EA201891845A1 - Способ количественного измерения содержимого частицы с использованием получения изображения в водосодержащем состоянии - Google Patents

Способ количественного измерения содержимого частицы с использованием получения изображения в водосодержащем состоянии

Info

Publication number
EA201891845A1
EA201891845A1 EA201891845A EA201891845A EA201891845A1 EA 201891845 A1 EA201891845 A1 EA 201891845A1 EA 201891845 A EA201891845 A EA 201891845A EA 201891845 A EA201891845 A EA 201891845A EA 201891845 A1 EA201891845 A1 EA 201891845A1
Authority
EA
Eurasian Patent Office
Prior art keywords
particles
water
vlp
containing state
image
Prior art date
Application number
EA201891845A
Other languages
English (en)
Other versions
EA037471B1 (ru
Inventor
Матье Коломб-Делсак
Ларс Хааг
Рикард Нордстром
Мартин Райнер
Original Assignee
Интеллиджент Вайерэс Имэджинг Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Интеллиджент Вайерэс Имэджинг Инк. filed Critical Интеллиджент Вайерэс Имэджинг Инк.
Publication of EA201891845A1 publication Critical patent/EA201891845A1/ru
Publication of EA037471B1 publication Critical patent/EA037471B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Signal Processing (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Способ предназначен для количественного измерения содержимого частиц с помощью получения изображения в водосодержащем состоянии, такого как крио-ТЭМ. Представляется образец (100) вирусоподобных частиц (VLP) или вирусных частиц. Предпочтительно, чтобы образец (100) быстро замораживался в криогенной жидкости при криогенной температуре. При криогенной температуре содержимое каждой частицы VLP в замороженном образце наблюдается в крио-ТЭМ. Количество содержимого частиц ВЛП определяется для оценки того, являются ли частицы VLP пустыми или нет.
EA201891845A 2017-03-02 2018-01-25 Способ количественного измерения содержимого частицы с использованием получения изображения в водосодержащем состоянии EA037471B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762465981P 2017-03-02 2017-03-02
PCT/US2018/015263 WO2018160298A1 (en) 2017-03-02 2018-01-25 Method of quantitative measurement of particle content using hydrated state imaging

Publications (2)

Publication Number Publication Date
EA201891845A1 true EA201891845A1 (ru) 2020-03-02
EA037471B1 EA037471B1 (ru) 2021-03-31

Family

ID=63370196

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201891845A EA037471B1 (ru) 2017-03-02 2018-01-25 Способ количественного измерения содержимого частицы с использованием получения изображения в водосодержащем состоянии

Country Status (17)

Country Link
US (2) US11125672B2 (ru)
EP (1) EP3589643A4 (ru)
JP (1) JP6865769B2 (ru)
KR (1) KR102149623B1 (ru)
CN (1) CN108884133A (ru)
AU (1) AU2018228346A1 (ru)
BR (1) BR112019001151A2 (ru)
CA (1) CA3015803A1 (ru)
CL (1) CL2018002809A1 (ru)
EA (1) EA037471B1 (ru)
HK (1) HK1256983A1 (ru)
IL (1) IL263737A (ru)
MA (1) MA44418B1 (ru)
MX (1) MX2019001354A (ru)
SG (1) SG11201900270YA (ru)
WO (1) WO2018160298A1 (ru)
ZA (1) ZA201900102B (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté
CN112183433B (zh) * 2020-10-12 2024-02-23 水木未来(北京)科技有限公司 一种实心和空心病毒颗粒的表征量化方法
CN113009184A (zh) * 2021-04-20 2021-06-22 浙江科技学院 一种基于低共熔溶剂制备冷冻电镜检测样品的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8712140B2 (en) * 2005-04-15 2014-04-29 Intelligent Virus Imaging Inc. Method of analyzing cell structures and their components
BRPI0811951A2 (pt) * 2007-05-30 2016-10-04 Intelligent Virus Imagins Inc método de contagem e segmentação de partículas virais em uma imagem.
WO2013016498A1 (en) 2011-07-26 2013-01-31 Nanoimaging Services Characterization of particulates using electron microscopy and image processing methods
CN105518022B (zh) * 2013-07-08 2020-08-18 诺华股份有限公司 具有嵌合表面蛋白的轮状病毒颗粒
CN105659092B (zh) * 2013-10-22 2018-05-25 豪夫迈·罗氏有限公司 测量干血斑中无细胞病毒颗粒的方法
CN105940291B (zh) * 2014-01-29 2019-03-08 国立研究开发法人科学技术振兴机构 含水状态的生物试样的电子显微镜观察用保护剂、试剂盒、利用电子显微镜进行观察、诊断、评价、定量的方法以及试样台
CN104537713B (zh) * 2015-01-05 2017-10-03 清华大学 一种新型三维重构系统
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Also Published As

Publication number Publication date
JP6865769B2 (ja) 2021-04-28
SG11201900270YA (en) 2019-02-27
MA44418B1 (fr) 2021-11-30
KR20190038541A (ko) 2019-04-08
EA037471B1 (ru) 2021-03-31
ZA201900102B (en) 2019-09-25
HK1256983A1 (zh) 2019-10-11
BR112019001151A2 (pt) 2019-04-30
MA44418A1 (fr) 2020-04-30
EP3589643A4 (en) 2020-12-16
CA3015803A1 (en) 2018-09-02
CN108884133A (zh) 2018-11-23
US20210341372A1 (en) 2021-11-04
AU2018228346A1 (en) 2018-10-04
CL2018002809A1 (es) 2019-01-18
JP2020513230A (ja) 2020-05-14
IL263737A (en) 2019-01-31
US20190331584A1 (en) 2019-10-31
MX2019001354A (es) 2019-06-03
KR102149623B1 (ko) 2020-08-28
WO2018160298A1 (en) 2018-09-07
US11125672B2 (en) 2021-09-21
EP3589643A1 (en) 2020-01-08

Similar Documents

Publication Publication Date Title
EA201891845A1 (ru) Способ количественного измерения содержимого частицы с использованием получения изображения в водосодержащем состоянии
MX2016009829A (es) Un sistema y metodo para adquisicion de imagen usando formacion de imagenes de alta calidad supervisada.
MX371134B (es) Aparato para la inspeccion optica de pequenos volumenes de muestra liquida y cubetas para las mismas.
MX2021013459A (es) Medicion rapida de la tasa de sedimentacion de los componentes sanguineos formados a partir de peque?os volumenes de muestra.
BR112017008315A2 (pt) aparelho para gerar um meio inalável, método para gerar um meio inalável utilizando um aparelho e cartucho
MX2015010480A (es) Analisis de imagen y medicion de muestras biologicas.
WO2015145263A8 (en) Method and apparatus for analyzing anomalies in concrete structures
MY187050A (en) Cancer cell-specific antibody, anticancer agent, and cancer testing method
FR3035505B1 (fr) Dispositif d’estimation de la concentration massique de particules fines contenues dans l’air d’une enceinte
JP2016065767A5 (ru)
MX2017005629A (es) Espectrometria de retrodispersion para determinar una concentracion de solidos en un fluido cargado de solidos.
MX2016013453A (es) Inspeccion de contenedores.
MY191701A (en) Method for forming sample addition part of immunochromatographic test device and immunochromatographic test device
WO2014145678A3 (en) System and method for assessing quantities or sizes of lipoprotein particles from lipoprotein particle compositions
WO2016013949A3 (en) Biomimetic gellable colloid for encoding, processing and sectioning histological samples
RU2013127071A (ru) Способ измерения концентрации частиц аэрозоля и устройство для его осуществления
Vidal et al. 2D/3D Quantification of bone morphometric parameter changes using X-ray microtomograpphy with different pixel sizes
EA202192044A3 (ru) Способы и устройства для неразрушающего обнаружения нерастворенных частиц в текучей среде
Sundus et al. Interplay of vitamin D, vitamin B12, homocysteine and bone mineral density in postmenopausal females
JP2015179619A5 (ru)
Kandpal et al. Measurement of drug component distribution and hardness using hyperspectral imaging and NIR spectroscopy
Kawata et al. 3P079 New highly accurate pickup methods, MRA-StoPICK and MRMA-StoPICK methods, for single particle analysis using electron microscope (01E. Protein: Measurement & Analysis, Poster)
Pfeiffer et al. Garnik Asatrian–A Caspian Term for “Mud, Clay, Slime”.... 389 Mohammad Ghanbari–A Note on South Caspian Lās (a)“Stone”.. 393
Xu et al. Effect of different drying methods on the volatile compounds Jinmudan Oolong tea
Als-Nielsen MS89. O02