MX391007B - Concentracion, recuperacion y deteccion rapida de patogenos en muestras de alimentos. - Google Patents

Concentracion, recuperacion y deteccion rapida de patogenos en muestras de alimentos.

Info

Publication number
MX391007B
MX391007B MX2018000840A MX2018000840A MX391007B MX 391007 B MX391007 B MX 391007B MX 2018000840 A MX2018000840 A MX 2018000840A MX 2018000840 A MX2018000840 A MX 2018000840A MX 391007 B MX391007 B MX 391007B
Authority
MX
Mexico
Prior art keywords
detection
pathogens
recovery
food samples
rapid concentration
Prior art date
Application number
MX2018000840A
Other languages
English (en)
Other versions
MX2018000840A (es
Inventor
Alisha Tungare
Amanda Deering
Aquino Ximenes Eduardo De
James Thomas Jones
Jaycey Hardenstein
Kirk Solon Foster
Michael Ralph Ladisch
Seockmo Ku
Kreke Thomas Richard
Xingya LIU ( Linda)
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Publication of MX2018000840A publication Critical patent/MX2018000840A/es
Publication of MX391007B publication Critical patent/MX391007B/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2866—Grinding or homogeneising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/255—Salmonella (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a métodos para concentrar rápidamente una muestra alimenticia para detección eficiente de bacterias. Se usa un procedimiento de microfiltración seguido por centrifugación para concentrar las células con una enzima (por ejemplo, una proteasa) agregada en el inicio del proceso para facilitar micro-filtración más eficiente. Se encuentra la enzima para no tener efecto significativo en viabilidad celular.
MX2018000840A 2015-07-23 2016-07-22 Concentracion, recuperacion y deteccion rapida de patogenos en muestras de alimentos. MX391007B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562196216P 2015-07-23 2015-07-23
PCT/US2016/043596 WO2017015574A1 (en) 2015-07-23 2016-07-22 Rapid concentration, recovery and detection of pathogens in food samples

Publications (2)

Publication Number Publication Date
MX2018000840A MX2018000840A (es) 2018-05-04
MX391007B true MX391007B (es) 2025-03-21

Family

ID=57834722

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018000840A MX391007B (es) 2015-07-23 2016-07-22 Concentracion, recuperacion y deteccion rapida de patogenos en muestras de alimentos.

Country Status (5)

Country Link
US (1) US11789018B2 (es)
CN (1) CN107923825A (es)
CA (1) CA2991445C (es)
MX (1) MX391007B (es)
WO (1) WO2017015574A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11517900B2 (en) * 2017-10-27 2022-12-06 University Of Utah Research Foundation Microfluidic system for sperm separation and enrichment from various types of sperm samples
SG10201801064RA (en) * 2018-02-07 2019-09-27 Singapore Polytechnic System and method for on-site detection of pathogens
CA3111657A1 (en) 2018-09-05 2020-03-12 Pathotrak, Llc Rapid separation and recovery of pathogens from food samples by microfiltration assisted counterflow elutriation (mace)
FR3088719B1 (fr) 2018-11-16 2023-03-31 Commissariat Energie Atomique Procede de preparation d'un echantillon a analyser obtenu a partir de matrice alimentaire
EP3953678B1 (en) 2019-04-12 2024-08-28 Pathotrak, LLC Apparatuses and methods for rapid collection of microbe from a sample
CN111304301A (zh) * 2020-02-24 2020-06-19 欧陆分析技术服务(苏州)有限公司 一种bax pcr方法在食品沙门氏菌检测中的应用
GB202204947D0 (en) * 2022-04-05 2022-05-18 Quadram Inst Bioscience Determination and quantification of the microbial communities and antimicrobial resistance genes on food
CN114836510B (zh) * 2022-04-27 2025-05-13 武汉纺织大学 食品中细菌数量的快速检测方法和检测试剂盒
CN115628958B (zh) * 2022-11-21 2023-03-10 中检科(北京)测试认证有限公司 一种含有金刚乙胺的鸡蛋粉基质标准样品及其制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5368869A (en) 1993-03-09 1994-11-29 Utah State University Foundation Compositions and methods for manufacturing a skim or lowfat milk product with increased creaminess, color, mouthfeel, and taste sensations similar to milk with a higher fat content
US7077967B2 (en) * 2000-02-18 2006-07-18 Zentox Corporation Poultry processing water recovery and re-use process
WO2001079529A1 (en) * 2000-04-17 2001-10-25 Purdue Research Foundation Biosensor and related method
US20040248271A1 (en) * 2001-06-08 2004-12-09 Bu-Soo Park Composition for detecting peptidoglycan, and diagnostic kit detecting peptidoglycan
CA2559943A1 (en) * 2004-03-15 2005-10-06 Purdue Research Foundation Cell concentration and pathogen recovery
WO2007094077A1 (ja) * 2006-02-17 2007-08-23 Kyushu University 微生物検出法及び微生物検出キット
US20100226967A1 (en) * 2006-05-23 2010-09-09 Purdue Research Foundation Imaging and therapeutic method using progenitor cells
EP2831577B1 (en) 2012-03-28 2018-08-08 Purdue Research Foundation Methods and systems useful for foodborne pathogen detection

Also Published As

Publication number Publication date
US20180180611A1 (en) 2018-06-28
US11789018B2 (en) 2023-10-17
CA2991445A1 (en) 2017-01-26
WO2017015574A1 (en) 2017-01-26
CA2991445C (en) 2024-01-30
CN107923825A (zh) 2018-04-17
MX2018000840A (es) 2018-05-04

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