MX9306174A - AFFINITY PURIFYING METHODS INVOLVING AMINO ACID MIMETICS AS A REVIVAL OF LEVIGATION. - Google Patents

AFFINITY PURIFYING METHODS INVOLVING AMINO ACID MIMETICS AS A REVIVAL OF LEVIGATION.

Info

Publication number
MX9306174A
MX9306174A MX9306174A MX9306174A MX9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A
Authority
MX
Mexico
Prior art keywords
complex
protein
molecule
amino acid
levigation
Prior art date
Application number
MX9306174A
Other languages
English (en)
Inventor
Brian Seed
Original Assignee
Gen Hospital Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US95666092A priority Critical
Application filed by Gen Hospital Corp filed Critical Gen Hospital Corp
Publication of MX9306174A publication Critical patent/MX9306174A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation

Abstract

Se describe un método para aislar una proteína de una muestra, incluyendo(i) proporcionar una primera molécula que es capaz de formar un complejode afinidad con la proteína; (ii) poner en contacto la muestra con laprimera molécula bajo condiciones que permitan formación de complejo deafinidad; (iii) aislar el complejo; (iv) tratar el complejo con unasegunda molécula, la segunda molécula imitando un residuo de aminoácido dela proteína o la primer molécula que es crítico para la formación decomplejo, de modo que la segunda molécula rompa el complejo, ocasionandola liberación de la proteína del complejo; y (v) aislar la proteína. Deacuerdo con una forma de realización, se usa amidazola mimética deaminoácido como un reactivo de levigación muy suave para romper elcomplejo de proteína de anticuerpo A- y proteína de fusión, una técnicaque tiene aplicación general para el aislamiento de anticuerpos oproteínas de fusión de anticuerpo, recombinantes.A method of isolating a protein from a sample is described, including (i) providing a first molecule that is capable of forming an affinity complex with the protein; (ii) contacting the sample with the first molecule under conditions that allow affinity complex formation; (iii) isolate the complex; (iv) treating the complex with a second molecule, the second molecule mimicking an amino acid residue of the protein, or the first molecule that is critical for complex formation, so that the second molecule breaks the complex, causing release of the protein from the complex; and (v) isolating the protein. According to one embodiment, amino acid mimetic amidazole is used as a very mild levigation reagent to break down the A-antibody protein and fusion protein complex, a technique that has general application for the isolation of antibodies, recombinant antibody fusion proteins .
MX9306174A 1992-10-02 1993-10-04 AFFINITY PURIFYING METHODS INVOLVING AMINO ACID MIMETICS AS A REVIVAL OF LEVIGATION. MX9306174A (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US95666092A true 1992-10-02 1992-10-02

Publications (1)

Publication Number Publication Date
MX9306174A true MX9306174A (es) 1995-01-31

Family

ID=25498506

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9306174A MX9306174A (es) 1992-10-02 1993-10-04 AFFINITY PURIFYING METHODS INVOLVING AMINO ACID MIMETICS AS A REVIVAL OF LEVIGATION.

Country Status (6)

Country Link
EP (1) EP0663925A4 (es)
JP (1) JPH08503698A (es)
AU (1) AU5292693A (es)
CA (1) CA2146162A1 (es)
MX (1) MX9306174A (es)
WO (1) WO1994007912A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1864999T1 (sl) 1996-11-27 2009-08-31 Genentech Inc Afinitetno čiščenje polipeptida na proteinskem A matriksu
AU4292801A (en) * 2000-03-16 2001-09-24 Biacore Ab Method for capturing analytes eluted from surface-bound ligands
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
CN102893146A (zh) 2010-05-19 2013-01-23 弗·哈夫曼-拉罗切有限公司 疏水相互作用色谱法
JP6236948B2 (ja) * 2013-07-17 2017-11-29 東ソー株式会社 抗体精製用溶出液および当該溶出液を用いた抗体精製方法
HRP20160086A2 (hr) 2016-01-27 2017-08-11 Sveučilište u Zagrebu Eluens za imunoafinitetnu kromatografiju virusa i proteina

Also Published As

Publication number Publication date
EP0663925A1 (en) 1995-07-26
CA2146162A1 (en) 1994-04-14
JPH08503698A (ja) 1996-04-23
WO1994007912A1 (en) 1994-04-14
EP0663925A4 (en) 1997-11-19
AU5292693A (en) 1994-04-26

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