MXPA02012818A - Vacunas de virus sincitales respiratoriso que expresan antigenos protectores a partir de genes proximos promotores. - Google Patents
Vacunas de virus sincitales respiratoriso que expresan antigenos protectores a partir de genes proximos promotores.Info
- Publication number
- MXPA02012818A MXPA02012818A MXPA02012818A MXPA02012818A MXPA02012818A MX PA02012818 A MXPA02012818 A MX PA02012818A MX PA02012818 A MXPA02012818 A MX PA02012818A MX PA02012818 A MXPA02012818 A MX PA02012818A MX PA02012818 A MXPA02012818 A MX PA02012818A
- Authority
- MX
- Mexico
- Prior art keywords
- rsv
- genome
- antigenome
- gene
- genes
- Prior art date
Links
- 241000725643 Respiratory syncytial virus Species 0.000 title claims abstract description 1192
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 892
- 229960005486 vaccine Drugs 0.000 title claims abstract description 126
- 230000001681 protective effect Effects 0.000 title claims description 25
- 239000000427 antigen Substances 0.000 title description 21
- 108091007433 antigens Proteins 0.000 title description 21
- 102000036639 antigens Human genes 0.000 title description 21
- 241000700605 Viruses Species 0.000 claims abstract description 276
- 208000015181 infectious disease Diseases 0.000 claims abstract description 214
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 206
- 239000002157 polynucleotide Substances 0.000 claims abstract description 206
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 206
- 230000035772 mutation Effects 0.000 claims abstract description 187
- 230000002458 infectious effect Effects 0.000 claims abstract description 179
- 239000002773 nucleotide Substances 0.000 claims abstract description 147
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 146
- 230000002238 attenuated effect Effects 0.000 claims abstract description 139
- 108090000288 Glycoproteins Proteins 0.000 claims abstract description 107
- 102000003886 Glycoproteins Human genes 0.000 claims abstract description 92
- 238000012217 deletion Methods 0.000 claims abstract description 90
- 230000037430 deletion Effects 0.000 claims abstract description 90
- 230000014509 gene expression Effects 0.000 claims abstract description 62
- 239000013598 vector Substances 0.000 claims abstract description 49
- 230000004048 modification Effects 0.000 claims abstract description 48
- 238000012986 modification Methods 0.000 claims abstract description 48
- 230000028993 immune response Effects 0.000 claims abstract description 46
- 238000003780 insertion Methods 0.000 claims abstract description 44
- 230000037431 insertion Effects 0.000 claims abstract description 43
- 239000000203 mixture Substances 0.000 claims abstract description 39
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 39
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 35
- 230000000694 effects Effects 0.000 claims abstract description 26
- 230000008707 rearrangement Effects 0.000 claims abstract description 17
- 230000007423 decrease Effects 0.000 claims abstract description 10
- 238000009472 formulation Methods 0.000 claims abstract description 8
- 241000124008 Mammalia Species 0.000 claims abstract description 7
- 101710159752 Poly(3-hydroxyalkanoate) polymerase subunit PhaE Proteins 0.000 claims abstract 13
- 101710130262 Probable Vpr-like protein Proteins 0.000 claims abstract 13
- 101000833492 Homo sapiens Jouberin Proteins 0.000 claims abstract 3
- 101000651236 Homo sapiens NCK-interacting protein with SH3 domain Proteins 0.000 claims abstract 3
- 102100024407 Jouberin Human genes 0.000 claims abstract 3
- 230000008859 change Effects 0.000 claims description 206
- 241000711920 Human orthopneumovirus Species 0.000 claims description 163
- 102000004169 proteins and genes Human genes 0.000 claims description 114
- 101710118188 DNA-binding protein HU-alpha Proteins 0.000 claims description 105
- 101710144128 Non-structural protein 2 Proteins 0.000 claims description 105
- 101710199667 Nuclear export protein Proteins 0.000 claims description 105
- 241000711895 Bovine orthopneumovirus Species 0.000 claims description 91
- 238000000034 method Methods 0.000 claims description 76
- 230000036961 partial effect Effects 0.000 claims description 71
- 238000006073 displacement reaction Methods 0.000 claims description 69
- 238000006467 substitution reaction Methods 0.000 claims description 58
- 108700026244 Open Reading Frames Proteins 0.000 claims description 56
- 230000002163 immunogen Effects 0.000 claims description 49
- 241000283690 Bos taurus Species 0.000 claims description 48
- 210000002345 respiratory system Anatomy 0.000 claims description 48
- 101710158312 DNA-binding protein HU-beta Proteins 0.000 claims description 46
- 101710128560 Initiator protein NS1 Proteins 0.000 claims description 46
- 101710144127 Non-structural protein 1 Proteins 0.000 claims description 46
- 241000282414 Homo sapiens Species 0.000 claims description 45
- 230000012010 growth Effects 0.000 claims description 41
- 230000000890 antigenic effect Effects 0.000 claims description 40
- 230000003612 virological effect Effects 0.000 claims description 39
- 230000035897 transcription Effects 0.000 claims description 33
- 238000013518 transcription Methods 0.000 claims description 33
- 101150082239 G gene Proteins 0.000 claims description 32
- 101150107578 SH gene Proteins 0.000 claims description 32
- 239000002245 particle Substances 0.000 claims description 32
- 101150034814 F gene Proteins 0.000 claims description 30
- 244000052769 pathogen Species 0.000 claims description 27
- 108010046722 Thrombospondin 1 Proteins 0.000 claims description 25
- 230000001105 regulatory effect Effects 0.000 claims description 24
- 230000002829 reductive effect Effects 0.000 claims description 23
- 230000005847 immunogenicity Effects 0.000 claims description 21
- 230000002068 genetic effect Effects 0.000 claims description 20
- 108020004705 Codon Proteins 0.000 claims description 19
- 101000926057 Human herpesvirus 2 (strain G) Envelope glycoprotein C Proteins 0.000 claims description 19
- 102100036034 Thrombospondin-1 Human genes 0.000 claims description 19
- 239000012528 membrane Substances 0.000 claims description 19
- 241000282412 Homo Species 0.000 claims description 18
- 241000712079 Measles morbillivirus Species 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 18
- 108091000058 GTP-Binding Proteins 0.000 claims description 17
- 230000000295 complement effect Effects 0.000 claims description 17
- 108091006027 G proteins Proteins 0.000 claims description 16
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 16
- 239000012634 fragment Substances 0.000 claims description 16
- 101150046652 M2 gene Proteins 0.000 claims description 15
- 102000030782 GTP binding Human genes 0.000 claims description 14
- 101150095629 NS2 gene Proteins 0.000 claims description 14
- 108091092724 Noncoding DNA Proteins 0.000 claims description 14
- 108010068327 4-hydroxyphenylpyruvate dioxygenase Proteins 0.000 claims description 13
- 230000035945 sensitivity Effects 0.000 claims description 13
- 102000002508 Peptide Elongation Factors Human genes 0.000 claims description 12
- 108010068204 Peptide Elongation Factors Proteins 0.000 claims description 12
- 238000007792 addition Methods 0.000 claims description 12
- 230000001717 pathogenic effect Effects 0.000 claims description 12
- 102000004127 Cytokines Human genes 0.000 claims description 10
- 108090000695 Cytokines Proteins 0.000 claims description 10
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 10
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 10
- 101150062031 L gene Proteins 0.000 claims description 10
- 241000711798 Rabies lyssavirus Species 0.000 claims description 10
- 239000013604 expression vector Substances 0.000 claims description 10
- 230000009467 reduction Effects 0.000 claims description 10
- 101001039853 Sonchus yellow net virus Matrix protein Proteins 0.000 claims description 9
- 238000002679 ablation Methods 0.000 claims description 9
- 238000010348 incorporation Methods 0.000 claims description 9
- 108091029795 Intergenic region Proteins 0.000 claims description 8
- -1 M20RF2 Proteins 0.000 claims description 8
- 208000002606 Paramyxoviridae Infections Diseases 0.000 claims description 8
- 108010089430 Phosphoproteins Proteins 0.000 claims description 7
- 102000007982 Phosphoproteins Human genes 0.000 claims description 7
- 238000012259 partial gene deletion Methods 0.000 claims description 7
- 241000711950 Filoviridae Species 0.000 claims description 6
- 241000726041 Human respirovirus 1 Species 0.000 claims description 6
- 241000712003 Human respirovirus 3 Species 0.000 claims description 6
- 241001559187 Human rubulavirus 2 Species 0.000 claims description 6
- 230000006978 adaptation Effects 0.000 claims description 6
- 230000001086 cytosolic effect Effects 0.000 claims description 6
- 210000000987 immune system Anatomy 0.000 claims description 6
- 241000710929 Alphavirus Species 0.000 claims description 5
- 241000701022 Cytomegalovirus Species 0.000 claims description 5
- 241000710831 Flavivirus Species 0.000 claims description 5
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 5
- 241000701806 Human papillomavirus Species 0.000 claims description 5
- 101150033828 NS1 gene Proteins 0.000 claims description 5
- 241000700584 Simplexvirus Species 0.000 claims description 5
- 241000711386 Mumps virus Species 0.000 claims description 4
- 239000000443 aerosol Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000014621 translational initiation Effects 0.000 claims description 4
- 101710204837 Envelope small membrane protein Proteins 0.000 claims description 3
- 101150039660 HA gene Proteins 0.000 claims description 3
- 241000713772 Human immunodeficiency virus 1 Species 0.000 claims description 3
- 241000713340 Human immunodeficiency virus 2 Species 0.000 claims description 3
- 101710120037 Toxin CcdB Proteins 0.000 claims description 3
- 230000008774 maternal effect Effects 0.000 claims description 3
- 244000000010 microbial pathogen Species 0.000 claims description 3
- 230000004936 stimulating effect Effects 0.000 claims description 3
- 101000977023 Azospirillum brasilense Uncharacterized 17.8 kDa protein in nodG 5'region Proteins 0.000 claims description 2
- 101000961984 Bacillus thuringiensis Uncharacterized 30.3 kDa protein Proteins 0.000 claims description 2
- 101000644901 Drosophila melanogaster Putative 115 kDa protein in type-1 retrotransposable element R1DM Proteins 0.000 claims description 2
- 101000747702 Enterobacteria phage N4 Uncharacterized protein Gp2 Proteins 0.000 claims description 2
- 101000758599 Escherichia coli Uncharacterized 14.7 kDa protein Proteins 0.000 claims description 2
- 101710133291 Hemagglutinin-neuraminidase Proteins 0.000 claims description 2
- 101000768930 Lactococcus lactis subsp. cremoris Uncharacterized protein in pepC 5'region Proteins 0.000 claims description 2
- 101000976302 Leptospira interrogans Uncharacterized protein in sph 3'region Proteins 0.000 claims description 2
- 101000778886 Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) Uncharacterized protein LA_2151 Proteins 0.000 claims description 2
- 101001121571 Rice tungro bacilliform virus (isolate Philippines) Protein P2 Proteins 0.000 claims description 2
- 101000818098 Spirochaeta aurantia Uncharacterized protein in trpE 3'region Proteins 0.000 claims description 2
- 101001026590 Streptomyces cinnamonensis Putative polyketide beta-ketoacyl synthase 2 Proteins 0.000 claims description 2
- 101000750896 Synechococcus elongatus (strain PCC 7942 / FACHB-805) Uncharacterized protein Synpcc7942_2318 Proteins 0.000 claims description 2
- 101000916321 Xenopus laevis Transposon TX1 uncharacterized 149 kDa protein Proteins 0.000 claims description 2
- 101000760088 Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 / NCIMB 8938 / NRRL B-806 / ZM1) 20.9 kDa protein Proteins 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 239000003550 marker Substances 0.000 claims description 2
- 241000713112 Orthobunyavirus Species 0.000 claims 2
- 101150046453 NSL gene Proteins 0.000 claims 1
- 101710088839 Replication initiation protein Proteins 0.000 claims 1
- 206010022000 influenza Diseases 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 description 98
- 239000002299 complementary DNA Substances 0.000 description 63
- 230000010076 replication Effects 0.000 description 51
- 235000001014 amino acid Nutrition 0.000 description 47
- 210000004027 cell Anatomy 0.000 description 45
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 42
- 229940024606 amino acid Drugs 0.000 description 36
- 150000001413 amino acids Chemical class 0.000 description 36
- 241000724205 Rice stripe tenuivirus Species 0.000 description 33
- 108020004999 messenger RNA Proteins 0.000 description 27
- 241000711975 Vesicular stomatitis virus Species 0.000 description 23
- 230000008030 elimination Effects 0.000 description 22
- 238000003379 elimination reaction Methods 0.000 description 22
- 238000000338 in vitro Methods 0.000 description 20
- 206010061603 Respiratory syncytial virus infection Diseases 0.000 description 18
- 101150103632 M2-2 gene Proteins 0.000 description 16
- 241000282579 Pan Species 0.000 description 15
- 108010067390 Viral Proteins Proteins 0.000 description 15
- 230000001965 increasing effect Effects 0.000 description 15
- 238000004113 cell culture Methods 0.000 description 14
- 238000002703 mutagenesis Methods 0.000 description 14
- 231100000350 mutagenesis Toxicity 0.000 description 14
- 108091026890 Coding region Proteins 0.000 description 13
- 229940124679 RSV vaccine Drugs 0.000 description 13
- 239000003795 chemical substances by application Substances 0.000 description 12
- 201000010099 disease Diseases 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 238000001727 in vivo Methods 0.000 description 12
- 239000013612 plasmid Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 108020003175 receptors Proteins 0.000 description 12
- 108090001074 Nucleocapsid Proteins Proteins 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 11
- 230000036039 immunity Effects 0.000 description 11
- 238000001890 transfection Methods 0.000 description 11
- 238000011161 development Methods 0.000 description 10
- 230000018109 developmental process Effects 0.000 description 10
- 241001493065 dsRNA viruses Species 0.000 description 10
- 230000003053 immunization Effects 0.000 description 10
- 102000005962 receptors Human genes 0.000 description 10
- 238000002649 immunization Methods 0.000 description 9
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 description 8
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 8
- 238000010276 construction Methods 0.000 description 8
- 230000006870 function Effects 0.000 description 8
- 230000001976 improved effect Effects 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 230000003472 neutralizing effect Effects 0.000 description 8
- 208000024891 symptom Diseases 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 210000003501 vero cell Anatomy 0.000 description 8
- 230000001018 virulence Effects 0.000 description 8
- 101710141454 Nucleoprotein Proteins 0.000 description 7
- 241000700618 Vaccinia virus Species 0.000 description 7
- 239000002671 adjuvant Substances 0.000 description 7
- 230000004075 alteration Effects 0.000 description 7
- 230000008901 benefit Effects 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 238000002255 vaccination Methods 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 102000000588 Interleukin-2 Human genes 0.000 description 6
- 108010002350 Interleukin-2 Proteins 0.000 description 6
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 101710181008 P protein Proteins 0.000 description 6
- 101710177166 Phosphoprotein Proteins 0.000 description 6
- 108010008038 Synthetic Vaccines Proteins 0.000 description 6
- 101710137500 T7 RNA polymerase Proteins 0.000 description 6
- 229940124551 recombinant vaccine Drugs 0.000 description 6
- 238000003786 synthesis reaction Methods 0.000 description 6
- 238000013519 translation Methods 0.000 description 6
- 229940125575 vaccine candidate Drugs 0.000 description 6
- 108090000994 Catalytic RNA Proteins 0.000 description 5
- 102000053642 Catalytic RNA Human genes 0.000 description 5
- 108020004635 Complementary DNA Proteins 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 241001529936 Murinae Species 0.000 description 5
- 206010035664 Pneumonia Diseases 0.000 description 5
- 241000711902 Pneumovirus Species 0.000 description 5
- 241000283984 Rodentia Species 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 230000002209 hydrophobic effect Effects 0.000 description 5
- 230000000977 initiatory effect Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 108091092562 ribozyme Proteins 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 201000005505 Measles Diseases 0.000 description 4
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 4
- 208000005647 Mumps Diseases 0.000 description 4
- 241000711408 Murine respirovirus Species 0.000 description 4
- 241000282577 Pan troglodytes Species 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Chemical compound CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 4
- 230000004927 fusion Effects 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 238000010353 genetic engineering Methods 0.000 description 4
- 229940124590 live attenuated vaccine Drugs 0.000 description 4
- 229940023012 live-attenuated vaccine Drugs 0.000 description 4
- 230000001404 mediated effect Effects 0.000 description 4
- 230000000116 mitigating effect Effects 0.000 description 4
- 208000010805 mumps infectious disease Diseases 0.000 description 4
- 210000001989 nasopharynx Anatomy 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 206010039083 rhinitis Diseases 0.000 description 4
- 230000003248 secreting effect Effects 0.000 description 4
- 230000005029 transcription elongation Effects 0.000 description 4
- 230000029812 viral genome replication Effects 0.000 description 4
- 108010017213 Granulocyte-Macrophage Colony-Stimulating Factor Proteins 0.000 description 3
- 102000004457 Granulocyte-Macrophage Colony-Stimulating Factor Human genes 0.000 description 3
- 102000014150 Interferons Human genes 0.000 description 3
- 108010050904 Interferons Proteins 0.000 description 3
- 102000004388 Interleukin-4 Human genes 0.000 description 3
- 108090000978 Interleukin-4 Proteins 0.000 description 3
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 3
- 235000019687 Lamb Nutrition 0.000 description 3
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 3
- 208000035415 Reinfection Diseases 0.000 description 3
- 241000220317 Rosa Species 0.000 description 3
- 241000144282 Sigmodon Species 0.000 description 3
- 101710172711 Structural protein Proteins 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 239000013553 cell monolayer Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 239000012636 effector Substances 0.000 description 3
- 230000000763 evoking effect Effects 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 230000001506 immunosuppresive effect Effects 0.000 description 3
- 229940079322 interferon Drugs 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000000644 propagated effect Effects 0.000 description 3
- 238000011321 prophylaxis Methods 0.000 description 3
- 108020001580 protein domains Proteins 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 241000712461 unidentified influenza virus Species 0.000 description 3
- 210000002845 virion Anatomy 0.000 description 3
- DRHZYJAUECRAJM-DWSYSWFDSA-N (2s,3s,4s,5r,6r)-6-[[(3s,4s,4ar,6ar,6bs,8r,8ar,12as,14ar,14br)-8a-[(2s,3r,4s,5r,6r)-3-[(2s,3r,4s,5r,6s)-5-[(2s,3r,4s,5r)-4-[(2s,3r,4r)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]oxy-3,5-dihydroxyoxan-2-yl]oxy-3,4-dihydroxy-6-methyloxan-2-yl]oxy-5-[(3s,5s, Chemical compound O([C@H]1[C@H](O)[C@H](O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O1)O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@H]5CC(C)(C)CC[C@@]5([C@@H](C[C@@]4(C)[C@]3(C)CC[C@H]2[C@@]1(C=O)C)O)C(=O)O[C@@H]1O[C@H](C)[C@@H]([C@@H]([C@H]1O[C@H]1[C@@H]([C@H](O)[C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@](O)(CO)CO3)O)[C@H](O)CO2)O)[C@H](C)O1)O)O)OC(=O)C[C@@H](O)C[C@H](OC(=O)C[C@@H](O)C[C@@H]([C@@H](C)CC)O[C@H]1[C@@H]([C@@H](O)[C@H](CO)O1)O)[C@@H](C)CC)C(O)=O)[C@@H]1OC[C@@H](O)[C@H](O)[C@H]1O DRHZYJAUECRAJM-DWSYSWFDSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 2
- 208000031504 Asymptomatic Infections Diseases 0.000 description 2
- 241000711404 Avian avulavirus 1 Species 0.000 description 2
- 241000712083 Canine morbillivirus Species 0.000 description 2
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 2
- 108010079245 Cystic Fibrosis Transmembrane Conductance Regulator Proteins 0.000 description 2
- 102100023419 Cystic fibrosis transmembrane conductance regulator Human genes 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 101150008820 HN gene Proteins 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 2
- 102000008070 Interferon-gamma Human genes 0.000 description 2
- 108010074328 Interferon-gamma Proteins 0.000 description 2
- 102000013462 Interleukin-12 Human genes 0.000 description 2
- 108010065805 Interleukin-12 Proteins 0.000 description 2
- 102000003810 Interleukin-18 Human genes 0.000 description 2
- 108090000171 Interleukin-18 Proteins 0.000 description 2
- 101710145006 Lysis protein Proteins 0.000 description 2
- 101150039699 M2-1 gene Proteins 0.000 description 2
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 description 2
- 101710085938 Matrix protein Proteins 0.000 description 2
- 102000018697 Membrane Proteins Human genes 0.000 description 2
- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 101710127721 Membrane protein Proteins 0.000 description 2
- 241000711513 Mononegavirales Species 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 206010033078 Otitis media Diseases 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 208000018569 Respiratory Tract disease Diseases 0.000 description 2
- 241000711897 Rinderpest morbillivirus Species 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 108700005077 Viral Genes Proteins 0.000 description 2
- 108020000999 Viral RNA Proteins 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 229940031567 attenuated vaccine Drugs 0.000 description 2
- 230000037429 base substitution Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229940031416 bivalent vaccine Drugs 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 239000002962 chemical mutagen Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 210000005220 cytoplasmic tail Anatomy 0.000 description 2
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001747 exhibiting effect Effects 0.000 description 2
- 229940044627 gamma-interferon Drugs 0.000 description 2
- 238000012224 gene deletion Methods 0.000 description 2
- 230000013595 glycosylation Effects 0.000 description 2
- 238000006206 glycosylation reaction Methods 0.000 description 2
- 230000007236 host immunity Effects 0.000 description 2
- 229960002591 hydroxyproline Drugs 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000002134 immunopathologic effect Effects 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000001524 infective effect Effects 0.000 description 2
- 229940028885 interleukin-4 Drugs 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000004264 monolayer culture Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 208000023504 respiratory system disease Diseases 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 229940031626 subunit vaccine Drugs 0.000 description 2
- 230000000699 topical effect Effects 0.000 description 2
- 230000013715 transcription antitermination Effects 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- BEJKOYIMCGMNRB-GRHHLOCNSA-N (2s)-2-amino-3-(4-hydroxyphenyl)propanoic acid;(2s)-2-amino-3-phenylpropanoic acid Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1.OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 BEJKOYIMCGMNRB-GRHHLOCNSA-N 0.000 description 1
- ICLYJLBTOGPLMC-KVVVOXFISA-N (z)-octadec-9-enoate;tris(2-hydroxyethyl)azanium Chemical compound OCCN(CCO)CCO.CCCCCCCC\C=C/CCCCCCCC(O)=O ICLYJLBTOGPLMC-KVVVOXFISA-N 0.000 description 1
- HOURAEXAZWBEAW-UHFFFAOYSA-N 1-aminopropane-1,1,3-tricarboxylic acid Chemical compound OC(=O)C(C(O)=O)(N)CCC(O)=O HOURAEXAZWBEAW-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 1
- BRMWTNUJHUMWMS-UHFFFAOYSA-N 3-Methylhistidine Natural products CN1C=NC(CC(N)C(O)=O)=C1 BRMWTNUJHUMWMS-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- FHIDNBAQOFJWCA-UAKXSSHOSA-N 5-fluorouridine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(F)=C1 FHIDNBAQOFJWCA-UAKXSSHOSA-N 0.000 description 1
- 229940117976 5-hydroxylysine Drugs 0.000 description 1
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 241000272478 Aquila Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 241001519465 Avian metapneumovirus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000724653 Borna disease virus Species 0.000 description 1
- 241000776207 Bornaviridae Species 0.000 description 1
- 206010006448 Bronchiolitis Diseases 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 241000122205 Chamaeleonidae Species 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 201000006306 Cor pulmonale Diseases 0.000 description 1
- 241000759568 Corixa Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 150000008574 D-amino acids Chemical class 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241001115402 Ebolavirus Species 0.000 description 1
- 101000618318 Enterobacteria phage T4 Uncharacterized 8.2 kDa protein in Gp46-Gp47 intergenic region Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 101710160621 Fusion glycoprotein F0 Proteins 0.000 description 1
- 108010017544 Glucosylceramidase Proteins 0.000 description 1
- 102000004547 Glucosylceramidase Human genes 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- UYTPUPDQBNUYGX-UHFFFAOYSA-N Guanine Natural products O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 108090001102 Hammerhead ribozyme Proteins 0.000 description 1
- 101710189078 Helicase Proteins 0.000 description 1
- 208000005331 Hepatitis D Diseases 0.000 description 1
- 241000724709 Hepatitis delta virus Species 0.000 description 1
- FDGQSTZJBFJUBT-UHFFFAOYSA-N Hypoxanthine Natural products O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010062016 Immunosuppression Diseases 0.000 description 1
- 102000004889 Interleukin-6 Human genes 0.000 description 1
- 108090001005 Interleukin-6 Proteins 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001115401 Marburgvirus Species 0.000 description 1
- 241000712045 Morbillivirus Species 0.000 description 1
- 108010021466 Mutant Proteins Proteins 0.000 description 1
- 102000008300 Mutant Proteins Human genes 0.000 description 1
- MXNRLFUSFKVQSK-QMMMGPOBSA-O N(6),N(6),N(6)-trimethyl-L-lysine Chemical compound C[N+](C)(C)CCCC[C@H]([NH3+])C([O-])=O MXNRLFUSFKVQSK-QMMMGPOBSA-O 0.000 description 1
- JDHILDINMRGULE-LURJTMIESA-N N(pros)-methyl-L-histidine Chemical compound CN1C=NC=C1C[C@H](N)C(O)=O JDHILDINMRGULE-LURJTMIESA-N 0.000 description 1
- JJIHLJJYMXLCOY-BYPYZUCNSA-N N-acetyl-L-serine Chemical compound CC(=O)N[C@@H](CO)C(O)=O JJIHLJJYMXLCOY-BYPYZUCNSA-N 0.000 description 1
- PYUSHNKNPOHWEZ-YFKPBYRVSA-N N-formyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC=O PYUSHNKNPOHWEZ-YFKPBYRVSA-N 0.000 description 1
- 230000004988 N-glycosylation Effects 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 101150084044 P gene Proteins 0.000 description 1
- 102100034574 P protein Human genes 0.000 description 1
- 241000711504 Paramyxoviridae Species 0.000 description 1
- 241000711502 Paramyxovirinae Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 108010069013 Phenylalanine Hydroxylase Proteins 0.000 description 1
- 102100038223 Phenylalanine-4-hydroxylase Human genes 0.000 description 1
- 241000711904 Pneumoviridae Species 0.000 description 1
- 101710183921 Protein M2-1 Proteins 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 101710118046 RNA-directed RNA polymerase Proteins 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 241000711931 Rhabdoviridae Species 0.000 description 1
- 208000036071 Rhinorrhea Diseases 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 206010051497 Rhinotracheitis Diseases 0.000 description 1
- IWUCXVSUMQZMFG-AFCXAGJDSA-N Ribavirin Chemical compound N1=C(C(=O)N)N=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 IWUCXVSUMQZMFG-AFCXAGJDSA-N 0.000 description 1
- 102100030852 Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Human genes 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000251131 Sphyrna Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108091027544 Subgenomic mRNA Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000024932 T cell mediated immunity Effects 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 1
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 1
- 241000711955 Turkey rhinotracheitis virus Species 0.000 description 1
- 206010046865 Vaccinia virus infection Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241001428384 Zamora Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000002730 additional effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229940037003 alum Drugs 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- ILRRQNADMUWWFW-UHFFFAOYSA-K aluminium phosphate Chemical compound O1[Al]2OP1(=O)O2 ILRRQNADMUWWFW-UHFFFAOYSA-K 0.000 description 1
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 125000000613 asparagine group Chemical group N[C@@H](CC(N)=O)C(=O)* 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000036760 body temperature Effects 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000000981 bystander Effects 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 235000011148 calcium chloride Nutrition 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 230000002301 combined effect Effects 0.000 description 1
- 230000001447 compensatory effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 208000027744 congestion Diseases 0.000 description 1
- 108091036078 conserved sequence Proteins 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 231100000599 cytotoxic agent Toxicity 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 239000002619 cytotoxin Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 231100000517 death Toxicity 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- YSMODUONRAFBET-UHFFFAOYSA-N delta-DL-hydroxylysine Natural products NCC(O)CCC(N)C(O)=O YSMODUONRAFBET-UHFFFAOYSA-N 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- MWRBNPKJOOWZPW-CLFAGFIQSA-N dioleoyl phosphatidylethanolamine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(COP(O)(=O)OCCN)OC(=O)CCCCCCC\C=C/CCCCCCCC MWRBNPKJOOWZPW-CLFAGFIQSA-N 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- YSMODUONRAFBET-UHNVWZDZSA-N erythro-5-hydroxy-L-lysine Chemical compound NC[C@H](O)CC[C@H](N)C(O)=O YSMODUONRAFBET-UHNVWZDZSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960002949 fluorouracil Drugs 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 208000029570 hepatitis D virus infection Diseases 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 108700023453 human respiratory syncytial virus F Proteins 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 125000001841 imino group Chemical group [H]N=* 0.000 description 1
- 230000001759 immunoprophylactic effect Effects 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229940117681 interleukin-12 Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229940035032 monophosphoryl lipid a Drugs 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 231100000299 mutagenicity Toxicity 0.000 description 1
- 230000007886 mutagenicity Effects 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 239000006199 nebulizer Substances 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 210000001331 nose Anatomy 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- BZQFBWGGLXLEPQ-REOHCLBHSA-N phosphoserine Chemical compound OC(=O)[C@@H](N)COP(O)(O)=O BZQFBWGGLXLEPQ-REOHCLBHSA-N 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 238000011809 primate model Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 208000030925 respiratory syncytial virus infectious disease Diseases 0.000 description 1
- 208000020029 respiratory tract infectious disease Diseases 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 229960000329 ribavirin Drugs 0.000 description 1
- HZCAHMRRMINHDJ-DBRKOABJSA-N ribavirin Natural products O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1N=CN=C1 HZCAHMRRMINHDJ-DBRKOABJSA-N 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000015424 sodium Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000010473 stable expression Effects 0.000 description 1
- 239000008174 sterile solution Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 229940117013 triethanolamine oleate Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 208000007089 vaccinia Diseases 0.000 description 1
- 229940126580 vector vaccine Drugs 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- 230000009447 viral pathogenesis Effects 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/155—Paramyxoviridae, e.g. parainfluenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
- A61K2039/541—Mucosal route
- A61K2039/543—Mucosal route intranasal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18522—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18534—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18541—Use of virus, viral particle or viral elements as a vector
- C12N2760/18543—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
- C12N2760/18561—Methods of inactivation or attenuation
- C12N2760/18562—Methods of inactivation or attenuation by genetic engineering
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Biochemistry (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Pulmonology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21370800P | 2000-06-23 | 2000-06-23 | |
| PCT/US2001/020107 WO2002000693A2 (en) | 2000-06-23 | 2001-06-22 | Respiratory syncytial virus vaccines expressing protective antigens from promotor-proximal genes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA02012818A true MXPA02012818A (es) | 2003-05-14 |
Family
ID=22796187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA02012818A MXPA02012818A (es) | 2000-06-23 | 2001-06-22 | Vacunas de virus sincitales respiratoriso que expresan antigenos protectores a partir de genes proximos promotores. |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP1294858B1 (enExample) |
| JP (1) | JP2004515218A (enExample) |
| KR (1) | KR100899030B1 (enExample) |
| CN (1) | CN1468301A (enExample) |
| AT (1) | ATE416251T1 (enExample) |
| AU (3) | AU2001268709B2 (enExample) |
| BR (1) | BRPI0112276B8 (enExample) |
| CA (1) | CA2413786A1 (enExample) |
| DE (1) | DE60136811D1 (enExample) |
| ES (1) | ES2317914T3 (enExample) |
| IL (1) | IL153530A0 (enExample) |
| MX (1) | MXPA02012818A (enExample) |
| WO (1) | WO2002000693A2 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101260386A (zh) | 1999-07-09 | 2008-09-10 | 美国政府健康及人类服务部 | 减毒的人-牛嵌合呼吸道合胞病毒疫苗的生产 |
| EP2044198A4 (en) | 2006-07-27 | 2010-09-08 | Ligocyte Pharmaceuticals Inc | CHIMERIC INFLUENZA VIRUS TYPE PARTICLES |
| WO2008094200A2 (en) * | 2006-07-27 | 2008-08-07 | Ligocyte Pharmaceuticals, Inc | Chimeric virus-like particles |
| US8920812B2 (en) | 2009-11-03 | 2014-12-30 | Takeda Vaccines, Inc. | Chimeric RSV-F polypeptide and lentivirus or alpha-retrovirus Gag-based VLPS |
| CN105200069A (zh) * | 2015-10-16 | 2015-12-30 | 北京交通大学 | 基于rna聚合酶ⅰ的rsv微型复制基因组及其应用 |
| KR20180085802A (ko) | 2015-12-11 | 2018-07-27 | 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 | Ns1 및 ns2 유전자 이동을 포함하는 재조합 rsv 균주 |
| CN110229219B (zh) * | 2019-06-21 | 2021-03-30 | 中国科学院武汉病毒研究所 | 一种新型的呼吸道合胞病毒疫苗抗原的制备方法及其用途 |
| US20230279362A1 (en) * | 2020-06-05 | 2023-09-07 | The United States Of America, As Represented By The Secretary, Department Of Health And Human | Live attenuated respiratory syncytial virus |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0912724B1 (en) * | 1996-07-15 | 2009-12-09 | The Government of The United States of America, as represented by The Department of Health and Human Services | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences |
-
2001
- 2001-06-22 BR BRPI0112276-2 patent/BRPI0112276B8/pt not_active IP Right Cessation
- 2001-06-22 AU AU2001268709A patent/AU2001268709B2/en not_active Ceased
- 2001-06-22 MX MXPA02012818A patent/MXPA02012818A/es active IP Right Grant
- 2001-06-22 AT AT01946696T patent/ATE416251T1/de not_active IP Right Cessation
- 2001-06-22 DE DE60136811T patent/DE60136811D1/de not_active Expired - Lifetime
- 2001-06-22 IL IL15353001A patent/IL153530A0/xx not_active IP Right Cessation
- 2001-06-22 CN CNA018143628A patent/CN1468301A/zh active Pending
- 2001-06-22 KR KR1020027017577A patent/KR100899030B1/ko not_active Expired - Fee Related
- 2001-06-22 AU AU6870901A patent/AU6870901A/xx active Pending
- 2001-06-22 WO PCT/US2001/020107 patent/WO2002000693A2/en not_active Ceased
- 2001-06-22 JP JP2002505815A patent/JP2004515218A/ja active Pending
- 2001-06-22 EP EP01946696A patent/EP1294858B1/en not_active Expired - Lifetime
- 2001-06-22 CA CA002413786A patent/CA2413786A1/en not_active Abandoned
- 2001-06-22 ES ES01946696T patent/ES2317914T3/es not_active Expired - Lifetime
-
2006
- 2006-12-22 AU AU2006252238A patent/AU2006252238A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ES2317914T3 (es) | 2009-05-01 |
| AU2001268709B2 (en) | 2006-09-28 |
| BR0112276A (pt) | 2004-02-10 |
| IL153530A0 (en) | 2003-07-06 |
| BRPI0112276B1 (pt) | 2019-12-31 |
| KR100899030B1 (ko) | 2009-05-21 |
| CN1468301A (zh) | 2004-01-14 |
| BRPI0112276B8 (pt) | 2021-05-25 |
| ATE416251T1 (de) | 2008-12-15 |
| WO2002000693A3 (en) | 2002-09-26 |
| EP1294858A2 (en) | 2003-03-26 |
| JP2004515218A (ja) | 2004-05-27 |
| EP1294858B1 (en) | 2008-12-03 |
| KR20030045685A (ko) | 2003-06-11 |
| CA2413786A1 (en) | 2002-01-03 |
| AU2006252238A1 (en) | 2007-01-25 |
| AU6870901A (en) | 2002-01-08 |
| DE60136811D1 (de) | 2009-01-15 |
| WO2002000693A2 (en) | 2002-01-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100905450B1 (ko) | 약독화된 인간-소 키메릭 호흡기 신시티움 바이러스백신의 제조 방법 | |
| EP0912724B1 (en) | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences | |
| US7846455B2 (en) | Attenuated chimeric respiratory syncytial virus | |
| US6923971B2 (en) | Respiratory syncytial virus vaccines expressing protective antigens from promoter-proximal genes | |
| EP1690940A1 (en) | Production of attenuated chimeric respiratory syncytial virus vaccines from cloned nucleotide sequences | |
| US7744902B2 (en) | Respiratory syncytial virus vaccines expressing protective antigens from promotor-proximal genes | |
| EP1294858B1 (en) | Respiratory syncytial virus vaccines expressing protective antigens from promotor-proximal genes | |
| US7662397B2 (en) | Respiratory syncytial virus vaccines expressing protective antigens from promoter-proximal genes | |
| AU2001268709A1 (en) | Respiratory syncytial virus vaccines expressing protective antigens from promotor-proximal genes | |
| US7820182B2 (en) | Production of attenuated, human-bovine chimeric respiratory syncytial viruses for use in immunogenic compositions | |
| AU2008203034A1 (en) | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |