MX2019001354A - Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado. - Google Patents

Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado.

Info

Publication number
MX2019001354A
MX2019001354A MX2019001354A MX2019001354A MX2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A
Authority
MX
Mexico
Prior art keywords
particle content
state imaging
quantitative measurement
hydrated state
vlps
Prior art date
Application number
MX2019001354A
Other languages
English (en)
Inventor
Haag Lars
Ryner Martin
COLOMB-DELSUC Mathieu
Nordström Rickard
Original Assignee
Intelligent Virus Imaging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intelligent Virus Imaging Inc filed Critical Intelligent Virus Imaging Inc
Publication of MX2019001354A publication Critical patent/MX2019001354A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Signal Processing (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Computer Vision & Pattern Recognition (AREA)

Abstract

El método sirve para la medición cuantitativa de contenido de partículas mediante el uso de generación de imágenes en estado hidratado tal como CryoTEM. Se provee una muestra (100) de partículas tipo virus (VLP) o partículas de virus. Preferiblemente, la muestra (100) se congela rápidamente en un líquido criogénico a una temperatura criogénica. Mientras que, en la temperatura criogénica, el contenido de partículas de cada VLP en la muestra congelada se observa en el CryoTEM. Una cantidad de contenido de partículas de los VLP se determina para evaluar si los VLP están vacíos o no.
MX2019001354A 2017-03-02 2018-01-25 Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado. MX2019001354A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762465981P 2017-03-02 2017-03-02
PCT/US2018/015263 WO2018160298A1 (en) 2017-03-02 2018-01-25 Method of quantitative measurement of particle content using hydrated state imaging

Publications (1)

Publication Number Publication Date
MX2019001354A true MX2019001354A (es) 2019-06-03

Family

ID=63370196

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019001354A MX2019001354A (es) 2017-03-02 2018-01-25 Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado.

Country Status (17)

Country Link
US (2) US11125672B2 (es)
EP (1) EP3589643A4 (es)
JP (1) JP6865769B2 (es)
KR (1) KR102149623B1 (es)
CN (1) CN108884133A (es)
AU (1) AU2018228346A1 (es)
BR (1) BR112019001151A2 (es)
CA (1) CA3015803A1 (es)
CL (1) CL2018002809A1 (es)
EA (1) EA037471B1 (es)
HK (1) HK1256983A1 (es)
IL (1) IL263737A (es)
MA (1) MA44418B1 (es)
MX (1) MX2019001354A (es)
SG (1) SG11201900270YA (es)
WO (1) WO2018160298A1 (es)
ZA (1) ZA201900102B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté
CN112183433B (zh) * 2020-10-12 2024-02-23 水木未来(北京)科技有限公司 一种实心和空心病毒颗粒的表征量化方法
CN113009184A (zh) * 2021-04-20 2021-06-22 浙江科技学院 一种基于低共熔溶剂制备冷冻电镜检测样品的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8712140B2 (en) * 2005-04-15 2014-04-29 Intelligent Virus Imaging Inc. Method of analyzing cell structures and their components
RU2461063C2 (ru) * 2007-05-30 2012-09-10 Интеллигент Вирус Имагинг Инк. Способ подсчета и сегментации частиц вирусов на изображении
US20130191037A1 (en) * 2011-07-26 2013-07-25 Nanoimaging Services Characterization of particulates using electron microscopy and image processing methods
US10192025B2 (en) * 2013-07-08 2019-01-29 Novartis Ag Rotavirus particles with chimeric surface proteins
ES2705574T3 (es) * 2013-10-22 2019-03-26 Hoffmann La Roche Procedimientos para medir partículas de virus libres de células a partir de gotas de sangre seca
JP6452630B2 (ja) * 2014-01-29 2019-01-16 国立研究開発法人科学技術振興機構 含水状態の生物試料の電子顕微鏡観察用保護剤、電子顕微鏡観察用キット、電子顕微鏡による観察、診断、評価、定量の方法並びに試料台
CN104537713B (zh) * 2015-01-05 2017-10-03 清华大学 一种新型三维重构系统
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Also Published As

Publication number Publication date
EP3589643A1 (en) 2020-01-08
MA44418A1 (fr) 2020-04-30
US20190331584A1 (en) 2019-10-31
IL263737A (en) 2019-01-31
US20210341372A1 (en) 2021-11-04
CA3015803A1 (en) 2018-09-02
AU2018228346A1 (en) 2018-10-04
EA037471B1 (ru) 2021-03-31
MA44418B1 (fr) 2021-11-30
KR102149623B1 (ko) 2020-08-28
EA201891845A1 (ru) 2020-03-02
US11125672B2 (en) 2021-09-21
BR112019001151A2 (pt) 2019-04-30
SG11201900270YA (en) 2019-02-27
EP3589643A4 (en) 2020-12-16
ZA201900102B (en) 2019-09-25
JP2020513230A (ja) 2020-05-14
KR20190038541A (ko) 2019-04-08
HK1256983A1 (zh) 2019-10-11
CL2018002809A1 (es) 2019-01-18
CN108884133A (zh) 2018-11-23
JP6865769B2 (ja) 2021-04-28
WO2018160298A1 (en) 2018-09-07

Similar Documents

Publication Publication Date Title
MX2019001354A (es) Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado.
MX2018016258A (es) Metodo de diferenciacion mejorada.
WO2016015058A3 (en) Methods of determining tissues and/or cell types giving rise to cell-free dna, and methods of identifying a disease or disorder using same
WO2015109323A3 (en) Systems and methods for three-dimensional imaging
PH12014502092B1 (en) Stabilizing composition for biological materials
WO2016087873A3 (en) Thawing methods and apparatus
CA3010454A1 (fr) Systeme et procede de mesure d'une propriete d'ecoulement d'un fluide au sein d'un milieu poreux
MX2021013459A (es) Medicion rapida de la tasa de sedimentacion de los componentes sanguineos formados a partir de peque?os volumenes de muestra.
WO2014151091A3 (en) Compositions of and methods for cancellous bone matrix
EA201691742A1 (ru) Препараты из кластеров стенок паренхимных клеток растений
WO2015140797A3 (en) Method and system for determining package integrity
WO2014179539A3 (en) Apparatus, system, and method for collecting a target material
JP2015057591A5 (es)
WO2015123192A3 (en) Universal system, method and solution for the acceleration of the process of fixing, dehydrating and clearing the structure of biological tissue
GB2514741A (en) Composition for use as a paint binder
EP2953095A3 (en) Image processing apparatus, image processing system, image processing method, and storage medium
MX2017008340A (es) Metodos para determinar la inestabilidad de la columna y para eliminar el impacto del esfuerzo del paciente sobre determinaciones de estabilidad.
RU2014127477A (ru) Способ консервации биоматериала
BR112018008663A2 (pt) aparato para armazenar materiais orgânicos, filtro para filtrar líquidos e método para coletar e armazenar material orgânico de múltiplos fornecedores para processá-lo em material de consumo
RU2017130128A (ru) Материалы и определение их характеристик при канальном гидроразрыве пласта
EA201690304A1 (ru) Композитное сырье для извлечения углеводородов из углеводородсодержащего материала
CH708198A8 (de) System zur Herstellung eines Knochenersatzmaterials, umfassend einen Behälter und ein Rückhalteelement.
MX2016009952A (es) Proteinas y antigenos del virus de diarrea epidemica porcino (pedv).
EP3856240A4 (en) COMPOSITIONS AND METHODS OF MANUFACTURE AND USE OF VIRUS-LIKE PARTICLES (VLPS)
MX2015010566A (es) Metodos para la liberacion de las particulas similares a virus.