MX9302822A - Mejoras en un procedimiento para la produccion de una celula hospedante procariotica transformada. - Google Patents
Mejoras en un procedimiento para la produccion de una celula hospedante procariotica transformada.Info
- Publication number
- MX9302822A MX9302822A MX9302822A MX9302822A MX9302822A MX 9302822 A MX9302822 A MX 9302822A MX 9302822 A MX9302822 A MX 9302822A MX 9302822 A MX9302822 A MX 9302822A MX 9302822 A MX9302822 A MX 9302822A
- Authority
- MX
- Mexico
- Prior art keywords
- hospedant
- procariotic
- transformed
- procedure
- cell
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
El invento se refiere a mejoras en un procedimiento sencillo para la amplificación genética cromosomal estable de secuencias de ADN, que codifican un polipéptido en microorganismos procarióticos, especialmente en capas de Bacillus.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4216246A DE4216246A1 (de) | 1992-05-16 | 1992-05-16 | Einfaches Verfahren zur stabilen chromosomalen Genamplifikation |
Publications (1)
Publication Number | Publication Date |
---|---|
MX9302822A true MX9302822A (es) | 1993-11-01 |
Family
ID=6459063
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX9302822A MX9302822A (es) | 1992-05-16 | 1993-05-14 | Mejoras en un procedimiento para la produccion de una celula hospedante procariotica transformada. |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP0570787A3 (es) |
JP (1) | JPH06303986A (es) |
KR (1) | KR930023461A (es) |
BR (1) | BR9301900A (es) |
CA (1) | CA2096284A1 (es) |
DE (1) | DE4216246A1 (es) |
FI (1) | FI932215A (es) |
MX (1) | MX9302822A (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1261918A (zh) * | 1997-07-03 | 2000-08-02 | 诺沃挪第克公司 | 具有多拷贝目的基因和可筛选标记而不具选择性标记的产蛋白质细胞 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0134048B2 (en) * | 1983-07-06 | 1996-08-14 | Gist-Brocades N.V. | Molecular cloning and expression in industrial microorganism species |
FR2563533B1 (fr) * | 1984-04-27 | 1986-08-22 | Centre Nat Rech Scient | Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues |
FR2569723B1 (fr) * | 1984-08-31 | 1986-09-19 | Centre Nat Rech Scient | Application des antibiotiques de la famille des phleomycines a titre d'agent de selection dans le domaine du genie genetique |
AU620326B2 (en) * | 1987-02-27 | 1992-02-20 | Dsm Ip Assets B.V. | Stable gene amplification in chromosomal dna of prokaryotic microorganisms |
-
1992
- 1992-05-16 DE DE4216246A patent/DE4216246A1/de not_active Withdrawn
-
1993
- 1993-05-06 KR KR1019930007771A patent/KR930023461A/ko not_active Application Discontinuation
- 1993-05-08 EP EP9393107517A patent/EP0570787A3/de not_active Withdrawn
- 1993-05-14 FI FI932215A patent/FI932215A/fi unknown
- 1993-05-14 CA CA002096284A patent/CA2096284A1/en not_active Abandoned
- 1993-05-14 BR BR9301900A patent/BR9301900A/pt not_active Application Discontinuation
- 1993-05-14 MX MX9302822A patent/MX9302822A/es unknown
- 1993-05-14 JP JP5112830A patent/JPH06303986A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
DE4216246A1 (de) | 1993-11-18 |
CA2096284A1 (en) | 1993-11-17 |
FI932215A0 (fi) | 1993-05-14 |
KR930023461A (ko) | 1993-12-18 |
BR9301900A (pt) | 1993-11-23 |
EP0570787A2 (de) | 1993-11-24 |
JPH06303986A (ja) | 1994-11-01 |
EP0570787A3 (en) | 1994-08-17 |
FI932215A (fi) | 1993-11-17 |
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