MX366111B - Sistema y sustancias para la crioconservacion de celulas viables. - Google Patents
Sistema y sustancias para la crioconservacion de celulas viables.Info
- Publication number
- MX366111B MX366111B MX2014011005A MX2014011005A MX366111B MX 366111 B MX366111 B MX 366111B MX 2014011005 A MX2014011005 A MX 2014011005A MX 2014011005 A MX2014011005 A MX 2014011005A MX 366111 B MX366111 B MX 366111B
- Authority
- MX
- Mexico
- Prior art keywords
- cryopreservation
- fluid
- lipid
- compound
- surface energy
- Prior art date
Links
- 238000005138 cryopreservation Methods 0.000 title abstract 12
- 239000000126 substance Substances 0.000 title 1
- 239000012530 fluid Substances 0.000 abstract 9
- 150000002632 lipids Chemical class 0.000 abstract 6
- 150000001875 compounds Chemical class 0.000 abstract 4
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0623—Stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0626—Melanocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0661—Smooth muscle cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
- C12N5/0683—Cells of the male genital tract, e.g. prostate, epididymis; Non-germinal cells from testis, e.g. Leydig cells, Sertoli cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Gynecology & Obstetrics (AREA)
- Rheumatology (AREA)
- Dermatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Oncology (AREA)
- Virology (AREA)
- Hematology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere a un proceso para mejorar la crioconservación de células biológicas que comprende las etapas de: ensamblar un conjunto celular que contiene una pluralidad de estructuras celulares biológicas; establecer un fluido de crioconservación que contiene lípidos que va a incorporarse con dichas estructuras celulares biológicas en crioconservación; agregar al menos 270 J/kg de energía superficial a sustancialmente todos los dichos lípidos en dicho fluido de crioconservación que contiene lípidos para crear un fluido de crioconservación incrementado de energía superficial de lípidos, en donde dicho fluido de crioconservación incrementado de energía superficial de lídidos no es filtrado; mezclar sustancialmente todo el dicho fluido de crioconservación incrementado de energía superficial de lípidos y dichas estructuras celulares biológicas para formar un compuesto de crioconservación fluido incrementado de energía; retirar la energía térmica de dicho compuesto de crioconservación fluido; y congela dicho compuesto de croconservación fluido reduciendo la temperatura de dicho compuesto de crioconservación fluido por debajo del punto de congelación del agua.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261685224P | 2012-03-14 | 2012-03-14 | |
| PCT/US2013/030222 WO2013138239A1 (en) | 2012-03-14 | 2013-03-11 | System and substances for cryopreservation of viable cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014011005A MX2014011005A (es) | 2015-07-06 |
| MX366111B true MX366111B (es) | 2019-06-26 |
Family
ID=49161690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014011005A MX366111B (es) | 2012-03-14 | 2013-03-11 | Sistema y sustancias para la crioconservacion de celulas viables. |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US11028368B2 (es) |
| EP (1) | EP2825635B1 (es) |
| AU (1) | AU2013232381B2 (es) |
| BR (1) | BR112014022817A8 (es) |
| CA (1) | CA2905243C (es) |
| MX (1) | MX366111B (es) |
| WO (1) | WO2013138239A1 (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013138239A1 (en) | 2012-03-14 | 2013-09-19 | Membrane Protective Technologies, Inc. | System and substances for cryopreservation of viable cells |
| KR102106757B1 (ko) * | 2013-11-29 | 2020-05-06 | 주식회사 젬백스앤카엘 | 난소 동결 보존용 펩티드 및 이를 포함하는 조성물 |
| CN105532648B (zh) * | 2016-02-16 | 2018-01-12 | 云南省畜牧兽医科学院 | 一种小反刍动物精液冷冻稀释液 |
| WO2017160835A1 (en) | 2016-03-14 | 2017-09-21 | The Regents Of The University Of Michigan | A surface tension mediated lyo-processing technique for preservation of biologics |
| US10687525B2 (en) * | 2016-07-22 | 2020-06-23 | Tissue Testing Technologies Llc | Enhancement of cell cryopreservation with glycolipids |
| WO2019026910A1 (ja) * | 2017-07-31 | 2019-02-07 | 株式会社ツーセル | 凍結保存用組成物、凍結保存物の製造方法、細胞製剤、細胞製剤の製造方法、凍結保存用キット |
| BR112020010127A2 (pt) * | 2017-11-21 | 2020-11-10 | Membrane Protective Technologies, Inc. | métodos e sistemas para abordagem contínua e sinergística para tratamento e preservação de células biológicas. |
| CN108022285B (zh) * | 2017-11-30 | 2021-04-20 | 杭州电魂网络科技股份有限公司 | 地图渲染方法及装置 |
| CN111655258B (zh) * | 2017-12-31 | 2025-03-21 | 常青树生物科学公司 | 用于冷冻保存的组合物和其使用方法 |
| CN110810397A (zh) * | 2018-08-09 | 2020-02-21 | 湖北省生命源干细胞有限公司 | 一种造血干细胞冻存液及其制备方法和使用方法 |
| AU2019393877A1 (en) * | 2018-12-05 | 2021-06-17 | Seres Therapeutics, Inc. | Compositions for stabilizing bacteria and uses thereof |
| CA3133252A1 (en) * | 2019-03-13 | 2020-09-17 | Membrane Protective Technologies, Inc. | Methods and systems for protective supplementation during temperature depression |
| CN112358541B (zh) * | 2020-11-25 | 2022-04-01 | 广州迪澳医疗科技有限公司 | 一种重组人γ-干扰素的冻干保护剂 |
| CN112753695A (zh) * | 2021-01-04 | 2021-05-07 | 浙江卫未生物医药科技有限公司 | 一种免疫细胞冻存方法 |
| WO2023212529A1 (en) * | 2022-04-25 | 2023-11-02 | Abs Global, Inc. | Compositions and methods for enhancing sperm cell quality |
| WO2024243271A1 (en) * | 2023-05-23 | 2024-11-28 | Hatchless Inc. | Edible and food safe cryopreservation systems |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2793495B1 (fr) | 1999-05-14 | 2001-08-10 | Imv Technologies | Dilueur pour la cryoconservation de spermatozoides de bovins |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| WO2003022046A1 (en) | 2001-09-10 | 2003-03-20 | University Of Saskatchewan Technologies Inc. | Method for cryopreserving mammalian cells and tissues |
| BRPI0509485A (pt) | 2004-03-29 | 2007-09-11 | Monsanto Technology Llc | suspensões de esperma para uso em inseminação |
| WO2006079205A1 (en) | 2005-01-28 | 2006-08-03 | Canadian Blood Services | A method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability |
| US7622143B2 (en) | 2005-04-05 | 2009-11-24 | Membrane Protective Technologies, LLC | Reproductive cell system |
| US9919014B2 (en) | 2005-04-05 | 2018-03-20 | Membrane Protective Technologies, Inc. | Reproductive cell maintenance system |
| EP1981987A2 (en) * | 2006-01-04 | 2008-10-22 | Do-Coop Technologies Ltd | Compositions and methods for enhancing in-vivo uptake of pharmaceutical agents |
| WO2007138119A1 (es) | 2006-05-30 | 2007-12-06 | Instituto Nacional De Investigación Y Tecnología Agraria Y Alimentaria, Inia | Procedimiento de preparación de vesículas unilamelares para la criopreservación y cultivo de celulas germinales y embriones |
| ES2615390T3 (es) | 2008-03-05 | 2017-06-06 | Sanofi Pasteur | Proceso para estabilizar una composición de vacuna que contiene adyuvante |
| US20100311036A1 (en) | 2009-06-09 | 2010-12-09 | University Of South Carolina | Methods for Augmentation of Cell Cryopreservation |
| WO2013138239A1 (en) | 2012-03-14 | 2013-09-19 | Membrane Protective Technologies, Inc. | System and substances for cryopreservation of viable cells |
-
2013
- 2013-03-11 WO PCT/US2013/030222 patent/WO2013138239A1/en not_active Ceased
- 2013-03-11 CA CA2905243A patent/CA2905243C/en active Active
- 2013-03-11 US US14/385,471 patent/US11028368B2/en active Active
- 2013-03-11 MX MX2014011005A patent/MX366111B/es active IP Right Grant
- 2013-03-11 AU AU2013232381A patent/AU2013232381B2/en active Active
- 2013-03-11 BR BR112014022817A patent/BR112014022817A8/pt not_active Application Discontinuation
- 2013-03-11 EP EP13760949.1A patent/EP2825635B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2013232381B2 (en) | 2018-08-30 |
| WO2013138239A1 (en) | 2013-09-19 |
| BR112014022817A8 (pt) | 2021-07-13 |
| AU2013232381A1 (en) | 2014-10-30 |
| CA2905243A1 (en) | 2013-09-19 |
| EP2825635A1 (en) | 2015-01-21 |
| EP2825635B1 (en) | 2020-04-15 |
| BR112014022817A2 (pt) | 2017-06-20 |
| EP2825635A4 (en) | 2015-09-23 |
| US20150037783A1 (en) | 2015-02-05 |
| MX2014011005A (es) | 2015-07-06 |
| AU2013232381A2 (en) | 2014-11-20 |
| CA2905243C (en) | 2021-11-09 |
| US11028368B2 (en) | 2021-06-08 |
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