MX2022008653A - Metodos para el analisis glucoproteomico de celulas enteras. - Google Patents

Metodos para el analisis glucoproteomico de celulas enteras.

Info

Publication number
MX2022008653A
MX2022008653A MX2022008653A MX2022008653A MX2022008653A MX 2022008653 A MX2022008653 A MX 2022008653A MX 2022008653 A MX2022008653 A MX 2022008653A MX 2022008653 A MX2022008653 A MX 2022008653A MX 2022008653 A MX2022008653 A MX 2022008653A
Authority
MX
Mexico
Prior art keywords
methods
whole
cell
analysis
membrane
Prior art date
Application number
MX2022008653A
Other languages
English (en)
Inventor
Yuan Mao
Shruti Nayak
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022008653A publication Critical patent/MX2022008653A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La presente descripción se refiere a la glucoproteómica. Más específicamente, la presente descripción proporciona métodos para determinar una o más de las glucoproteínas, sitios de glucosilación, fragmentos de glucopéptidos, y composiciones de glucanos tanto de las proteínas de membrana como citosólicas. Los métodos en la presente descripción emplean un único método de procesamiento que permite la extracción proteínas de membrana y citosólicas para la identificación y análisis de la glucosilación de células enteras, independientemente de la especie o el tipo de muestra.
MX2022008653A 2020-01-31 2021-01-29 Metodos para el analisis glucoproteomico de celulas enteras. MX2022008653A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062968536P 2020-01-31 2020-01-31
PCT/US2021/015601 WO2021155077A1 (en) 2020-01-31 2021-01-29 Methods for whole-cell glycoproteomic analysis

Publications (1)

Publication Number Publication Date
MX2022008653A true MX2022008653A (es) 2022-08-10

Family

ID=74858749

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022008653A MX2022008653A (es) 2020-01-31 2021-01-29 Metodos para el analisis glucoproteomico de celulas enteras.

Country Status (11)

Country Link
US (1) US20210239706A1 (es)
EP (1) EP4097482A1 (es)
JP (1) JP2023512401A (es)
KR (1) KR20220134516A (es)
CN (1) CN115151823A (es)
AU (1) AU2021213774A1 (es)
BR (1) BR112022010499A2 (es)
CA (1) CA3154703A1 (es)
IL (1) IL294843A (es)
MX (1) MX2022008653A (es)
WO (1) WO2021155077A1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010071817A2 (en) * 2008-12-19 2010-06-24 Momenta Pharmaceuticals, Inc. Characterization of o-linked glycans
US9410966B2 (en) * 2013-01-17 2016-08-09 The Regents Of The University Of California Isotopic recoding for targeted tandem mass spectrometry

Also Published As

Publication number Publication date
CA3154703A1 (en) 2021-08-05
IL294843A (en) 2022-09-01
CN115151823A (zh) 2022-10-04
AU2021213774A1 (en) 2022-06-16
WO2021155077A1 (en) 2021-08-05
JP2023512401A (ja) 2023-03-27
EP4097482A1 (en) 2022-12-07
US20210239706A1 (en) 2021-08-05
BR112022010499A2 (pt) 2022-09-06
KR20220134516A (ko) 2022-10-05

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