MX2022000735A - Metodos para el analisis del adn microbiano. - Google Patents

Metodos para el analisis del adn microbiano.

Info

Publication number
MX2022000735A
MX2022000735A MX2022000735A MX2022000735A MX2022000735A MX 2022000735 A MX2022000735 A MX 2022000735A MX 2022000735 A MX2022000735 A MX 2022000735A MX 2022000735 A MX2022000735 A MX 2022000735A MX 2022000735 A MX2022000735 A MX 2022000735A
Authority
MX
Mexico
Prior art keywords
methods
intact cells
dna analysis
microbial dna
dna
Prior art date
Application number
MX2022000735A
Other languages
English (en)
Inventor
Michael Edward Hogan
Benjamin Alan Katchman
Candy Mavis Rivas
Yasmine Eve Baca
Original Assignee
PathogenDX Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PathogenDX Inc filed Critical PathogenDX Inc
Publication of MX2022000735A publication Critical patent/MX2022000735A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/32—Centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/31—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.1.31)
    • C12Y301/31001—Micrococcal nuclease (3.1.31.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente descripción se proporcionan métodos para aislar ácidos nucleicos de células intactas en una muestra de células intactas, células muertas contaminantes, restos celulares y biopelícula mediante el uso dos etapas de separación, ya sea por centrifugación o filtración, realizadas en forma secuencial. También se proporciona un método para aislar ácidos nucleicos de células intactas mediante el uso de una primera etapa de separación seguido de tratamiento con una nucleasa y luego una segunda etapa de separación. En la presente descripción se proporciona un método relacionado para aislar ADN de células intactas mediante el uso de una nucleasa que produce cortes de ADN en ADN bicatenario, seguido de una segunda etapa de separación.
MX2022000735A 2019-07-19 2020-07-16 Metodos para el analisis del adn microbiano. MX2022000735A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962876054P 2019-07-19 2019-07-19
US16/816,481 US11319582B2 (en) 2019-07-19 2020-03-12 Methods for microbial DNA analysis
PCT/US2020/042321 WO2021016036A1 (en) 2019-07-19 2020-07-16 Methods for microbial dna analysis

Publications (1)

Publication Number Publication Date
MX2022000735A true MX2022000735A (es) 2022-07-12

Family

ID=74194087

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022000735A MX2022000735A (es) 2019-07-19 2020-07-16 Metodos para el analisis del adn microbiano.

Country Status (6)

Country Link
US (1) US11319582B2 (es)
EP (1) EP3999656A4 (es)
CN (1) CN114787377A (es)
CA (1) CA3144918A1 (es)
MX (1) MX2022000735A (es)
WO (1) WO2021016036A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115417510B (zh) * 2022-09-20 2023-07-04 南京农业大学 一种水中胞外抗生素抗性基因的去除方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020172972A1 (en) * 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
DE102005009479A1 (de) * 2005-03-02 2006-09-07 Molzym Gmbh & Co. Kg Verwendung von Nukleasen zum Abbau von Nukleinsäure in Gegenwart von chaotropen Agenzien und/oder Tensiden
CA2862523A1 (en) * 2010-12-31 2012-07-05 Zeus Scientific, Inc. Improved methods for determining cell viability using molecular nucleic acid-based techniques
WO2019116034A1 (en) * 2017-12-12 2019-06-20 Cell Therapy Catapult Limited Microbial enrichment method

Also Published As

Publication number Publication date
US20210017590A1 (en) 2021-01-21
EP3999656A4 (en) 2023-08-23
EP3999656A1 (en) 2022-05-25
CA3144918A1 (en) 2021-01-28
US11319582B2 (en) 2022-05-03
CN114787377A (zh) 2022-07-22
WO2021016036A1 (en) 2021-01-28

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