MX2015013735A - Ensayo biologico de peptidoglicanos. - Google Patents
Ensayo biologico de peptidoglicanos.Info
- Publication number
- MX2015013735A MX2015013735A MX2015013735A MX2015013735A MX2015013735A MX 2015013735 A MX2015013735 A MX 2015013735A MX 2015013735 A MX2015013735 A MX 2015013735A MX 2015013735 A MX2015013735 A MX 2015013735A MX 2015013735 A MX2015013735 A MX 2015013735A
- Authority
- MX
- Mexico
- Prior art keywords
- sample
- pgn
- reporter gene
- peptidoglycans
- tlr2
- Prior art date
Links
- 238000004166 bioassay Methods 0.000 title 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 4
- 102000008228 Toll-like receptor 2 Human genes 0.000 abstract 3
- 108010060888 Toll-like receptor 2 Proteins 0.000 abstract 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 2
- 239000008103 glucose Substances 0.000 abstract 2
- 229920000642 polymer Polymers 0.000 abstract 2
- 230000003113 alkalizing effect Effects 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000010170 biological method Methods 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 238000010790 dilution Methods 0.000 abstract 1
- 239000012895 dilution Substances 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 102000034287 fluorescent proteins Human genes 0.000 abstract 1
- 108091006047 fluorescent proteins Proteins 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000019491 signal transduction Effects 0.000 abstract 1
- 238000000527 sonication Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4722—Proteoglycans, e.g. aggreccan
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
- G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se refiere a un método biológico para someter a ensayo los peptidoglicanos (PGN) en una muestra, en particular una muestra de polímeros de glucosa. El ensayo de PGN incluye: a) tratar la muestra de polímeros de glucosa mediante sonicación, calentamiento y/o alcalinización; b) poner en contacto la muestra tratada o una dilución de esta con una célula recombinante que exprese un TLR2 exógeno (receptor de tipo Toll 2) y un gen indicador que dependa directamente de la ruta de señalización asociada con el TLR2. El gen indicador codifica una proteína coloreada o fluorescente o una proteína cuya actividad es medible con o sin un sustrato; c) medir la señal del gen indicador; y d) determinar la cantidad de PGN en la muestra utilizando una curva patrón de la correlación entre la cantidad de PGN y la intensidad de la señal del gen indicador.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1352732 | 2013-03-26 | ||
FR1357197 | 2013-07-22 | ||
PCT/EP2014/055888 WO2014154651A1 (fr) | 2013-03-26 | 2014-03-25 | Dosage biologique des peptidoglycanes |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2015013735A true MX2015013735A (es) | 2016-06-21 |
Family
ID=50397130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015013735A MX2015013735A (es) | 2013-03-26 | 2014-03-25 | Ensayo biologico de peptidoglicanos. |
Country Status (13)
Country | Link |
---|---|
US (2) | US9857355B2 (es) |
EP (1) | EP2979091B1 (es) |
JP (1) | JP6454682B2 (es) |
KR (1) | KR102318190B1 (es) |
CN (1) | CN105122059B (es) |
AU (1) | AU2014243145B2 (es) |
BR (1) | BR112015024577A2 (es) |
CA (1) | CA2904965C (es) |
ES (1) | ES2679493T3 (es) |
MX (1) | MX2015013735A (es) |
RU (1) | RU2680885C2 (es) |
SG (1) | SG11201507708WA (es) |
WO (1) | WO2014154651A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2694961B2 (fr) * | 2011-04-08 | 2022-01-05 | Roquette Frères | Methodes de detection de contaminants dans des solutions contenants des polymeres de glucose |
MX2016009774A (es) | 2014-02-07 | 2016-11-14 | Roquette Freres | Ensayo biologico de peptidoglicanos. |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2077723C1 (ru) * | 1993-02-11 | 1997-04-20 | Научно-исследовательский конструкторско-технологический институт биологически активных веществ Научно-производственного объединения "Вектор" | Способ определения липополисахаридов |
US7118857B2 (en) | 2004-02-27 | 2006-10-10 | Baxter International Inc. | Methods and compositions for detection of microbial contaminants in peritoneal dialysis solutions |
JP4455933B2 (ja) * | 2004-05-17 | 2010-04-21 | 株式会社タクマ | 汚泥の水素発酵方法 |
US20090239819A1 (en) * | 2008-03-20 | 2009-09-24 | Run Wang | Peritoneal dialysis solution test method |
GB2481267B (en) * | 2010-06-15 | 2016-04-20 | Univ Leicester | Assay for determining the risk of a toll-like receptor containing foodstuff for causing systemic inflammation in vivo |
EP2694961B2 (fr) * | 2011-04-08 | 2022-01-05 | Roquette Frères | Methodes de detection de contaminants dans des solutions contenants des polymeres de glucose |
JP5821358B2 (ja) * | 2011-07-20 | 2015-11-24 | ソニー株式会社 | 核酸抽出方法及び核酸抽出用カートリッジ |
JP6244356B2 (ja) * | 2012-05-29 | 2017-12-06 | ロケット フレールRoquette Freres | グルコースポリマーおよびグルコースポリマー加水分解物の製造回路の除染方法 |
-
2014
- 2014-03-25 EP EP14714218.6A patent/EP2979091B1/fr not_active Revoked
- 2014-03-25 MX MX2015013735A patent/MX2015013735A/es active IP Right Grant
- 2014-03-25 JP JP2016504619A patent/JP6454682B2/ja not_active Expired - Fee Related
- 2014-03-25 KR KR1020157026299A patent/KR102318190B1/ko active IP Right Grant
- 2014-03-25 BR BR112015024577A patent/BR112015024577A2/pt not_active Application Discontinuation
- 2014-03-25 SG SG11201507708WA patent/SG11201507708WA/en unknown
- 2014-03-25 CN CN201480018300.0A patent/CN105122059B/zh active Active
- 2014-03-25 RU RU2015145785A patent/RU2680885C2/ru active
- 2014-03-25 ES ES14714218.6T patent/ES2679493T3/es active Active
- 2014-03-25 CA CA2904965A patent/CA2904965C/fr active Active
- 2014-03-25 WO PCT/EP2014/055888 patent/WO2014154651A1/fr active Application Filing
- 2014-03-25 US US14/779,353 patent/US9857355B2/en active Active
- 2014-03-25 AU AU2014243145A patent/AU2014243145B2/en not_active Ceased
-
2017
- 2017-11-02 US US15/801,854 patent/US20180188233A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
CA2904965C (fr) | 2021-11-16 |
US20180188233A1 (en) | 2018-07-05 |
JP6454682B2 (ja) | 2019-01-16 |
SG11201507708WA (en) | 2015-10-29 |
CN105122059A (zh) | 2015-12-02 |
KR20150135302A (ko) | 2015-12-02 |
US20160054301A1 (en) | 2016-02-25 |
EP2979091B1 (fr) | 2018-04-25 |
RU2015145785A (ru) | 2017-05-02 |
AU2014243145B2 (en) | 2020-04-02 |
KR102318190B1 (ko) | 2021-10-28 |
EP2979091A1 (fr) | 2016-02-03 |
ES2679493T3 (es) | 2018-08-27 |
RU2680885C2 (ru) | 2019-02-28 |
CA2904965A1 (fr) | 2014-10-02 |
US9857355B2 (en) | 2018-01-02 |
AU2014243145A8 (en) | 2015-10-15 |
CN105122059B (zh) | 2019-04-30 |
WO2014154651A1 (fr) | 2014-10-02 |
BR112015024577A2 (pt) | 2017-07-18 |
AU2014243145A1 (en) | 2015-10-08 |
JP2016514468A (ja) | 2016-05-23 |
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