MXPA04004691A - Metodo para preparar materiales biologicos para la criopreservacion utilizando sustancias protectoras preenfriadas. - Google Patents
Metodo para preparar materiales biologicos para la criopreservacion utilizando sustancias protectoras preenfriadas.Info
- Publication number
- MXPA04004691A MXPA04004691A MXPA04004691A MXPA04004691A MXPA04004691A MX PA04004691 A MXPA04004691 A MX PA04004691A MX PA04004691 A MXPA04004691 A MX PA04004691A MX PA04004691 A MXPA04004691 A MX PA04004691A MX PA04004691 A MXPA04004691 A MX PA04004691A
- Authority
- MX
- Mexico
- Prior art keywords
- protectant
- freezing
- biological material
- thawed
- biologically active
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Thermotherapy And Cooling Therapy Devices (AREA)
Abstract
Se presenta un metodo para preparar materiales biologicos para criopreservacion. El metodo reduce la cantidad de calor liberado por un crioprotector durante una fase de calor latente cuando se congela el protector, se descongela el protector y se tratan los materiales con actividad biologica con las sustancias protectoras descongeladas. En primer lugar, se congela la sustancia protectora para inducir un cambio de fases irreversible junto con una liberacion de energia irreversible. Despues de que ha ocurrido este cambio de fases, se descongela la sustancia protectora y se utiliza para tratar las celulas viables u otro material con actividad biologica que vaya a ser sometido a congelacion. La sustancia protectora descongelada dentro de las celulas con actividad biologica tiene una reaccion endotermica reducida tras el congelamiento ulterior. En una modalidad, el congelamiento del material biologico consiste en sumergir el material biologico en un liquido refrigerante y hacer circular el liquido refrigerante sobre el material biologico a una velocidad predeterminada, considerablemente constante y una temperatura tal que se vitrifique el material biologico, reduciendo asi al minimo la formacion de fracturas por esfuerzos en las membranas celulares. La sustancia protectora puede ser propilen glicol, glicerol, DMSO u otras sustancias protectoras convenientes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/989,715 US20030096412A1 (en) | 2001-11-20 | 2001-11-20 | Method of preparing biological materials for cryopreservation using pre-chilled protectant |
| PCT/US2002/037325 WO2003051111A2 (en) | 2001-11-20 | 2002-11-20 | Cryopreservation of biological materials using pre-chilled protectant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA04004691A true MXPA04004691A (es) | 2004-08-19 |
Family
ID=25535401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA04004691A MXPA04004691A (es) | 2001-11-20 | 2002-11-20 | Metodo para preparar materiales biologicos para la criopreservacion utilizando sustancias protectoras preenfriadas. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20030096412A1 (es) |
| EP (1) | EP1446005A2 (es) |
| JP (1) | JP2005511756A (es) |
| KR (1) | KR20040088469A (es) |
| CN (1) | CN1655672A (es) |
| AU (1) | AU2002359438A1 (es) |
| CA (1) | CA2467638A1 (es) |
| IL (1) | IL162055A0 (es) |
| MX (1) | MXPA04004691A (es) |
| NO (1) | NO20042598L (es) |
| RU (1) | RU2004118507A (es) |
| WO (1) | WO2003051111A2 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120051737A (ko) * | 2009-08-19 | 2012-05-22 | 다카라 바이오 가부시키가이샤 | 세포의 보존 방법 |
| CN102197802B (zh) * | 2011-03-25 | 2013-01-16 | 中国人民解放军南京军区福州总医院 | 一种胰岛冻存保护剂及其使用方法 |
| CN111455562A (zh) * | 2019-01-18 | 2020-07-28 | 南京大学 | 一种导热纤维膜的制备方法及其应用 |
| CN115561443B (zh) * | 2022-11-17 | 2023-03-07 | 云贺生物科技(南京)有限公司 | 活性检测数据处理系统及方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244864A (en) * | 1988-07-07 | 1993-09-14 | Great Lakes Chemical Corp. | Methods for protection and treatment of plants exposed to chilling temperatures |
| US4840035A (en) * | 1988-07-14 | 1989-06-20 | Barnet L. Liberman | Method of freezing tissue |
| US5856081A (en) * | 1991-07-08 | 1999-01-05 | The American National Red Cross | Computer controlled cryoprotectant perfusion apparatus |
| US5518878A (en) * | 1993-09-15 | 1996-05-21 | Organogenesis Inc. | Cryopreservation of cultured skin or cornea equivalents with agitation |
| CZ20031811A3 (cs) * | 2001-01-02 | 2004-02-18 | Supachill Technologies Pty. Ltd. | Způsob přípravy vzorků tkání pro histologické a patalogické zkoumání a soustava pro provádění způsobu |
| US6803227B2 (en) * | 2001-10-16 | 2004-10-12 | Supachill Technologies Pty. Ltd. | Organ preservation system including articles comprising a super-coolable composition having long-duration phase change capability |
| US6656380B2 (en) * | 2001-10-16 | 2003-12-02 | Supachill Technologies Pty. Ltd. | Super-coolable composition having long-duration phase change capability, process for preparation of same, process for super-cooling same and articles comprising same |
| US6681581B2 (en) * | 2001-11-20 | 2004-01-27 | Supachill Technologies Pty. Ltd. | Pre-conditioned solute for use in cryogenic processes |
-
2001
- 2001-11-20 US US09/989,715 patent/US20030096412A1/en not_active Abandoned
-
2002
- 2002-11-20 WO PCT/US2002/037325 patent/WO2003051111A2/en not_active Application Discontinuation
- 2002-11-20 KR KR10-2004-7007738A patent/KR20040088469A/ko not_active Application Discontinuation
- 2002-11-20 CN CNA028273001A patent/CN1655672A/zh active Pending
- 2002-11-20 IL IL16205502A patent/IL162055A0/xx unknown
- 2002-11-20 RU RU2004118507/15A patent/RU2004118507A/ru not_active Application Discontinuation
- 2002-11-20 JP JP2003552050A patent/JP2005511756A/ja active Pending
- 2002-11-20 MX MXPA04004691A patent/MXPA04004691A/es unknown
- 2002-11-20 AU AU2002359438A patent/AU2002359438A1/en not_active Abandoned
- 2002-11-20 EP EP02793978A patent/EP1446005A2/en not_active Withdrawn
- 2002-11-20 CA CA002467638A patent/CA2467638A1/en not_active Abandoned
-
2004
- 2004-06-21 NO NO20042598A patent/NO20042598L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| IL162055A0 (en) | 2005-11-20 |
| US20030096412A1 (en) | 2003-05-22 |
| KR20040088469A (ko) | 2004-10-16 |
| NO20042598L (no) | 2004-08-20 |
| WO2003051111A2 (en) | 2003-06-26 |
| CA2467638A1 (en) | 2003-06-26 |
| CN1655672A (zh) | 2005-08-17 |
| AU2002359438A1 (en) | 2003-06-30 |
| RU2004118507A (ru) | 2005-04-10 |
| JP2005511756A (ja) | 2005-04-28 |
| EP1446005A2 (en) | 2004-08-18 |
| WO2003051111A3 (en) | 2003-10-09 |
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