MX2021011837A - Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida. - Google Patents

Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida.

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Publication number
MX2021011837A
MX2021011837A MX2021011837A MX2021011837A MX2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A MX 2021011837 A MX2021011837 A MX 2021011837A
Authority
MX
Mexico
Prior art keywords
fcrn
expression cassette
cassette encoding
generation
expression cassettes
Prior art date
Application number
MX2021011837A
Other languages
English (en)
Inventor
Stefan Seeber
Tilman Schlothauer
Jasmin Maria Wehrstein
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2021011837A publication Critical patent/MX2021011837A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y603/00—Ligases forming carbon-nitrogen bonds (6.3)
    • C12Y603/04—Other carbon-nitrogen ligases (6.3.4)
    • C12Y603/04015—Biotin-[acetyl-CoA-carboxylase] ligase (6.3.4.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag

Abstract

En el presente documento se informa de un método para producir el FcRn biotinilado en el extremo C-terminal que comprende los pasos de cultivar una célula de mamífero que comprende un ácido desoxirribonucleico que codifica el FcRn y la ligasa biotina-[acetil-CoA-carboxilasa] (BirA) de E. coli en un medio que contiene biotina, y recuperar el FcRn biotinilado en el extremo C-terminal de la célula o del medio de cultivo, en donde el ácido desoxirribonucleico que codifica el FcRn y la BirA de E. coli está integrado de manera estable en el genoma de la célula de mamífero y comprende en la dirección 5' a 3' un primer casete de expresión que codifica la proteína similar al complejo principal de histocompatibilidad clase I (a-FcRn) que comprende una etiqueta HisAvi en el extremo C-terminal, un segundo casete de expresión que codifica la ß2-microglobulina (ß2m), un tercer casete de expresión que codifica la proteína similar al complejo principal de histocompatibilidad clase I (a-FcRn) que comprende una etiqueta HisAvi en el extremo C-terminal, un cuarto casete de expresión que codifica la ß2-microglobulina (ß2m), y un quinto casete de expresión que codifica la ligasa biotina-[acetil-CoA-car boxilasa] de E.
MX2021011837A 2019-03-29 2020-03-26 Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida. MX2021011837A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19166030 2019-03-29
PCT/EP2020/058452 WO2020200983A1 (en) 2019-03-29 2020-03-26 Method for the generation of an fcrn expressing cell by targeted integration of multiple expression cassettes in a defined organization

Publications (1)

Publication Number Publication Date
MX2021011837A true MX2021011837A (es) 2021-10-22

Family

ID=66239740

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021011837A MX2021011837A (es) 2019-03-29 2020-03-26 Método para la generación de una célula que expresa el receptor neonatal de región de fragmento cristalizable (fcrn) mediante la integración dirigida de múltiples casetes de expresión en una organización definida.

Country Status (12)

Country Link
US (1) US20220119482A1 (es)
EP (1) EP3947437A1 (es)
JP (1) JP7250950B2 (es)
KR (1) KR20210134932A (es)
CN (1) CN113661173A (es)
AU (1) AU2020253023B2 (es)
BR (1) BR112021019368A2 (es)
CA (1) CA3130546A1 (es)
IL (1) IL286718A (es)
MX (1) MX2021011837A (es)
SG (1) SG11202109391PA (es)
WO (1) WO2020200983A1 (es)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0557459B1 (en) 1990-11-13 1997-10-22 Immunex Corporation Bifunctional selectable fusion genes
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
BRPI0513706A (pt) 2004-07-20 2008-05-13 Symphogen As anticorpo policlonal recombinante anti-rhesus d e métodos de produção
WO2007124452A2 (en) * 2006-04-20 2007-11-01 The Regents Of The University Of California Methods for expressing multiple sirna and shrna from a single vector
US8293533B2 (en) * 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
WO2013006142A1 (en) 2011-07-05 2013-01-10 Nanyang Technological University A novel process and reagent for rapid genetic alterations in eukaryotic cells
JP6152120B2 (ja) 2012-02-15 2017-06-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Ｆｃ受容体に基づくアフィニティークロマトグラフィー
MX2015013842A (es) * 2013-03-30 2017-01-23 Usha Biotech Ltd Metodos y constructos para expresar proteinas biologicamente activas en celulas de mamifero.
HK1218486A2 (zh) * 2015-12-09 2017-02-17 Ka Lun Alan Ng 提高魚生長的手段和方法
WO2017184831A1 (en) * 2016-04-20 2017-10-26 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018162517A1 (en) 2017-03-10 2018-09-13 F. Hoffmann-La Roche Ag Method for producing multispecific antibodies
WO2018197533A1 (en) * 2017-04-28 2018-11-01 F. Hoffmann-La Roche Ag Antibody selection method
AU2018392728A1 (en) 2017-12-22 2020-07-09 Genentech, Inc. Targeted integration of nucleic acids

Also Published As

Publication number Publication date
JP7250950B2 (ja) 2023-04-03
BR112021019368A2 (pt) 2021-12-07
AU2020253023B2 (en) 2022-07-14
US20220119482A1 (en) 2022-04-21
CN113661173A (zh) 2021-11-16
CA3130546A1 (en) 2020-10-08
AU2020253023A1 (en) 2021-09-23
KR20210134932A (ko) 2021-11-11
SG11202109391PA (en) 2021-09-29
IL286718A (en) 2021-10-31
WO2020200983A1 (en) 2020-10-08
JP2022526556A (ja) 2022-05-25
EP3947437A1 (en) 2022-02-09

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