaceutical compositions to act on the skin as a rubefacient, promoter of the opening of pores, as promoters of epithelial penetration.

15. - Uses of the geninic extract and the compound of claim 10 as a flavoring or flavoring additive in food and similar use substitutes.

16. - Uses of the sapogenin extract and the compounds of claim 11 for the preparation of cosmetics and pharmaceutical compositions for topical application to act on the skin as an astringent and as promoters of epithelial penetration.

17. - Uses of the sapogenin extract and of the compounds of claim 11 as a whole or in isolation for the preparation of cosmetics and pharmaceutical compositions for topical application to act as an exfoliant and with positive effects in the renewal and stimulation of the skin.

18. - Uses of the sapogenin extract and the compounds of claim 11 as a whole or in isolation for the preparation of cosmetics and pharmaceutical compositions for topical application to act as a stimulant and capillary promoter.

19. - Uses of the sapogenin extract and the compounds of claim 11 as a whole or in isolation for the preparation of cosmetics and pharmaceutical compositions or alternative compositions with bacteriostatic and fungistatic activation plus activities to microbiologically conserve preparations for topical and skin or other use .

20. - Processes according to claims 1,2,3,4,6,8 and 9 where the seeds are selected from the Sapotaceae family, preferably from the species Calocarpum sapota, C. mammosum, C. viride, Lucuma domingensis, L. salicifolia, L. obovata, L. hypoglauca, Chrysophyllum mexicanum, and Ch. Caimito.

21. - Extracts, derivatives, products or compounds according to claim 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17 and 18 in where the seeds are selected from the family Sapotaceae, preferably from the species Calocarpum sapota, C. mammosum, C. viride, Lucuma domingensis, L. salicifolia, L. obovata, L. hypoglauca, Chrysophyllum mexicanum, and Ch. caimito. Resvimen Process for obtaining extract of genins and sapogenins by enzymatic hydrolysis of seed derivatives of the Sapotaceae family, genera Calocarpum, Chrysopyllum and Lucuma. The enzyme that catalyzes hydrolysis is an exogenous β-glucosidase, of plant or microbial origin. The process can use various derivatives of seeds (seeds devoid of the endocarp) or can be cyanogenic glycoside, lucumin and related glycosides, directly. The process for the production of these glycosides starting from the derivatives of seeds and the use of these in the production of genins and sapogenins or in cosmetic, dermatological compositions is also subject to protection. The extracts of genins and sapogenins are used for similar purposes. The extracts of genins obtained are used in topical compositions to promote the opening of the pore and in the epithelial penetration or it can be used as flavoring or flavoring of food or in similar alternating uses. The sapogenin extract obtained is used in topical compositions such as astringent, exfoliating, to promote skin renewal, hair growth, retard hair loss, to combat epithelial aging and also for its bacteriostatic and fungistatic activity in its topical use or in various preparations.