MX373134B - Cepa recombinante de saccharomyces cerevisiae que sobreproduce glicerol. - Google Patents

Cepa recombinante de saccharomyces cerevisiae que sobreproduce glicerol.

Info

Publication number
MX373134B
MX373134B MX2016007642A MX2016007642A MX373134B MX 373134 B MX373134 B MX 373134B MX 2016007642 A MX2016007642 A MX 2016007642A MX 2016007642 A MX2016007642 A MX 2016007642A MX 373134 B MX373134 B MX 373134B
Authority
MX
Mexico
Prior art keywords
glycerol
recombinant strain
saccharomyces cerevisiae
overproduces
strain
Prior art date
Application number
MX2016007642A
Other languages
English (en)
Other versions
MX2016007642A (es
Inventor
Andriy Sibirny
Charles Abbas
Kostyantyn V Dmytruk
Lidiia R Murashchenko
Original Assignee
Archer Daniels Midland Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Archer Daniels Midland Co filed Critical Archer Daniels Midland Co
Publication of MX2016007642A publication Critical patent/MX2016007642A/es
Publication of MX373134B publication Critical patent/MX373134B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/102—Plasmid DNA for yeast
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a que se clonó una versión truncada del gen IVL2 de Saccharomyces cerevisiae que codifica una forma citosólica de la acetolactato sintasa, en un casete de expresión bajo el control de un promotor constitutivo fuerte del alcohol deshidrogenasa (ADH1). Se introdujo el plásmido en la cepa de S. cerevisiae y la cepa recombinante se ensayó para su capacidad de sobreproducir glicerol en condiciones anaeróbicas. Se demostró que la cepa recombinante se caracterizaba por una producción aumentada de glicerol y una producción disminuida de etanol en condiciones anaeróbicas.
MX2016007642A 2013-12-12 2014-12-05 Cepa recombinante de saccharomyces cerevisiae que sobreproduce glicerol. MX373134B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361915252P 2013-12-12 2013-12-12
PCT/US2014/068699 WO2015088892A2 (en) 2013-12-12 2014-12-05 Recombinant strain of saccharomyces cerevisiae overproducing glycerol

Publications (2)

Publication Number Publication Date
MX2016007642A MX2016007642A (es) 2017-02-20
MX373134B true MX373134B (es) 2020-06-15

Family

ID=53371950

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016007642A MX373134B (es) 2013-12-12 2014-12-05 Cepa recombinante de saccharomyces cerevisiae que sobreproduce glicerol.

Country Status (6)

Country Link
US (1) US20160304910A1 (es)
EP (1) EP3080283B1 (es)
BR (1) BR112016013467B1 (es)
CA (1) CA2932203C (es)
MX (1) MX373134B (es)
WO (1) WO2015088892A2 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111088177B (zh) * 2020-01-10 2023-04-25 淮北师范大学 高温好氧条件下产甘油的耐热酵母工程菌的构建及其应用
WO2022258733A1 (en) 2021-06-09 2022-12-15 Directsens Gmbh Novel production method of flavocytochrome b2

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8455239B2 (en) * 2007-12-23 2013-06-04 Gevo, Inc. Yeast organism producing isobutanol at a high yield
BRPI0909989A2 (pt) * 2008-06-05 2021-06-22 Butamax Advanced Biofuels Llc célula de levedura recombinante e método para a produção de um produto
US8795992B2 (en) * 2008-12-29 2014-08-05 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall integrity pathway
BR112012003241A2 (pt) * 2009-08-12 2015-09-01 Gevo Inc Localização de via de isobutanol citosólico para a produção de isobutanol
EP2783007B9 (en) * 2011-11-21 2018-03-28 Metabolic Explorer Microorganism strains for the production of 2,3-butanediol

Also Published As

Publication number Publication date
CA2932203A1 (en) 2015-06-18
CA2932203C (en) 2024-06-18
MX2016007642A (es) 2017-02-20
WO2015088892A3 (en) 2015-08-13
EP3080283A2 (en) 2016-10-19
BR112016013467B1 (pt) 2022-12-13
BR112016013467A2 (pt) 2018-01-16
WO2015088892A2 (en) 2015-06-18
EP3080283A4 (en) 2017-07-19
US20160304910A1 (en) 2016-10-20
EP3080283B1 (en) 2020-01-22

Similar Documents

Publication Publication Date Title
MY173141A (en) Glycerol and acetic acid converting yeast cells with improved acetic acid conversion
ES2721920T3 (es) Uso de fosfocetolasa y fosfotransacetilasa para la producción de compuestos derivados de acetil-coenzima A
BR112018007659A2 (pt) materiais de carbono com baixa gaseificação para aprimorar o desempenho de baterias de chumbo-ácido
MX347976B (es) Celulas huesped y metodos para producir isobutanol.
MX2015010875A (es) Microorganismo recombinante para utilizar en metodo con produccion aumentada de producto.
WO2014144705A3 (en) Microbial fuel cell and methods of use
BR112015021708A2 (pt) Inibidor de polipetídeo do tnfa, polinucleotídeo isolado, estrutura de expressão de ácido nucleico, célula de planta, composição farmacêutica e método de produção de um inibidor de polipeptídeo do tnfa
MX2012013037A (es) Composiciones de endorribonucleasa y metodos de uso de las mismas.
AR090575A1 (es) Agrobacterium para la transfeccion transitoria de plantas completas
EP3269003A4 (en) Design of smart-meas for high power fuel cells
CL2016001883A1 (es) Método para obtener cepas de levadura con producción de etanol baja, cepas de levadura obtenidas a partir de estas y su uso
MY168363A (en) Coupler for retaining an element relative to a retaining structure
WO2013087507A3 (en) Internally controllable fuel cell
BR112016002105A2 (pt) Microrganismo modificado, método para produzir alanina e uso de um microrganismo modificado
WO2013187974A3 (en) Aqueous assembly and control method
MX373134B (es) Cepa recombinante de saccharomyces cerevisiae que sobreproduce glicerol.
MX2016002616A (es) Microorganismo para la produccion de metionina con actividad de metionina sintasa y eflujo de metionina mejorados.
EA201391684A1 (ru) Электрическое восстановление анода твердого оксидного топливного элемента
WO2016005985A3 (en) Method for reprogramming cells
MY198268A (en) Omega-hydroxylase-related fusion polypeptides with improved properties
MX2017002662A (es) Metodo y microorganismo para produccion de metionina por fermentacion con eflujo de metionina mejorado.
BR112016027341A2 (pt) micro-organismo do gênero escherichia que tem um nível de atp intracelular aumentado e método para produzir l-aminoácidos
CA2886020C (en) Mutant yeast strain with decreased glycerol production
EP3084853A4 (en) Fuel pressure detection by fast magnetostrictive actuator
MX2016010130A (es) Un microorganismo modificado con capacidad de sintesis de biomasa mejorada y un metodo para el mismo campo.

Legal Events

Date Code Title Description
FG Grant or registration