CA2146162A1 - Affinity purification methods involving amino acid mimetics as elution reagents - Google Patents

Affinity purification methods involving amino acid mimetics as elution reagents

Info

Publication number
CA2146162A1
CA2146162A1 CA 2146162 CA2146162A CA2146162A1 CA 2146162 A1 CA2146162 A1 CA 2146162A1 CA 2146162 CA2146162 CA 2146162 CA 2146162 A CA2146162 A CA 2146162A CA 2146162 A1 CA2146162 A1 CA 2146162A1
Authority
CA
Canada
Prior art keywords
protein
molecule
complex
amino acid
elution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2146162
Other languages
English (en)
French (fr)
Inventor
Brian Seed
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2146162A1 publication Critical patent/CA2146162A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CA 2146162 1992-10-02 1993-09-27 Affinity purification methods involving amino acid mimetics as elution reagents Abandoned CA2146162A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95666092A 1992-10-02 1992-10-02
US07/956,660 1992-10-02

Publications (1)

Publication Number Publication Date
CA2146162A1 true CA2146162A1 (en) 1994-04-14

Family

ID=25498506

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2146162 Abandoned CA2146162A1 (en) 1992-10-02 1993-09-27 Affinity purification methods involving amino acid mimetics as elution reagents

Country Status (6)

Country Link
EP (1) EP0663925A4 (es)
JP (1) JPH08503698A (es)
AU (1) AU5292693A (es)
CA (1) CA2146162A1 (es)
MX (1) MX9306174A (es)
WO (1) WO1994007912A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE425990T1 (de) * 1996-11-27 2009-04-15 Genentech Inc Affinitatsreinigung von polypeptid-proteinen auf einer matrix
WO2001069258A1 (en) 2000-03-16 2001-09-20 Biacore Ab Method for capturing analytes eluted from surface-bound ligands
US8084032B2 (en) 2004-01-21 2011-12-27 Ajinomoto Co., Inc. Purification method which prevents denaturation of an antibody
BR112012027262A2 (pt) 2010-05-19 2016-07-26 Hoffmann La Roche método para purificar e para produzir um polipeptídeo
JP6236948B2 (ja) * 2013-07-17 2017-11-29 東ソー株式会社 抗体精製用溶出液および当該溶出液を用いた抗体精製方法
HRP20160086A2 (hr) 2016-01-27 2017-08-11 Sveučilište u Zagrebu Eluens za imunoafinitetnu kromatografiju virusa i proteina

Also Published As

Publication number Publication date
JPH08503698A (ja) 1996-04-23
MX9306174A (es) 1995-01-31
WO1994007912A1 (en) 1994-04-14
EP0663925A1 (en) 1995-07-26
EP0663925A4 (en) 1997-11-19
AU5292693A (en) 1994-04-26

Similar Documents

Publication Publication Date Title
JP4031049B2 (ja) タンパク質精製
AU2002255678B2 (en) Cell separation compostions and methods
US6399750B1 (en) IGG separation medium
De Meester et al. Use of immobilized adenosine deaminase (EC 3.5. 4.4) for the rapid purification of native human CD26/dipeptidyl peptidase IV (EC 3.4. 14.5)
US4933435A (en) Antibody purification process
US5719269A (en) Chromatography adsorbents utilizing mercapto heterocyclic ligands
Engers et al. The fate of anti-Ig-surface Ig complexes on B lymphocytes
US5726293A (en) Affinity purification methods involving imidazole elution
CA2469815A1 (en) Methods for purifying protein
AU2002255678A1 (en) Cell separation compostions and methods
JPS63258500A (ja) 改良された免疫グロブリン結合蛋白質
CA2146162A1 (en) Affinity purification methods involving amino acid mimetics as elution reagents
EP3543251A1 (en) Solid support containing igg-binding peptide, and method for separating igg
Fujita-Yamaguchi Affinity chromatography of native and recombinant proteins from receptors for insulin and IGF-I to recombinant single chain antibodies
Ray et al. A biologically active receptor for the carbohydrate-binding protein (s) of Dictyostelium discoideum
Gafencu et al. Protein and fatty acid composition of caveolae from apical plasmalemma of aortic endothelial cells
Tomonaga et al. Effect of membrane fluidizers on the number and affinity of chemotactic factor receptors on human polymorphonuclear leukocytes
Ngo Rapid purification of immunoglobulin G using aza-arenophilic chromatography: novel mode of protein—solid phase interactions
JPH0696598B2 (ja) 抗体の精製法
Griffin et al. A Human Lymphokine Activates Macrophage C3 Receptors for Phagocytosis: Studies Using Monoclonal Anti‐Lymphokine Antibodies
JPH09504532A (ja) クロマトグラフィー法による第8因子を含むウィルス不活性化分画の製造方法
JPH0459797A (ja) 抗体の精製法
Rikihisa et al. Binding of markers of either side of plasma membranes to the cytoplasmic side of lysosomes
Woehler et al. Purification of DNP specific antibodies using sepharose bound DNP-p-aminobenzoylglutamate
Choi et al. A novel method for radiolabeling antigen-binding receptors of lymphocytes

Legal Events

Date Code Title Description
FZDE Dead