MX2022011418A - Métodos y composiciones para el silenciamiento de génes basado en crispr tipo i-e mejorado. - Google Patents
Métodos y composiciones para el silenciamiento de génes basado en crispr tipo i-e mejorado.Info
- Publication number
- MX2022011418A MX2022011418A MX2022011418A MX2022011418A MX2022011418A MX 2022011418 A MX2022011418 A MX 2022011418A MX 2022011418 A MX2022011418 A MX 2022011418A MX 2022011418 A MX2022011418 A MX 2022011418A MX 2022011418 A MX2022011418 A MX 2022011418A
- Authority
- MX
- Mexico
- Prior art keywords
- compositions
- methods
- gene silencing
- based gene
- improved type
- Prior art date
Links
- 108091033409 CRISPR Proteins 0.000 title abstract 3
- 230000030279 gene silencing Effects 0.000 title abstract 2
- 238000012226 gene silencing method Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 238000010354 CRISPR gene editing Methods 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 101100005249 Escherichia coli (strain K12) ygcB gene Proteins 0.000 abstract 1
- 108020005004 Guide RNA Proteins 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 238000003491 array Methods 0.000 abstract 1
- 101150055191 cas3 gene Proteins 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
La interferencia basada en CRISPR se ha vuelto común en varias aplicaciones de circuitos genéticos para el control metabólico dinámico. En E. coli, el sistema nativo de Cascada de CRISPR se puede utilizar para el silenciamiento mediante la deleción de la nucleasa cas3 junto con la expresión de series de ARN guías, donde se pueden silenciar múltiples genes a partir de un solo transcrito.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062990172P | 2020-03-16 | 2020-03-16 | |
PCT/US2021/022583 WO2021188554A2 (en) | 2020-03-16 | 2021-03-16 | Methods and compositions for improved type i-e crispr based gene silencing |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022011418A true MX2022011418A (es) | 2022-12-15 |
Family
ID=77768376
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022011418A MX2022011418A (es) | 2020-03-16 | 2021-03-16 | Métodos y composiciones para el silenciamiento de génes basado en crispr tipo i-e mejorado. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20230193264A1 (es) |
EP (1) | EP4103689A4 (es) |
JP (1) | JP2023518028A (es) |
KR (1) | KR20220154748A (es) |
CN (1) | CN115461441A (es) |
AU (1) | AU2021239868A1 (es) |
BR (1) | BR112022018344A2 (es) |
CA (1) | CA3175878A1 (es) |
IL (1) | IL296416A (es) |
MX (1) | MX2022011418A (es) |
WO (1) | WO2021188554A2 (es) |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2017512481A (ja) * | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
GB2528177B (en) * | 2014-06-11 | 2019-08-28 | Univ Duke | Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves |
SG11201801809VA (en) * | 2015-09-09 | 2018-04-27 | Univ Kobe Nat Univ Corp | Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method |
JP6878607B2 (ja) * | 2017-02-21 | 2021-05-26 | デューク ユニバーシティ | ロバストな動的な代謝コントロールの為の組成物及び方法 |
US10227576B1 (en) * | 2018-06-13 | 2019-03-12 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
-
2021
- 2021-03-16 AU AU2021239868A patent/AU2021239868A1/en active Pending
- 2021-03-16 IL IL296416A patent/IL296416A/en unknown
- 2021-03-16 KR KR1020227035523A patent/KR20220154748A/ko unknown
- 2021-03-16 WO PCT/US2021/022583 patent/WO2021188554A2/en unknown
- 2021-03-16 JP JP2022555128A patent/JP2023518028A/ja active Pending
- 2021-03-16 CA CA3175878A patent/CA3175878A1/en active Pending
- 2021-03-16 BR BR112022018344A patent/BR112022018344A2/pt unknown
- 2021-03-16 US US17/906,372 patent/US20230193264A1/en active Pending
- 2021-03-16 EP EP21772647.0A patent/EP4103689A4/en active Pending
- 2021-03-16 MX MX2022011418A patent/MX2022011418A/es unknown
- 2021-03-16 CN CN202180030391.XA patent/CN115461441A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
EP4103689A2 (en) | 2022-12-21 |
CA3175878A1 (en) | 2021-09-23 |
BR112022018344A2 (pt) | 2022-11-08 |
US20230193264A1 (en) | 2023-06-22 |
JP2023518028A (ja) | 2023-04-27 |
EP4103689A4 (en) | 2023-08-02 |
IL296416A (en) | 2022-11-01 |
WO2021188554A2 (en) | 2021-09-23 |
AU2021239868A1 (en) | 2022-10-06 |
WO2021188554A3 (en) | 2021-12-02 |
CN115461441A (zh) | 2022-12-09 |
KR20220154748A (ko) | 2022-11-22 |
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