MX2020001430A - Metodo para detectar un microorganismo en una muestra. - Google Patents

Metodo para detectar un microorganismo en una muestra.

Info

Publication number
MX2020001430A
MX2020001430A MX2020001430A MX2020001430A MX2020001430A MX 2020001430 A MX2020001430 A MX 2020001430A MX 2020001430 A MX2020001430 A MX 2020001430A MX 2020001430 A MX2020001430 A MX 2020001430A MX 2020001430 A MX2020001430 A MX 2020001430A
Authority
MX
Mexico
Prior art keywords
sample
microorganism
detecting
microorganisms
dna
Prior art date
Application number
MX2020001430A
Other languages
English (en)
Inventor
Tamryn Jo Hassel
Ruth Catherine Massey
Leann Francis Bacon
Original Assignee
Corsham Science Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Corsham Science Ltd filed Critical Corsham Science Ltd
Publication of MX2020001430A publication Critical patent/MX2020001430A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención proporciona un método para detectar un microorganismo en una muestra, el método comprende: a) filtrar una muestra a través de un filtro para atrapar cualquier microorganismo presente en la muestra; b) tratar el filtro para liberar el material genómico o ADN de los microorganismos atrapados; c) amplificar el material genómico o el ADN liberado de los microorganismos atrapados; y d) identificar las regiones específicas del material genómico o ADN para determinar la presencia, identificar las especies o cuantificar el número aproximado de microorganismos atrapados.
MX2020001430A 2017-08-08 2018-08-08 Metodo para detectar un microorganismo en una muestra. MX2020001430A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17185252.8A EP3441477A1 (en) 2017-08-08 2017-08-08 Method for detecting a microorganism in a sample
PCT/EP2018/071463 WO2019030261A1 (en) 2017-08-08 2018-08-08 METHOD FOR DETECTION OF A MICROORGANISM IN A SAMPLE

Publications (1)

Publication Number Publication Date
MX2020001430A true MX2020001430A (es) 2020-08-06

Family

ID=59569187

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020001430A MX2020001430A (es) 2017-08-08 2018-08-08 Metodo para detectar un microorganismo en una muestra.

Country Status (16)

Country Link
US (1) US11390925B2 (es)
EP (2) EP3441477A1 (es)
JP (1) JP7203108B2 (es)
KR (1) KR20200035123A (es)
CN (1) CN111183231A (es)
AU (1) AU2018314766A1 (es)
BR (1) BR112020002470A2 (es)
CA (1) CA3071929A1 (es)
DK (1) DK3523442T3 (es)
ES (1) ES2876239T3 (es)
HU (1) HUE054490T2 (es)
MX (1) MX2020001430A (es)
PL (1) PL3523442T3 (es)
PT (1) PT3523442T (es)
WO (1) WO2019030261A1 (es)
ZA (1) ZA202000813B (es)

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0461477A1 (en) * 1990-05-31 1991-12-18 Shimadzu Corporation Method of bacterial examination and apparatus therefor
WO1997008293A1 (en) * 1995-08-25 1997-03-06 Scientific Generics Limited Release of intracellular material
AU2007201860A1 (en) * 2000-07-28 2007-05-17 University Of Sydney A method of detecting microorganisms
JP4373947B2 (ja) * 2004-05-04 2009-11-25 ミリポア・コーポレイション 微生物を計数し特定するための方法
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
US8835109B2 (en) 2007-11-16 2014-09-16 E. I. Du Pont De Nemours And Company Method for detection and/or analysis of yeast and mold in filterable liquids
WO2010111509A1 (en) * 2009-03-25 2010-09-30 Life Technologies Corporation Discriminatory positive/extraction control dna
US9044694B2 (en) * 2010-09-01 2015-06-02 Life Technologies Corporation Device for capture and lysis of microorganisms from liquids
US9932620B2 (en) * 2012-05-21 2018-04-03 Celsis Ltd. Methods, devices, and systems of detecting microorganisms
JP6075519B1 (ja) * 2015-05-29 2017-02-08 株式会社村田製作所 抽出方法、分析方法、抽出装置および分析装置
WO2017116694A1 (en) * 2015-12-29 2017-07-06 3M Innovative Properties Company Method and system for detecting microorganisms in large-volume samples

Also Published As

Publication number Publication date
AU2018314766A1 (en) 2020-03-05
BR112020002470A2 (pt) 2020-08-18
ZA202000813B (en) 2021-10-27
CN111183231A (zh) 2020-05-19
US11390925B2 (en) 2022-07-19
EP3523442A1 (en) 2019-08-14
DK3523442T3 (da) 2021-05-31
US20200248238A1 (en) 2020-08-06
WO2019030261A1 (en) 2019-02-14
JP7203108B2 (ja) 2023-01-12
PL3523442T3 (pl) 2021-10-18
CA3071929A1 (en) 2019-02-14
HUE054490T2 (hu) 2021-09-28
EP3523442B1 (en) 2021-03-03
EP3441477A1 (en) 2019-02-13
ES2876239T3 (es) 2021-11-12
KR20200035123A (ko) 2020-04-01
PT3523442T (pt) 2021-06-02
JP2020529869A (ja) 2020-10-15

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