MX2020014328A - Proceso para producir estructuras de colageno humano con caracteristicas controladas. - Google Patents
Proceso para producir estructuras de colageno humano con caracteristicas controladas.Info
- Publication number
- MX2020014328A MX2020014328A MX2020014328A MX2020014328A MX2020014328A MX 2020014328 A MX2020014328 A MX 2020014328A MX 2020014328 A MX2020014328 A MX 2020014328A MX 2020014328 A MX2020014328 A MX 2020014328A MX 2020014328 A MX2020014328 A MX 2020014328A
- Authority
- MX
- Mexico
- Prior art keywords
- solution
- collagen
- subjecting
- acetic acid
- lyophilization
- Prior art date
Links
- 102000008186 Collagen Human genes 0.000 title abstract 5
- 108010035532 Collagen Proteins 0.000 title abstract 5
- 229920001436 collagen Polymers 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 238000004132 cross linking Methods 0.000 abstract 2
- 238000004108 freeze drying Methods 0.000 abstract 2
- 230000003381 solubilizing effect Effects 0.000 abstract 2
- 102000057297 Pepsin A Human genes 0.000 abstract 1
- 108090000284 Pepsin A Proteins 0.000 abstract 1
- 230000003750 conditioning effect Effects 0.000 abstract 1
- 238000000502 dialysis Methods 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 238000000465 moulding Methods 0.000 abstract 1
- 229940111202 pepsin Drugs 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000002203 pretreatment Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/24—Dialysis ; Membrane extraction
- B01D61/243—Dialysis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/12—Addition of chemical agents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2325/00—Details relating to properties of membranes
- B01D2325/02—Details relating to pores or porosity of the membranes
- B01D2325/0283—Pore size
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Analytical Chemistry (AREA)
- Epidemiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Urology & Nephrology (AREA)
- Water Supply & Treatment (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
Abstract
La invención se refiere a un proceso para producir estructuras de colágeno humano con características controladas, que comprende las etapas: a) Acondicionado de tejido b) Pre-tratamiento; b1) lavados finales con agua destilada; c) extracción por hidrólisis enzimática sometiendo el tejido a una solución de ácido acético con pepsina; d) precipitación, en donde la solución resultante de colágeno se lleva a una concentración alta agregando cloruro de sodio y en donde las fibras recuperadas del tamiz se solubilizan nuevamente en una solución de ácido acético; e) diálisis dinámica para purificar la solución del exceso de sal presente en la solución de colágeno; f) liofilización a -40°C y presión de vacío de 0.04 mbar (4 Pa); g) moldeado o segunda liofilización, en donde se elige una concentración y se solubiliza el colágeno en una solución de ácido acético; colocarlo en moldes para generar la estructura deseada y nuevamente liofilizar; h) entrecruzamiento, en donde las piezas de colágeno son sometidas a una atmosfera de vapor de formaldehído en un aparato de entrecruzamiento e i) Prensado en donde la estructura de colágeno es sometida a una fuerza mecánica de 400 -5000 N compactando a un valor de 0.01 - 10 mm aumentando su densidad fibrilar.
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2020014328A MX2020014328A (es) | 2020-12-18 | 2020-12-18 | Proceso para producir estructuras de colageno humano con caracteristicas controladas. |
EP21907196.6A EP4265635A1 (en) | 2020-12-18 | 2021-03-11 | Method for producing human collagen structures with controlled characteristics |
PCT/MX2021/000007 WO2022131896A1 (es) | 2020-12-18 | 2021-03-11 | Proceso para producir estructuras de colágeno humano con características controladas |
CN202180094019.5A CN116848132A (zh) | 2020-12-18 | 2021-03-11 | 产生具有受控特性的人胶原蛋白结构的方法 |
KR1020237024409A KR20230131214A (ko) | 2020-12-18 | 2021-03-11 | 제어된 형질을 포함하는 사람 콜라겐 구조체를 생성하는 방법 |
US18/267,990 US20240059756A1 (en) | 2020-12-18 | 2021-03-11 | Method for producing human collagen structures with controlled characteristics |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MX2020014328A MX2020014328A (es) | 2020-12-18 | 2020-12-18 | Proceso para producir estructuras de colageno humano con caracteristicas controladas. |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2020014328A true MX2020014328A (es) | 2022-06-20 |
Family
ID=82057977
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2020014328A MX2020014328A (es) | 2020-12-18 | 2020-12-18 | Proceso para producir estructuras de colageno humano con caracteristicas controladas. |
Country Status (6)
Country | Link |
---|---|
US (1) | US20240059756A1 (es) |
EP (1) | EP4265635A1 (es) |
KR (1) | KR20230131214A (es) |
CN (1) | CN116848132A (es) |
MX (1) | MX2020014328A (es) |
WO (1) | WO2022131896A1 (es) |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1981000963A1 (en) | 1979-10-08 | 1981-04-16 | Pentapharm Ag | Process for preparing a collagen product for medical and cosmetic use |
WO2016071876A1 (es) | 2014-11-06 | 2016-05-12 | Universidad Nacional De Colombia | Procedimiento para la preparación de colágeno tipo 1 y de soportes unidireccionales y multidireccionales que lo contienen |
CN107236777A (zh) * | 2017-06-21 | 2017-10-10 | 兰溪市沉默生物科技有限公司 | 从鲫鱼鱼皮中提取胶原蛋白的方法 |
CN110564802A (zh) * | 2019-09-30 | 2019-12-13 | 南宁学院 | 一种耗牛跟腱骨胶原蛋白的提取方法 |
-
2020
- 2020-12-18 MX MX2020014328A patent/MX2020014328A/es unknown
-
2021
- 2021-03-11 US US18/267,990 patent/US20240059756A1/en active Pending
- 2021-03-11 EP EP21907196.6A patent/EP4265635A1/en active Pending
- 2021-03-11 CN CN202180094019.5A patent/CN116848132A/zh active Pending
- 2021-03-11 KR KR1020237024409A patent/KR20230131214A/ko active Search and Examination
- 2021-03-11 WO PCT/MX2021/000007 patent/WO2022131896A1/es active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2022131896A1 (es) | 2022-06-23 |
KR20230131214A (ko) | 2023-09-12 |
US20240059756A1 (en) | 2024-02-22 |
CN116848132A (zh) | 2023-10-03 |
EP4265635A1 (en) | 2023-10-25 |
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