MX2021002672A - Proceso mejorado para la preparación de proteína recombinante. - Google Patents

Proceso mejorado para la preparación de proteína recombinante.

Info

Publication number
MX2021002672A
MX2021002672A MX2021002672A MX2021002672A MX2021002672A MX 2021002672 A MX2021002672 A MX 2021002672A MX 2021002672 A MX2021002672 A MX 2021002672A MX 2021002672 A MX2021002672 A MX 2021002672A MX 2021002672 A MX2021002672 A MX 2021002672A
Authority
MX
Mexico
Prior art keywords
recombinant lectin
preparation
improved process
lectin protein
seq
Prior art date
Application number
MX2021002672A
Other languages
English (en)
Inventor
Dhananjay Sathe
Sudeep Kumar
Mamata Katdare
Original Assignee
Unichem Lab Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unichem Lab Ltd filed Critical Unichem Lab Ltd
Publication of MX2021002672A publication Critical patent/MX2021002672A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4726—Lectins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Water Supply & Treatment (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere al proceso para preparar lectina recombinante que tiene la secuencia de aminoácidos de la SEQ ID NO: 1, en donde el proceso comprende la fermentación discontinua alimentada de un clon en una célula huésped a una velocidad de alimentación específica con una relación de carbono a nitrógeno de 3:1 a 6:1. La invención también se refiere al proceso de purificación de lectina recombinante que tiene la secuencia de aminoácidos de la SEQ ID NO: 1.
MX2021002672A 2018-09-07 2019-09-05 Proceso mejorado para la preparación de proteína recombinante. MX2021002672A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IN201821008382 2018-09-07
IN201921001559 2019-01-14
PCT/IB2019/057471 WO2020074977A1 (en) 2018-09-07 2019-09-05 An improved process for the preparation of recombinant lectin protein

Publications (1)

Publication Number Publication Date
MX2021002672A true MX2021002672A (es) 2021-06-23

Family

ID=68159140

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021002672A MX2021002672A (es) 2018-09-07 2019-09-05 Proceso mejorado para la preparación de proteína recombinante.

Country Status (13)

Country Link
US (1) US20210324438A1 (es)
EP (1) EP3847184A1 (es)
JP (1) JP2021536253A (es)
KR (1) KR20210058840A (es)
CN (1) CN113286809A (es)
AU (1) AU2019358330B2 (es)
BR (1) BR112021004241A2 (es)
CA (1) CA3111367A1 (es)
MA (1) MA53554A (es)
MX (1) MX2021002672A (es)
SG (1) SG11202102209XA (es)
WO (1) WO2020074977A1 (es)
ZA (1) ZA202101433B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202104787PA (en) * 2018-11-19 2021-06-29 Unichem Lab Ltd Combination of chemotherapy with recombinant s. rolfsii lectin
US20240325491A1 (en) 2021-07-08 2024-10-03 Unichem Laboratories Ltd Recombinant proteins, compositions and methods of stabilization thereof
AU2022354289A1 (en) 2021-10-01 2024-03-14 Unichem Laboratories Limited Lectin-drug conjugates

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100339475C (zh) * 2003-08-14 2007-09-26 海南大学 用于重组生产雪花莲外源凝集素的大肠杆菌菌株及方法
SI2430041T1 (sl) * 2009-02-18 2019-04-30 Unichen Laboratories Limited Unichem Bhavan, Prabhat Estate Rekombinantni lektini, ki vežejo rakaste celice, z antitumorskim delovanjem in postopek priprave
WO2014203261A2 (en) * 2010-02-02 2014-12-24 Unichem Laboratories Ltd. Recombinant lectin and method of preparing thereof
US10435732B2 (en) * 2014-06-09 2019-10-08 Kaneka Corporation Method for producing recombinant proteins using recombinant brevibacillus

Also Published As

Publication number Publication date
KR20210058840A (ko) 2021-05-24
MA53554A (fr) 2021-07-14
CN113286809A (zh) 2021-08-20
BR112021004241A2 (pt) 2021-05-25
SG11202102209XA (en) 2021-04-29
CA3111367A1 (en) 2020-04-16
ZA202101433B (en) 2022-07-27
JP2021536253A (ja) 2021-12-27
US20210324438A1 (en) 2021-10-21
EP3847184A1 (en) 2021-07-14
AU2019358330B2 (en) 2024-08-15
WO2020074977A9 (en) 2020-08-06
WO2020074977A1 (en) 2020-04-16
AU2019358330A1 (en) 2021-04-08

Similar Documents

Publication Publication Date Title
MX2018016114A (es) Una novedosa fructosa-6-fosfato-3-epimerasa termoestable y un procedimiento para producir alulosa mediante el uso de la misma.
MX2021002672A (es) Proceso mejorado para la preparación de proteína recombinante.
ZA202101376B (en) Tertiary amino lipidated cationic peptides for nucleic acid delivery
RU2014132431A (ru) Микроорганизм, способный продуцировать l- аминокислоту и способ получения l-аминокислоты с применением этого микроорганизма
NZ628126A (en) Histidyl-trna synthetases for treating autoimmune and inflammatory diseases
IN2012DN01652A (es)
MX2019000082A (es) Una novedosa tagatosa-6-fosfato fosfatasa termoestable y un procedimiento para producir tagatosa usando la misma.
MY176331A (en) Carbohydrate degrading polypeptide and uses thereof
PL2041276T3 (pl) Sposób wytwarzania L-aminokwasów za pomocą mutantów genu gltA kodującego syntazę cytranianową
NZ705575A (en) Recombinant clostridium botulinum neurotoxins
MY159074A (en) Polypeptide having d-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of d-lactic acid
MX2017010359A (es) Cadena alfa del receptor de inmunoglobulina e (ige) de alta afinidad de fusion de fragmento cristalizable (fc).
NZ629843A (en) Identification of channelrhodopsin-2 (chop2) mutations and methods of use
AU2018280528A8 (en) Method for improving transformation efficiency of plant and method for transforming plant
MX2019003360A (es) Vectores de expresion para una secrecion de proteina mejorada.
WO2016130628A8 (en) Griffithsin mutants
RU2014144881A (ru) Способ экспрессии полипептидов с применением модифицированных нуклеиновых кислот
PH12017502323A1 (en) Novel xylanase
NZ586074A (en) LCLRP peptides, constructs and uses thereof
MX2021001890A (es) Metodos de produccion para vectores virales.
NZ709120A (en) Beta-hexosyl-transferases and uses thereof
IN2014KN00866A (es)
WO2023034959A3 (en) Polypeptides and methods for modifying nucleic acids
AU2014370874A1 (en) Improved beta-fructofuranosidase
MY195997A (en) Recombinant Gene