MX2020005258A - Oxidacion de sesquiterpenos catalizada por monooxigenasa de citocromo p450. - Google Patents

Oxidacion de sesquiterpenos catalizada por monooxigenasa de citocromo p450.

Info

Publication number
MX2020005258A
MX2020005258A MX2020005258A MX2020005258A MX2020005258A MX 2020005258 A MX2020005258 A MX 2020005258A MX 2020005258 A MX2020005258 A MX 2020005258A MX 2020005258 A MX2020005258 A MX 2020005258A MX 2020005258 A MX2020005258 A MX 2020005258A
Authority
MX
Mexico
Prior art keywords
cytochrome
nucleic acid
sesquiterpenes
terpene
provides
Prior art date
Application number
MX2020005258A
Other languages
English (en)
Inventor
Michel Schalk
Fabienne Deguerry
Letizia ROCCI
Original Assignee
Firmenich & Cie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Firmenich & Cie filed Critical Firmenich & Cie
Publication of MX2020005258A publication Critical patent/MX2020005258A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15006—Cholesterol monooxygenase (side-chain-cleaving) (1.14.15.6), i.e. cytochrome P450scc

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención proporciona el ácido nucleico y las secuencias de aminoácidos de un citocromo P450 capaz de oxidar moléculas de terpeno. También proporciona los métodos para oxidar las moléculas de terpeno, que comprenden poner en contacto el citocromo P450 con la molécula de terpeno destinada a oxidarse. En particular, el método puede llevarse a cabo in vitro o in vivo para producir moléculas de terpeno oxidadas, que pueden usarse en diferentes campos técnicos tales como, por ejemplo, perfumería y saborizantes. La presente invención también proporciona un vector de expresión que contiene el ácido nucleico. Un organismo hospedero no humano o una célula transformada con el ácido nucleico también es un objeto de la invención.
MX2020005258A 2018-01-18 2019-01-16 Oxidacion de sesquiterpenos catalizada por monooxigenasa de citocromo p450. MX2020005258A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18152363 2018-01-18
PCT/EP2019/051070 WO2019141739A1 (en) 2018-01-18 2019-01-16 Cytochrome p450 monooxygenase catalyzed oxidation of sesquiterpenes

Publications (1)

Publication Number Publication Date
MX2020005258A true MX2020005258A (es) 2020-08-24

Family

ID=61187066

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020005258A MX2020005258A (es) 2018-01-18 2019-01-16 Oxidacion de sesquiterpenos catalizada por monooxigenasa de citocromo p450.

Country Status (7)

Country Link
US (1) US20230151393A1 (es)
EP (1) EP3697898A1 (es)
JP (1) JP7431733B2 (es)
CN (1) CN111527203B (es)
BR (1) BR112020010924A2 (es)
MX (1) MX2020005258A (es)
WO (1) WO2019141739A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114621934B (zh) * 2020-12-10 2023-11-24 中国科学院大连化学物理研究所 一种p450还原酶及其应用
CN114639442B (zh) * 2022-03-30 2024-01-30 中国农业科学院农业基因组研究所 一种基于单核苷酸多态性预测开放阅读框的方法及系统
WO2024132991A1 (en) * 2022-12-22 2024-06-27 Isobionics B.V. Recombinant production of zizaene and other sesquiterpenesformed by conversion of the bisabolyl cation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130167263A1 (en) * 2007-10-30 2013-06-27 Monsanto Technology Llc Nucleic acid molecules and other molecules associated with plants and uses thereof
DE19931847A1 (de) 1999-07-09 2001-01-11 Basf Ag Immobilisierte Lipase
DE10019377A1 (de) 2000-04-19 2001-10-25 Basf Ag Verfahren zur Immobilisierung von biologisch aktiven Stoffen auf Trägermaterialien und Verwendung der mit biologisch aktiven Stoffen geträgerten Materialien für chirale Synthesen
DE10019380A1 (de) 2000-04-19 2001-10-25 Basf Ag Verfahren zur Herstellung von kovalent gebundenen biologisch aktiven Stoffen an Polyurethanschaumstoffen sowie Verwendung der geträgerten Polyurethanschaumstoffe für chirale Synthesen
EP1893753B1 (en) 2005-06-17 2015-12-23 Firmenich Sa Novel sesquiterpene synthases and methods of their use
AU2007299219A1 (en) * 2006-04-05 2008-03-27 Metanomics Gmbh Process for the production of a fine chemical
US8299318B2 (en) * 2007-07-05 2012-10-30 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
CN104017794B (zh) * 2009-05-20 2019-04-23 弗门尼舍有限公司 用于生产(+)-客烯的方法
RU2625224C2 (ru) * 2011-11-01 2017-07-12 Фирмениш Са Цитохром p450 и его применение для энзиматического окисления терпенов
CN112176000B (zh) 2013-09-19 2024-09-10 弗门尼舍有限公司 用于生产芳香醇的方法
US10934564B2 (en) * 2014-08-21 2021-03-02 Manus Bio Inc. Methods for production of oxygenated terpenes
EP3488008A2 (en) * 2016-07-20 2019-05-29 Firmenich SA Vetiver

Also Published As

Publication number Publication date
EP3697898A1 (en) 2020-08-26
CN111527203A (zh) 2020-08-11
WO2019141739A1 (en) 2019-07-25
BR112020010924A2 (pt) 2020-11-17
US20230151393A1 (en) 2023-05-18
JP7431733B2 (ja) 2024-02-15
CN111527203B (zh) 2024-09-06
JP2021511008A (ja) 2021-05-06

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