MX2019001354A - Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado. - Google Patents

Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado.

Info

Publication number
MX2019001354A
MX2019001354A MX2019001354A MX2019001354A MX2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A MX 2019001354 A MX2019001354 A MX 2019001354A
Authority
MX
Mexico
Prior art keywords
particle content
state imaging
quantitative measurement
hydrated state
vlps
Prior art date
Application number
MX2019001354A
Other languages
English (en)
Inventor
Haag Lars
Ryner Martin
COLOMB-DELSUC Mathieu
Nordström Rickard
Original Assignee
Intelligent Virus Imaging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intelligent Virus Imaging Inc filed Critical Intelligent Virus Imaging Inc
Publication of MX2019001354A publication Critical patent/MX2019001354A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Signal Processing (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Multimedia (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

El método sirve para la medición cuantitativa de contenido de partículas mediante el uso de generación de imágenes en estado hidratado tal como CryoTEM. Se provee una muestra (100) de partículas tipo virus (VLP) o partículas de virus. Preferiblemente, la muestra (100) se congela rápidamente en un líquido criogénico a una temperatura criogénica. Mientras que, en la temperatura criogénica, el contenido de partículas de cada VLP en la muestra congelada se observa en el CryoTEM. Una cantidad de contenido de partículas de los VLP se determina para evaluar si los VLP están vacíos o no.
MX2019001354A 2017-03-02 2018-01-25 Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado. MX2019001354A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762465981P 2017-03-02 2017-03-02
PCT/US2018/015263 WO2018160298A1 (en) 2017-03-02 2018-01-25 Method of quantitative measurement of particle content using hydrated state imaging

Publications (1)

Publication Number Publication Date
MX2019001354A true MX2019001354A (es) 2019-06-03

Family

ID=63370196

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019001354A MX2019001354A (es) 2017-03-02 2018-01-25 Metodo de medicion cuantitativa de contenido de particulas mediante el uso de generacion de imagenes en estado hidratado.

Country Status (17)

Country Link
US (2) US11125672B2 (es)
EP (1) EP3589643A4 (es)
JP (1) JP6865769B2 (es)
KR (1) KR102149623B1 (es)
CN (1) CN108884133A (es)
AU (1) AU2018228346A1 (es)
BR (1) BR112019001151A2 (es)
CA (1) CA3015803A1 (es)
CL (1) CL2018002809A1 (es)
EA (1) EA037471B1 (es)
HK (1) HK1256983A1 (es)
IL (1) IL263737A (es)
MA (1) MA44418B1 (es)
MX (1) MX2019001354A (es)
SG (1) SG11201900270YA (es)
WO (1) WO2018160298A1 (es)
ZA (1) ZA201900102B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté
CN112183433B (zh) * 2020-10-12 2024-02-23 水木未来(北京)科技有限公司 一种实心和空心病毒颗粒的表征量化方法
CN113009184A (zh) * 2021-04-20 2021-06-22 浙江科技学院 一种基于低共熔溶剂制备冷冻电镜检测样品的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8712140B2 (en) * 2005-04-15 2014-04-29 Intelligent Virus Imaging Inc. Method of analyzing cell structures and their components
MX2009012265A (es) * 2007-05-30 2009-12-01 Intelligent Virus Imaging Inc Un metodo para contar y segmentar particulas virales en una imagen.
US20130191037A1 (en) * 2011-07-26 2013-07-25 Nanoimaging Services Characterization of particulates using electron microscopy and image processing methods
WO2015004158A1 (en) * 2013-07-08 2015-01-15 Novartis Ag Rotavirus particles with chimeric surface proteins
EP3060920B1 (en) * 2013-10-22 2018-11-14 Roche Diagnostics GmbH Methods for measuring cell-free virus particles from dried blood spots
WO2015115502A1 (ja) * 2014-01-29 2015-08-06 独立行政法人科学技術振興機構 含水状態の生物試料の電子顕微鏡観察用保護剤、電子顕微鏡観察用キット、電子顕微鏡による観察、診断、評価、定量の方法並びに試料台
CN104537713B (zh) * 2015-01-05 2017-10-03 清华大学 一种新型三维重构系统
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Also Published As

Publication number Publication date
US11125672B2 (en) 2021-09-21
CL2018002809A1 (es) 2019-01-18
HK1256983A1 (zh) 2019-10-11
JP2020513230A (ja) 2020-05-14
EP3589643A1 (en) 2020-01-08
BR112019001151A2 (pt) 2019-04-30
EP3589643A4 (en) 2020-12-16
JP6865769B2 (ja) 2021-04-28
CA3015803A1 (en) 2018-09-02
SG11201900270YA (en) 2019-02-27
ZA201900102B (en) 2019-09-25
WO2018160298A1 (en) 2018-09-07
MA44418A1 (fr) 2020-04-30
IL263737A (en) 2019-01-31
MA44418B1 (fr) 2021-11-30
CN108884133A (zh) 2018-11-23
US20190331584A1 (en) 2019-10-31
EA037471B1 (ru) 2021-03-31
KR102149623B1 (ko) 2020-08-28
KR20190038541A (ko) 2019-04-08
AU2018228346A1 (en) 2018-10-04
EA201891845A1 (ru) 2020-03-02
US20210341372A1 (en) 2021-11-04

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