SG10201801064RA - System and method for on-site detection of pathogens - Google Patents

System and method for on-site detection of pathogens

Info

Publication number
SG10201801064RA
SG10201801064RA SG10201801064RA SG10201801064RA SG10201801064RA SG 10201801064R A SG10201801064R A SG 10201801064RA SG 10201801064R A SG10201801064R A SG 10201801064RA SG 10201801064R A SG10201801064R A SG 10201801064RA SG 10201801064R A SG10201801064R A SG 10201801064RA
Authority
SG
Singapore
Prior art keywords
pathogens
liquid sample
site
site detection
target species
Prior art date
Application number
SG10201801064RA
Inventor
Lee Chi Lik Ken
Yeong Chit Joel Chia
Original Assignee
Singapore Polytechnic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Singapore Polytechnic filed Critical Singapore Polytechnic
Priority to SG10201801064RA priority Critical patent/SG10201801064RA/en
Priority to PCT/SG2019/050062 priority patent/WO2019156626A1/en
Publication of SG10201801064RA publication Critical patent/SG10201801064RA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SYSTEM AND METHOD FOR ON-SITE DETECTION OF PATHOGENS 5 There is provided an on-site detection system for detecting pathogens in a liquid sample, the system comprising: a filtration module comprising a mixture of dead-end filtration elements and cross- flow filtration elements configured to obtain a sample population of pathogens from the liquid sample, an amplification module configured 10 to amplify one or more target biological elements present in one or more target species of pathogens in the sample population of pathogens; and a detection module configured to detect presence of amplified products of the one or more target biological elements present in the one or more target species of pathogens, wherein a 15 positive reading on the detection module indicates the presence of the one or more target species of pathogens. There is also provided an on-site method for detecting pathogens in a liquid sample. 20 Fig. 1
SG10201801064RA 2018-02-07 2018-02-07 System and method for on-site detection of pathogens SG10201801064RA (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
SG10201801064RA SG10201801064RA (en) 2018-02-07 2018-02-07 System and method for on-site detection of pathogens
PCT/SG2019/050062 WO2019156626A1 (en) 2018-02-07 2019-02-01 System and method for on-site detection of pathogens

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SG10201801064RA SG10201801064RA (en) 2018-02-07 2018-02-07 System and method for on-site detection of pathogens

Publications (1)

Publication Number Publication Date
SG10201801064RA true SG10201801064RA (en) 2019-09-27

Family

ID=67548079

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201801064RA SG10201801064RA (en) 2018-02-07 2018-02-07 System and method for on-site detection of pathogens

Country Status (2)

Country Link
SG (1) SG10201801064RA (en)
WO (1) WO2019156626A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110607354A (en) * 2019-10-31 2019-12-24 上海海洋大学 Rapid detection method of pathogenic vibrio cholerae
WO2023205648A1 (en) * 2022-04-18 2023-10-26 Godx, Inc. Use of exonuclease iii and probe for sensitive and specific lateral-flow-assay detection of amplification amplicons
CN116200509A (en) * 2022-08-17 2023-06-02 最高人民检察院检察技术信息研究中心 Food-borne pathogenic bacteria RPA nucleic acid isothermal amplification primer probe group, kit, detection method and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2018000840A (en) * 2015-07-23 2018-05-04 Purdue Research Foundation Rapid concentration, recovery and detection of pathogens in food samples.

Also Published As

Publication number Publication date
WO2019156626A1 (en) 2019-08-15

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