MX375786B - Biosintesis microbiana de ergotioneina. - Google Patents
Biosintesis microbiana de ergotioneina.Info
- Publication number
- MX375786B MX375786B MX2016014235A MX2016014235A MX375786B MX 375786 B MX375786 B MX 375786B MX 2016014235 A MX2016014235 A MX 2016014235A MX 2016014235 A MX2016014235 A MX 2016014235A MX 375786 B MX375786 B MX 375786B
- Authority
- MX
- Mexico
- Prior art keywords
- ergothioneine
- methods
- biosynthesis
- present disclosure
- microbial
- Prior art date
Links
- SSISHJJTAXXQAX-ZETCQYMHSA-N L-ergothioneine Chemical compound C[N+](C)(C)[C@H](C([O-])=O)CC1=CNC(=S)N1 SSISHJJTAXXQAX-ZETCQYMHSA-N 0.000 title abstract 5
- 229940093497 ergothioneine Drugs 0.000 title abstract 5
- 230000015572 biosynthetic process Effects 0.000 title abstract 4
- 230000000813 microbial effect Effects 0.000 title abstract 3
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención se refiere a métodos para la biosíntesis de ergotioneína; de manera más particular, la presente descripción se relaciona con métodos para biosíntesis microbiana de ergotioneína; la presente descripción se relaciona de manera general con célula hospederas manipuladas y métodos para producir ergotioneína; más particularmente, la presente descripción se relaciona con una célula hospedera manipulada y métodos para biosíntesis microbiana de ergotioneína utilizando la célula hospedera manipulada. Una célula hospedera manipulada por la producción de ergotioneína, la célula hospedera caracterizada porque comprende una vía metabólica para la producción de ergotioneína, en donde la célula hospedera es Escherichia coli, Sacharomyces cerevisiae, o Pichia pastoris, y en donde la célula hospedera comprende: una oxidorreductasa (EgtB) heteróloga que comprende una secuencia de aminoácidos que tiene por lo menos 99% de identidad de secuencia con la SEQ ID NO. 2, una antidohidrolasa (EgtC) heteróloga que comprende una secuencia aminoácidos que tiene por lo menos 99% de identidad de secuencia con la SEQ ID NO. 4, una histidina metiltransferasa (EgtD) heteróloga que comprende una secuencia de aminoácidos que tiene por lo menos 99% de identidad de secuencia con la SEQ ID NO: 6, y una proteína que une 5-fosfato de piridoxal (EgtE) heteróloga que comprende una secuencia de aminoácidos que tiene por lo menos99% de identidad de secuencia con la SEQ ID NO 8, en donde EgtB, EgtD y EgtE y hasta dos marcadores seleccionables son las únicas enzimas heterólogas en la célula hospedera.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985778P | 2014-04-29 | 2014-04-29 | |
| PCT/US2015/027977 WO2015168112A1 (en) | 2014-04-29 | 2015-04-28 | Microbial ergothioneine biosynthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2016014235A MX2016014235A (es) | 2017-02-14 |
| MX375786B true MX375786B (es) | 2025-03-07 |
Family
ID=54359235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016014235A MX375786B (es) | 2014-04-29 | 2015-04-28 | Biosintesis microbiana de ergotioneina. |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10544437B2 (es) |
| EP (1) | EP3137611B1 (es) |
| CN (1) | CN106661585B (es) |
| CA (1) | CA2975238C (es) |
| MX (1) | MX375786B (es) |
| WO (1) | WO2015168112A1 (es) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2975238C (en) | 2014-04-29 | 2021-05-11 | Conagen Inc. | Microbial ergothioneine biosynthesis |
| WO2017150304A1 (ja) * | 2016-02-29 | 2017-09-08 | 長瀬産業株式会社 | エルゴチオネインの発酵生産 |
| JP6948453B2 (ja) * | 2018-02-23 | 2021-10-13 | 長瀬産業株式会社 | エルゴチオネインの製造方法 |
| AU2020266254A1 (en) * | 2019-04-30 | 2021-12-23 | Danmarks Tekniske Universitet | Methods for production of ergothioneine |
| US20220281822A1 (en) | 2019-07-10 | 2022-09-08 | Kureha Corporation | Plant growth regulator and method for promoting plant growth |
| CN110358719B (zh) * | 2019-07-23 | 2021-05-04 | 江南大学 | 一种发酵合成麦角硫因的工程菌株及其构建方法 |
| CN110607286B (zh) * | 2019-08-21 | 2021-02-19 | 华南农业大学 | 灰树花麦角硫因基因Gfegt1和Gfegt2在合成麦角硫因中的应用 |
| CN110551697B (zh) * | 2019-08-26 | 2021-02-19 | 华南农业大学 | 侧耳类食用菌麦角硫因合成酶pegt1和pegt2在合成麦角硫因中的应用 |
| WO2021102736A1 (en) * | 2019-11-27 | 2021-06-03 | Nanjing Nutrabuilding Bio-Tech Co.,Ltd. | Ean B mutants and Their Uses |
| CN111004730A (zh) * | 2019-12-30 | 2020-04-14 | 江苏瑞霆生物科技有限公司 | 一种生产麦角硫因的方法 |
| US11732236B2 (en) * | 2020-01-09 | 2023-08-22 | Kureha Corporation | Method for producing novel microorganisms and ergothioneine |
| CN112251392B (zh) * | 2020-10-26 | 2022-09-09 | 天津科技大学 | 一种生产麦角硫因的基因工程菌株及其应用 |
| WO2022174177A1 (en) | 2021-02-15 | 2022-08-18 | Conagen Inc. | Microbial ergothioneine biosynthesis |
| CN114262702B (zh) * | 2021-12-31 | 2023-12-08 | 西南大学 | 麦角硫因合成基因在谷氨酸棒杆菌中重建麦角硫因代谢途径中的应用及其方法 |
| CN114854660B (zh) * | 2022-05-27 | 2024-03-26 | 深圳中科欣扬生物科技有限公司 | 一种高产麦角硫因的基因工程菌 |
| CN114854659B (zh) * | 2022-05-27 | 2024-03-26 | 深圳中科欣扬生物科技有限公司 | 一种麦角硫因生产工艺及其应用 |
| AR130564A1 (es) | 2022-09-27 | 2024-12-18 | Kureha Corp | Agente para mejorar la tolerancia al estrés ambiental en una planta y método para mejorar la tolerancia al estrés ambiental en una planta |
| CN118685371A (zh) * | 2023-03-23 | 2024-09-24 | 北京华熙荣熙生物技术研究有限公司 | 一种用于生产麦角硫因的亚砜合成酶及生物材料、生产方法与应用 |
| CN118685370A (zh) * | 2023-03-23 | 2024-09-24 | 北京华熙荣熙生物技术研究有限公司 | 一种亚砜合成酶突变体及其在麦角硫因生产中的应用 |
| CN119101636A (zh) * | 2023-06-09 | 2024-12-10 | 北京华熙荣熙生物技术研究有限公司 | 一种用于生产麦角硫因的菌株、生物材料、生产方法及应用 |
| CN117070479A (zh) * | 2023-08-17 | 2023-11-17 | 上海市农业科学院 | 一种生物合成麦角硫因相关基因及应用 |
| CN116970546B (zh) * | 2023-09-22 | 2023-12-26 | 江苏省中国科学院植物研究所 | 一种合成麦角硫因的工程菌株及其应用 |
| CN117904068B (zh) * | 2024-01-24 | 2025-04-25 | 江南大学 | 甲基转移酶突变体及其编码基因、载体、重组菌与应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2707087B1 (fr) | 1993-06-28 | 1995-10-13 | Bioxytech | Nouveau procédé de préparation de l'ergothionéine. |
| US6326034B1 (en) | 1999-12-22 | 2001-12-04 | Natural Compounds Ltd | Natural extracted and synthetic antioxidant compositions |
| CA2551537A1 (en) * | 2006-07-04 | 2008-01-04 | Institut Pasteur | Method for preparing recombinant mycobacterial polypeptides in yeast and their use in diagnosis of mycobacterial related diseases |
| US7767826B2 (en) | 2007-10-05 | 2010-08-03 | Pharmatech International, Inc. | Process for the synthesis of L-(+)-ergothioneine |
| FR2950889B1 (fr) | 2009-10-06 | 2011-11-18 | Tetrahedron | Procede de synthese de l'ergothioneine et analogues |
| CN103184246B (zh) | 2011-12-31 | 2017-03-22 | 中国科学院天津工业生物技术研究所 | 麦角硫因的生物合成制备方法 |
| CN103734022B (zh) * | 2012-10-16 | 2017-09-05 | 中国科学院天津工业生物技术研究所 | 生产麦角硫因的菌株及制备麦角硫因的方法 |
| CN104854245B (zh) * | 2012-12-21 | 2020-02-14 | 刘平华 | 通过代谢工程生产麦角硫因 |
| CA2975238C (en) | 2014-04-29 | 2021-05-11 | Conagen Inc. | Microbial ergothioneine biosynthesis |
-
2015
- 2015-04-28 CA CA2975238A patent/CA2975238C/en active Active
- 2015-04-28 EP EP15785908.3A patent/EP3137611B1/en active Active
- 2015-04-28 US US15/307,491 patent/US10544437B2/en active Active
- 2015-04-28 MX MX2016014235A patent/MX375786B/es active IP Right Grant
- 2015-04-28 WO PCT/US2015/027977 patent/WO2015168112A1/en not_active Ceased
- 2015-04-28 CN CN201580023207.3A patent/CN106661585B/zh active Active
-
2019
- 2019-12-13 US US16/714,659 patent/US20200224232A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20200224232A1 (en) | 2020-07-16 |
| CN106661585A (zh) | 2017-05-10 |
| CA2975238C (en) | 2021-05-11 |
| EP3137611B1 (en) | 2021-02-24 |
| EP3137611A4 (en) | 2018-03-07 |
| WO2015168112A1 (en) | 2015-11-05 |
| MX2016014235A (es) | 2017-02-14 |
| US10544437B2 (en) | 2020-01-28 |
| CN106661585B (zh) | 2021-04-09 |
| CA2975238A1 (en) | 2015-11-05 |
| EP3137611A1 (en) | 2017-03-08 |
| US20170051325A1 (en) | 2017-02-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |