MX394014B - Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas. - Google Patents

Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas.

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Publication number
MX394014B
MX394014B MX2018012554A MX2018012554A MX394014B MX 394014 B MX394014 B MX 394014B MX 2018012554 A MX2018012554 A MX 2018012554A MX 2018012554 A MX2018012554 A MX 2018012554A MX 394014 B MX394014 B MX 394014B
Authority
MX
Mexico
Prior art keywords
transgenic plants
haplotypes associated
improved stacked
trait performance
stacked trait
Prior art date
Application number
MX2018012554A
Other languages
English (en)
Other versions
MX2018012554A (es
Inventor
Curtis W Scherder
Dean A Ulbrich
Floyd G Hancock
Ivan Husic
James Narvel
Jesse J Gilsinger
Gelder Mindy L Devries
Paul Feng
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of MX2018012554A publication Critical patent/MX2018012554A/es
Publication of MX394014B publication Critical patent/MX394014B/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275Glyphosate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/172Haplotypes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a un método para identificar una planta de soya transgérnica que comprende un genotipo asociado con la tolerancia reproducida al glifosato, el método se caracteriza porque comprende: (a) marcar por lo menos una planta transgénica en una población de plantas de soya transgénica que se han expuesto a dicamba para la tolerancia a dicamba, dichas plantas tiene un transgen que confiere resistencia a dicamba; (b) seleccionar una panta transgénica que exhiba tolerancia a dicamba, y ( c) genotipar la planta de soya seleccionada con respecto a por lo menos un marcado de ácido nucleíco polimórfico en cada una de por lo menos dos regiones genómicas del grupo de enlace L seleccionadas de: i) una primera región genómica del grupo de enlace L flanqueada por los loci M0205928 (SEQ ID NO: 4) y M0129138 (SEQ ID NO: 6), en donde la región comprende el locus de M0101724 (SEQ ID NO:5); (ii) una segunda región genómica del grupo de enlace L que está flanqueada por los loci BU551363 (SEQ ID NO: 9) y BU765955 (SEQ ID NO: 12) , en donde la región comprende los loci MO205350 (SEQ ID NO: 10) Y MO102027 (SEQ ID NO:11), (iii) una tercer región genómica del grupo de enlace L que está flanqueada por los loci BU55345 (SEQ ID NO:7) y M0114388 (SEQ ID O: 8), en donde la región comprende el locus NGMAX008197032 (SEQ ID NO: 52); identificando de tal modo una planta de soya transgénica que comprende un genotipo asociado con la tolerancia reproductiva a glifosato.
MX2018012554A 2012-05-23 2013-05-23 Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas. MX394014B (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201261650869P 2012-05-23 2012-05-23
US201261650852P 2012-05-23 2012-05-23
US201361753693P 2013-01-17 2013-01-17
US201361753725P 2013-01-17 2013-01-17
US201361779739P 2013-03-13 2013-03-13
PCT/US2013/042349 WO2013177356A2 (en) 2012-05-23 2013-05-23 Haplotypes associated with improved stacked trait performance in transgenic plants

Publications (2)

Publication Number Publication Date
MX2018012554A MX2018012554A (es) 2022-07-18
MX394014B true MX394014B (es) 2025-03-24

Family

ID=49624520

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014014272A MX380383B (es) 2012-05-23 2013-05-23 Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas.
MX2018012554A MX394014B (es) 2012-05-23 2013-05-23 Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014014272A MX380383B (es) 2012-05-23 2013-05-23 Haplotipos asociados con el rendimiento del rasgo apilado mejorado en plantas transgenicas.

Country Status (7)

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US (2) US10604767B2 (es)
AR (1) AR091141A1 (es)
BR (1) BR112014028729B1 (es)
CA (1) CA2874470C (es)
MX (2) MX380383B (es)
WO (1) WO2013177356A2 (es)
ZA (1) ZA201408587B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2874470C (en) * 2012-05-23 2022-04-19 Monsanto Technology Llc Haplotypes associated with improved dicamba tolerance and glyphosate tolerance in transgenic soybean plants

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* Cited by examiner, † Cited by third party
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Also Published As

Publication number Publication date
BR112014028729A2 (es) 2017-10-03
US20200263192A1 (en) 2020-08-20
MX380383B (es) 2025-03-11
US20150143577A1 (en) 2015-05-21
AR091141A1 (es) 2015-01-14
MX2014014272A (es) 2015-04-08
US11661608B2 (en) 2023-05-30
ZA201408587B (en) 2022-12-21
WO2013177356A2 (en) 2013-11-28
CA2874470C (en) 2022-04-19
BR112014028729B1 (pt) 2022-05-17
CA2874470A1 (en) 2013-11-28
MX2018012554A (es) 2022-07-18
WO2013177356A3 (en) 2014-02-13
US10604767B2 (en) 2020-03-31

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