MX9306174A - Metodos de purifiacion por afinidad que involucran mimeticos de aminoacidocomo reactivo de levigacion. - Google Patents
Metodos de purifiacion por afinidad que involucran mimeticos de aminoacidocomo reactivo de levigacion.Info
- Publication number
- MX9306174A MX9306174A MX9306174A MX9306174A MX9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A MX 9306174 A MX9306174 A MX 9306174A
- Authority
- MX
- Mexico
- Prior art keywords
- complex
- protein
- molecule
- amino acid
- levigation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
Se describe un método para aislar una proteína de una muestra, incluyendo(i) proporcionar una primera molécula que es capaz de formar un complejode afinidad con la proteína; (ii) poner en contacto la muestra con laprimera molécula bajo condiciones que permitan formación de complejo deafinidad; (iii) aislar el complejo; (iv) tratar el complejo con unasegunda molécula, la segunda molécula imitando un residuo de aminoácido dela proteína o la primer molécula que es crítico para la formación decomplejo, de modo que la segunda molécula rompa el complejo, ocasionandola liberación de la proteína del complejo; y (v) aislar la proteína. Deacuerdo con una forma de realización, se usa amidazola mimética deaminoácido como un reactivo de levigación muy suave para romper elcomplejo de proteína de anticuerpo A- y proteína de fusión, una técnicaque tiene aplicación general para el aislamiento de anticuerpos oproteínas de fusión de anticuerpo, recombinantes.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US95666092A | 1992-10-02 | 1992-10-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX9306174A true MX9306174A (es) | 1995-01-31 |
Family
ID=25498506
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX9306174A MX9306174A (es) | 1992-10-02 | 1993-10-04 | Metodos de purifiacion por afinidad que involucran mimeticos de aminoacidocomo reactivo de levigacion. |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0663925A4 (es) |
| JP (1) | JPH08503698A (es) |
| AU (1) | AU5292693A (es) |
| CA (1) | CA2146162A1 (es) |
| MX (1) | MX9306174A (es) |
| WO (1) | WO1994007912A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT950067E (pt) * | 1996-11-27 | 2007-12-06 | Genentech Inc | Purificação por afinidade de um polipéptido numa matriz de proteína a. |
| JP4860085B2 (ja) | 2000-03-16 | 2012-01-25 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 表面結合リガンドから溶出された分析物を捕捉するための方法 |
| US8084032B2 (en) | 2004-01-21 | 2011-12-27 | Ajinomoto Co., Inc. | Purification method which prevents denaturation of an antibody |
| JP5635682B2 (ja) | 2010-05-19 | 2014-12-03 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 疎水性相互作用クロマトグラフィー法 |
| JP6236948B2 (ja) * | 2013-07-17 | 2017-11-29 | 東ソー株式会社 | 抗体精製用溶出液および当該溶出液を用いた抗体精製方法 |
| HRP20160086A2 (hr) | 2016-01-27 | 2017-08-11 | Sveučilište u Zagrebu | Eluens za imunoafinitetnu kromatografiju virusa i proteina |
-
1993
- 1993-09-27 CA CA 2146162 patent/CA2146162A1/en not_active Abandoned
- 1993-09-27 AU AU52926/93A patent/AU5292693A/en not_active Abandoned
- 1993-09-27 WO PCT/US1993/009174 patent/WO1994007912A1/en not_active Application Discontinuation
- 1993-09-27 JP JP6509231A patent/JPH08503698A/ja active Pending
- 1993-09-27 EP EP93923139A patent/EP0663925A4/en not_active Withdrawn
- 1993-10-04 MX MX9306174A patent/MX9306174A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH08503698A (ja) | 1996-04-23 |
| AU5292693A (en) | 1994-04-26 |
| CA2146162A1 (en) | 1994-04-14 |
| WO1994007912A1 (en) | 1994-04-14 |
| EP0663925A4 (en) | 1997-11-19 |
| EP0663925A1 (en) | 1995-07-26 |
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