MX9702889A - Mejoramiento de un vector de expresion para la produccion de proteinas recombinantes. - Google Patents

Mejoramiento de un vector de expresion para la produccion de proteinas recombinantes.

Info

Publication number
MX9702889A
MX9702889A MX9702889A MX9702889A MX9702889A MX 9702889 A MX9702889 A MX 9702889A MX 9702889 A MX9702889 A MX 9702889A MX 9702889 A MX9702889 A MX 9702889A MX 9702889 A MX9702889 A MX 9702889A
Authority
MX
Mexico
Prior art keywords
expression vector
dna construct
gene
improvement
production
Prior art date
Application number
MX9702889A
Other languages
English (en)
Other versions
MXPA97002889A (es
Inventor
Gunnar Norstedt
Tim Wood
Daniel Sliva
Vertil Enberg
Peter Lobie
Lars-Arne Haldosen
Original Assignee
Pharmacia & Upjohn Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia & Upjohn Ab filed Critical Pharmacia & Upjohn Ab
Publication of MX9702889A publication Critical patent/MX9702889A/es
Publication of MXPA97002889A publication Critical patent/MXPA97002889A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/30—Vector systems having a special element relevant for transcription being an enhancer not forming part of the promoter region
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

Abstract

La invencion se refiere a un método para mejorar la transcripcion de un gen en una construccion de ADN incorporada en el genoma de una célula huésped eucariotica, cuya construccion de ADN comprende un gen estructural para una proteína o polipéptido deseado y un gen promotor aguas arriba del gen estructural. La invencion reside en proporcionar al menos un elemento mejorador que comprende la secuencia de nucleotidos TTC TGA GAA aguas arriba de dicho promotor y que expone la construccion de ADN al estímulo lactogénico. La invencion también se refiere a un vector de expresion y a una célula huésped y a un mamífero transgénico que contiene este vector.
MXPA/A/1997/002889A 1994-10-21 1997-04-21 Mejoramiento de un vector de expresion para la produccion de proteinas recombinantes MXPA97002889A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9403613A SE9403613D0 (sv) 1994-10-21 1994-10-21 Improvement of an expression vector for production of recombinant proteins
SE9403613-4 1994-10-21
PCT/SE1995/001235 WO1996012815A1 (en) 1994-10-21 1995-10-19 Improvement of an expression vector for production of recombinant proteins

Publications (2)

Publication Number Publication Date
MX9702889A true MX9702889A (es) 1997-07-31
MXPA97002889A MXPA97002889A (es) 1997-12-01

Family

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Also Published As

Publication number Publication date
PT782628E (pt) 2005-04-29
SE9403613D0 (sv) 1994-10-21
ATE284447T1 (de) 2004-12-15
DE69533840D1 (de) 2005-01-13
EP0782628A1 (en) 1997-07-09
DE69533840T2 (de) 2005-12-08
AU3820295A (en) 1996-05-15
US6773919B2 (en) 2004-08-10
EP1338653A3 (en) 2004-04-21
EP1338653A2 (en) 2003-08-27
WO1996012815A1 (en) 1996-05-02
AU705594B2 (en) 1999-05-27
NZ294968A (en) 1998-08-26
ES2235175T3 (es) 2005-07-01
US20030192064A1 (en) 2003-10-09
CA2200465A1 (en) 1996-05-02
JPH10507633A (ja) 1998-07-28
EP0782628B1 (en) 2004-12-08

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