MX2020012669A - Procedimiento in vitro para monitorear la carga de patogenos en una poblacion animal. - Google Patents

Procedimiento in vitro para monitorear la carga de patogenos en una poblacion animal.

Info

Publication number
MX2020012669A
MX2020012669A MX2020012669A MX2020012669A MX2020012669A MX 2020012669 A MX2020012669 A MX 2020012669A MX 2020012669 A MX2020012669 A MX 2020012669A MX 2020012669 A MX2020012669 A MX 2020012669A MX 2020012669 A MX2020012669 A MX 2020012669A
Authority
MX
Mexico
Prior art keywords
sample material
pathogen
monitoring
vitro method
nucleic acid
Prior art date
Application number
MX2020012669A
Other languages
English (en)
Inventor
Stefan Pelzer
Frank Thiemann
Monika Flügel
Florian Böhl
Andreas Kappel
Emeka Ignatius Igwe
Michelle Dargatz
Petra Herzog
Jan- Hinnerk JARCK
Original Assignee
Evonik Operations Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Operations Gmbh filed Critical Evonik Operations Gmbh
Publication of MX2020012669A publication Critical patent/MX2020012669A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un procedimiento in vitro para monitorear la carga de al menos un patógeno en una población aviar, comprendiendo el procedimiento las siguientes etapas: recoger y agrupar material de muestra de excrementos provenientes de una población aviar; homogeneizar el material de muestra agrupado obtenido en la etapa (a); diluir y opcionalmente estabilizar el material de muestra agrupado obtenido en la etapa (b) con una solución tampón acuosa; lisar el material celular contenido en el material de muestra diluido obtenido en la etapa (c); aislar el material de ácido nucleico del material de muestra lisado de la etapa (d); detectar y cuantificar al menos un gen diana específico de patógeno, o un fragmento funcional del mismo, contenido en el aislado de ácido nucleico obtenido en la etapa (e); repetir las etapas (a) a (f) en puntos consecutivos en el tiempo; y observar las alteraciones en la cantidad del al menos un gen diana específico de patógeno a lo largo del tiempo.
MX2020012669A 2018-05-29 2019-05-27 Procedimiento in vitro para monitorear la carga de patogenos en una poblacion animal. MX2020012669A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18174689 2018-05-29
PCT/EP2019/063608 WO2019228961A1 (en) 2018-05-29 2019-05-27 In vitro method for monitoring the pathogen load in an animal population

Publications (1)

Publication Number Publication Date
MX2020012669A true MX2020012669A (es) 2021-02-09

Family

ID=62630907

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020012669A MX2020012669A (es) 2018-05-29 2019-05-27 Procedimiento in vitro para monitorear la carga de patogenos en una poblacion animal.

Country Status (9)

Country Link
US (1) US20210207193A1 (es)
EP (1) EP3802860A1 (es)
KR (1) KR20210014136A (es)
CN (1) CN112166198A (es)
AR (1) AR119648A1 (es)
BR (1) BR112020024115A2 (es)
CA (1) CA3101674A1 (es)
MX (1) MX2020012669A (es)
WO (1) WO2019228961A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2944359T3 (es) 2017-05-12 2023-06-20 Evonik Operations Gmbh Procedimiento de detección de enfermedades inducidas por C. perfringens en una bandada de aves

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040101860A1 (en) * 2002-11-27 2004-05-27 Jones Alison M. Predicting animal performance
PT2164862E (pt) * 2007-06-08 2015-10-29 Australian Poultry Crc Pty Ltd Toxina de clostrídio netb
CA2874191A1 (en) * 2012-05-23 2013-11-28 University Of Guelph A novel toxin in type a clostridium perfringens
EP3112474A1 (en) * 2015-06-29 2017-01-04 Evonik Degussa GmbH Method of detecting avian necrotic enteritis
ES2944359T3 (es) * 2017-05-12 2023-06-20 Evonik Operations Gmbh Procedimiento de detección de enfermedades inducidas por C. perfringens en una bandada de aves

Also Published As

Publication number Publication date
KR20210014136A (ko) 2021-02-08
EP3802860A1 (en) 2021-04-14
WO2019228961A1 (en) 2019-12-05
BR112020024115A2 (pt) 2021-03-09
CN112166198A (zh) 2021-01-01
AR119648A1 (es) 2022-01-05
CA3101674A1 (en) 2019-12-05
US20210207193A1 (en) 2021-07-08

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