MX2021007777A - Ensayos de colonizacion de plantas mediante el uso de codigos de barras microbianos naturales. - Google Patents

Ensayos de colonizacion de plantas mediante el uso de codigos de barras microbianos naturales.

Info

Publication number
MX2021007777A
MX2021007777A MX2021007777A MX2021007777A MX2021007777A MX 2021007777 A MX2021007777 A MX 2021007777A MX 2021007777 A MX2021007777 A MX 2021007777A MX 2021007777 A MX2021007777 A MX 2021007777A MX 2021007777 A MX2021007777 A MX 2021007777A
Authority
MX
Mexico
Prior art keywords
barcodes
cells
sites
nucleic acid
present disclosure
Prior art date
Application number
MX2021007777A
Other languages
English (en)
Inventor
Douglas Higgins
Andrew Abel Prior
Original Assignee
Pivot Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of MX2021007777A publication Critical patent/MX2021007777A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/025—Achromobacter
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22—Klebsiella

Abstract

La presente descripción se refiere a métodos para utilizar códigos de barras de ácido nucleico y sitios de amplificación correspondientes en células en las que los códigos de barras se encuentran de forma natural. Estos códigos de barras y sitios de amplificación se reconfiguran en un único casete de ácido nucleico que facilita su uso en el etiquetado de especies, cepas o variantes de células particulares, cada una con un código de barras diferente. Estos códigos de barras pueden usarse para rastrear las capacidades de colonización de las células con códigos de barras. La presente descripción proporciona, además, ensayos que utilizan códigos de barras naturales para medir la capacidad relativa de colonización microbiana de un sistema de raíces de plantas.
MX2021007777A 2019-01-07 2020-01-07 Ensayos de colonizacion de plantas mediante el uso de codigos de barras microbianos naturales. MX2021007777A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962789332P 2019-01-07 2019-01-07
US201962906419P 2019-09-26 2019-09-26
PCT/US2020/012564 WO2020146372A1 (en) 2019-01-07 2020-01-07 Plant colonization assays using natural microbial barcodes

Publications (1)

Publication Number Publication Date
MX2021007777A true MX2021007777A (es) 2021-10-13

Family

ID=71520864

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021007777A MX2021007777A (es) 2019-01-07 2020-01-07 Ensayos de colonizacion de plantas mediante el uso de codigos de barras microbianos naturales.

Country Status (9)

Country Link
US (1) US20220090095A1 (es)
EP (1) EP3908660A4 (es)
CN (1) CN114929874A (es)
AU (1) AU2020206751A1 (es)
BR (1) BR112021013270A2 (es)
CA (1) CA3123940A1 (es)
MX (1) MX2021007777A (es)
UY (1) UY38538A (es)
WO (1) WO2020146372A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
MX2018000615A (es) 2015-07-13 2018-08-01 Pivot Bio Inc Metodos y composiciones para mejorar atributos de plantas.
CA3001001A1 (en) 2015-10-05 2017-04-13 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
AU2018207204B2 (en) 2017-01-12 2023-11-30 Pivot Bio, Inc. Methods and compositions for improving plant traits
BR112020026771A2 (pt) 2018-06-27 2021-03-30 Pivot Bio, Inc. Composições agrícolas que compreendem micróbios de fixação de nitrogênio remodelados
CN112899188A (zh) * 2021-01-29 2021-06-04 西南大学 一种促进作物根系发育的微生物菌剂及其制备与应用
CN113284560B (zh) * 2021-04-28 2022-05-17 广州微远基因科技有限公司 病原检测背景微生物判断方法及应用
WO2023077095A2 (en) * 2021-10-29 2023-05-04 Mammoth Biosciences, Inc. Effector proteins, compositions, systems, devices, kits and methods of use thereof
CN115253453A (zh) * 2022-08-12 2022-11-01 海南河道综合整治工程有限公司 一种节水砂料冲洗装置
CN115747358B (zh) * 2022-11-19 2023-08-18 河南农业大学 一种鉴定pgpr趋化根系分泌物到达根际的趋化速率的方法及其应用
CN116694655A (zh) * 2023-05-15 2023-09-05 浙江大学 一个陆地棉油分蛋白品质关联的葡萄糖-甲醇-胆碱氧化还原酶基因的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2016348439B2 (en) * 2015-11-04 2023-03-09 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
AU2018207204B2 (en) * 2017-01-12 2023-11-30 Pivot Bio, Inc. Methods and compositions for improving plant traits

Also Published As

Publication number Publication date
CN114929874A (zh) 2022-08-19
AU2020206751A1 (en) 2021-07-01
UY38538A (es) 2020-06-30
EP3908660A4 (en) 2022-11-02
US20220090095A1 (en) 2022-03-24
WO2020146372A1 (en) 2020-07-16
CA3123940A1 (en) 2020-07-16
WO2020146372A9 (en) 2020-09-10
BR112021013270A2 (pt) 2021-11-23
EP3908660A1 (en) 2021-11-17

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