KR20170074786A - Topical liniment for scalp and hair containing yeast extract derivated by saccharomyces species - Google Patents

Abstract

The present invention relates to an external application agent for scalp and hair containing Saccharomyces sp.
The saccharomyces cerevisiae decomposition extract contained in the external coating agent for scalp and hair of the present invention is a yeast of Saccharomyces sp. Which is a by-product of fermentation process of beer, By mixing and culturing, the active ingredient comprising vitamin, mineral, protein and amino acid derived from Saccharomyces cerevisiae at a high concentration and containing an active ingredient composed of alpha-lipoic acid, di-panthenol and biotin, It is possible to optimize hair growth promoting or hair loss prevention and prevention efficacy while minimizing the problem. Further, it can be applied to quasi-drugs for hair growth promotion and hair loss prevention, or cosmetic preparations for hair health promotion.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external application agent for scalp and hair containing a decomposed extract of Saccharomyces cerevisiae,

The present invention relates to an external application agent for scalp and hair containing Saccharomyces sp. Yeastolytic extract. More particularly, the present invention relates to an external application agent for Saccharomyces sp. The present invention relates to a method for promoting hair growth and promoting hair growth by containing an active ingredient composed of vitamins, minerals, proteins and amino acids derived from the Saccharomyces cerevisiae at a high concentration from a culture medium after the culture and containing an alpha lipoic acid, a di- Hair cosmetic preparation suitable for hair loss prevention or quasi-drugs for preventing hair loss, hair cosmetic preparation for scalp and hair for external use containing hairy extract of Saccharomyces cerevisiae.

The causes of hair loss have been discussed in male or female organs such as hair follicle, hair follicle, hair follicle, bacterial growth, sebaceous secretion, genetic factors and aging. Recently, it has been suggested that hair follicle cell damage caused by malnutrition of hair follicle cells and active oxygen.

However, the definite cause of hair loss has not yet been clarified. Recently, people who are suffering from alopecia are increasing and the age group is lowering.

As a conventional method for treating alopecia, there is a method of preventing activation of male hormone and increasing blood flow to hair follicle, but it is not satisfied with prevention of hair loss and hair loss.

In addition, there are hormone-related preparations based on hormone, but there are reports of skin irritation and adverse effects caused by hormone administration. Minoxidil of US-based company and trichosaccharide of Crinos Co., Ltd. are used as hair growth promoters, but there is a problem of lack of clear effect and side effects.

In addition, finasteride, developed by Merck, is known to inhibit the activity of the 5-a-reductase enzyme acting on the male hormone testosterone metabolism in hair follicles, but can cause depression, And long-term treatment is necessary.

In general, various conventional hair growth promoting methods have been proposed to promote hair growth by promoting blood circulation and nutrition of hair follicles, but this also has serious toxicity and adverse effects, and its effect is insufficient.

Therefore, there has been a demand for studies on harmless materials that can mitigate toxicity and side effects and provide efficacy.

Saccharomyces brewer's yeast strain obtained during the fermentation process of beer consists of two cell walls. The inner layer is composed of β-1.3-glucan and chitin to form skeletal polysaccharides, it can resist not only a porous network but also a significantly high mechanical impact and the outer layer has a complex structure composed mainly of mannan oligosaccharides and proteins such as? -1,6-glucan.

The cell walls of these Saccharomyces brewer's yeast strains are very hard to withstand cell sheaths above 300 herculean pressure. Since the cell wall is very hard, it has been difficult to effectively extract active ingredients such as proteins from yeast.

On the other hand, Foltene, an Italian pharmaceutical company, has introduced a female hair growth stimulant containing a component named Tricosaccaride and a component named Tricalgoxyl for men, It is reported that mineral component, specifically magnesium, promotes cell regeneration of the scalp, antioxidant function of zinc component and iron component increase blood flow to hair follicle.

As a series of reports, Korea Patent Publication No. 2010-135999 discloses a hair spray active ingredient containing essential metal ions such as amino acid and zinc, such as L-arginine, to supply nutrients to hair and scalp cells, Japanese Patent Application Laid-Open No. 2006-63088 discloses that a composition containing at least one of a mixture of vitamin A, vitamin E, vitamin C, zinc and selenium contributes to hair regrowth promotion and hair growth. And / or delayed hair loss.

Accordingly, the present inventors have been studying ways to optimize hair growth promoting or hair loss prevention and prevention efficacy while mitigating toxicity and side effect problems, and have found that various yeasts having a hard cell wall, such as Saccharomyces beer When yeast is cultivated after mixing the edible fruit juice as a natural protease, it is possible to obtain an effective ingredient as an effective ingredient in an extraction method using a protease such as water, trypsin, and protease, To 5 times higher than that of the extract of the present invention.

An object of the present invention is to provide an external application agent for scalp and hair containing Saccharomyces sp.

In order to achieve the above object, the present invention provides a method for producing a fermented beverage, which comprises extracting a yeast of Saccharomyces , a by-product of fermentation of beer, with a nutrient solution of edible fruit, And an amino acid. The present invention also provides an external application agent for scalp and hair comprising the extract of Saccharomyces cerevisiae containing the active ingredient.

In the above, the juice of the edible fruit is a juice extract obtained from any one selected from the group consisting of pears, pineapples, kiwi and papaya, and preferably a juice is used.

At this time, the content of the fruit juice of the edible fruit is 10 to 80% by weight.

In MRS celebrity busy with Mrs in the yeast Saccharomyces a saccharide used in the present invention (Saccharomyces cerevisiae), saccharose in MRS yuba room (Saccharomyces uqvarum , Saccharomyces ellipsoideus), MRS with saccharide car's Bergen sheath (Saccharomyces carlsbergensis), MRS with a MRS sake (Saccharomyces sake), saccharide saccharide Correa Taunus (Saccharomyces Mrs Lee in coreanus), poly saccharide urticae (S accharomyces lipolytica) as Saccharomyces bowl radio (Saccharomyces Mrs into boulardii) and Saccharomyces Paz Astoria Augustine (Saccharomyces Pastorianus ) is used as a mixture of two or more species.

The above-mentioned Saccharomyces cerevisiae decomposition extract contains at least one active ingredient selected from the group consisting of alpha-lipoic acid, di-panthenol and biotin extracted from the culture solution.

The above-mentioned Saccharomyces cerevisiae decomposition extract is contained in an amount of 10 to 30% by weight with respect to the whole composition to constitute an external application agent for scalp and hair.

The external application agent for scalp and hair of the present invention is applied to quasi-drugs having hair growth promoting function and hair loss preventing function selected from the group consisting of hair growth promoting agent, hair loss preventing agent, dandruff treatment agent, hair growth agent and dyes.

In addition, the external application agent for scalp and hair of the present invention can be applied to any one hair health promoting cosmetic selected from the group consisting of shampoos, treatments, essences, and hair sprays.

The external application agent for scalp and hair of the present invention can be obtained by mixing a juice of edible fruit as a natural protease in Saccharomyces sp. Yeast, which is a byproduct of fermentation process of beer, As a constitution of the Saccharomyces cerevisiae decomposition extract which contains a high concentration of the component.

Thus, the extract of Saccharomyces cerevisiae can be obtained by extracting an effective ingredient containing vitamins, minerals, proteins and amino acids derived from Saccharomyces genus dry yeast with a conventional protease or an extract using a protease such as trypsin or protease By allowing the nutrient supply to the scalp and hair at a concentration 1.2 to 5 times higher than that of the method, nutritional deficiency of the hair follicle cell, which is the cause of hair loss recently, can be improved.

In addition, the Saccharomyces cerevisiae decomposition extract may contain an active ingredient of alpha-lipoic acid as an antioxidative active ingredient and biotin and di-panthenol as an active ingredient for the treatment and prevention of hair loss from the mixed culture solution to promote hair growth, prevent hair growth and hair loss, It is useful not only for prevention but also for prevention of skin damage by active oxygen.

Thus, the extract of Saccharomyces cerevisiae can be extracted without chemical substances in the pre-extraction step. Therefore, the external application agent for scalp and hair, which comprises the composition, can optimize the efficacy while minimizing toxicity and side effects.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a photograph taken before application of an external application agent for scalp and hair of the present invention to a subject,
Fig. 2 is a photograph taken after passage of 2 months after application of the external application agent for scalp and hair of the present invention to a subject,
Fig. 3 is a photograph taken after a lapse of 2.5 months from the application of the external application agent for scalp and hair of the present invention to a subject,
FIG. 4 is a picture (Day 0) on the day of application as a result of photographing of the hair growth effect on the external coating agent for scalp and hair according to the present invention,
FIG. 5 shows the result of photographing at the time point 7 (day 7) after the application of FIG. 4,
FIG. 6 shows the result of photographing at the 14th day and the 14th day after the application of FIG.

Hereinafter, the present invention will be described in detail.

The present invention relates to a method for producing a saccharomyces cerevisiae strain, which comprises extracting a yeast of Saccharomyces , which is a by-product of fermentation of beer, with a nutrient solution of edible fruit and then cultivating the same to obtain an efficacy comprising vitamins, minerals, proteins and amino acids derived from Saccharomyces cerevisiae And an external application coating agent for scalp and hair comprising the decomposition extract of Saccharomyces sp.

That is, the external application agent for scalp and hair of the present invention is a yeast extract of Saccharomyces sp. Containing a high concentration of active ingredient extracted from Saccharomyces sp. Yeast, which is a by-product of fermentation process of beer, As a constitution.

At this time, the Saccharomyces cerevisiae decomposition extract can be obtained from the above-mentioned Saccharomyces cerevisiae by using the juice extracted from the edible fruit as a natural protease in place of the conventional protease such as water or artificial trypsin or protease. The active ingredient consisting of the derived vitamins, minerals, proteins and amino acids is contained at a concentration 1.2 to 5 times higher than the conventional extraction method [Table 1].

Particularly, compared to the conventional extraction method using protease of water or trypsin, it was found that the amount of the enzyme was twice that of iron, 1.6 times of magnesium, 1.2 times of potassium, 2.5 times of zinc, 5 times of copper, 3.3 times of protein, 3.5 times of asparagine, 1.2 times of leucine , And valine 1.3 times higher. These ingredients will nourish hair and scalp cells and keep scalp cells healthy, which will contribute to hair growth, hair growth and hair loss prevention.

In the present invention, the edible fruit root which can be used as a natural protease is any one selected from the group consisting of pears, pineapples, kiwi and papaya.

As a natural protease contained in the edible fruit, the pear contains protease, the pineapple contains bromelain, the kiwi contains actinidin, and the papaya contains a large amount of papain.

In the embodiment of the present invention, as a fruit juice of edible fruit, a preferred example is a juice, but it is not limited to this. When juice is used, consumption of domestic produce and expansion of application fields can be expected.

In this case, the content of the fruit juice of the edible fruit is preferably 10 to 80% by weight. When the content is less than 10% by weight, the cell wall breaking effect of the Saccharomyces sp. If the weight percentage is exceeded, the extract may become turbid due to an increase in the amount of water added to the extract, which lowers the product preference and is difficult to test the product.

In addition, Saccharomyces cerevisiae , which is a by-product of beer fermentation in the embodiment of the present invention, is described as an example of yeast of Saccharomyces sp., But is not limited thereto.

Thus, the yeast of the genus Saccharomyces used in the present invention includes Saccharomyces cerevisiae In addition cerevisiae), saccharose in MRS yuba room (Saccharomyces uqvarum), MRS with saccharide ellipsis Soy Amadeus (Saccharomyces ellipsoideus), MRS with saccharide car's Bergen sheath (Saccharomyces to carlsbergensis), Mrs. sake as Saccharomyces (Saccharomyces sake), Saccharomyces Correa Augustine (Saccharomyces coreanus , S accharomyces lipolytica , Saccharomyces boulardii , and Saccharomyces pastorianus may be used in combination with one or more species selected from the group consisting of Saccharomyces boulardii and Saccharomyces pastorianus .

Further, the Saccharomyces cerevisiae decomposition extract of the present invention can be obtained by culturing a mixture of a fruit juice of an edible fruit and a nutrient solution of a group consisting of a-lipoic acid, D-panthenol and biotin ≪ / RTI > [ Table 2 ].

Partial detection of alpha-lipoic acid, known as an antioxidant in the active ingredient, and di-panthenol and biotin components known as active ingredients for hair loss treatment and prevention are detected in all cases of pineapple, kiwi and pear.

Accordingly, the external application agent for scalp and hair of the present invention, which comprises the decomposition extract of Saccharomyces cerevisiae, can optimize hair growth promoting or hair loss prevention and prevention efficacy while minimizing toxicity and side effect problems.

At this time, the extract of Saccharomyces cerevisiae is contained in an amount of 10 to 30% by weight with respect to the total composition. When the content is less than 10% by weight, there are many individual differences in hair growth and hair loss prevention effect, , Or it is difficult to proceed to real hair at a fluffy level. When it exceeds 20% by weight, hair is undesirable because it becomes too stiff.

In the external application agent for scalp and hair of the present invention, other constituents other than the above-mentioned Saccharomyces cerevisiae decomposition extract may include oils. At this time, the oil flow is not particularly limited as long as it is a transparent liquid oil of low irritation, high humidity, which is used in medicine and cosmetics applied to the skin.

Examples of such oils include aragans, jojoba, coconut oil, palm oil, olive oil, grape seed oil, sunflower oil, avocado oil, hazelnut oil, evening primrose oil, rosehip oil, rice bran oil, rice bran oil, sweet almond oil, Soybean oil, shea butter, mango butter, cocoa butter, waxy oil, corn oil, sesame oil, top oil, sesame oil, apricot seed oil, emu oil, kukui nut oil, safflower oil, At least one vegetable oil selected from the group consisting of palm kernel oil; Or at least one natural herbal aroma oil selected from the group consisting of lemon, grapefruit, orange, peppermint, thyme, rosemary, cedarwood, tea tree, cinnamon and lavender; Or a mixed form thereof can be used.

1 is jeonyigo applying the external-use coating agent of the scalp and the hair of the present invention to a subject, Fig. 2 is a picture taken after 2 months after the application of the external-use coating agent of the scalp and the hair of the present invention to a subject, Figure 3 is 2.5 months As the photograph taken after the lapse of time, the number of hairs in the scalp portion increases and the hair growth with a certain length can be confirmed as the elapsed time of application time increases.

In addition, the handle 4 to the test group treated with the composition of the present invention using a C57BL / 6 mice to evaluate the hair growth effects of the external-use coating agent of the scalp and the hair of the present invention with reference to FIG. 6, the commercial product, 5% minoxidil And the control group 2 treated with physiological saline was evaluated for the induction effect of the growth factors according to the elapsed time.

5 is a photograph taken at day 7 of the experiment (Day 7), and FIG. 6 is a photograph taken at the day 14 of the experiment (Day 14), showing the test group treated with the composition of the present invention Can mimic the hair growth effect similar to 5% minoxidil, a commercial product that has proved effective in hair growth.

Accordingly, the external application agent for scalp and hair according to the present invention can be applied to any one of formulations selected from the group consisting of hair extract, hair loss preventing agent, dandruff treatment agent, hair growth agent and dye It is possible to provide a quasi-drug useful for preventing hair growth or preventing hair loss and preventing hair loss.

In addition, the external coating agent for scalp and hair of the present invention comprises a saccharomyces cerevisiae decomposition extract to provide shampoos, treatments, essences, and hair sprays that can maintain or enhance hair health by supplying nutrition to hair The present invention provides a cosmetic for enhancing hair health applied to any one of the formulations.

Hereinafter, the present invention will be described in more detail with reference to Examples.

The present invention is intended to more specifically illustrate the present invention, and the scope of the present invention is not limited to these embodiments.

< Example  1 to 4> Saccharomyces  Production of Yeast Decomposition Extract 1 to 4

1. Juice production

Only the seed portion was removed from the edible pear (550g) prepared by wiping cleanly, and the pulp containing the skin was put into a juicer to prepare a juice extracted from the juice. The juice was centrifuged at 2200 G for 30 minutes and only the separated supernatant was collected to prepare the next step.

2. Culture with juice and dried beer yeast

40 ml of the juice prepared in the above Step 1 and 10 ml of Saccharomyces cerevisiae, a dry yeast yeast strain , were mixed. The juice and brewer's yeast were mixed well and shaken and incubated at 43 ° C for 15 minutes (Example 1), 30 minutes (Example 2), 45 minutes (Example 3) and 60 minutes (Example 4).

3. Post-culture treatment

During the above-mentioned step, the culture medium was periodically mixed well during the culturing. After the culture was completed, each culture was centrifuged at 2,200 G for 30 minutes, and the centrifuged supernatant was collected and filtered through a 70 쨉 m cell strainer (Falcon 352350).

The filtered solution was again centrifuged at 10,000 G for 5 minutes, and then only centrifuged supernatant was collected.

< Example  5> Saccharomyces  Preparation of yeast degradation extract 5

Except that Saccharomyces uvarum was used as the dry beer yeast in the step of culturing the juice of step 2 of Example 1 and the dry brewer's yeast and the yeast was cultured at 43 캜 for 30 minutes. The procedure of Example 1 was repeated.

< Example  6> Saccharomyces  Preparation of yeast extract 6

In step 1 of Example 1, the pineapple was used instead of the edible pear to prepare juice. In step 2, 10 ml of juice and 30 ml of water were mixed with 10 ml of dry brewer's yeast and incubated at 43 ° C for 30 minutes The procedure of Example 1 was otherwise repeated.

< Example  7> Saccharomyces  Production of Yeast Decomposition Extract 7

In step 1 of Example 1, the juice was prepared by using kiwi instead of the edible pear. In step 2, 10 ml of juice and 30 ml of water were mixed with 10 ml of dry beer yeast, and cultured at 43 ° C for 30 minutes The procedure of Example 1 was otherwise repeated.

< Comparative Example  1>

The procedure of Example 1 was repeated except that 10 ml of dry beer yeast was mixed with 40 ml of water instead of the juice in Step 2 of Example 1, and the mixture was incubated at 43 캜 for 30 minutes.

< Comparative Example  2>

In Step 2 of Example 1, 40 ml of water and 10 ml of dry beer yeast were mixed instead of the juice, 1 ml of 0.25% trypsin EDTA was added to the mixture, and the mixture was incubated at 43 캜 for 30 minutes The procedure of Example 1 was otherwise repeated. At this time, trypsin was commercial product (invitrogen gibco).

< Experimental Example  1> Active ingredient analysis 1

In the above Examples 1 to 4 and Comparative Examples 1 and 2, 4 ml of the supernatant obtained after centrifugation and centrifugation were taken to analyze vitamins, minerals, proteins and amino acid components. The results are shown in Table 1 below.

As shown in Table 1, Saccharomyces cerevisiae, a dry brewer's yeast strain , was mixed with juices and incubated for 15 minutes, 30 minutes, 45 minutes, and 60 minutes for saccharomyces cerevisiae The extracts of Comparative Examples 1 and 2 were found to have a concentration of at least 1.2 to at most 5 times higher than that of the active ingredient containing contrast, vitamins, minerals, proteins and amino acids.

Specifically, vitamin B1, vitamin B6, folic acid, vitamin E, and vitamin C components were contained as vitamins. In contrast to conventional extraction methods using water or trypsin protease, 1.6 times of magnesium, 1.2 times of potassium, 2.5 times of zinc, and 5 times of copper.

In addition, the total content of protein is 3.3 times higher than that of conventional methods using protease of trypsin or trypsin. The amino acid components include alanine, arginine, aspartic acid, glycine, histidine, tyrosine, glutamic acid, valine, Phenylalanine, and cystine. In particular, it is contained at a high concentration of aspartic acid 3.5-fold, leucine 1.2-fold, and valine 1.3-fold compared to the conventional extraction method using water or trypsin protease .

From the above results, it is possible to optimize the culturing conditions at a culture temperature of 43 캜 and a cultivation time of 30 minutes to 60 minutes, preferably 30 minutes and 45 minutes, of the Saccharomyces cerevisiae decomposition extract.

< Experimental Example  2> Active ingredient analysis 2

As a result of the analysis of the active ingredients derived from the yeast of Saccharomyces genus in Experimental Example 1, the pineapple juice and kiwi juice of Examples 6 and 7, which were the same as those of Example 2, (D-panthenol), biotin, and a-lipoic acid were obtained from each culture broth by BioPavlla, a specialized protein analysis organization, and quantitatively analyzed Respectively.

Since the test equipments contain very many components in the juice, it is difficult to analyze them. Therefore, the highest resolution chromatograph (Acquity H class from UPLC, Waters) was used and analyzed using a centrifugal concentrator (Modulspin 40 from Hanil).

Particularly, in order to solve the problem of difficulty in reading due to a large number of peaks due to a lot of hydrophilic substances in juice, D-panthenol, biotin, a-lipoic acid component .

At this time, di-panthenol was not adsorbed on C18, and biotin and alpha-lipoic acid components were adsorbed and eluted from 50% acetonitrile. The biotin and alpha - lipoic acid were analyzed by C18 pretreatment or concentration.

In the sample pretreatment step, 50 mg of a C 18 adsorbent was packed in a Spin-X filter to make a mini-column, and the acetonitrile and the mini-column were equilibrated in the third order. One milliliter of each sample was added to the mini-column, centrifuged at 22.times.g for 2 minutes, 0.5 milliliter of tertiary order was added, and centrifugation at 22.times.g for 1 minute was repeated three times to remove the hydrophilic substance. The mini-column is then transferred to a new microcentrifuge tube and 0.5 ml of 50% acetonitrile is added. After centrifugation at 22 xg for 1 min, the eluate was collected and concentrated in a centrifuge concentrator.

The analytical conditions were as follows: 2 μl of the pretreated or untreated sample was analyzed by injection into UPLC and separated by a reverse phase chromatography method on a BEH C18 column for 10 minutes by acetonitrile gradient. At this time, the column temperature was 40 ° C, the flow rate was 0.4 ml / min, and the detection was carried out under the condition of absorbance at 201 nm, and the results are shown in Table 2 below.

From the results of Table 2, when extracted in the dried beer yeast Saccharomyces three Levy cyano (Saccharomyces cereuisiae) water, de-activation of D-panthenol (D-panthenol), biotin (biotin), alpha-lipoic acid (a-lipoic acid) No component was detected.

In addition, in the case of the culture solution in which trypsin, which is a conventional protease, was used in place of the above-mentioned water, a small amount of di-panthenol was detected, but active components of biotin and alpha-lipoic acid were not detected.

On the other hand, in the case of the example using juice instead of trypsin as the water and proteinase, a small amount of alpha-lipoic acid was present and di-panthenol and biotin components were detected in all cases of pineapple, kiwi and pear.

Particularly, the di-panthenol component was also detected in Comparative Examples 1 and 2 using water and trypsin, and therefore, it was confirmed that a large amount of di-panthenol component resulted from the use of pineapple and kiwifruit caused by Saccharomyces cerevisiae.

On the other hand, the biotin and alpha-lipoic acid components which were not detected even in Comparative Examples 1 and 2 using water and trypsin were detected in the case of using the juice of the examples, so that biotin can be derived from fruit juice, Value can be increased.

In addition, the di-panthenol and biotin components known as active ingredients for hair loss treatment and prevention are useful for external application agents for scalp and hair of the present invention.

< Experimental Example  3>

A composition containing 10% by weight of the Saccharomyces cerevisiae decomposition extract prepared in Example 2 was prepared and applied to a 60-year-old male subject having a hair loss area of about 7 cm in diameter on the head.

FIG. 1 is a photograph taken before application of the external application agent for scalp and hair of the present invention to a subject, FIG. 2 is a photograph image taken after 2 months after application, FIG. 3 is a photograph taken after 2.5 months, As the application time elapsed, the number of hairs in the scalp area increased and hair growth with a certain length of hair was confirmed.

< Experimental Example  4>

A composition containing 10% by weight of the extract of Saccharomyces cerevisiae prepared in Example 2 and a natural herbal aromatic oil having an antioxidative effect was prepared. To confirm the hair growth effect, C57BL / 6 mice were used to induce a growth- Respectively.

Six litters of C57BL / 6 mice at 6 weeks of age were sacrificed. The test group treated with the composition of the present invention, the comparative test group 1 treated with commercial product 5% minoxidil, and the comparative test group 2 treated with physiological saline Once a day, 200 ml was applied and hair growth was confirmed. Three observers who were not participating in the experiment were blinded to the area (%) of the area where the skin turned black in proportion to the area of the nipple.

The results of the 7th, 11th, and 17th days after the coating treatment were compared with the unpaired t-test in Table 3 below.

From the results of the above Table 3, there was no difference between the test group 2 and the comparative test group 2 (physiological saline treatment) at 47 days, 7 days after application, 47.47 + 27.96 vs 31.00 + 28.16 (p value = 0.105) (P value = 0.0027), and 91.63 + 7.10 vs 75.05 + 27.69 (p value = 0.026) at the 17th day of the study, the results of the test group of the present invention compared to the physiological saline treated test group were 69.19 + 19.23 vs 46.38 + 28.63 The effect was remarkably improved.

On the other hand, the comparative test group 1 treated with 5% minoxidil showed a remarkable effect when compared with the comparative test group 2 treated with physiological saline at 7, 11 and 17 days after application, The treated test group showed an excellent hair growth effect equal to or higher than that of the comparative test group 1 treated with 5% minoxidil at 17 days after application.

FIG. 4 shows the results of photographs taken on the day of application (Day 0), FIG. 5 shows the results of Day 7 (Day 7) of the experiment, and FIG. And FIG. 6 is a photograph taken at the time of day 14 (Day 14) of the experiment.

At the end of the 14 days after the coating experiment, the test group treated with the composition of the present invention visually confirmed similar effects to 5% minoxidil, which is a commercial product with proven hair growth effect.

As described above, the external application agent for scalp and hair of the present invention comprises the extract of Saccharomyces cerevisiae, and the extract of Saccharomyces cerevisiae is added to Saccharomyces cerevisiae, the juice of the edible fruit By culturing after mixing, it is possible to contain active ingredients such as vitamins, minerals, proteins and amino acids derived from Saccharomyces cerevisiae at a high concentration. Thus, the extract of the present invention can supply nutrients to the scalp and hair at a high concentration, and thus it is possible to ameliorate the malnutrition of the hair follicle, which is the cause of hair loss recently.

In particular, the extract of Saccharomyces cerevisiae can optimize its efficacy while minimizing toxicity and side effects by containing an active ingredient of biotin and di-panthenol as active ingredients for alpha lipoic acid, hair loss treatment and prevention of antioxidant activity .

Further, the external application agent for scalp and hair containing the decomposed extract of Saccharomyces cerevisiae according to the present invention may be any one selected from the group consisting of hair growth promoting agent, hair loss inhibitor, dandruff treatment agent, hair growth agent and dye, Quasi-drugs for prevention; Or cosmetics for hair health enhancement selected from the group consisting of shampoos, treatments, essences, and hair sprays.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (9)

A yeast of the genus Saccharomyces , which is a by-product of fermentation of beer, is extracted from the culture solution obtained by mixing the fruit juice of the edible fruit,
An external application agent for scalp and hair comprising a saccharomyces cerevisiae decomposition extract containing an active ingredient consisting of vitamins, minerals, proteins and amino acids derived from Saccharomyces cerevisiae. The external application agent for scalp and hair according to claim 1, wherein the fruit juice of the edible fruit is a juice extract obtained from any one selected from the group consisting of pears, pineapples, kiwi and papaya. The external application agent for scalp and hair according to claim 1, wherein the juice is a juice. The external application agent for scalp and hair according to claim 1, wherein the fruit juice of the edible fruit is mixed in an amount of 10 to 80% by weight. The method of claim 1, wherein the yeast in the MRS to the Saccharomyces celebrity busy as Saccharomyces (Saccharomyces cerevisiae), saccharose in MRS yuba room (Saccharomyces uqvarum , Saccharomyces ellipsoideus), MRS with saccharide car's Bergen sheath (Saccharomyces carlsbergensis), MRS with a MRS sake (Saccharomyces sake), saccharide saccharide Correa Taunus (Saccharomyces Mrs Lee in coreanus), poly saccharide urticae (S accharomyces lipolytica) as Saccharomyces bowl radio (Saccharomyces Mrs into boulardii) and Saccharomyces Paz Astoria Augustine (Saccharomyces wherein the external coating agent is a mixed type of one or more selected from the group consisting of pastorin , The external application agent for scalp and hair according to claim 1, wherein at least one active ingredient selected from the group consisting of alpha-lipoic acid, di-panthenol and biotin is extracted from the culture solution. [Claim 2] The external application agent for scalp and hair according to claim 1, wherein the extract of Saccharomyces cerevisiae comprises 10 to 30% by weight of the total composition. The hair cosmetic composition according to claim 1, wherein the external application agent for scalp and hair is a quasi-drug for hair growth promotion and hair loss prevention applied to any one of formulations selected from the group consisting of hair growth promoters, hair loss inhibitors, dandruff remedies, hair growth agents, External application agent for scalp and hair. The external-use cosmetic for hair and scalp according to claim 1, wherein the external-use coating agent for scalp and hair is a cosmetic for enhancing hair health applied to any of formulations selected from the group consisting of shampoos, treatments, essences and hairspray liniment.

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