MX2022008323A - Uso de aminoácidos para mejorar la señal en análisis del espectro de masas. - Google Patents

Uso de aminoácidos para mejorar la señal en análisis del espectro de masas.

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Publication number
MX2022008323A
MX2022008323A MX2022008323A MX2022008323A MX2022008323A MX 2022008323 A MX2022008323 A MX 2022008323A MX 2022008323 A MX2022008323 A MX 2022008323A MX 2022008323 A MX2022008323 A MX 2022008323A MX 2022008323 A MX2022008323 A MX 2022008323A
Authority
MX
Mexico
Prior art keywords
small molecule
amino acid
mass spectral
molecule additive
separation column
Prior art date
Application number
MX2022008323A
Other languages
English (en)
Inventor
Lili Guo
Yuan Mao
Andrew Kleinberg
Yunlong Zhao
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022008323A publication Critical patent/MX2022008323A/es

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se describe un método para mejorar una señal del espectro de masas. El método puede incluir poner en contacto una muestra con una columna de separación en condiciones que permitan que los componentes de la muestra se unan al sustrato; aplicarle un primer gradiente móvil a la columna de separación, en donde el gradiente de primera fase móvil comprende ácido trifluoroacético (TFA) y un aditivo de moléculas pequeñas (p. ej., un aminoácido) o ácido fórmico (FA) y un aditivo de moléculas pequeñas (p. ej., un aminoácido); aplicarle un segundo gradiente móvil a la columna de separación, en donde el gradiente de segunda fase móvil comprende TFA en acetonitrilo (ACN) y un aditivo de moléculas pequeñas (p. ej., un aminoácido) o ácido fórmico (FA) en ACN y un aditivo de moléculas pequeñas (p. ej., un aminoácido); y realizar un análisis de espectrometría de masas en componentes de la muestra eluidos.
MX2022008323A 2020-01-08 2021-01-07 Uso de aminoácidos para mejorar la señal en análisis del espectro de masas. MX2022008323A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062958366P 2020-01-08 2020-01-08
US202063053836P 2020-07-20 2020-07-20
PCT/US2021/012523 WO2021142137A1 (en) 2020-01-08 2021-01-07 Use of amino acids to enhance signal in mass spectral analyses

Publications (1)

Publication Number Publication Date
MX2022008323A true MX2022008323A (es) 2022-08-08

Family

ID=74669507

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022008323A MX2022008323A (es) 2020-01-08 2021-01-07 Uso de aminoácidos para mejorar la señal en análisis del espectro de masas.

Country Status (11)

Country Link
US (2) US20210223259A1 (es)
EP (1) EP4088120A1 (es)
JP (1) JP2023509759A (es)
KR (1) KR20220123116A (es)
CN (1) CN115280151A (es)
AU (1) AU2021205910A1 (es)
BR (1) BR112022013481A2 (es)
CA (1) CA3163987A1 (es)
IL (1) IL294319A (es)
MX (1) MX2022008323A (es)
WO (1) WO2021142137A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3202429A1 (en) * 2020-12-20 2022-06-23 Regeneron Pharmaceuticals, Inc. Methods for identification of scrambled disulfides in biomolecules
JP7487987B1 (ja) 2023-12-21 2024-05-21 伊那食品工業株式会社 アガロオリゴ糖の分析方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5851400A (en) * 1997-03-21 1998-12-22 University Of Maryland At Baltimore County Method for eliminating the displacer in displacement chromatography of proteins
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
EP1558354B1 (en) * 2002-10-30 2010-02-17 Merck & Co., Inc. Using amines, amino acids or amino acid esters as mobile phase modifiers in chromatography
CA2751000A1 (en) * 2009-03-11 2010-12-23 Wyeth Llc Methods of purifying small modular immunopharmaceutical proteins
CN103833855A (zh) 2009-06-26 2014-06-04 瑞泽恩制药公司 容易地分离的具有天然免疫球蛋白形式的双特异性抗体
EP2844373A4 (en) * 2012-05-03 2015-12-16 Medimmune Llc METHOD FOR ANALYZING SAMPLE COMPONENTS
CN105980857B (zh) * 2013-11-15 2018-07-13 奥塔哥创新有限公司 用于心脏病症的生物标记物

Also Published As

Publication number Publication date
CA3163987A1 (en) 2021-07-15
AU2021205910A1 (en) 2022-09-01
CN115280151A (zh) 2022-11-01
KR20220123116A (ko) 2022-09-05
IL294319A (en) 2022-08-01
US20210223259A1 (en) 2021-07-22
EP4088120A1 (en) 2022-11-16
US20230417759A1 (en) 2023-12-28
WO2021142137A1 (en) 2021-07-15
JP2023509759A (ja) 2023-03-09
BR112022013481A2 (pt) 2022-09-13

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